>SFI165 (SFI165Q) /CSM/SF/SFI1-C/SFI165Q.Seq.d/
XXXXXXXXXXAATTGAAGAAGAGAAAATTAATTTAATTAANCAATTAAAAGAATTAGAAG
ATAANCAAAAATCAACAGAGGATAAAGAAAAAGAAACTAANCAATTATTAATTGAATCAG
AGAATAAATTAAATTTAACAATTGAATCGTTACATCAAGAATTTGATTCAAAGATATCAA
CCATTACAAATGAACATACAATTAATGAAAATAAAAATCATCAAGAAATCTTTTCATTAA
AAGATCAACTCTCAATTTTACAATCAAAAAATCAACAATCATTACAACAATTAGAGAAAA
TTCAAAAAGAAAATCAAGAGAAATCAATCTCTATTCAAAATGATAAATTATTAATTGAAA
AATTAGAATCTGATTTAAAAAATTCAAATCAAAATAATTTAGAAATTAATTCAACACTTG
ATTCAACTCAAGATACTTTAAAAAGTGTTTCAAAAGATTTATTCTTTTCAACTTGTTTAT
CAATTAAATTAAATTTATTAATGATTAGTAAACCATGTAATTTATCAACACAAGATCTTT
GGGAACAAGCAGAAGCATCACAAATTAGTTCATCAAATTATGCAAAATGGATATCTTTAA
AATTAAATGAATATCATAATGATTTACCACCTT

---IEEEKINLIXQLKELEDXQKSTEDKEKETXQLLIESENKLNLTIESLHQEFDSKIST
ITNEHTINENKNHQEIFSLKDQLSILQSKNQQSLQQLEKIQKENQEKSISIQNDKLLIEK
LESDLKNSNQNNLEINSTLDSTQDTLKSVSKDLFFSTCLSIKLNLLMISKPCNLSTQDLW
EQAEASQISSSNYAKWISLKLNEYHNDLPP

Frame A:
---IEEEKINLIXQLKELEDXQKSTEDKEKETXQLLIESENKLNLTIESLHQEFDSKIST
ITNEHTINENKNHQEIFSLKDQLSILQSKNQQSLQQLEKIQKENQEKSISIQNDKLLIEK
LESDLKNSNQNNLEINSTLDSTQDTLKSVSKDLFFSTCLSIKLNLLMISKPCNLSTQDLW
EQAEASQISSSNYAKWISLKLNEYHNDLPP


Frame B:
---lkkrkli*lxn*kn*kixknqqrikkkklxny*lnqrin*i*qlnryiknliqryqp
lqmniqlmkikiikksfh*kinsqfynqkinnhynn*rkfkkkikrnqslfkminy*lkn
*nli*kiqikii*kliqhliqlkil*kvfqkiysfqlvyqln*iy**lvnhviyqhkifg
nkqkhhklvhqimqngyl*n*mniimiyhl


Frame C:
---*rren*fn*xikrirr*xkinrg*rkrn*xiin*ire*ikfnn*ivtsri*fkdinh
yk*tyn**k*kssrnlfikrstlnftikkstiittirenskrksreinlysk**iin*ki
ri*fkkfksk*frn*fnt*fnsryfkkcfkrfilfnlfin*ikfind**tm*fintrslg
tsrsitn*fiklckmdifkik*is**ftt

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI165 (SFI165Q) /CSM/SF/SFI1-C/SFI165Q.Seq.d/                        472   e-132
SFD866 (SFD866Q) /CSM/SF/SFD8-C/SFD866Q.Seq.d/                        472   e-132
CFJ337 (CFJ337Q) /CSM/CF/CFJ3-B/CFJ337Q.Seq.d/                        472   e-132
SFJ893 (SFJ893Q) /CSM/SF/SFJ8-D/SFJ893Q.Seq.d/                        458   e-128
SFJ753 (SFJ753Q) /CSM/SF/SFJ7-C/SFJ753Q.Seq.d/                        440   e-122
SFJ495 (SFJ495Q) /CSM/SF/SFJ4-D/SFJ495Q.Seq.d/                        440   e-122
SFG432 (SFG432Q) /CSM/SF/SFG4-B/SFG432Q.Seq.d/                        440   e-122
SFD706 (SFD706Q) /CSM/SF/SFD7-A/SFD706Q.Seq.d/                        432   e-120
AFL636 (AFL636Q) /CSM/AF/AFL6-B/AFL636Q.Seq.d/                        428   e-119
VSE192 (VSE192Q) /CSM/VS/VSE1-D/VSE192Q.Seq.d/                         42   0.010

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   2e-11  23
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    40   1e-10  21
AL929352|AL929352.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 2/4.    34   2e-08  16
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    34   3e-08  19
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   4e-08  18
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    32   6e-08  18
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    34   7e-08  15
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           38   7e-08  24
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    34   8e-08  16
AC005504|AC005504.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 3 unordered pieces.    38   3e-07  13

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BA000016_1884(BA000016|pid:none) Clostridium perfringens str. 13...    47   3e-04
CP000312_1813(CP000312|pid:none) Clostridium perfringens SM101, ...    47   4e-04
T30010(T30010) hypothetical protein F58G4.1 - Caenorhabditis ele...    45   0.002
AL844509_339(AL844509|pid:none) Plasmodium falciparum 3D7 chromo...    43   0.006
AE014298_2867(AE014298|pid:none) Drosophila melanogaster chromos...    43   0.008
AB220160_1(AB220160|pid:none) Tetrahymena thermophila BNI1 mRNA ...    42   0.011
AC116957_105(AC116957|pid:none) Dictyostelium discoideum chromos...    42   0.011
AC117176_48(AC117176|pid:none) Dictyostelium discoideum chromoso...    42   0.014
AB366440_68(AB366440|pid:none) Salmonella enterica subsp. enteri...    42   0.018
(O76329) RecName: Full=Interaptin; AltName: Full=Alpha-actinin B...    41   0.024

 psg: 0.75  gvh: 0.24  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for SFI165 is nuc

>SFI165Z.Seq
NNNNNNNNNNAATTGAAGAAGAGAAAATTAATTTAATTAANCAATTAAAAGAATTAGAAG
ATAANCAAAAATCAACAGAGGATAAAGAAAAAGAAACTAANCAATTATTAATTGAATCAG
AGAATAAATTAAATTTAACAATTGAATCGTTACATCAAGAATTTGATTCAAAGATATCAA
CCATTACAAATGAACATACAATTAATGAAAATAAAAATCATCAAGAAATCTTTTCATTAA
AAGATCAACTCTCAATTTTACAATCAAAAAATCAACAATCATTACAACAATTAGAGAAAA
TTCAAAAAGAAAATCAAGAGAAATCAATCTCTATTCAAAATGATAAATTATTAATTGAAA
AATTAGAATCTGATTTAAAAAATTCAAATCAAAATAATTTAGAAATTAATTCAACACTTG
ATTCAACTCAAGATACTTTAAAAAGTGTTTCAAAAGATTTATTCTTTTCAACTTGTTTAT
CAATTAAATTAAATTTATTAATGATTAGTAAACCATGTAATTTATCAACACAAGATCTTT
GGGAACAAGCAGAAGCATCACAAATTAGTTCATCAAATTATGCAAAATGGATATCTTTAA
AATTAAATGAATATCATAATGATTTACCACCTT