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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFI129
Library SF
(Link to library)
Clone ID SFI129
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SFI129P
(Link to Original site)
Representative DNA sequence 
>SFI129 (SFI129Q) /CSM/SF/SFI1-B/SFI129Q.Seq.d/
ACTGTTGGCCTACTGGNAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAA
GAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATT
TTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGG
TTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTA
AATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACG
CTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACA
CTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCA
AATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATG
TTTGXXXXXXXXXXCAAAGAGATCCAAGCAGGTANCCTCGCTGATGATTGGGAATTACTT
CCATCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAG
GAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATC
GTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAA
GCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAAT
CCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGAC
AATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAA
TCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTC
TCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAA
GCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCT
GAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTT
AAAACTGAGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCAGACGAACTT
TAAANAAATAAATAAATA
sequence update 2001.11.22
Translated Amino Acid sequence 
cwptgnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWV
VSDWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYT
VKNEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDV---

---KEIQAGXLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVD
PEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNE
LYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAA
KAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTETKKKKSKNQPKKXSDEL*x
nk*i
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdg
llvigikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsnt
l*rmnkklivvvhtssyyhpn*inqhsmvnlniplclvqmf---

---qrdpsrxpr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*
srscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*
tlpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsr
qs*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n*dkkeevkkstkkvirrtlx
k*in

Frame B:
llaywxssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmg
c**la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspih
cke*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrcl---

---KEIQAGXLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVD
PEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNE
LYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAA
KAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTETKKKKSKNQPKKXSDEL*x
nk*i

Frame C:
cwptgnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWV
VSDWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYT
VKNEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDV---

---krskqvxslmignyfhqnkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsli
qklsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmn
stslmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskpp
klkrerkltkn*lpkkllkkkpkklmkkkkkslkki*lklrqkrrsqkinqksxqtnfkx
ink
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI129 (SFI129Q) /CSM/SF/SFI1-B/SFI129Q.Seq.d/                       1725   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                       1047   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                       1047   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       1047   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                       1047   0.0
SFH171 (SFH171Q) /CSM/SF/SFH1-C/SFH171Q.Seq.d/                       1047   0.0
SFG873 (SFG873Q) /CSM/SF/SFG8-D/SFG873Q.Seq.d/                       1047   0.0
SFA732 (SFA732Q) /CSM/SF/SFA7-B/SFA732Q.Seq.d/                       1047   0.0
CHC137 (CHC137Q) /CSM/CH/CHC1-B/CHC137Q.Seq.d/                       1047   0.0
AFO794 (AFO794Q) /CSM/AF/AFO7-D/AFO794Q.Seq.d/                       1047   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1047   0.0    3
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   2e-24  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   3e-20  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   8e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   2e-10  2
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74   5e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74   5e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74   5e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74   5e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   5e-09  1
dna update 2003.12.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   326   9e-88
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   205   2e-51
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   199   1e-49
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   197   8e-49
AK230218_1(AK230218|pid:none) Arabidopsis thaliana mRNA for hypo...   196   1e-48
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   195   3e-48
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   195   3e-48
AC149080_5(AC149080|pid:none) Medicago truncatula clone mth2-75j...   193   9e-48
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   193   9e-48
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   193   1e-47
protein update 2009. 5.25
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for SFI129 is exc
5' end seq. ID SFI129F
5' end seq. 
>SFI129F.Seq
ACTGTTGGCCTACTGGNAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAA
GAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATT
TTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGG
TTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTA
AATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACG
CTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACA
CTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCA
AATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATG
TTTG----------
Length of 5' end seq. 484
3' end seq. ID SFI129Z
3' end seq. 
>SFI129Z.Seq
NNNNNNNNNNCAAAGAGATCCAAGCAGGTANCCTCGCTGATGATTGGGAATTACTTCCAT
CAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAA
TCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTG
ATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCC
CAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAG
AATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATG
AACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTG
GTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTG
AAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCG
CCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAA
AAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAA
CTGAGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCAGACGAACTTTAAA
NAAATAAATAAATA
Length of 3' end seq. 734
Connected seq. ID SFI129P
Connected seq. 
>SFI129P.Seq
ACTGTTGGCCTACTGGNAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAA
GAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATT
TTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGG
TTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTA
AATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACG
CTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACA
CTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCA
AATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATG
TTTG----------CAAAGAGATCCAAGCAGGTANCCTCGCTGATGATTGGGAATTACTT
CCATCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAG
GAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATC
GTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAA
GCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAAT
CCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGAC
AATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAA
TCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTC
TCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAA
GCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCT
GAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTT
AAAACTGAGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCAGACGAACTT
TAAANAAATAAATAAATA
Length of connected seq. 1208
Full length Seq ID -
Full length Seq. -
Length of full length seq. -