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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFH270
Library SF
(Link to library)
Clone ID SFH270
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10672-1
Original site URL
Representative seq. ID SFH270P
(Link to Original site)
Representative DNA sequence 
>SFH270 (SFH270Q) /CSM/SF/SFH2-C/SFH270Q.Seq.d/
ATTATTAGTTAATTTTCATTTGTAAATATAAAAAAAAAATGGAATCATTAAAAAACAATG
GTGAAAAAATTGAAAATTTTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAG
GTTTTAAAGAATTTTCAAATTCTCCAAATACTTTAAATGCTGAAAAAGATGGAGAGTTTC
TTGATAAACATGATGAAGAATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATA
AAATTTCAAATTTAAGTTCTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTT
TTCAAACAATTCTATACAATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATA
TTATGGGTAATGGTATAAATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAAC
AAATGATTAAAAAATCATTAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAA
ATTTAGGAAAAGCATGTGATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATG
GTATCAAATCAATGAACCXXXXXXXXXXTGTTAATGTAACATTATCTGAATTTTTAATAT
CAGGTCGTGAATACACCAATGTTGTTAATTCTCTTCAAGAAGGTTATTGGATAACATATC
CACCACCAATTTATAAGGTTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTG
CCTCGGAATTGGCACATAAATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTG
ATCTAATTTATATTAAAAAAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCATG
CCCTTGAAGTTTATTATAGCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTC
GTTATGGTGGAAAACCAATCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAA
TGTTAAATCCAAATCGTTATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTA
GAGAAGCCAAATGGAATTTAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTA
ACCAAAATTTTGTTTTCACTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATATTG
GAACTGTTGGTATCACTGAAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAGAT
TAACATGCATTCGTAAAGTAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTGGATC
TCGATTTATCAGTACAATAATNATTANAACCACT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
y*LIFICKYKKKMESLKNNGEKIENFLNQLKNLQSNVDKGFKEFSNSPNTLNAEKDGEFL
DKHDEELYNKQIIRSRLEDKISNLSSSMINDIGNDFQTGFQTILYNLPGVGPVFQMIFNI
MGNGINLEKFYQEIITQVEQMIKKSLEDYYKNQCNTIFKNLGKACDQHKDLTQKWYDKNG
IKSMN---

---VNVTLSEFLISGREYTNVVNSLQEGYWITYPPPIYKVWVPPQQASWVSPPASELAHK
FFLAHSDNYPQGGDLIYIKKDGVYELSPNKITHALEVYYSSGGGYGVNDYPSFRYGGKPI
IPLLAPVGNAVFTMLNPNRYKRTFKIRIVHVIVREAKWNLDCYDNQVGEAGSFNQNFVFT
STNIVSDPICGNIGTVGITEIPGPFTTDKKYIRLTCIRKVNFPASKNEGYAVDLDLSVQ*
xlxp
Translated Amino Acid sequence (All Frames) 
Frame A:
iis*fsfvnikkkwnh*ktmvkklkif*in*kifnqm*tkvlknfqilqil*mlkkmesf
linmmknyttnk*fvqd*kikfqi*vll*lmilgmifklvfkqfytiyqvlvqfsk*sli
lwvmv*i*knftkkslhk*nk*lknh*kiiiktnviqflki*ekhviniki*lkngmikm
vsnq*t---

---c*cnii*ifnirs*ihqcc*fssrrlldnisttnl*glgstttsivgissclgigt*
ilfgpf*qlssrw*snly*krwsl*t*sk*nnscp*sll*lwgwiwc**lskfslwwktn
htssrssw*ccvynvksksl*knl*n*ncschc*rsqmefrll**sswgsrfi*pkfcfh
fn*ysf*pnlweywncwyh*nsrtiyn**kvy*inmhs*skfpsikk*ricrgsrfisti
xixtt

Frame B:
llvnfhl*i*kkngiikkqw*kn*kffksikkssikcrqrf*rifkfskyfkc*krwrvs
**t**riiqqtnnsfktrr*nfkfkffyd**ywe*fsnwfsnnsiqftrcwssfpndl*y
yg*wykfrkilprnhytsrtnd*kiirrll*kpm*ynf*kfrksm*st*rsnskmv**kw
yqine---

---VNVTLSEFLISGREYTNVVNSLQEGYWITYPPPIYKVWVPPQQASWVSPPASELAHK
FFLAHSDNYPQGGDLIYIKKDGVYELSPNKITHALEVYYSSGGGYGVNDYPSFRYGGKPI
IPLLAPVGNAVFTMLNPNRYKRTFKIRIVHVIVREAKWNLDCYDNQVGEAGSFNQNFVFT
STNIVSDPICGNIGTVGITEIPGPFTTDKKYIRLTCIRKVNFPASKNEGYAVDLDLSVQ*
xlxp

Frame C:
y*LIFICKYKKKMESLKNNGEKIENFLNQLKNLQSNVDKGFKEFSNSPNTLNAEKDGEFL
DKHDEELYNKQIIRSRLEDKISNLSSSMINDIGNDFQTGFQTILYNLPGVGPVFQMIFNI
MGNGINLEKFYQEIITQVEQMIKKSLEDYYKNQCNTIFKNLGKACDQHKDLTQKWYDKNG
IKSMN---

---lm*hylnf*yqvvntpmllilfkkvig*hihhqfirfgfhhnkhrgylllprnwhin
sfwpiltiilkvvi*filkkmefmnlvqik*lmplkfiialgvdmvlmtiqvfvmvenqs
ylfslqlvmlclqc*iqivikeplklelfmsllekpngi*iamiiklgkqvhltkilfsl
qli*fltqfvgilellvslkfqdhlqliksild*hafvk*isqhqkmkdmpwisiyqynn
xxnh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH270 (SFH270Q) /CSM/SF/SFH2-C/SFH270Q.Seq.d/                       2276   0.0
AHH207 (AHH207Q) /CSM/AH/AHH2-A/AHH207Q.Seq.d/                       1368   0.0
AFG364 (AFG364Q) /CSM/AF/AFG3-C/AFG364Q.Seq.d/                       1368   0.0
AFC537 (AFC537Q) /CSM/AF/AFC5-B/AFC537Q.Seq.d/                       1368   0.0
CFC405 (CFC405Q) /CSM/CF/CFC4-A/CFC405Q.Seq.d/                       1362   0.0
AFH880 (AFH880Q) /CSM/AF/AFH8-D/AFH880Q.Seq.d/                       1362   0.0
AFI828 (AFI828Q) /CSM/AF/AFI8-B/AFI828Q.Seq.d/                       1358   0.0
AHB192 (AHB192Q) /CSM/AH/AHB1-D/AHB192Q.Seq.d/                       1356   0.0
CFB708 (CFB708Q) /CSM/CF/CFB7-A/CFB708Q.Seq.d/                       1342   0.0
AFK369 (AFK369Q) /CSM/AF/AFK3-C/AFK369Q.Seq.d/                       1340   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116920|AC116920.2 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strainAX4, complete sequence.  1150   0.0    12
M15968|M15968.1 D.discoideum M3R gene, exons 1-3.                 541   0.0    3
M15967|M15967.1 D.discoideum M3L gene, exons 1-3.                 230   e-141  3
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    34   9e-09  20
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34   1e-07  22
AF303741|AF303741.1 Chilo iridescent virus complete genome.        32   1e-06  17
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    34   3e-06  19
AL445563|AL445563.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 1/3.    32   1e-05  19
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    34   1e-05  19
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    30   5e-05  19
dna update 2003.12.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P11872) RecName: Full=cAMP-regulated M3R protein; AltName: Full...   459   e-127
AC117072_17(AC117072|pid:none) Dictyostelium discoideum chromoso...   451   e-125
AE014187_536(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    45   0.007
AE014186_384(AE014186|pid:none) Plasmodium falciparum 3D7 chromo...    44   0.016
CR380952_40(CR380952|pid:none) Candida glabrata strain CBS138 ch...    43   0.021
CP001357_1913(CP001357|pid:none) Brachyspira hyodysenteriae WA1,...    40   0.18
(O51267) RecName: Full=Leucyl-tRNA synthetase;          EC=6.1.1...    40   0.23
CP001205_240(CP001205|pid:none) Borrelia burgdorferi ZS7, comple...    40   0.23
CP000879_149(CP000879|pid:none) Petrotoga mobilis SJ95, complete...    39   0.30
AL844507_2(AL844507|pid:none) Plasmodium falciparum 3D7 chromoso...    39   0.30
protein update 2009. 5.25
PSORT 

 psg: 0.56  gvh: 0.29  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for SFH270 is cyt
5' end seq. ID SFH270F
5' end seq. 
>SFH270F.Seq
ATTATTAGTTAATTTTCATTTGTAAATATAAAAAAAAAATGGAATCATTAAAAAACAATG
GTGAAAAAATTGAAAATTTTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAG
GTTTTAAAGAATTTTCAAATTCTCCAAATACTTTAAATGCTGAAAAAGATGGAGAGTTTC
TTGATAAACATGATGAAGAATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATA
AAATTTCAAATTTAAGTTCTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTT
TTCAAACAATTCTATACAATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATA
TTATGGGTAATGGTATAAATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAAC
AAATGATTAAAAAATCATTAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAA
ATTTAGGAAAAGCATGTGATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATG
GTATCAAATCAATGAACC----------
Length of 5' end seq. 558
3' end seq. ID SFH270Z
3' end seq. 
>SFH270Z.Seq
----------TGTTAATGTAACATTATCTGAATTTTTAATATCAGGTCGTGAATACACCA
ATGTTGTTAATTCTCTTCAAGAAGGTTATTGGATAACATATCCACCACCAATTTATAAGG
TTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTGCCTCGGAATTGGCACATA
AATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTGATCTAATTTATATTAAAA
AAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCATGCCCTTGAAGTTTATTATA
GCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTCGTTATGGTGGAAAACCAA
TCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAATGTTAAATCCAAATCGTT
ATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTAGAGAAGCCAAATGGAATT
TAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTAACCAAAATTTTGTTTTCA
CTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATATTGGAACTGTTGGTATCACTG
AAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAGATTAACATGCATTCGTAAAG
TAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTGGATCTCGATTTATCAGTACAAT
AATNATTANAACCACT
Length of 3' end seq. 726
Connected seq. ID SFH270P
Connected seq. 
>SFH270P.Seq
ATTATTAGTTAATTTTCATTTGTAAATATAAAAAAAAAATGGAATCATTAAAAAACAATG
GTGAAAAAATTGAAAATTTTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAG
GTTTTAAAGAATTTTCAAATTCTCCAAATACTTTAAATGCTGAAAAAGATGGAGAGTTTC
TTGATAAACATGATGAAGAATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATA
AAATTTCAAATTTAAGTTCTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTT
TTCAAACAATTCTATACAATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATA
TTATGGGTAATGGTATAAATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAAC
AAATGATTAAAAAATCATTAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAA
ATTTAGGAAAAGCATGTGATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATG
GTATCAAATCAATGAACC----------TGTTAATGTAACATTATCTGAATTTTTAATAT
CAGGTCGTGAATACACCAATGTTGTTAATTCTCTTCAAGAAGGTTATTGGATAACATATC
CACCACCAATTTATAAGGTTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTG
CCTCGGAATTGGCACATAAATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTG
ATCTAATTTATATTAAAAAAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCATG
CCCTTGAAGTTTATTATAGCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTC
GTTATGGTGGAAAACCAATCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAA
TGTTAAATCCAAATCGTTATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTA
GAGAAGCCAAATGGAATTTAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTA
ACCAAAATTTTGTTTTCACTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATATTG
GAACTGTTGGTATCACTGAAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAGAT
TAACATGCATTCGTAAAGTAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTGGATC
TCGATTTATCAGTACAATAATNATTANAACCACT
Length of connected seq. 1284
Full length Seq ID -
Full length Seq. -
Length of full length seq. -