>SFH857 (SFH857Q) /CSM/SF/SFH8-C/SFH857Q.Seq.d/
XXXXXXXXXXTCCAGAGCTCTATGAGGAGATTGGTATCAAACCACCAAAGGGTGTCATCC
TCTATGGTGAGCCAGGTACTGGTAAGACTTTATTAGCCAAAGCCGTAGCAAATCAAACAT
CCGCTACATTTTTACGTGTTGTAGGCTCAGAATTAATTCAAAAGTATTTAGGTGATGGTC
CAAAATTAGTTCGTGAATTATTCAGAGTTGCAGATGAATGTGCACCATCAATTGTTTTCA
TTGATGAAATCGATGCTGTTGGTACCAAACGTTATGATTCTCAATCTGGTGGTGAACGTG
AAATTCAAAGAACTATGTTGGAACTCTTGAATCAATTGGATGGTTTCGATGCTAGAACCG
ATGTAAAAGTTATCATGGCTACAAATCGTATTGAAACTTTAGATCCTGCTCTCATTCGTC
CAGGTCGTATCGATCGTAAAATTGAATTCCCTCTTCCAGATATTAAGACCAAGAGAAAGA
TCTTTGAAATTCATACTGCTAAAATGAATCTCTCTGAAGATGTTAACCTTGAAGAGTTTG
TTATGTCTAAAGATGATTTATCCGGTGCTGATATCAAAGCCATCTGTACTGAATCTGGTC
TTTTAGCACTCAGAGAACGTAGAATGAGAGTAACTCATACNATTTTAAAAAAGCAAAAGA
AAAAGTTTTATACAGAAAAACAGCNGGTGCTCCAGAAGAGTTTATATA

---PELYEEIGIKPPKGVILYGEPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGP
KLVRELFRVADECAPSIVFIDEIDAVGTKRYDSQSGGEREIQRTMLELLNQLDGFDARTD
VKVIMATNRIETLDPALIRPGRIDRKIEFPLPDIKTKRKIFEIHTAKMNLSEDVNLEEFV
MSKDDLSGADIKAICTESGLLALRERRMRVTHTILKKQKKKFYTEKQXVLQKSLY

Frame A:
---PELYEEIGIKPPKGVILYGEPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGP
KLVRELFRVADECAPSIVFIDEIDAVGTKRYDSQSGGEREIQRTMLELLNQLDGFDARTD
VKVIMATNRIETLDPALIRPGRIDRKIEFPLPDIKTKRKIFEIHTAKMNLSEDVNLEEFV
MSKDDLSGADIKAICTESGLLALRERRMRVTHTILKKQKKKFYTEKQXVLQKSLY


Frame B:
---qssmrrlvsnhqrvsssmvsqvlvrly*pkp*qikhplhfyvl*aqn*fksi*vmvq
n*fvnyselqmnvhhqlfslmksmllvpnvmilnlvvnvkfkelcwns*inwmvsmlepm
*klswlqivlkl*illsfvqvvsivklnslfqilrprerslkfillk*islkmltlksll
clkmiypvliskpsvlnlvf*hsenve*e*lixf*kskrksfiqknsxcsrrvyi


Frame C:
---ral*gdwyqttkgchplw*aryw*dfisqsrsksniryiftccrlrinskvfr*wsk
iss*iiqscr*mctincfh**nrccwyqtl*fsiww*t*nsknyvgtlesigwfrc*nrc
ksyhgyksy*nfrscshssrsyrs*n*ipssry*dqekdl*nsyc*nesl*rc*p*rvcy
v*r*firc*yqshly*iwsfstqrt*nesnsyxfkkakekvlyrktagapeefi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH857 (SFH857Q) /CSM/SF/SFH8-C/SFH857Q.Seq.d/                       1255   0.0
AFN319 (AFN319Q) /CSM/AF/AFN3-A/AFN319Q.Seq.d/                       1235   0.0
AFE732 (AFE732Q) /CSM/AF/AFE7-B/AFE732Q.Seq.d/                       1170   0.0
SLB640 (SLB640Q) /CSM/SL/SLB6-B/SLB640Q.Seq.d/                       1061   0.0
FC-AJ15 (FC-AJ15Q) /CSM/FC/FC-AJ/FC-AJ15Q.Seq.d/                      626   e-178
AFC641 (AFC641Q) /CSM/AF/AFC6-B/AFC641Q.Seq.d/                        618   e-176
AFA160 (AFA160Q) /CSM/AF/AFA1-C/AFA160Q.Seq.d/                        613   e-174
SFE651 (SFE651Q) /CSM/SF/SFE6-C/SFE651Q.Seq.d/                        515   e-145
VFB722 (VFB722Q) /CSM/VF/VFB7-A/VFB722Q.Seq.d/                        509   e-143
AFB603 (AFB603Q) /CSM/AF/AFB6-A/AFB603Q.Seq.d/                        450   e-125

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE014830|AE014830.1 Plasmodium falciparum 3D7 chromosome 10 section 2 of 7 of the complete sequence.   188   2e-66  3
CA754855|CA754855.1 BR030007002_BR030_B09_66_076.ab1 OA Oryza sativa (japonica cultivar-group) cDNA clone BR030007002_BR030_B09_66_076.ab1 similar to agCP2907 [Anopheles gambiae str. PEST], mRNA sequence.   226   3e-65  4
BI741860|BI741860.1 kt81h06.y1 Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:PRS4_CAEEL O16368 PROBABLE 26S PROTEASE REGULATORY SUBUNIT 4. [1] ;, mRNA sequence.   163   4e-64  3
BU498573|BU498573.1 PfESToab99a02.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:PRS4_DROME P48601 26S PROTEASE REGULATORY SUBUNIT 4 ;, mRNA sequence.   155   4e-61  3
BU495626|BU495626.1 PfESToab75e05.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q9SL67 Q9SL67 26S PROTEASOME SUBUNIT 4. ;, mRNA sequence.   188   4e-61  2
BU495625|BU495625.1 PfESToab75e04.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q9SL67 Q9SL67 26S PROTEASOME SUBUNIT 4. ;, mRNA sequence.   188   4e-61  2
BI815241|BI815241.1 PfESToaa16c01.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:PRS4_HUMAN Q03527 26S PROTEASE REGULATORY SUBUNIT 4 ;, mRNA sequence.   188   4e-61  2
BI670760|BI670760.1 PfESToaa03h11.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:PRS4_ORYSA P46466 26S PROTEASE REGULATORY SUBUNIT 4 HOMOLOG ;, mRNA sequence.   125   7e-53  3
BM959492|BM959492.1 PLATE_9_C08_06.AB1 GS Lambda-Triplex, 10 day germinating spore library Glomus intraradices cDNA, mRNA sequence.   161   4e-52  3
BQ596467|BQ596467.1 PfESToab18c02.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:PRS4_ORYSA P46466 26S PROTEASE REGULATORY SUBUNIT 4 HOMOLOG ;, mRNA sequence.   117   6e-50  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FB781850_1(FB781850|pid:none) Sequence 1123 from Patent WO200803...   374   e-105
BT062241_1(BT062241|pid:none) Zea mays full-length cDNA clone ZM...   374   e-105
FN318152_1(FN318152|pid:none) Schistosoma japonicum isolate Anhu...   370   e-104
CP001325_99(CP001325|pid:none) Micromonas sp. RCC299 chromosome ...   369   e-103
DQ440313_1(DQ440313|pid:none) Aedes aegypti clone AET-397 26S pr...   369   e-103
(P48601) RecName: Full=26S protease regulatory subunit 4; AltNam...   369   e-103
AF123391_1(AF123391|pid:none) Arabidopsis thaliana 26S proteasom...   377   e-103
AB161192_1(AB161192|pid:none) Arabidopsis thaliana HLR mRNA for ...   377   e-103
AC006081_7(AC006081|pid:none) Arabidopsis thaliana chromosome 2 ...   376   e-103
D17789_1(D17789|pid:none) Oryza sativa Japonica Group mRNA for h...   375   e-103

 psg: 0.75  gvh: 0.46  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.16  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SFH857 is nuc

>SFH857Z.Seq
NNNNNNNNNNTCCAGAGCTCTATGAGGAGATTGGTATCAAACCACCAAAGGGTGTCATCC
TCTATGGTGAGCCAGGTACTGGTAAGACTTTATTAGCCAAAGCCGTAGCAAATCAAACAT
CCGCTACATTTTTACGTGTTGTAGGCTCAGAATTAATTCAAAAGTATTTAGGTGATGGTC
CAAAATTAGTTCGTGAATTATTCAGAGTTGCAGATGAATGTGCACCATCAATTGTTTTCA
TTGATGAAATCGATGCTGTTGGTACCAAACGTTATGATTCTCAATCTGGTGGTGAACGTG
AAATTCAAAGAACTATGTTGGAACTCTTGAATCAATTGGATGGTTTCGATGCTAGAACCG
ATGTAAAAGTTATCATGGCTACAAATCGTATTGAAACTTTAGATCCTGCTCTCATTCGTC
CAGGTCGTATCGATCGTAAAATTGAATTCCCTCTTCCAGATATTAAGACCAAGAGAAAGA
TCTTTGAAATTCATACTGCTAAAATGAATCTCTCTGAAGATGTTAACCTTGAAGAGTTTG
TTATGTCTAAAGATGATTTATCCGGTGCTGATATCAAAGCCATCTGTACTGAATCTGGTC
TTTTAGCACTCAGAGAACGTAGAATGAGAGTAACTCATACNATTTTAAAAAAGCAAAAGA
AAAAGTTTTATACAGAAAAACAGCNGGTGCTCCAGAAGAGTTTATATA