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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI409
Library AF
(Link to library)
Clone ID AFI409
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U08780-1
Original site URL
Representative seq. ID AFI409P
(Link to Original site)
Representative DNA sequence 
>AFI409 (AFI409Q) /CSM/AF/AFI4-A/AFI409Q.Seq.d/
TGGCCTACTGGTAAAAAGNATAATTATTATTATTTTTCATAAAATAATAGAAAAATGATT
GAAGAACATAATTGTTTTGAAGAATTTATAAATACAGCAGCAATTGGTGAACAACAAGTT
TATGGTGAATATAAATCTAAATTAGATTCATTTACAGATGAAGGTAAATTACCATATGAA
TTATTATTAGAGAGAAAGAATTTAGTAGAATTGAAAATTTCTGATGAAGAATATAAAAAG
AGTGAATCTGCATTAAAAACACCAAATCCAACATTATTACAAAAGTTTCAAATTAATTTT
TATCAAACTATTGAATCGATTAATCAAGTATTTAAACCATCATCATCATCATCAAATCAA
TCATCATCATCGTCCTCCTCGAATGAAGATGATTCATTACAAAATAAAAAGATTAAAGTA
ATGTCATGGCATCCACAACATAGATTACTTGCAGTTTGTAATAAAAATGATGTTATTTAT
ATCTACTATTTCCCAAATCAAGATATGTATGGTATGGGTAATGTCAGACCTTTAACATTA
TGGTTTGAATTACAATCAAAAGTTTATGATATACAATGGAAACCTTTCTTACCATATACT
TTAGCTGNTGCTTGNGAAAATGGNNTAATTNTATGXXXXXXXXXXTATCCATACTTTTTA
TCAAATACAATTACATGGTCATCAAATGGTTTACAATTAGCATGTGGTTCAACTAATCAT
AGTTCAATTCTATTATGGGATGTTGTATCTAGAGTACCAACATTCATACCACGTTATAAT
GGTAACTCATTATTAGTTTGGTCTCCAAAAAATGATTATCTTTTATCTTGTGGTGGTAAA
ACTTGTAGAATTTTCGATATCTCAAAATGGGATTACAATAATAAAGAATGGCCAATGTTA
ACAAATTATCAAACAGGATCATGGAATGGAAAAGGTGAATATTTAGCAGTTGCAAGTGGT
GATAGAATTCAGTTTATTCAATGTATAAATAGAGCATTTGAATCAGGTGGTGAATTAATG
TATGTTGAGAAAACTTCAACCTATAGATTACAAAATGTTGGTGGTAGTGGTGGTGGTTCA
AGTAGTAGTGAATTATTTTTAGGCGGACATATAAAGAAAATTGCATGGTCACCTGATAGT
CAAAGATTGGCTGTCATCTTTTATTTAAATAGCAAGAGTAGAAATAATGATACTTATTGT
GCAATCTATAAAATTAAAACTTATCCTAAATTTTCTGTAACACCAAGAGGTTTCATAAGG
TCAAAAGATCAATCAATTCAATCAGTTTCTTTTGTCCCAAATTATAAAAAAGGTTCTTTA
TTATCAATTGCCTACAATGATGGAACAATTAAATTTTTCCCATTATTATATCAATATAAA
CAATAAGAAAATAAAGATTTTACACTTTATTATCATTA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
wptgkkxnyyyfs*NNRKMIEEHNCFEEFINTAAIGEQQVYGEYKSKLDSFTDEGKLPYE
LLLERKNLVELKISDEEYKKSESALKTPNPTLLQKFQINFYQTIESINQVFKPSSSSSNQ
SSSSSSSNEDDSLQNKKIKVMSWHPQHRLLAVCNKNDVIYIYYFPNQDMYGMGNVRPLTL
WFELQSKVYDIQWKPFLPYTLAXAXENGXIX---

---YPYFLSNTITWSSNGLQLACGSTNHSSILLWDVVSRVPTFIPRYNGNSLLVWSPKND
YLLSCGGKTCRIFDISKWDYNNKEWPMLTNYQTGSWNGKGEYLAVASGDRIQFIQCINRA
FESGGELMYVEKTSTYRLQNVGGSGGGSSSSELFLGGHIKKIAWSPDSQRLAVIFYLNSK
SRNNDTYCAIYKIKTYPKFSVTPRGFIRSKDQSIQSVSFVPNYKKGSLLSIAYNDGTIKF
FPLLYQYKQ*enkdftlyyh
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgkkxnyyyfs*NNRKMIEEHNCFEEFINTAAIGEQQVYGEYKSKLDSFTDEGKLPYE
LLLERKNLVELKISDEEYKKSESALKTPNPTLLQKFQINFYQTIESINQVFKPSSSSSNQ
SSSSSSSNEDDSLQNKKIKVMSWHPQHRLLAVCNKNDVIYIYYFPNQDMYGMGNVRPLTL
WFELQSKVYDIQWKPFLPYTLAXAXENGXIX---

---YPYFLSNTITWSSNGLQLACGSTNHSSILLWDVVSRVPTFIPRYNGNSLLVWSPKND
YLLSCGGKTCRIFDISKWDYNNKEWPMLTNYQTGSWNGKGEYLAVASGDRIQFIQCINRA
FESGGELMYVEKTSTYRLQNVGGSGGGSSSSELFLGGHIKKIAWSPDSQRLAVIFYLNSK
SRNNDTYCAIYKIKTYPKFSVTPRGFIRSKDQSIQSVSFVPNYKKGSLLSIAYNDGTIKF
FPLLYQYKQ*enkdftlyyh

Frame B:
gllvkxiiiiifhkiiek*lkniivlknl*iqqqlvnnkfmvninln*ihlqmkvnyhmn
yy*reri**n*kflmknikrvnlh*khqiqhyyksfklifikllnrlikylnhhhhhqin
hhhrpprmkmihykikrlk*chgihnidylqfvikmmlfistisqikicmvwvmsdl*hy
glnynqkfmiyngnlsyhil*lxlxkmx*xy---

---ihtfyqiqlhghqmvyn*hvvqliivqfyygmlyleyqhsyhvimvthy*fglqkmi
ifylvvvklvefsisqngitiikngqc*qiikqdhgmekvni*qlqvviefslfnv*ieh
lnqvvn*cmlrklqpidykmlvvvvvvqvvvnyf*adi*rklhghlivkdwlssfi*iar
veimilivqsiklklilnfl*hqevs*gqkinqfnqfllsqiikkvlyyqlptmmeqlnf
shyyininnkkikilhfiii

Frame C:
ayw*kx*lllffik**knd*rt*lf*riykyssnw*ttslw*i*i*irfiyr*r*iti*i
iirekefsrienf**ri*ke*icikntksniitkvsn*flsny*id*ssi*tiiiiiksi
iiivlle*r*fitk*kd*snvmastt*itcsl**k*cylyllfpksryvwyg*cqtfnim
v*itiksl*ytmetfltiyfsxclxkwxnxm---

---silfikynymvikwftismwfn*s*fnsimgcci*stnihttl*w*liislvskk*l
sfilww*nl*nfrylkmglq**rmanvnklsnrimewkr*ifssckw**nsvysmyk*si
*irw*invc*enfnl*itkcww*wwwfk***iifrrtykencmvt**skigchllfk*qe
*k**yllcnl*n*nls*ifcntkrfhkvkrsinsisffcpkl*krffiinclq*wnn*if
piiisi*tirk*rfytllsl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI409 (AFI409Q) /CSM/AF/AFI4-A/AFI409Q.Seq.d/                       1986   0.0
VFI871 (VFI871Q) /CSM/VF/VFI8-C/VFI871Q.Seq.d/                       1477   0.0
AFH742 (AFH742Q) /CSM/AF/AFH7-B/AFH742Q.Seq.d/                       1475   0.0
SSK827 (SSK827Q) /CSM/SS/SSK8-B/SSK827Q.Seq.d/                       1318   0.0
CFG838 (CFG838Q) /CSM/CF/CFG8-B/CFG838Q.Seq.d/                       1221   0.0
CFG822 (CFG822Q) /CSM/CF/CFG8-A/CFG822Q.Seq.d/                        553   e-156
SSE416 (SSE416Q) /CSM/SS/SSE4-A/SSE416Q.Seq.d/                        170   4e-41
VSB478 (VSB478Q) /CSM/VS/VSB4-D/VSB478Q.Seq.d/                        105   2e-21
VSC172 (VSC172Q) /CSM/VS/VSC1-C/VSC172Q.Seq.d/                         46   0.001
VHH302 (VHH302Q) /CSM/VH/VHH3-A/VHH302Q.Seq.d/                         46   0.001
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115581|AC115581.3 Dictyostelium discoideum chromosome 2 map complement(3527391-3470188) strain AX4, complete sequence.   829   0.0    10
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   2e-13  20
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    38   3e-12  18
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    40   1e-11  19
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            42   4e-11  22
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    38   3e-10  24
AE014824|AE014824.1 Plasmodium falciparum 3D7 chromosome 14 section 9 of 13 of the complete sequence.    34   3e-10  22
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           38   9e-10  21
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    40   1e-09  21
AE014847|AE014847.1 Plasmodium falciparum 3D7 chromosome 12, section 4 of 9 of the complete sequence.    34   5e-09  20
dna update 2004. 6.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115581_9(AC115581|pid:none) Dictyostelium discoideum chromosom...   822   0.0
BC077988_1(BC077988|pid:none) Xenopus laevis achalasia, adrenoco...   103   2e-20
AK088247_1(AK088247|pid:none) Mus musculus 2 days neonate thymus...    95   5e-18
BC067671_1(BC067671|pid:none) Danio rerio achalasia, adrenocorti...    93   3e-17
AY891872_1(AY891872|pid:none) Synthetic construct Homo sapiens c...    91   7e-17
AK222509_1(AK222509|pid:none) Homo sapiens mRNA for achalasia, a...    91   7e-17
(Q9NRG9) RecName: Full=Aladin; AltName: Full=Adracalin;  &AF2260...    91   7e-17
AK000833_1(AK000833|pid:none) Homo sapiens cDNA FLJ20826 fis, cl...    91   1e-16
BC120418_1(BC120418|pid:none) Bos taurus achalasia, adrenocortic...    91   1e-16
BT067274_1(BT067274|pid:none) Zea mays full-length cDNA clone ZM...    81   8e-14
protein update 2009. 4.23
PSORT 

 psg: 0.63  gvh: 0.36  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for AFI409 is nuc
5' end seq. ID AFI409F
5' end seq. 
>AFI409F.Seq
TGGCCTACTGGTAAAAAGNATAATTATTATTATTTTTCATAAAATAATAGAAAAATGATT
GAAGAACATAATTGTTTTGAAGAATTTATAAATACAGCAGCAATTGGTGAACAACAAGTT
TATGGTGAATATAAATCTAAATTAGATTCATTTACAGATGAAGGTAAATTACCATATGAA
TTATTATTAGAGAGAAAGAATTTAGTAGAATTGAAAATTTCTGATGAAGAATATAAAAAG
AGTGAATCTGCATTAAAAACACCAAATCCAACATTATTACAAAAGTTTCAAATTAATTTT
TATCAAACTATTGAATCGATTAATCAAGTATTTAAACCATCATCATCATCATCAAATCAA
TCATCATCATCGTCCTCCTCGAATGAAGATGATTCATTACAAAATAAAAAGATTAAAGTA
ATGTCATGGCATCCACAACATAGATTACTTGCAGTTTGTAATAAAAATGATGTTATTTAT
ATCTACTATTTCCCAAATCAAGATATGTATGGTATGGGTAATGTCAGACCTTTAACATTA
TGGTTTGAATTACAATCAAAAGTTTATGATATACAATGGAAACCTTTCTTACCATATACT
TTAGCTGNTGCTTGNGAAAATGGNNTAATTNTATG----------
Length of 5' end seq. 635
3' end seq. ID AFI409Z
3' end seq. 
>AFI409Z.Seq
----------TATCCATACTTTTTATCAAATACAATTACATGGTCATCAAATGGTTTACA
ATTAGCATGTGGTTCAACTAATCATAGTTCAATTCTATTATGGGATGTTGTATCTAGAGT
ACCAACATTCATACCACGTTATAATGGTAACTCATTATTAGTTTGGTCTCCAAAAAATGA
TTATCTTTTATCTTGTGGTGGTAAAACTTGTAGAATTTTCGATATCTCAAAATGGGATTA
CAATAATAAAGAATGGCCAATGTTAACAAATTATCAAACAGGATCATGGAATGGAAAAGG
TGAATATTTAGCAGTTGCAAGTGGTGATAGAATTCAGTTTATTCAATGTATAAATAGAGC
ATTTGAATCAGGTGGTGAATTAATGTATGTTGAGAAAACTTCAACCTATAGATTACAAAA
TGTTGGTGGTAGTGGTGGTGGTTCAAGTAGTAGTGAATTATTTTTAGGCGGACATATAAA
GAAAATTGCATGGTCACCTGATAGTCAAAGATTGGCTGTCATCTTTTATTTAAATAGCAA
GAGTAGAAATAATGATACTTATTGTGCAATCTATAAAATTAAAACTTATCCTAAATTTTC
TGTAACACCAAGAGGTTTCATAAGGTCAAAAGATCAATCAATTCAATCAGTTTCTTTTGT
CCCAAATTATAAAAAAGGTTCTTTATTATCAATTGCCTACAATGATGGAACAATTAAATT
TTTCCCATTATTATATCAATATAAACAATAAGAAAATAAAGATTTTACACTTTATTATCA
TTA
Length of 3' end seq. 773
Connected seq. ID AFI409P
Connected seq. 
>AFI409P.Seq
TGGCCTACTGGTAAAAAGNATAATTATTATTATTTTTCATAAAATAATAGAAAAATGATT
GAAGAACATAATTGTTTTGAAGAATTTATAAATACAGCAGCAATTGGTGAACAACAAGTT
TATGGTGAATATAAATCTAAATTAGATTCATTTACAGATGAAGGTAAATTACCATATGAA
TTATTATTAGAGAGAAAGAATTTAGTAGAATTGAAAATTTCTGATGAAGAATATAAAAAG
AGTGAATCTGCATTAAAAACACCAAATCCAACATTATTACAAAAGTTTCAAATTAATTTT
TATCAAACTATTGAATCGATTAATCAAGTATTTAAACCATCATCATCATCATCAAATCAA
TCATCATCATCGTCCTCCTCGAATGAAGATGATTCATTACAAAATAAAAAGATTAAAGTA
ATGTCATGGCATCCACAACATAGATTACTTGCAGTTTGTAATAAAAATGATGTTATTTAT
ATCTACTATTTCCCAAATCAAGATATGTATGGTATGGGTAATGTCAGACCTTTAACATTA
TGGTTTGAATTACAATCAAAAGTTTATGATATACAATGGAAACCTTTCTTACCATATACT
TTAGCTGNTGCTTGNGAAAATGGNNTAATTNTATG----------TATCCATACTTTTTA
TCAAATACAATTACATGGTCATCAAATGGTTTACAATTAGCATGTGGTTCAACTAATCAT
AGTTCAATTCTATTATGGGATGTTGTATCTAGAGTACCAACATTCATACCACGTTATAAT
GGTAACTCATTATTAGTTTGGTCTCCAAAAAATGATTATCTTTTATCTTGTGGTGGTAAA
ACTTGTAGAATTTTCGATATCTCAAAATGGGATTACAATAATAAAGAATGGCCAATGTTA
ACAAATTATCAAACAGGATCATGGAATGGAAAAGGTGAATATTTAGCAGTTGCAAGTGGT
GATAGAATTCAGTTTATTCAATGTATAAATAGAGCATTTGAATCAGGTGGTGAATTAATG
TATGTTGAGAAAACTTCAACCTATAGATTACAAAATGTTGGTGGTAGTGGTGGTGGTTCA
AGTAGTAGTGAATTATTTTTAGGCGGACATATAAAGAAAATTGCATGGTCACCTGATAGT
CAAAGATTGGCTGTCATCTTTTATTTAAATAGCAAGAGTAGAAATAATGATACTTATTGT
GCAATCTATAAAATTAAAACTTATCCTAAATTTTCTGTAACACCAAGAGGTTTCATAAGG
TCAAAAGATCAATCAATTCAATCAGTTTCTTTTGTCCCAAATTATAAAAAAGGTTCTTTA
TTATCAATTGCCTACAATGATGGAACAATTAAATTTTTCCCATTATTATATCAATATAAA
CAATAAGAAAATAAAGATTTTACACTTTATTATCATTA
Length of connected seq. 1408
Full length Seq ID -
Full length Seq. -
Length of full length seq. -