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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFI140
Library SF
(Link to library)
Clone ID SFI140
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16358-1
Original site URL
Representative seq. ID SFI140P
(Link to Original site)
Representative DNA sequence 
>SFI140 (SFI140Q) /CSM/SF/SFI1-B/SFI140Q.Seq.d/
ACTGTTGGCCTACTGGACAAAAAACATTTTCTAATAAAACAGATAAATTAAAAAATGACT
ACCTTTAACGAATATCCATTCTTGGCTGAATTAGGCATTAAAGCTGAAAATAATGATGGA
GTCTTCAATGGAAAATGGGGAGGTGCTGGTGAAATCATCAAATGTTTAAATCCAACAAAT
AATAAAGTTATTGCAACTGTTAGAGGTGCTGCACCAGAAGAATATGAAACATGTATTCAA
GCAATGTTAGCAGCAAAAGTAAAATGGGCATTAACACCAXXXXXXXXXXTGAAGGTNTTN
AANAAATAAAAAANCAAGGTGGTAAGGTCGTCATTGGTGGTAATAAACTCGANATCAGTG
GTGGAAACTTTGTTNAACCAACCGTCGTTGCCATTGAACATGATGCTCCNATNGTAAANA
CTGAACTNTTTGTTCCAATTNTNTACATAATGAAATTCAAAAATNTTGACGATGCTTTTG
CCTGGAATAATGAAGTCCCNCAAGGTNTNTCATNTTCCCTNTTCNCAAACAATCAAAANA
ATATCTTCAAATGGCTTGGACCNACCGGATCTGACTGTGGNATAGTTAACGTAAATGNAG
CTACNAACGGNGCTGAAATTGGTGGNGCCNTTGGAGGTGAAAAAAAGACCGGAGGTGGTA
AANAATCTGGCNCAGACAGCTGGAAACAGTATTGCCGTCGT
sequence update 2001.11.22
Translated Amino Acid sequence 
tvglldkkhflikqin*KMTTFNEYPFLAELGIKAENNDGVFNGKWGGAGEIIKCLNPTN
NKVIATVRGAAPEEYETCIQAMLAAKVKWALTP---

---EGXXXIKXQGGKVVIGGNKLXISGGNFVXPTVVAIEHDAPXVXTELFVPIXYIMKFK
NXDDAFAWNNEVPQGXSXSLFXNNQXNIFKWLGPTGSDCGIVNVNXATNGAEIGGAXGGE
KKTGGGKXSGXDSWKQYCRR
Translated Amino Acid sequence (All Frames) 
Frame A:
tvglldkkhflikqin*KMTTFNEYPFLAELGIKAENNDGVFNGKWGGAGEIIKCLNPTN
NKVIATVRGAAPEEYETCIQAMLAAKVKWALTP---

---*rxxxnkkxrw*grhww**trxqwwklcxtnrrch*t*csxxkx*txcsnxxhneiq
kx*rcfcle**spxrxxxfpxxkqskxylqmawtxri*lwxs*rkxsyxrx*nwwxxwr*
kkdrrw*xiwxrqletvlps

Frame B:
llaywtknif**nr*ikk*lpltnihswln*alklkimmessmengevlvkssnv*iqqi
ikllqllevlhqknmkhvfkqc*qqk*ngh*h---

---EGXXXIKXQGGKVVIGGNKLXISGGNFVXPTVVAIEHDAPXVXTELFVPIXYIMKFK
NXDDAFAWNNEVPQGXSXSLFXNNQXNIFKWLGPTGSDCGIVNVNXATNGAEIGGAXGGE
KKTGGGKXSGXDSWKQYCRR

Frame C:
cwptgqktfsnktdklkndyl*risilg*irh*s*k**wslqwkmgrcw*nhqmfksnk*
*sycnc*rcctrri*nmyssnvsskskmgint---

---kvxxk*kxkvvrsslvvinsxsvvetlxnqpslplnmmlxx*xlnxlfqxxt**nsk
xltmllpgimksxkvxhxpxsqtikxissngldxpdltvx*lt*mxlxtxlklvxplevk
krpevvxnlaqtagnsiav
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI140 (SFI140Q) /CSM/SF/SFI1-B/SFI140Q.Seq.d/                       1080   0.0
VFI491 (VFI491Q) /CSM/VF/VFI4-D/VFI491Q.Seq.d/                        577   e-164
CFJ439 (CFJ439Q) /CSM/CF/CFJ4-B/CFJ439Q.Seq.d/                        577   e-164
VFL108 (VFL108Q) /CSM/VF/VFL1-A/VFL108Q.Seq.d/                        571   e-162
VFI240 (VFI240Q) /CSM/VF/VFI2-B/VFI240Q.Seq.d/                        571   e-162
SFI194 (SFI194Q) /CSM/SF/SFI1-D/SFI194Q.Seq.d/                        571   e-162
SFB124 (SFB124Q) /CSM/SF/SFB1-A/SFB124Q.Seq.d/                        571   e-162
CFK204 (CFK204Q) /CSM/CF/CFK2-A/CFK204Q.Seq.d/                        571   e-162
CFC586 (CFC586Q) /CSM/CF/CFC5-D/CFC586Q.Seq.d/                        571   e-162
VFJ233 (VFJ233Q) /CSM/VF/VFJ2-B/VFJ233Q.Seq.d/                        569   e-161
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.   507   0.0    6
BU796678|BU796678.1 SJF2EEH06 SJF Schistosoma japonicum cDNA, mRNA sequence.    48   0.16   1
BU111535|BU111535.1 603126401F1 CSEQCHL13 Gallus gallus cDNA clone ChEST96a2 5', mRNA sequence.    42   0.27   2
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    36   0.27   8
CD733534|CD733534.1 4045967 1GAL - Chicken Intestinal Lymphocyte Gallus gallus cDNA clone 1GAL_17H10 5', mRNA sequence.    42   0.28   2
BU399611|BU399611.1 603481881F1 CSEQCHN59 Gallus gallus cDNA clone ChEST370a22 5', mRNA sequence.    42   0.28   2
BG711333| (S74728) antiquitin=26g turgor protein homolog [human, kidney, Peptide, 511 aa] [Homo sapiens]G, mRNA sequence.    42   0.29   2
BU358377|BU358377.1 603476320F1 CSEQCHN71 Gallus gallus cDNA clone ChEST360h6 5', mRNA sequence.    42   0.29   2
BU488446|BU488446.1 604125245F1 CSEQRBN36 Gallus gallus cDNA clone ChEST943i12 5', mRNA sequence.    42   0.31   2
BU319046|BU319046.1 603486759F1 CSEQCHN62 Gallus gallus cDNA clone ChEST382f16 5', mRNA sequence.    42   0.31   2
dna update 2003.12.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P83401) RecName: Full=Putative aldehyde dehydrogenase family 7 ...   171   2e-41
AY847462_1(AY847462|pid:none) Acanthopagrus schlegelii antiquiti...   119   9e-26
BT044648_1(BT044648|pid:none) Salmo salar clone ssal-rgf-001-326...   118   1e-25
AY250704_1(AY250704|pid:none) Glycine max aldehyde dehydrogenase...   115   1e-24
BC044367_1(BC044367|pid:none) Danio rerio aldehyde dehydrogenase...   114   4e-24
AK297365_1(AK297365|pid:none) Homo sapiens cDNA FLJ56008 complet...   112   1e-23
(P49419) RecName: Full=Alpha-aminoadipic semialdehyde dehydrogen...   112   1e-23
EF215151_1(EF215151|pid:none) Callithrix jacchus clone G21-51.1_...   112   1e-23
(Q41247) RecName: Full=Aldehyde dehydrogenase family 7 member A1...   110   3e-23
AY883858_1(AY883858|pid:none) Sterkiella histriomuscorum turgor ...   110   3e-23
protein update 2009. 5.25
PSORT 

 psg: 0.78  gvh: 0.31  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SFI140 is cyt
5' end seq. ID SFI140F
5' end seq. 
>SFI140F.Seq
ACTGTTGGCCTACTGGACAAAAAACATTTTCTAATAAAACAGATAAATTAAAAAATGACT
ACCTTTAACGAATATCCATTCTTGGCTGAATTAGGCATTAAAGCTGAAAATAATGATGGA
GTCTTCAATGGAAAATGGGGAGGTGCTGGTGAAATCATCAAATGTTTAAATCCAACAAAT
AATAAAGTTATTGCAACTGTTAGAGGTGCTGCACCAGAAGAATATGAAACATGTATTCAA
GCAATGTTAGCAGCAAAAGTAAAATGGGCATTAACACCA----------
Length of 5' end seq. 279
3' end seq. ID SFI140Z
3' end seq. 
>SFI140Z.Seq
NNNNNNNNNNTGAAGGTNTTNAANAAATAAAAAANCAAGGTGGTAAGGTCGTCATTGGTG
GTAATAAACTCGANATCAGTGGTGGAAACTTTGTTNAACCAACCGTCGTTGCCATTGAAC
ATGATGCTCCNATNGTAAANACTGAACTNTTTGTTCCAATTNTNTACATAATGAAATTCA
AAAATNTTGACGATGCTTTTGCCTGGAATAATGAAGTCCCNCAAGGTNTNTCATNTTCCC
TNTTCNCAAACAATCAAAANAATATCTTCAAATGGCTTGGACCNACCGGATCTGACTGTG
GNATAGTTAACGTAAATGNAGCTACNAACGGNGCTGAAATTGGTGGNGCCNTTGGAGGTG
AAAAAAAGACCGGAGGTGGTAAANAATCTGGCNCAGACAGCTGGAAACAGTATTGCCGTC
GT
Length of 3' end seq. 422
Connected seq. ID SFI140P
Connected seq. 
>SFI140P.Seq
ACTGTTGGCCTACTGGACAAAAAACATTTTCTAATAAAACAGATAAATTAAAAAATGACT
ACCTTTAACGAATATCCATTCTTGGCTGAATTAGGCATTAAAGCTGAAAATAATGATGGA
GTCTTCAATGGAAAATGGGGAGGTGCTGGTGAAATCATCAAATGTTTAAATCCAACAAAT
AATAAAGTTATTGCAACTGTTAGAGGTGCTGCACCAGAAGAATATGAAACATGTATTCAA
GCAATGTTAGCAGCAAAAGTAAAATGGGCATTAACACCA----------TGAAGGTNTTN
AANAAATAAAAAANCAAGGTGGTAAGGTCGTCATTGGTGGTAATAAACTCGANATCAGTG
GTGGAAACTTTGTTNAACCAACCGTCGTTGCCATTGAACATGATGCTCCNATNGTAAANA
CTGAACTNTTTGTTCCAATTNTNTACATAATGAAATTCAAAAATNTTGACGATGCTTTTG
CCTGGAATAATGAAGTCCCNCAAGGTNTNTCATNTTCCCTNTTCNCAAACAATCAAAANA
ATATCTTCAAATGGCTTGGACCNACCGGATCTGACTGTGGNATAGTTAACGTAAATGNAG
CTACNAACGGNGCTGAAATTGGTGGNGCCNTTGGAGGTGAAAAAAAGACCGGAGGTGGTA
AANAATCTGGCNCAGACAGCTGGAAACAGTATTGCCGTCGT
Length of connected seq. 691
Full length Seq ID -
Full length Seq. -
Length of full length seq. -