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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFH864
Library SF
(Link to library)
Clone ID SFH864
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16451-1
Original site URL
Representative seq. ID SFH864P
(Link to Original site)
Representative DNA sequence 
>SFH864 (SFH864Q) /CSM/SF/SFH8-C/SFH864Q.Seq.d/
ACTGTTGGCCTACTGGNAACTCGACCTAACAAATAAAAAAAGCAAAATGCAAAATAAACC
ATCATTCATTTTAAGAAACCCATCAGCTAATAAAGGTACTGGTTTTAATAATGAAGAAAG
AGAAAAATTAGGATTAAAAGGTTTATTACCACCAAAGGTTGAATCATTACAAGAACAATC
AGATAGAGCATTATCACAATTTACATCATTTAATACAAACTTGGAAAGATATATTTTCTT
AAATTGTTTACGTGATCGTAATGAAACTTTATTCTATTATTTATTAAGTAATAATTTAGA
ATTAATGATGCCAATCATTTATACACCAACCGTAGGTGAAGCATGTCAAAAGTTTGGTAA
TGAATTCCGTTTCGCCCAAGGTATGTATTTTGCATCCCAAGATAAAGGTAACATTCGTGC
CATGATGGACAATTGGCCAGCCGAAGGTGTCGATATTATCGTTGTTTCCGATGGTTCACG
TATTTTGGGTTTAGGTGATCTCGGTACCAACGGTATGGGTATCCCAGTTGGTAAGTTACA
ATTGTACGTCGCCGGTGCTGGTTTCTGTCCAAXXXXXXXXXXCCATNCAAGGNCAGTNGA
ATACAAAGGTAAAACCTTTGTTNCAGGTCAAGGTANCAATATGTACATCTTCCCAGGTCT
TGGTTTAGCCGCTAGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGATCATTACTGC
CGCTAAAACTCTTGCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTAAAATCTATCC
AGGTCTCCAACATATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCATTGAAAAAGC
TTATGAAGAAGGTATGGCTCAATTACCACGTCCAGATAATATTGAAGCTTTAGTCAAATC
TCGTCAATATGTTCCATACTTATGATAAATCAAAAAATTGACTATNCTTGCCCAATTTC
sequence update 2001.11.22
Translated Amino Acid sequence 
LLAYWXLDLTNKKSKMQNKPSFILRNPSANKGTGFNNEEREKLGLKGLLPPKVESLQEQS
DRALSQFTSFNTNLERYIFLNCLRDRNETLFYYLLSNNLELMMPIIYTPTVGEACQKFGN
EFRFAQGMYFASQDKGNIRAMMDNWPAEGVDIIVVSDGSRILGLGDLGTNGMGIPVGKLQ
LYVAGAGFCP---

---HXRXVEYKGKTFVXGQGXNMYIFPGLGLAASVCEAKHVTDAMIITAAKTLASFVEDS
EVLTGKIYPGLQHIREISTRIAVKVIEKAYEEGMAQLPRPDNIEALVKSRQYVPYL**ik
kltxlaqf
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxtrpnk*kkqnak*tiihfkkpis**rywf***rkrkirikrfittkg*iitrti
r*siitiyii*yklgkiyflklft*s**nfillfik**frindanhlytnrr*smskvw*
*ipfrpryvfcipr*r*hschdgqlasrrcryyrcfrwftyfgfr*sryqrygypsw*vt
ivrrrcwfls---

---pxkxsxiqr*nlcxrsrxqyvhlprswfsr*rl*s*tch*cydhycr*nscllcrrl
*sshw*nlsrsptys*nlnknrr*sh*ksl*rrygsittsr*y*sfsqissicsilminq
kidyxcpi

Frame B:
LLAYWXLDLTNKKSKMQNKPSFILRNPSANKGTGFNNEEREKLGLKGLLPPKVESLQEQS
DRALSQFTSFNTNLERYIFLNCLRDRNETLFYYLLSNNLELMMPIIYTPTVGEACQKFGN
EFRFAQGMYFASQDKGNIRAMMDNWPAEGVDIIVVSDGSRILGLGDLGTNGMGIPVGKLQ
LYVAGAGFCP---

---HXRXVEYKGKTFVXGQGXNMYIFPGLGLAASVCEAKHVTDAMIITAAKTLASFVEDS
EVLTGKIYPGLQHIREISTRIAVKVIEKAYEEGMAQLPRPDNIEALVKSRQYVPYL**ik
kltxlaqf

Frame C:
cwptgnst*qikkakckinhhsf*ethqlikvlvlimkkekn*d*kvyyhqrlnhyknnq
iehyhnlhhliqtwkdifs*ivyvivmklysiiy*vii*n**cqsfihqp*vkhvkslvm
nsvspkvcilhpkikvtfvp*wtigqpkvsilslfpmvhvfwv*visvptvwvsqlvsyn
ctspvlvsvq---

---xqgqxntkvkplxqvkvxictssqvlv*plafvklnmslml*sllplkllppl*ktl
kfslvksiqvsnifvksqqesplkslkklmkkvwlnyhvqiilkl*snlvnmfhtydksk
n*lxlpnf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH864 (SFH864Q) /CSM/SF/SFH8-C/SFH864Q.Seq.d/                       1758   0.0
VFI106 (VFI106Q) /CSM/VF/VFI1-A/VFI106Q.Seq.d/                       1059   0.0
CFJ334 (CFJ334Q) /CSM/CF/CFJ3-B/CFJ334Q.Seq.d/                       1059   0.0
CFG469 (CFG469Q) /CSM/CF/CFG4-C/CFG469Q.Seq.d/                       1059   0.0
VFK276 (VFK276Q) /CSM/VF/VFK2-D/VFK276Q.Seq.d/                       1045   0.0
CFH782 (CFH782Q) /CSM/CF/CFH7-D/CFH782Q.Seq.d/                       1045   0.0
VFI253 (VFI253Q) /CSM/VF/VFI2-C/VFI253Q.Seq.d/                       1041   0.0
VFJ368 (VFJ368Q) /CSM/VF/VFJ3-C/VFJ368Q.Seq.d/                       1023   0.0
CHA831 (CHA831Q) /CSM/CH/CHA8-B/CHA831Q.Seq.d/                       1019   0.0
CHB626 (CHB626Q) /CSM/CH/CHB6-B/CHB626Q.Seq.d/                        809   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY387644|AY387644.1 Dictyostelium discoideum malic enzyme gene, complete cds.   950   0.0    6
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   942   0.0    8
AE017029|AE017029.1 Bacillus anthracis str. Ames section 6 of 18 of the complete genome.    52   0.014  1
Y09212|Y09212.1 B.cereus partial gltT, aspA and partial maoX genes.    52   0.014  1
AE014846|AE014846.1 Plasmodium falciparum 3D7 chromosome 12, section 3 of 9 of the complete sequence.    34   0.028  11
AY194854|AY194854.1 Meloidogyne mayaguensis 5S-18S ribosomal RNA intergenic spacer, partial sequence.    40   0.028  2
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.           36   0.044  22
AF177535|AF177535.1 Arabidopsis thaliana BAC F26C17.               40   0.081  5
AP003187|AP003187.2 Clostridium perfringens DNA, complete genome, section 3/10.    36   0.093  10
BM026948|BM026948.1 GIT0000207 Root-induced cDNA library from Glomus intraradices Glomus intraradices cDNA, mRNA sequence.    32   0.19   3
dna update 2003.12.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001322_605(CP001322|pid:none) Desulfatibacillum alkenivorans A...   179   2e-43
AY149910_1(AY149910|pid:none) Mastigamoeba balamuthi malic enzym...   171   3e-41
DQ901690_1(DQ901690|pid:none) Tigriopus californicus haplotype S...   171   4e-41
DQ901654_1(DQ901654|pid:none) Tigriopus californicus haplotype A...   171   4e-41
DQ901663_1(DQ901663|pid:none) Tigriopus californicus haplotype A...   171   4e-41
DQ901691_1(DQ901691|pid:none) Tigriopus californicus haplotype S...   171   4e-41
DQ901669_1(DQ901669|pid:none) Tigriopus californicus haplotype S...   171   4e-41
DQ901657_1(DQ901657|pid:none) Tigriopus californicus haplotype A...   171   4e-41
DQ901666_1(DQ901666|pid:none) Tigriopus californicus haplotype S...   171   4e-41
CP000581_12(CP000581|pid:none) Ostreococcus lucimarinus CCE9901 ...   169   2e-40
protein update 2009. 5.25
PSORT 

 psg: 0.75  gvh: 0.55  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for SFH864 is cyt
5' end seq. ID SFH864F
5' end seq. 
>SFH864F.Seq
ACTGTTGGCCTACTGGNAACTCGACCTAACAAATAAAAAAAGCAAAATGCAAAATAAACC
ATCATTCATTTTAAGAAACCCATCAGCTAATAAAGGTACTGGTTTTAATAATGAAGAAAG
AGAAAAATTAGGATTAAAAGGTTTATTACCACCAAAGGTTGAATCATTACAAGAACAATC
AGATAGAGCATTATCACAATTTACATCATTTAATACAAACTTGGAAAGATATATTTTCTT
AAATTGTTTACGTGATCGTAATGAAACTTTATTCTATTATTTATTAAGTAATAATTTAGA
ATTAATGATGCCAATCATTTATACACCAACCGTAGGTGAAGCATGTCAAAAGTTTGGTAA
TGAATTCCGTTTCGCCCAAGGTATGTATTTTGCATCCCAAGATAAAGGTAACATTCGTGC
CATGATGGACAATTGGCCAGCCGAAGGTGTCGATATTATCGTTGTTTCCGATGGTTCACG
TATTTTGGGTTTAGGTGATCTCGGTACCAACGGTATGGGTATCCCAGTTGGTAAGTTACA
ATTGTACGTCGCCGGTGCTGGTTTCTGTCCAA----------
Length of 5' end seq. 572
3' end seq. ID SFH864Z
3' end seq. 
>SFH864Z.Seq
NNNNNNNNNNCCATNCAAGGNCAGTNGAATACAAAGGTAAAACCTTTGTTNCAGGTCAAG
GTANCAATATGTACATCTTCCCAGGTCTTGGTTTAGCCGCTAGCGTTTGTGAAGCTAAAC
ATGTCACTGATGCTATGATCATTACTGCCGCTAAAACTCTTGCCTCCTTTGTAGAAGACT
CTGAAGTTCTCACTGGTAAAATCTATCCAGGTCTCCAACATATTCGTGAAATCTCAACAA
GAATCGCCGTTAAAGTCATTGAAAAAGCTTATGAAGAAGGTATGGCTCAATTACCACGTC
CAGATAATATTGAAGCTTTAGTCAAATCTCGTCAATATGTTCCATACTTATGATAAATCA
AAAAATTGACTATNCTTGCCCAATTTC
Length of 3' end seq. 387
Connected seq. ID SFH864P
Connected seq. 
>SFH864P.Seq
ACTGTTGGCCTACTGGNAACTCGACCTAACAAATAAAAAAAGCAAAATGCAAAATAAACC
ATCATTCATTTTAAGAAACCCATCAGCTAATAAAGGTACTGGTTTTAATAATGAAGAAAG
AGAAAAATTAGGATTAAAAGGTTTATTACCACCAAAGGTTGAATCATTACAAGAACAATC
AGATAGAGCATTATCACAATTTACATCATTTAATACAAACTTGGAAAGATATATTTTCTT
AAATTGTTTACGTGATCGTAATGAAACTTTATTCTATTATTTATTAAGTAATAATTTAGA
ATTAATGATGCCAATCATTTATACACCAACCGTAGGTGAAGCATGTCAAAAGTTTGGTAA
TGAATTCCGTTTCGCCCAAGGTATGTATTTTGCATCCCAAGATAAAGGTAACATTCGTGC
CATGATGGACAATTGGCCAGCCGAAGGTGTCGATATTATCGTTGTTTCCGATGGTTCACG
TATTTTGGGTTTAGGTGATCTCGGTACCAACGGTATGGGTATCCCAGTTGGTAAGTTACA
ATTGTACGTCGCCGGTGCTGGTTTCTGTCCAA----------CCATNCAAGGNCAGTNGA
ATACAAAGGTAAAACCTTTGTTNCAGGTCAAGGTANCAATATGTACATCTTCCCAGGTCT
TGGTTTAGCCGCTAGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGATCATTACTGC
CGCTAAAACTCTTGCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTAAAATCTATCC
AGGTCTCCAACATATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCATTGAAAAAGC
TTATGAAGAAGGTATGGCTCAATTACCACGTCCAGATAATATTGAAGCTTTAGTCAAATC
TCGTCAATATGTTCCATACTTATGATAAATCAAAAAATTGACTATNCTTGCCCAATTTC
Length of connected seq. 949
Full length Seq ID -
Full length Seq. -
Length of full length seq. -