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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFH530
Library SF
(Link to library)
Clone ID SFH530
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16368-1
Original site URL
Representative seq. ID SFH530P
(Link to Original site)
Representative DNA sequence 
>SFH530 (SFH530Q) /CSM/SF/SFH5-B/SFH530Q.Seq.d/
ACTGTTGGCCTACTGGNATTCATTCAATTTTGTAATTATAAAAAAGGAATCAAATAATTA
TATATATATATAAGAAATATAAAAAAAATAAAATGAGNAATTTTATATTTAGCTTCTCTT
TTATTTTTAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGT
GAAAGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTT
GTATCATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGT
GATGATTNATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGT
TGTAAACCACATAGAGATTTTGTACCATATCATCTCACCCAAATACAACCACATGTGAAT
CAATTAGGTTGTGAAGCAAGAGGTATGGGAATGTGAATGGGNTXXXXXXXXXXGNTGNNC
ATNTCATNCAATTTGTATTCATCCATCAANTNNTGNTGCTTCAACCATTGCAACAACTGC
ATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAACTGGTGGAACTAC
AACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCGCTGCCTC
CTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGTAGCGC
CGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTCTGTCTCAAGTAG
TGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGC
sequence update 2001.11.22
Translated Amino Acid sequence 
llaywxsfnfviikkesnnyiyi*ei*kk*nexfyi*llfyf*lhyiyhqllvgvvaeiv
kvivqnihvnqievvhiyhlyhvvvrknfsvliviieivvmixhvlktrelvkslrfgqv
vnhieilyhiispkynhm*in*VVKQEVWECEWX---

---XXHXXQFVFIHQXXXLQPLQQLHQLSQLQPLQLLQVQQLVELQLVVQLLIVVLLPQP
IVALPPHHHQAVLLQVPLQVNHQVAPLQVVLHPLLQVALHPLSQVVLHHHQLQVQLHHQ
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxfiqfcnykkgik*lyiyirnikkik*xilylasllflitlylsptfgwgggrdc
eshrseytcksdrscaylpfvsccgkkeffcvnrdhrnccddxscakntrtgeifeiwss
ckphrdfvpyhltqiqphvnqlgceargmgm*mg---

---XXHXXQFVFIHQXXXLQPLQQLHQLSQLQPLQLLQVQQLVELQLVVQLLIVVLLPQP
IVALPPHHHQAVLLQVPLQVNHQVAPLQVVLHPLLQVALHPLSQVVLHHHQLQVQLHHQ

Frame B:
llaywxsfnfviikkesnnyiyi*ei*kk*nexfyi*llfyf*lhyiyhqllvgvvaeiv
kvivqnihvnqievvhiyhlyhvvvrknfsvliviieivvmixhvlktrelvkslrfgqv
vnhieilyhiispkynhm*in*VVKQEVWECEWX---

---xxxsxnlyssixxxcfnhcnncincrnynlcnycrynnwwnynwwfnf***ccflsr
**rcllitikqccfkcrfk*tik*rrfk*csilcfk*rsilclk*csiiisfkfsciis

Frame C:
cwptgihsil*l*krnqiiiyiykkykknkmxnfifsfsfifnyiisitnfwlgwwqrl*
kssfriym*ir*klcifticimlw*eriflc*s*s*kll**ximc*khenw*nl*dlvkl
*tt*rfctisshpntttcesirl*skrygnvng---

---xxxhxicihpsxxxastiattastvatttsattagtttggtttggstsdssaassad
ssaassspsssaassaassepsssaasssapssasssapssvsssapsssasssaass
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH530 (SFH530Q) /CSM/SF/SFH5-B/SFH530Q.Seq.d/                       1324   0.0
SFI362 (SFI362Q) /CSM/SF/SFI3-C/SFI362Q.Seq.d/                        664   0.0
SSA149 (SSA149Q) /CSM/SS/SSA1-C/SSA149Q.Seq.d/                        654   0.0
SLG851 (SLG851Q) /CSM/SL/SLG8-C/SLG851Q.Seq.d/                        654   0.0
SLB538 (SLB538Q) /CSM/SL/SLB5-B/SLB538Q.Seq.d/                        654   0.0
SLA438 (SLA438Q) /CSM/SL/SLA4-B/SLA438Q.Seq.d/                        654   0.0
SLA432 (SLA432Q) /CSM/SL/SLA4-B/SLA432Q.Seq.d/                        654   0.0
SLA402 (SLA402Q) /CSM/SL/SLA4-A/SLA402Q.Seq.d/                        654   0.0
SHL109 (SHL109Q) /CSM/SH/SHL1-A/SHL109Q.Seq.d/                        654   0.0
SHJ527 (SHJ527Q) /CSM/SH/SHJ5-B/SHJ527Q.Seq.d/                        654   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.   636   0.0    7
CF594799|CF594799.1 AGENCOURT_15713545 NIH_ZGC_8 Danio rerio cDNA clone IMAGE:7014266 5', mRNA sequence.    38   0.028  3
AC084865|AC084865.3 Homo sapiens chromosome 7 clone RP11-437L1, complete sequence.    48   0.19   1
AC079746|AC079746.2 Homo sapiens chromosome 7 clone CTD-2353O2, WORKING DRAFT SEQUENCE, 19 unordered pieces.    48   0.19   1
AZ208391|AZ208391.1 SP_0150_A1_B06_T7A Strongylocentrotus purpuratus, purple sea urchin , sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=150 Col=11 Row=C, DNA sequence.    48   0.19   1
AC073320|AC073320.8 Homo sapiens clone RP11-306G20, complete sequence.    48   0.19   1
AC145891|AC145891.1 Pan troglodytes chromosome UNK clone RP43-20A24, *** SEQUENCING IN PROGRESS ***, 63 unordered pieces.    48   0.19   1
BG603433|BG603433.1 EST502523 Plasmodium yoelii sporozoite cDNA Plasmodium yoelii cDNA clone PYCDR54, mRNA sequence.    38   0.44   2
BG603400|BG603400.1 EST502490 Plasmodium yoelii sporozoite cDNA Plasmodium yoelii cDNA clone PYCDR24, mRNA sequence.    38   0.48   2
BX119921|BX119921.9 Zebrafish DNA sequence from clone CH211-198N10 in linkage group 16.    46   0.76   1
dna update 2003.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       201   2e-50
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   198   2e-49
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    79   2e-13
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    54   8e-06
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    46   0.002
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    43   0.014
AM904678_1(AM904678|pid:none) Oncorhynchus mykiss partial mRNA f...    35   2.2
AB255883_1(AB255883|pid:none) Leucophaea maderae VgR mRNA for vi...    35   2.2
(Q9NZR2) RecName: Full=Low-density lipoprotein receptor-related ...    35   3.7
AF283408_1(AF283408|pid:none) Homo sapiens candidate tumor suppr...    35   3.7
protein update 2009. 1.11
PSORT 

 psg: 0.75  gvh: 0.51  alm: 0.23  top: 0.70  tms: 0.07  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.27  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for SFH530 is nuc
5' end seq. ID SFH530F
5' end seq. 
>SFH530F.Seq
ACTGTTGGCCTACTGGNATTCATTCAATTTTGTAATTATAAAAAAGGAATCAAATAATTA
TATATATATATAAGAAATATAAAAAAAATAAAATGAGNAATTTTATATTTAGCTTCTCTT
TTATTTTTAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGT
GAAAGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTT
GTATCATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGT
GATGATTNATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGT
TGTAAACCACATAGAGATTTTGTACCATATCATCTCACCCAAATACAACCACATGTGAAT
CAATTAGGTTGTGAAGCAAGAGGTATGGGAATGTGAATGGGNT----------
Length of 5' end seq. 463
3' end seq. ID SFH530Z
3' end seq. 
>SFH530Z.Seq
NNNNNNNNNNGNTGNNCATNTCATNCAATTTGTATTCATCCATCAANTNNTGNTGCTTCA
ACCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACA
ACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCC
GATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGT
GAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCA
TCCTCTGTCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGC
Length of 3' end seq. 359
Connected seq. ID SFH530P
Connected seq. 
>SFH530P.Seq
ACTGTTGGCCTACTGGNATTCATTCAATTTTGTAATTATAAAAAAGGAATCAAATAATTA
TATATATATATAAGAAATATAAAAAAAATAAAATGAGNAATTTTATATTTAGCTTCTCTT
TTATTTTTAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGT
GAAAGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTT
GTATCATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGT
GATGATTNATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGT
TGTAAACCACATAGAGATTTTGTACCATATCATCTCACCCAAATACAACCACATGTGAAT
CAATTAGGTTGTGAAGCAAGAGGTATGGGAATGTGAATGGGNT----------GNTGNNC
ATNTCATNCAATTTGTATTCATCCATCAANTNNTGNTGCTTCAACCATTGCAACAACTGC
ATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAACTGGTGGAACTAC
AACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCGCTGCCTC
CTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGTAGCGC
CGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTCTGTCTCAAGTAG
TGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGC
Length of connected seq. 812
Full length Seq ID -
Full length Seq. -
Length of full length seq. -