>AFI386 (AFI386Q) /CSM/AF/AFI3-D/AFI386Q.Seq.d/
XXXXXXXXXXCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTA
AAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATG
AAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTA
AAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGG
TTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTG
AATTCTACGCTCCATGGTGCAGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAG
GTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATG
ATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATG
ACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAAT
TCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGATAATG
TTGAATCTAAAAAAGATTCTTCTGCTAAACATGTATGNATTATAAATA

---DMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGK
VSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCSHCKNLAPIYDKLG
EYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEF
IQDNAAIEFKLPSSQTDDNVESKKDSSAKHVXIIN

Frame A:
---DMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGK
VSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCSHCKNLAPIYDKLG
EYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEF
IQDNAAIEFKLPSSQTDDNVESKKDSSAKHVXIIN


Frame B:
---i*rnshnkqliwvslvklfqpslliqlpiklvtismkkkpshsipspngskmslvvk
flhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*v
nt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tllns
fkitlplnsnyhhlklmimlnlkkilllnmyxl*i


Frame C:
---yeeipttsnsygslw*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*s
fsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmvqslqescsnlr*ir*
ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*ih
sr*rch*iqitiisn***c*i*krffc*tcmxyk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI386 (AFI386Q) /CSM/AF/AFI3-D/AFI386Q.Seq.d/                       1259   0.0
AFI355 (AFI355Q) /CSM/AF/AFI3-C/AFI355Q.Seq.d/                       1245   0.0
AFN839 (AFN839Q) /CSM/AF/AFN8-B/AFN839Q.Seq.d/                       1235   0.0
VFM432 (VFM432Q) /CSM/VF/VFM4-B/VFM432Q.Seq.d/                       1227   0.0
AHO764 (AHO764Q) /CSM/AH/AHO7-C/AHO764Q.Seq.d/                       1227   0.0
AFO829 (AFO829Q) /CSM/AF/AFO8-B/AFO829Q.Seq.d/                       1227   0.0
AFN252 (AFN252Q) /CSM/AF/AFN2-C/AFN252Q.Seq.d/                       1227   0.0
AFM402 (AFM402Q) /CSM/AF/AFM4-A/AFM402Q.Seq.d/                       1227   0.0
AFL789 (AFL789Q) /CSM/AF/AFL7-D/AFL789Q.Seq.d/                       1227   0.0
AFL565 (AFL565Q) /CSM/AF/AFL5-C/AFL565Q.Seq.d/                       1227   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   591   e-165  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    66   7e-07  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    66   7e-07  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    66   7e-07  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    66   7e-07  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    62   1e-05  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    62   1e-05  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     62   1e-05  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    62   1e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   166   7e-40
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   160   2e-38
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   159   5e-38
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   158   1e-37
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   154   2e-36
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   152   8e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   149   6e-35
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   149   6e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   148   1e-34
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   147   2e-34

 psg: 0.84  gvh: 0.38  alm: 0.43  top: 0.43  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFI386 is cyt

>AFI386Z.Seq
----------CGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTA
AAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATG
AAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTA
AAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGG
TTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTG
AATTCTACGCTCCATGGTGCAGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAG
GTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATG
ATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATG
ACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAAT
TCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGATAATG
TTGAATCTAAAAAAGATTCTTCTGCTAAACATGTATGNATTATAAATA