>SFH501 (SFH501Q) /CSM/SF/SFH5-A/SFH501Q.Seq.d/
ACTGTTGGCCCACTGGNTATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCA
AGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACT
TCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATT
TGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTT
ATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATG
GGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTA
GCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTA
CAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCA
GGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATXXXXXXXXXXGGATCCAA
TCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATGTCGCAGAGCGTTTTG
GTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCG
CCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATG
GCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATT
TGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTC
AAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGG
GTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTA
TGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTA
AAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTA
AGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTC
ATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAAGAGAATTACATCGTC
GTAAAGGTAAATATGGTGTAATCTCTATGTGTAT

TVGPLXIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAI
CKAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLL
AGLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWD---

---DPIISGCYNTMGQTSENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVT
VKTEDGKTIVVDKDEGIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRS
TAERLGLKVELVFVAFAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQ
AFYSCKVLGLDMNKVNPNGSGIALGHPLGATGARQMATISRELHRRKGKYGVISMC

Frame A:
TVGPLXIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAI
CKAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLL
AGLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWD---

---gsnh*wllqhygsnl*kcrrafwchqkrpr*ilckftqegrcrpksr*i*r*nrssh
rkdrgwqnhrrr*rrryp*enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekk
yrrkigsqg*igfrsfcccrcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfs
silql*gfgfgyeqs*skwlwyrfrssircywcpsngyhfkritss*r*iwcnlyvy

Frame B:
llahwx*yytp*likchsiqelklflvt*aldqf*nqiqlldqlnqemil*llhhfvqqf
vklkeehskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvqpklelphy*
qvyqrkyhviqstdnvhqvykqlpivqqqskqvsmtlv*qav*nq*v*iqwhg---

---DPIISGCYNTMGQTSENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVT
VKTEDGKTIVVDKDEGIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRS
TAERLGLKVELVFVAFAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQ
AFYSCKVLGLDMNKVNPNGSGIALGHPLGATGARQMATISRELHRRKGKYGVISMC

Frame C:
cwptgynitphn**nviqfkn*nyfsslkhwtnfriksnfwis*irr*ycncctisysnl
*s*krsiqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcnrs*schiis
rftkgstmlysqqtmfirftsncqlcsssqsrfl*hwfsrrcrinefksngmg---

---iqslvvvttlwvkplkmsqsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp
*rprmakpsssiktkvsvrkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kev
ppkdwvsrlnwfs*llllsvsnhplwvlvqplqfqpp*nklvsplkistflksmkllllk
hftvvrfwvwi*tkliqmalvsl*vih*vllvpvkwlpfqenyivvkvnmv*slcv

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH501 (SFH501Q) /CSM/SF/SFH5-A/SFH501Q.Seq.d/                       2381   0.0
VFE239 (VFE239Q) /CSM/VF/VFE2-B/VFE239Q.Seq.d/                       1392   0.0
SFA524 (SFA524Q) /CSM/SF/SFA5-A/SFA524Q.Seq.d/                       1392   0.0
CFF662 (CFF662Q) /CSM/CF/CFF6-C/CFF662Q.Seq.d/                       1392   0.0
SFG475 (SFG475Q) /CSM/SF/SFG4-D/SFG475Q.Seq.d/                       1390   0.0
SFD792 (SFD792Q) /CSM/SF/SFD7-D/SFD792Q.Seq.d/                       1384   0.0
CFC529 (CFC529Q) /CSM/CF/CFC5-B/CFC529Q.Seq.d/                       1384   0.0
CFC445 (CFC445Q) /CSM/CF/CFC4-B/CFC445Q.Seq.d/                       1384   0.0
AFE219 (AFE219Q) /CSM/AF/AFE2-A/AFE219Q.Seq.d/                       1384   0.0
SFG758 (SFG758Q) /CSM/SF/SFG7-C/SFG758Q.Seq.d/                       1380   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1392   0.0    2
CA917334|CA917334.1 EST641481 GPOD Medicago truncatula cDNA clone GPOD-35H18, mRNA sequence.    38   4e-17  7
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60   2e-13  5
CA858588|CA858588.1 EST635843 GLSD Medicago truncatula cDNA clone pGLSD-38M16, mRNA sequence.    38   3e-13  6
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44   1e-12  5
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60   1e-11  4
CF213585|CF213585.1 CGF1000794_F07 Vitis vinifera cv. cabernet sauvignon Stem - CAST Vitis vinifera cDNA clone CAST0006_IF_F07 5', mRNA sequence.    60   6e-10  3
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68   1e-09  3
CD007738|CD007738.1 VVB078G11_334604 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB078G11 5, mRNA sequence.    60   2e-08  2
CA515447|CA515447.1 KS09042A06 KS09 Capsicum annuum cDNA, mRNA sequence.    68   3e-08  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   433   0.0
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   249   e-110
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   249   e-109
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   248   e-109
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   246   e-109
BT070433_1(BT070433|pid:none) Picea sitchensis clone WS02729_F15...   248   e-108
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   254   e-108
BT034496_1(BT034496|pid:none) Zea mays full-length cDNA clone ZM...   254   e-108
(Q56WD9) RecName: Full=3-ketoacyl-CoA thiolase 2, peroxisomal;  ...   251   e-108
T07989(T07989) acetyl-CoA C-acyltransferase (EC 2.3.1.16) precur...   250   e-108

 psg: 0.91  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.30  mip: 0.06
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SFH501 is nuc

>SFH501F.Seq
ACTGTTGGCCCACTGGNTATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCA
AGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACT
TCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATT
TGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTT
ATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATG
GGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTA
GCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTA
CAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCA
GGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGAT----------

>SFH501Z.Seq
NNNNNNNNNNGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAA
ATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCAC
ACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCA
CCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGA
AAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCA
CCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAA
GTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCG
GTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAAC
AAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTC
AAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCT
CTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTT
CAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTATGTGTAT

>SFH501P.Seq
ACTGTTGGCCCACTGGNTATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCA
AGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACT
TCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATT
TGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTT
ATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATG
GGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTA
GCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTA
CAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCA
GGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGAT----------GGATCCAA
TCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATGTCGCAGAGCGTTTTG
GTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCG
CCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATG
GCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATT
TGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTC
AAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGG
GTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTA
TGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTA
AAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTA
AGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTC
ATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAAGAGAATTACATCGTC
GTAAAGGTAAATATGGTGTAATCTCTATGTGTAT