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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFI162
Library SF
(Link to library)
Clone ID SFI162
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14954-1
Original site URL
Representative seq. ID SFI162P
(Link to Original site)
Representative DNA sequence 
>SFI162 (SFI162Q) /CSM/SF/SFI1-C/SFI162Q.Seq.d/
ACTGTTGGCCTACTGGAAAAAATGATTAAAAAAGTGCATTAATAACATTATTTTTAGTTT
CACTAATTTTGGGTGTTTCTTTATCCCAAAAACCATTTTATTGCCAAGCACCAGAACCAA
CACCAAGTTTAAATACAGATGGATTAACATTAAAGATGGTACAAATTTTAACAAGACATG
GTGATAGAACACCATTGTATAGTACATTAAAACCAACAATGAATACATGGGATTGTAATT
TAGGATGGTTAATGGTATCAAGTTTAAATAATGTACCAGGAGCAGCAACAGATGTTGATA
GATTATTTAGAAAAGTTTATATGCCAAATAGAGAATATTTCCCAGGTAATTGTTCAGACG
GTCAATTGACCTCACTTGGATTCCAACAACATTTACAATTAGGTCAAAGTTTACGTCAAC
TTCTACGTTGATAAATATGAATTATTACCAAGTGAATTATCAGTGGATGCAGCATCAACC
AXXXXXXXXXXCGTCTTGGNANGTNTACNTTNTTAAAGGAGGNAGTCGATAANATTANAG
CTTTTGTTAATGGGACTTCCTCTGNCAAANAAATCGTTTTCTCNGGNCANGATGANTCAG
NTGGNCCNTTCNCTAATTTATTTGGTNTCANGAAANAANGGCCCCCNTANGCTTCNCATG
TTGAACNT
sequence update 2001.11.22
Translated Amino Acid sequence 
llaywkk*lkkcinniifsftnfgcffipktillpstrtntkfkyrwinikdgtnfnktw
**ntiv*yiktnneymgl*frmvngikfk*ctrssnrc**ii*kslyak*rifpr*LFRR
SIDLTWIPTTFTIRSKFTSTSTLINMNYYQVNYQWMQHQP---

---RLGXXTXLKEXVDXIXAFVNGTSSXKXIVFSGXDXSXGPFXNLFGXXKXXPPXASHV
EX
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllekmikkvh**hyf*fh*fwvflypknhfiakhqnqhqv*iqmd*h*rwykf*qdm
viehhcivh*nqq*ihgivi*dg*wyqv*imyqeqqqmlidylekficqienisqvivqt
vn*phldsnniyn*vkvyvnfyvdkyellpselsvdaast---

---RLGXXTXLKEXVDXIXAFVNGTSSXKXIVFSGXDXSXGPFXNLFGXXKXXPPXASHV
EX

Frame B:
llaywkk*lkkcinniifsftnfgcffipktillpstrtntkfkyrwinikdgtnfnktw
**ntiv*yiktnneymgl*frmvngikfk*ctrssnrc**ii*kslyak*rifpr*LFRR
SIDLTWIPTTFTIRSKFTSTSTLINMNYYQVNYQWMQHQP---

---vlxxxxx*rrxsixlxlllmglplxxksfsxxxmxqxxxsliylvsxxxgpxxlxml
n

Frame C:
cwptgknd*ksalitlflvslilgvslsqkpfycqapeptpslntdgltlkmvqiltrhg
drtplystlkptmntwdcnlgwlmvsslnnvpgaatdvdrlfrkvympnreyfpgncsdg
qltslgfqqhlqlgqslrqllr**i*iitk*iisgcsin---

---swxvyxxkggsr*xxsfc*wdflxqxnrflxxx*xxwxxx*fiwxxexxapxxfxc*
t
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI162 (SFI162Q) /CSM/SF/SFI1-C/SFI162Q.Seq.d/                       1112   0.0
CFA408 (CFA408Q) /CSM/CF/CFA4-A/CFA408Q.Seq.d/                        896   0.0
CHD582 (CHD582Q) /CSM/CH/CHD5-D/CHD582Q.Seq.d/                        894   0.0
CFB709 (CFB709Q) /CSM/CF/CFB7-A/CFB709Q.Seq.d/                        894   0.0
AFD586 (AFD586Q) /CSM/AF/AFD5-D/AFD586Q.Seq.d/                        894   0.0
VFG714 (VFG714Q) /CSM/VF/VFG7-A/VFG714Q.Seq.d/                        892   0.0
VFM877 (VFM877Q) /CSM/VF/VFM8-D/VFM877Q.Seq.d/                        890   0.0
VFM810 (VFM810Q) /CSM/VF/VFM8-A/VFM810Q.Seq.d/                        890   0.0
VFL178 (VFL178Q) /CSM/VF/VFL1-D/VFL178Q.Seq.d/                        890   0.0
VFG445 (VFG445Q) /CSM/VF/VFG4-B/VFG445Q.Seq.d/                        890   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AP006628|AP006628.1 Onion yellows phytoplasma DNA, complete genome.    34   0.049  14
U13645|U13645.1 Caenorhabditis elegans cosmid C05D10, complete sequence.    32   0.26   5
BH164673|BH164673.1 ENTTE80TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38   0.39   2
AZ544963|AZ544963.1 ENTEB40TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38   0.39   2
AE014832|AE014832.1 Plasmodium falciparum 3D7 chromosome 10 section 4 of 7 of the complete sequence.    36   0.43   9
BH710149|BH710149.1 BOMIE65TR BO_2_3_KB Brassica oleracea genomic clone BOMIE65, DNA sequence.    32   0.43   2
AP002953|AP002953.2 Oryza sativa genomic DNA, chromosome 1, PAC clone:P0426D06.    46   0.61   1
CF594621|CF594621.1 AGENCOURT_15713293 NIH_ZGC_8 Danio rerio cDNA clone IMAGE:7014522 5', mRNA sequence.    46   0.61   1
AC116925|AC116925.2 Dictyostelium discoideum chromosome 2 map 4189423-4228998 strain AX4, complete sequence.    36   0.90   4
BX293567|BX293567.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEYP-7A3.    38   0.98   6
dna update 2003.12.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54PR9) RecName: Full=Counting factor 60; Flags: Precursor;  &D...   246   4e-66
(P24031) RecName: Full=Constitutive acid phosphatase;          E...    35   2.5
X01080_2(X01080|pid:none) Yeast genes PH05 and PH03 for repressi...    35   2.5
(Q6DH46) RecName: Full=Acid phosphatase-like protein 2;         ...    35   2.5
AF293415_1(AF293415|pid:none) Giardia intestinalis acid phosphat...    34   4.3
D88504(D88504)protein B0361.7 [imported] - Caenorhabditis elegans      33   5.7
(Q10944) RecName: Full=Putative acid phosphatase B0361.7;       ...    33   5.7
BC125717_1(BC125717|pid:none) Xenopus tropicalis hypothetical pr...    33   9.7
protein update 2009. 1.11
PSORT 

 psg: 0.91  gvh: 0.46  alm: 0.49  top: 0.47  tms: 0.00  mit: 0.57  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: vacuolar

>> prediction for SFI162 is mit
5' end seq. ID SFI162F
5' end seq. 
>SFI162F.Seq
ACTGTTGGCCTACTGGAAAAAATGATTAAAAAAGTGCATTAATAACATTATTTTTAGTTT
CACTAATTTTGGGTGTTTCTTTATCCCAAAAACCATTTTATTGCCAAGCACCAGAACCAA
CACCAAGTTTAAATACAGATGGATTAACATTAAAGATGGTACAAATTTTAACAAGACATG
GTGATAGAACACCATTGTATAGTACATTAAAACCAACAATGAATACATGGGATTGTAATT
TAGGATGGTTAATGGTATCAAGTTTAAATAATGTACCAGGAGCAGCAACAGATGTTGATA
GATTATTTAGAAAAGTTTATATGCCAAATAGAGAATATTTCCCAGGTAATTGTTCAGACG
GTCAATTGACCTCACTTGGATTCCAACAACATTTACAATTAGGTCAAAGTTTACGTCAAC
TTCTACGTTGATAAATATGAATTATTACCAAGTGAATTATCAGTGGATGCAGCATCAACC
A----------
Length of 5' end seq. 481
3' end seq. ID SFI162Z
3' end seq. 
>SFI162Z.Seq
NNNNNNNNNNCGTCTTGGNANGTNTACNTTNTTAAAGGAGGNAGTCGATAANATTANAGC
TTTTGTTAATGGGACTTCCTCTGNCAAANAAATCGTTTTCTCNGGNCANGATGANTCAGN
TGGNCCNTTCNCTAATTTATTTGGTNTCANGAAANAANGGCCCCCNTANGCTTCNCATGT
TGAACNT
Length of 3' end seq. 187
Connected seq. ID SFI162P
Connected seq. 
>SFI162P.Seq
ACTGTTGGCCTACTGGAAAAAATGATTAAAAAAGTGCATTAATAACATTATTTTTAGTTT
CACTAATTTTGGGTGTTTCTTTATCCCAAAAACCATTTTATTGCCAAGCACCAGAACCAA
CACCAAGTTTAAATACAGATGGATTAACATTAAAGATGGTACAAATTTTAACAAGACATG
GTGATAGAACACCATTGTATAGTACATTAAAACCAACAATGAATACATGGGATTGTAATT
TAGGATGGTTAATGGTATCAAGTTTAAATAATGTACCAGGAGCAGCAACAGATGTTGATA
GATTATTTAGAAAAGTTTATATGCCAAATAGAGAATATTTCCCAGGTAATTGTTCAGACG
GTCAATTGACCTCACTTGGATTCCAACAACATTTACAATTAGGTCAAAGTTTACGTCAAC
TTCTACGTTGATAAATATGAATTATTACCAAGTGAATTATCAGTGGATGCAGCATCAACC
A----------CGTCTTGGNANGTNTACNTTNTTAAAGGAGGNAGTCGATAANATTANAG
CTTTTGTTAATGGGACTTCCTCTGNCAAANAAATCGTTTTCTCNGGNCANGATGANTCAG
NTGGNCCNTTCNCTAATTTATTTGGTNTCANGAAANAANGGCCCCCNTANGCTTCNCATG
TTGAACNT
Length of connected seq. 658
Full length Seq ID -
Full length Seq. -
Length of full length seq. -