>SFH636 (SFH636Q) /CSM/SF/SFH6-B/SFH636Q.Seq.d/
ACTGTTGGCCTACTGGACTTCCCCAAAATTTAAATATATCAATTTGTTTTTAACAAAAAC
ATTATTGGTTTTTTTATTTCATAATTTAAATAGTTTTTTTTTAAAAAAACTTTAATATAA
AAGATTCTCAATTTATATATTTTATAATATTACAATGAATACAATATTTACAATTATTTT
AACAATTACAATATTAGTATTATCATTAATATTAAAAGATTTATTATTTGAAGGTAGGAT
TAAAAAAATTAATAAATTAATACCTGGTCCTTCTACAATTCCAGTTTTTGGTAATTTATT
ACAAATTAACGCAAAAGATTTCCCAAAAAGTGTAAATGATTTCTATGAAAGATATGGCAA
AGTTTTTAGATTAAGATTGGGTAGTGTTGAAATTGTTGTTCTAACTGGACCTGAAGTTAT
TGATGAATGTTTTAATAAAAAACATAGAGAAATTTTCAAAGAAAGATATATTAAATTCTC
AAGATTTTTGGGTAAAGATTATAATATTTTTTCTXXXXXXXXXX

llaywtspkfkyinlfltktllvflfhnlnsfflkkl*YKRFSIYIFYNITMNTIFTIIL
TITILVLSLILKDLLFEGRIKKINKLIPGPSTIPVFGNLLQINAKDFPKSVNDFYERYGK
VFRLRLGSVEIVVLTGPEVIDECFNKKHREIFKERYIKFSRFLGKDYNIF---

Frame A:
tvglldfpki*iyqfvfnkniigffis*fk*fffkktli*kilnlyil*yyneyniynyf
nnynisiiinikrfii*r*d*kn**intwsfynssfw*fitn*rkrfpkkck*fl*kiwq
sf*ikig*c*nccsnwt*sy**mf**kt*rnfqrkiy*ilkifg*rl*yff---


Frame B:
llaywtspkfkyinlfltktllvflfhnlnsfflkkl*YKRFSIYIFYNITMNTIFTIIL
TITILVLSLILKDLLFEGRIKKINKLIPGPSTIPVFGNLLQINAKDFPKSVNDFYERYGK
VFRLRLGSVEIVVLTGPEVIDECFNKKHREIFKERYIKFSRFLGKDYNIF---


Frame C:
cwptglpqnlnisicf*qkhywffyfii*ivff*knfnikdsqfiyfiilq*iqylqlf*
qlqy*yyh*y*kiyylkvglkklin*ylvllqfqflviyykltqkisqkv*mismkdmak
fld*dwvvlkllf*ldlkllmnvlikniekfskkdilnsqdfwvkiiiff---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH636 (SFH636Q) /CSM/SF/SFH6-B/SFH636Q.Seq.d/                        527   e-149
CHD507 (CHD507Q) /CSM/CH/CHD5-A/CHD507Q.Seq.d/                         42   0.008
CHB524 (CHB524Q) /CSM/CH/CHB5-A/CHB524Q.Seq.d/                         42   0.008
VFC337 (VFC337Q) /CSM/VF/VFC3-B/VFC337Q.Seq.d/                         40   0.033
VFC317 (VFC317Q) /CSM/VF/VFC3-A/VFC317Q.Seq.d/                         40   0.033
SFJ342 (SFJ342Q) /CSM/SF/SFJ3-B/SFJ342Q.Seq.d/                         40   0.033
SFG478 (SFG478Q) /CSM/SF/SFG4-D/SFG478Q.Seq.d/                         40   0.033
SFC292 (SFC292Q) /CSM/SF/SFC2-D/SFC292Q.Seq.d/                         40   0.033
CHB644 (CHB644Q) /CSM/CH/CHB6-B/CHB644Q.Seq.d/                         40   0.033
AFH124 (AFH124Q) /CSM/AF/AFH1-A/AFH124Q.Seq.d/                         40   0.033

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE014831|AE014831.1 Plasmodium falciparum 3D7 chromosome 10 section 3 of 7 of thecomplete sequence.    34   1e-05  13
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    36   4e-05  12
AE014846|AE014846.1 Plasmodium falciparum 3D7 chromosome 12, section 3 of 9 of the complete sequence.    32   2e-04  12
Z11874|Z11874.1 Euglena gracilis Z chloroplast DNA.                34   4e-04  7
AP004170|AP004170.1 Mycoplasma penetrans genomic DNA, complete sequence, section 1/5.    34   6e-04  11
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    30   6e-04  13
AE014822|AE014822.1 Plasmodium falciparum 3D7 chromosome 14 section 7 of 13 of the complete sequence.    34   0.001  13
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34   0.001  14
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    32   0.001  13
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           32   0.001  23

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXI7) RecName: Full=Probable cytochrome P450 513F1;          ...    80   2e-14
BC129616_1(BC129616|pid:none) Xenopus laevis hypothetical protei...    63   5e-09
(Q54SV9) RecName: Full=Probable cytochrome P450 515B1;          ...    61   1e-08
BC089790_1(BC089790|pid:none) Rattus norvegicus cytochrome P450,...    61   2e-08
(Q54WB9) RecName: Full=Probable cytochrome P450 513D1;          ...    60   2e-08
AB198059_1(AB198059|pid:none) Felis catus CYP2E2 variant 2 mRNA ...    60   2e-08
BT053208_1(BT053208|pid:none) Medicago truncatula clone MTYFL_FM...    60   2e-08
AB198058_1(AB198058|pid:none) Felis catus CYP2E2 variant 1 mRNA ...    60   2e-08
AB290008_1(AB290008|pid:none) Balaenoptera acutorostrata CYP2C78...    60   3e-08
AB109547_1(AB109547|pid:none) Balaenoptera acutorostrata CYP2C m...    60   3e-08

 psg: 0.98  gvh: 0.59  alm: 0.14  top: 0.53  tms: 0.07  mit: 0.44  mip: 0.02
 nuc: 0.02  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.88  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: mitochondrial
	 24.0 %: Golgi
	 12.0 %: cytoplasmic
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for SFH636 is mit

>SFH636F.Seq
ACTGTTGGCCTACTGGACTTCCCCAAAATTTAAATATATCAATTTGTTTTTAACAAAAAC
ATTATTGGTTTTTTTATTTCATAATTTAAATAGTTTTTTTTTAAAAAAACTTTAATATAA
AAGATTCTCAATTTATATATTTTATAATATTACAATGAATACAATATTTACAATTATTTT
AACAATTACAATATTAGTATTATCATTAATATTAAAAGATTTATTATTTGAAGGTAGGAT
TAAAAAAATTAATAAATTAATACCTGGTCCTTCTACAATTCCAGTTTTTGGTAATTTATT
ACAAATTAACGCAAAAGATTTCCCAAAAAGTGTAAATGATTTCTATGAAAGATATGGCAA
AGTTTTTAGATTAAGATTGGGTAGTGTTGAAATTGTTGTTCTAACTGGACCTGAAGTTAT
TGATGAATGTTTTAATAAAAAACATAGAGAAATTTTCAAAGAAAGATATATTAAATTCTC
AAGATTTTTGGGTAAAGATTATAATATTTTTTCT----------