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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFA851
Library AF
(Link to library)
Clone ID AFA851
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12889-1
Original site URL
Representative seq. ID AFA851P
(Link to Original site)
Representative DNA sequence 
>AFA851 (AFA851Q) /CSM/AF/AFA8-C/AFA851Q.Seq.d/
ATTATAAAAAATAAATAATGTCAACAACAACAAATATTCAATTAAATAATTTAACAGCAA
TTTCACCAATTGATGGTAGATATTGGGGACAAGTTCAGGTATTAAGTGAATATTTTTCAG
AGTATGCATTAATTAAATATCGTGTTCAAGTTGAAATTGAATATTTCATTGAATTAAGTA
AACTTTCAGAATTGAAACCATTGAATGCAGTTAATAAGGAAGATGATCATAAGAAACTTA
GAGATATTTATTTACAATTTAAAGAGAGTGATGCACAAAAGATTAAACAAATTGAAAAAA
CCACAAATCATGATATAAAAGCAGTTGAATACTTTATCAAGGAGAAAATGCATACAGAAT
TACAATATAGTGAAGTTGTTACAGAGTTTATTCATTTCGGACTTACATCACAAGATATCA
ACAATACAGCCATACCATTATCAATCGTTGAATCAGTTGAGAAAGTATTAATTCCACAAT
TGAAACAATCAATCTTGGAACCATTGCGTCAATTTGCTCAACAATGGAAATCAATTCCAA
TGTTGGCACGTACCCATGGCCAACCAGCCACACCAACCACCGTCGGTAAGGAGCTAATGG
TTTTCATCGAACGTTTAGAGAACCAAATCAATCATTTGGAACAATCTGTACCACATACTT
GTAAATTTGGTGGTGCAACAGGTAATTTGAXXXXXXXXXXTTCTCATCGATTTTATGTCG
TGACATTTGGACCTACATCTCTATGGAGTATTTCAATCAAAAATTGGTCAAAGGTGAAGT
CGGTAGTAGTACTATGCCTCACAAGGTAAATCCAATCGATTTTGAAAATGCCGAAGGTAA
TATGGGTGTAGCCAATGCACTCTATGAACATTTATCCGCTAAATTACCAATCTCCCGTCT
TCAAAGAGATTTAACCGACTCAACTGTACTCCGTTCTATTGGTGTACCTTTCTCACATTC
TATCCTCTCCTTTAAATCAATTCAACGTGGTCTCTCAAAATTAGTACTCAATGAAGCAAA
TATTGCAAAAGATCTCGATGCAAATTGGGCTGTAGTTAGTGAAGCAATTCAAACTATCCT
TCGTCGTGAAGGTTTCCCAAAACCATATGAAGCCTTAAAAGAATTAACTCGTGTTAGTGG
TAGTAAAAAAATTACTGAATCTGATATTCAAACTTTTATCGATTCTTTATCAATTCCTGA
NATATTAAATCTNAATAAAACTTATACTCCTTTNA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ykk*IMSTTTNIQLNNLTAISPIDGRYWGQVQVLSEYFSEYALIKYRVQVEIEYFIELSK
LSELKPLNAVNKEDDHKKLRDIYLQFKESDAQKIKQIEKTTNHDIKAVEYFIKEKMHTEL
QYSEVVTEFIHFGLTSQDINNTAIPLSIVESVEKVLIPQLKQSILEPLRQFAQQWKSIPM
LARTHGQPATPTTVGKELMVFIERLENQINHLEQSVPHTCKFGGATGNL---

---FSSILCRDIWTYISMEYFNQKLVKGEVGSSTMPHKVNPIDFENAEGNMGVANALYEH
LSAKLPISRLQRDLTDSTVLRSIGVPFSHSILSFKSIQRGLSKLVLNEANIAKDLDANWA
VVSEAIQTILRREGFPKPYEALKELTRVSGSKKITESDIQTFIDSLSIPXILNLNKTYTP
X
Translated Amino Acid sequence (All Frames) 
Frame A:
iiknk*cqqqqifn*ii*qqfhqlmvdigdkfry*vnifqsmh*lnivfklklnisln*v
nfqn*nh*mqlirkmiirnleifiynlkrvmhkrlnklkkpqimi*kqlntlsrrkciqn
ynivkllqslfisdlhhkistiqpyhyqslnqlrky*fhn*nnqswnhcvnllnngnqfq
cwhvpmanqphqppsvrs*wfssnv*rtksiiwnnlyhilvnlvvqqvi*---

---FSSILCRDIWTYISMEYFNQKLVKGEVGSSTMPHKVNPIDFENAEGNMGVANALYEH
LSAKLPISRLQRDLTDSTVLRSIGVPFSHSILSFKSIQRGLSKLVLNEANIAKDLDANWA
VVSEAIQTILRREGFPKPYEALKELTRVSGSKKITESDIQTFIDSLSIPXILNLNKTYTP
X

Frame B:
l*kinnvnnnkysik*fnsnftn*w*ilgtssgik*iffrvcin*iscss*n*ifh*ik*
tfrietiecs**gr*s*et*rylfti*re*ctkd*tn*knhks*ykss*ilyqgenayri
ti**scyrvysfrtyitryqqyshtiinr*is*esinstietinlgtiasicstmeinsn
vgtypwptshtnhrr*gangfhrtfrepnqsfgticttyl*iwwcnr*f---

---shrfyvvtfgptslwsisiknwskvksvvvlcltr*iqsilkmpkviwv*pmhsmni
yplnyqspvfkei*ptqlysvllvylshilssplnqfnvvsqn*ysmkqilqkismqigl
*lvkqfklsfvvkvsqnhmkp*kn*lvlvvvkkllnlifkllsilyqflxy*ixiklill
x

Frame C:
ykk*IMSTTTNIQLNNLTAISPIDGRYWGQVQVLSEYFSEYALIKYRVQVEIEYFIELSK
LSELKPLNAVNKEDDHKKLRDIYLQFKESDAQKIKQIEKTTNHDIKAVEYFIKEKMHTEL
QYSEVVTEFIHFGLTSQDINNTAIPLSIVESVEKVLIPQLKQSILEPLRQFAQQWKSIPM
LARTHGQPATPTTVGKELMVFIERLENQINHLEQSVPHTCKFGGATGNL---

---lidfms*hldlhlygvfqskigqr*sr**yyasqgksnrf*kcrr*ygcsqctl*tf
ir*itnlpsskrfnrlnctpfywctfltfypll*instwslkistq*skyckrsrcklgc
s**snsnypss*rfpkti*slkrinsc*w**kny*i*ysnfyrffins*xiksx*nlysf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFA851 (AFA851Q) /CSM/AF/AFA8-C/AFA851Q.Seq.d/                       2331   0.0
AFA534 (AFA534Q) /CSM/AF/AFA5-B/AFA534Q.Seq.d/                       1310   0.0
AFL743 (AFL743Q) /CSM/AF/AFL7-B/AFL743Q.Seq.d/                       1207   0.0
VFJ122 (VFJ122Q) /CSM/VF/VFJ1-A/VFJ122Q.Seq.d/                       1183   0.0
SFD266 (SFD266Q) /CSM/SF/SFD2-C/SFD266Q.Seq.d/                       1112   0.0
CFJ829 (CFJ829Q) /CSM/CF/CFJ8-B/CFJ829Q.Seq.d/                       1108   0.0
SFH239 (SFH239Q) /CSM/SF/SFH2-B/SFH239Q.Seq.d/                       1106   0.0
AFE496 (AFE496Q) /CSM/AF/AFE4-D/AFE496Q.Seq.d/                       1100   0.0
AFJ289 (AFJ289Q) /CSM/AF/AFJ2-D/AFJ289Q.Seq.d/                       1092   0.0
AFI638 (AFI638Q) /CSM/AF/AFI6-B/AFI638Q.Seq.d/                       1053   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF262050|AF262050.1 Plasmodium yoelii YM adenylosuccinate lyase (asl) gene, complete cds.    66  3e-17  4
AF262049|AF262049.1 Plasmodium berghei ANKA adenylosuccinate lyase (asl) gene, complete cds.    50  9e-13  4
AF033037|AF033037.1 Plasmodium falciparum merozoite surface protein 4, merozoite surface protein 5, merozoite surface protein 2, and adenylosuccinate lyase genes, complete cds.    58  2e-10  2
AE001385|AE001385.1 Plasmodium falciparum chromosome 2, section 22 of 73 of the complete sequence.    58  3e-10  2
AF080446|AF080446.1 Plasmodium chabaudi adami merozoite surface protein 4/5 (MSP4/5) and adenylosuccinate lyase (ASL) genes, complete cds.    56  2e-08  3
AF080447|AF080447.2 Plasmodium chabaudi chabaudi asparagine-rich protein gene, partial cds; and merozoite surface protein 4/5 (MSP4/5) and adenylosuccinate lyase (ASL) genes, complete cds.    56  2e-07  3
AE014101|AE014101.1 Buchnera aphidicola str. Sg (Schizaphis graminum) section 26 of 57 of the complete genome.    44  6e-07  4
BU495443|BU495443.1 PfESToab73a04.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:O15918 O15918 ADENYLOSUCCINATE LYASE ;, mRNA sequence.    58  3e-04  1
BQ633344|BQ633344.1 PfESToab40g06.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:O15918 O15918 ADENYLOSUCCINATE LYASE ;, mRNA sequence.    58  3e-04  1
BU496038|BU496038.1 PfESToac03a07.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:O15918 O15918 ADENYLOSUCCINATE LYASE ;, mRNA sequence.    58  3e-04  1
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54J34) RecName: Full=Adenylosuccinate lyase;          Short=AS...   446   e-123
CP000746_27(CP000746|pid:none) Actinobacillus succinogenes 130Z,...   188   6e-95
AP006841_4011(AP006841|pid:none) Bacteroides fragilis YCH46 DNA,...   238   4e-61
CP000139_1577(CP000139|pid:none) Bacteroides vulgatus ATCC 8482,...   234   4e-60
CU207366_1075(CU207366|pid:none) Gramella forsetii KT0803 comple...   232   2e-59
AP010656_568(AP010656|pid:none) Candidatus Azobacteroides pseudo...   230   8e-59
AP009380_1220(AP009380|pid:none) Porphyromonas gingivalis ATCC 3...   222   2e-56
AM494941_49(AM494941|pid:none) Leishmania braziliensis chromosom...   220   9e-56
AL389894_46(AL389894|pid:none) Leishmania major Friedlin chromos...   219   1e-55
AM502222_45(AM502222|pid:none) Leishmania infantum chromosome 4....   217   6e-55
protein update 2009. 3.26
PSORT 

 psg: 0.85  gvh: 0.50  alm: 0.43  top: 0.40  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for AFA851 is nuc
5' end seq. ID AFA851F
5' end seq. 
>AFA851F.Seq
ATTATAAAAAATAAATAATGTCAACAACAACAAATATTCAATTAAATAATTTAACAGCAA
TTTCACCAATTGATGGTAGATATTGGGGACAAGTTCAGGTATTAAGTGAATATTTTTCAG
AGTATGCATTAATTAAATATCGTGTTCAAGTTGAAATTGAATATTTCATTGAATTAAGTA
AACTTTCAGAATTGAAACCATTGAATGCAGTTAATAAGGAAGATGATCATAAGAAACTTA
GAGATATTTATTTACAATTTAAAGAGAGTGATGCACAAAAGATTAAACAAATTGAAAAAA
CCACAAATCATGATATAAAAGCAGTTGAATACTTTATCAAGGAGAAAATGCATACAGAAT
TACAATATAGTGAAGTTGTTACAGAGTTTATTCATTTCGGACTTACATCACAAGATATCA
ACAATACAGCCATACCATTATCAATCGTTGAATCAGTTGAGAAAGTATTAATTCCACAAT
TGAAACAATCAATCTTGGAACCATTGCGTCAATTTGCTCAACAATGGAAATCAATTCCAA
TGTTGGCACGTACCCATGGCCAACCAGCCACACCAACCACCGTCGGTAAGGAGCTAATGG
TTTTCATCGAACGTTTAGAGAACCAAATCAATCATTTGGAACAATCTGTACCACATACTT
GTAAATTTGGTGGTGCAACAGGTAATTTGA----------
Length of 5' end seq. 690
3' end seq. ID AFA851Z
3' end seq. 
>AFA851Z.Seq
----------TTCTCATCGATTTTATGTCGTGACATTTGGACCTACATCTCTATGGAGTA
TTTCAATCAAAAATTGGTCAAAGGTGAAGTCGGTAGTAGTACTATGCCTCACAAGGTAAA
TCCAATCGATTTTGAAAATGCCGAAGGTAATATGGGTGTAGCCAATGCACTCTATGAACA
TTTATCCGCTAAATTACCAATCTCCCGTCTTCAAAGAGATTTAACCGACTCAACTGTACT
CCGTTCTATTGGTGTACCTTTCTCACATTCTATCCTCTCCTTTAAATCAATTCAACGTGG
TCTCTCAAAATTAGTACTCAATGAAGCAAATATTGCAAAAGATCTCGATGCAAATTGGGC
TGTAGTTAGTGAAGCAATTCAAACTATCCTTCGTCGTGAAGGTTTCCCAAAACCATATGA
AGCCTTAAAAGAATTAACTCGTGTTAGTGGTAGTAAAAAAATTACTGAATCTGATATTCA
AACTTTTATCGATTCTTTATCAATTCCTGANATATTAAATCTNAATAAAACTTATACTCC
TTTNA
Length of 3' end seq. 535
Connected seq. ID AFA851P
Connected seq. 
>AFA851P.Seq
ATTATAAAAAATAAATAATGTCAACAACAACAAATATTCAATTAAATAATTTAACAGCAA
TTTCACCAATTGATGGTAGATATTGGGGACAAGTTCAGGTATTAAGTGAATATTTTTCAG
AGTATGCATTAATTAAATATCGTGTTCAAGTTGAAATTGAATATTTCATTGAATTAAGTA
AACTTTCAGAATTGAAACCATTGAATGCAGTTAATAAGGAAGATGATCATAAGAAACTTA
GAGATATTTATTTACAATTTAAAGAGAGTGATGCACAAAAGATTAAACAAATTGAAAAAA
CCACAAATCATGATATAAAAGCAGTTGAATACTTTATCAAGGAGAAAATGCATACAGAAT
TACAATATAGTGAAGTTGTTACAGAGTTTATTCATTTCGGACTTACATCACAAGATATCA
ACAATACAGCCATACCATTATCAATCGTTGAATCAGTTGAGAAAGTATTAATTCCACAAT
TGAAACAATCAATCTTGGAACCATTGCGTCAATTTGCTCAACAATGGAAATCAATTCCAA
TGTTGGCACGTACCCATGGCCAACCAGCCACACCAACCACCGTCGGTAAGGAGCTAATGG
TTTTCATCGAACGTTTAGAGAACCAAATCAATCATTTGGAACAATCTGTACCACATACTT
GTAAATTTGGTGGTGCAACAGGTAATTTGA----------TTCTCATCGATTTTATGTCG
TGACATTTGGACCTACATCTCTATGGAGTATTTCAATCAAAAATTGGTCAAAGGTGAAGT
CGGTAGTAGTACTATGCCTCACAAGGTAAATCCAATCGATTTTGAAAATGCCGAAGGTAA
TATGGGTGTAGCCAATGCACTCTATGAACATTTATCCGCTAAATTACCAATCTCCCGTCT
TCAAAGAGATTTAACCGACTCAACTGTACTCCGTTCTATTGGTGTACCTTTCTCACATTC
TATCCTCTCCTTTAAATCAATTCAACGTGGTCTCTCAAAATTAGTACTCAATGAAGCAAA
TATTGCAAAAGATCTCGATGCAAATTGGGCTGTAGTTAGTGAAGCAATTCAAACTATCCT
TCGTCGTGAAGGTTTCCCAAAACCATATGAAGCCTTAAAAGAATTAACTCGTGTTAGTGG
TAGTAAAAAAATTACTGAATCTGATATTCAAACTTTTATCGATTCTTTATCAATTCCTGA
NATATTAAATCTNAATAAAACTTATACTCCTTTNA
Length of connected seq. 1225
Full length Seq ID -
Full length Seq. -
Length of full length seq. -