>SFH223 (SFH223Q) /CSM/SF/SFH2-A/SFH223Q.Seq.d/
ACTTTTTTATTTTTAAAATTAAGGATGCATAAAATTGTTCAACAAGGTAGTAAATCATTA
ATTGAATCAAGAATTAAAAGAAAATCATTATTAAGTAAAGTTGTTACAGATGTTAGTCAA
TTGATACCATATTTTCAAAATGGACATTATGTGTCTATGGGTGGATTTGCAGGTACAGGT
TATCCAAAGGTAGTACCAATTGCATTGGCAGACCATGTGGAAAAAAATGGATTACAGGGT
AAATTAAAAATGAATTTATTTGTTGGAGCATCAGTTGGACCAGAGACAGAAGATCGTTGG
GCAATGTTGGATATGATAGATAAGAGATATCCTCACCAAAATGGACATCACATTCGTAAT
GGTATAAATAGCGGTAGAATTCGTTTTGCCGATCAACATTTATCGACATTTGCAAGTGAT
TTATTGGCTGGATATTATACATTGATAAACCACATGGATCAAAGAGAACAATGGATATAG
CAATCGTTGAAGCCACAGAAATCACCGAAGACGGTTGTATAGTACCAGGTGCATCCGTTG
GTATCACACCTGAGATCTTACAAATGGCAGATAAAATCATCATTGAGATTAACACCTCAT
TACCATCGTTCAAAGGGTTGCATGATATGGTGAAAATTGCATTACCTXXXXXXXXXXTGG
TTCAATTGCAAACGCTGTCATTGNAGGTCTTTCCAAAGGTCCATTTGACAATCTCTCAGT
TTGGACTGAAGTTATTCAAGACACTTTCTTGGACTTTTTCGATAATGGTAAATTGAAATT
CGCTTCAGCAACTTCACTTAGATTCTCCCCACCAGGTTTCAATAGATTATTCAATAATTG
GGAAAATTATAAAAGTAAAATCATCCTTAGAAATCAGGCCATTTCAAATGCAGCTGAATT
AATTTCACGTGTTGGTTGTATCGCTTTAAATACTCCATGTGAAGTTGATATCTATGGTCA
TGTCAATAGTACCAATACAATGGGTTCAAAAATGTTAAATGGTTTAGGTGGTAGTGGTGA
ATTCTTAAGAAATTCAAGAATCTCCATTGTTCACACCCCATCAACTCGTCCAACTAAAAC
TGATCCACATGGTATTAGTTGTATCGTTCCTTTCGCCTCTCATATCGATCATACAGAACA
CGACATTGATATCATTGTAACCGAGCAAGGTTTAGCTGATATTCGTGGTTTGGCACCTTA
TGAACGTGCTAAAGTTATCATTCAAAATTGTGCTCATCCAATCTATAAACCAATACTCAT
GGAATACCTTGAAACTTCTCGTCAAATTTGTTTAAAAAATCATATGGGACATGAACCACA
TCAATTAGACAAAGCATTTAAATTTTATACAAATTATCNAAAAAGGACTATAAAATNA

ffifkikda*ncstr**iin*ikn*kkiiik*scyrc*sidtifskwtlcvygwicryrl
skgstncigrpcgkkwitg*ikneficwsiswtrdrrslgnvgydr*eisspkwtshs*w
yk*r*nsfcrstfidick*FIGWILYIDKPHGSKRTMDIAIVEATEITEDGCIVPGASVG
ITPEILQMADKIIIEINTSLPSFKGLHDMVKIALP---

---GSIANAVIXGLSKGPFDNLSVWTEVIQDTFLDFFDNGKLKFASATSLRFSPPGFNRL
FNNWENYKSKIILRNQAISNAAELISRVGCIALNTPCEVDIYGHVNSTNTMGSKMLNGLG
GSGEFLRNSRISIVHTPSTRPTKTDPHGISCIVPFASHIDHTEHDIDIIVTEQGLADIRG
LAPYERAKVIIQNCAHPIYKPILMEYLETSRQICLKNHMGHEPHQLDKAFKFYTNYXKRT
IKX

Frame A:
tflflklrmhkivqqgsksliesrikrksllskvvtdvsqlipyfqnghyvsmggfagtg
ypkvvpialadhveknglqgklkmnlfvgasvgpetedrwamldmidkryphqnghhirn
ginsgrirfadqhlstfasdllagyytlinhmdqreqwi*qslkpqkspktvv*yqvhpl
vshlrsykwqiksslrltphyhrskgcmiw*klhy---

---wfnckrchxrsfqrsi*qslsld*sysrhflglfr*w*ieirfsnft*ilptrfq*i
iq*lgkl*k*nhp*ksghfkcs*inftcwlyrfkysm*s*ylwscq*yqyngfknvkwfr
w*w*ilkkfknlhcshpinssn*n*stwy*lyrsfrlsyrsyrtrh*yhcnrarfs*ysw
fgtl*tc*syhsklcssnl*tnthgip*nfssnlfkksygt*ttsirqsi*ilyklskkd
ykx

Frame B:
lfyf*n*gciklfnkvvnh*lnqelkenhy*vkllqmlvn*yhifkmdimclwvdlqvqv
iqr*yqlhwqtmwkkmdyrvn*k*iyllehqldqrqkivgqcwi**irdiltkmditfvm
v*iavefvlpiniyrhlqviywldiih**ttwikenngysnr*shrnhrrrlystrcirw
yht*dltngr*nhh*d*hlitivqrva*ygencit---

---GSIANAVIXGLSKGPFDNLSVWTEVIQDTFLDFFDNGKLKFASATSLRFSPPGFNRL
FNNWENYKSKIILRNQAISNAAELISRVGCIALNTPCEVDIYGHVNSTNTMGSKMLNGLG
GSGEFLRNSRISIVHTPSTRPTKTDPHGISCIVPFASHIDHTEHDIDIIVTEQGLADIRG
LAPYERAKVIIQNCAHPIYKPILMEYLETSRQICLKNHMGHEPHQLDKAFKFYTNYXKRT
IKX

Frame C:
ffifkikda*ncstr**iin*ikn*kkiiik*scyrc*sidtifskwtlcvygwicryrl
skgstncigrpcgkkwitg*ikneficwsiswtrdrrslgnvgydr*eisspkwtshs*w
yk*r*nsfcrstfidick*FIGWILYIDKPHGSKRTMDIAIVEATEITEDGCIVPGASVG
ITPEILQMADKIIIEINTSLPSFKGLHDMVKIALP---

---vqlqtlslxvfpkvhltisqfglklfktlswtfsimvn*nslqqlhldsphqvsidy
siigkiikvkssleirpfqmqln*fhvlvvsl*ilhvklismvmsivpiqwvqkc*mv*v
vvvns*eiqesplftphqlvqlklihmvlvvsflsplisiiqnttlisl*pskv*lifvv
whlmnvlklsfkivliqsinqyswntlkllvkfv*kiiwdmnhin*tkhlnfiqiixkgl
*n

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH223 (SFH223Q) /CSM/SF/SFH2-A/SFH223Q.Seq.d/                       2587   0.0
AFD589 (AFD589Q) /CSM/AF/AFD5-D/AFD589Q.Seq.d/                       1398   0.0
AFD648 (AFD648Q) /CSM/AF/AFD6-B/AFD648Q.Seq.d/                       1392   0.0
AFO437 (AFO437Q) /CSM/AF/AFO4-B/AFO437Q.Seq.d/                       1390   0.0
CFJ115 (CFJ115Q) /CSM/CF/CFJ1-A/CFJ115Q.Seq.d/                       1382   0.0
SFK723 (SFK723Q) /CSM/SF/SFK7-A/SFK723Q.Seq.d/                       1380   0.0
CFK206 (CFK206Q) /CSM/CF/CFK2-A/CFK206Q.Seq.d/                       1380   0.0
CFK122 (CFK122Q) /CSM/CF/CFK1-A/CFK122Q.Seq.d/                       1380   0.0
AFH124 (AFH124Q) /CSM/AF/AFH1-A/AFH124Q.Seq.d/                       1380   0.0
FC-AL01 (FC-AL01Q) /CSM/FC/FC-AL/FC-AL01Q.Seq.d/                     1378   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL409684|AL409684.1 T7 end of clone XAV0AA001D05 of library XAV0AA from strain CBS 379 of Saccharomyces exiguus.    62   1e-10  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34   4e-06  21
AL139189|AL139189.14 Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP11-94F16.    34   4e-04  9
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    38   0.004  12
AR319568|AR319568.1 Sequence 2118 from patent US 6562958.          40   0.014  2
AC122580|AC122580.5 Rattus norvegicus clone CH230-29H2, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    42   0.019  7
AC005506|AC005506.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    36   0.043  10
AL929357|AL929357.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 3/5.    34   0.14   14
AC073928|AC073928.5 Homo sapiens chromosome 2 clone RP11-745P9, complete sequence.    34   0.20   8
AE017033|AE017033.1 Bacillus anthracis str. Ames section 10 of 18 of the complete genome.    32   0.23   14

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54K91) RecName: Full=Acetyl-CoA hydrolase;          EC=3.1.2.1...   446   e-123
AM920427_313(AM920427|pid:none) Penicillium chrysogenum Wisconsi...   306   1e-81
AP007151_504(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   304   4e-81
A36316(A36316) acu-8 protein - Neurospora crassa  &M31521_1(M315...   300   6e-80
CU633453_78(CU633453|pid:none) Podospora anserina genomic DNA ch...   298   4e-79
D89222_1(D89222|pid:none) Schizosaccharomyces pombe mRNA, partia...   288   2e-76
(Q9UUJ9) RecName: Full=Acetyl-CoA hydrolase;          EC=3.1.2.1...   288   3e-76
AE017180_488(AE017180|pid:none) Geobacter sulfurreducens PCA, co...   284   4e-75
CP000148_1711(CP000148|pid:none) Geobacter metallireducens GS-15...   284   4e-75
CP001390_726(CP001390|pid:none) Geobacter sp. FRC-32, complete g...   284   6e-75

 psg: 0.57  gvh: 0.32  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SFH223 is cyt

>SFH223F.Seq
ACTTTTTTATTTTTAAAATTAAGGATGCATAAAATTGTTCAACAAGGTAGTAAATCATTA
ATTGAATCAAGAATTAAAAGAAAATCATTATTAAGTAAAGTTGTTACAGATGTTAGTCAA
TTGATACCATATTTTCAAAATGGACATTATGTGTCTATGGGTGGATTTGCAGGTACAGGT
TATCCAAAGGTAGTACCAATTGCATTGGCAGACCATGTGGAAAAAAATGGATTACAGGGT
AAATTAAAAATGAATTTATTTGTTGGAGCATCAGTTGGACCAGAGACAGAAGATCGTTGG
GCAATGTTGGATATGATAGATAAGAGATATCCTCACCAAAATGGACATCACATTCGTAAT
GGTATAAATAGCGGTAGAATTCGTTTTGCCGATCAACATTTATCGACATTTGCAAGTGAT
TTATTGGCTGGATATTATACATTGATAAACCACATGGATCAAAGAGAACAATGGATATAG
CAATCGTTGAAGCCACAGAAATCACCGAAGACGGTTGTATAGTACCAGGTGCATCCGTTG
GTATCACACCTGAGATCTTACAAATGGCAGATAAAATCATCATTGAGATTAACACCTCAT
TACCATCGTTCAAAGGGTTGCATGATATGGTGAAAATTGCATTACCT----------

>SFH223Z.Seq
----------TGGTTCAATTGCAAACGCTGTCATTGNAGGTCTTTCCAAAGGTCCATTTG
ACAATCTCTCAGTTTGGACTGAAGTTATTCAAGACACTTTCTTGGACTTTTTCGATAATG
GTAAATTGAAATTCGCTTCAGCAACTTCACTTAGATTCTCCCCACCAGGTTTCAATAGAT
TATTCAATAATTGGGAAAATTATAAAAGTAAAATCATCCTTAGAAATCAGGCCATTTCAA
ATGCAGCTGAATTAATTTCACGTGTTGGTTGTATCGCTTTAAATACTCCATGTGAAGTTG
ATATCTATGGTCATGTCAATAGTACCAATACAATGGGTTCAAAAATGTTAAATGGTTTAG
GTGGTAGTGGTGAATTCTTAAGAAATTCAAGAATCTCCATTGTTCACACCCCATCAACTC
GTCCAACTAAAACTGATCCACATGGTATTAGTTGTATCGTTCCTTTCGCCTCTCATATCG
ATCATACAGAACACGACATTGATATCATTGTAACCGAGCAAGGTTTAGCTGATATTCGTG
GTTTGGCACCTTATGAACGTGCTAAAGTTATCATTCAAAATTGTGCTCATCCAATCTATA
AACCAATACTCATGGAATACCTTGAAACTTCTCGTCAAATTTGTTTAAAAAATCATATGG
GACATGAACCACATCAATTAGACAAAGCATTTAAATTTTATACAAATTATCNAAAAAGGA
CTATAAAATNA

>SFH223P.Seq
ACTTTTTTATTTTTAAAATTAAGGATGCATAAAATTGTTCAACAAGGTAGTAAATCATTA
ATTGAATCAAGAATTAAAAGAAAATCATTATTAAGTAAAGTTGTTACAGATGTTAGTCAA
TTGATACCATATTTTCAAAATGGACATTATGTGTCTATGGGTGGATTTGCAGGTACAGGT
TATCCAAAGGTAGTACCAATTGCATTGGCAGACCATGTGGAAAAAAATGGATTACAGGGT
AAATTAAAAATGAATTTATTTGTTGGAGCATCAGTTGGACCAGAGACAGAAGATCGTTGG
GCAATGTTGGATATGATAGATAAGAGATATCCTCACCAAAATGGACATCACATTCGTAAT
GGTATAAATAGCGGTAGAATTCGTTTTGCCGATCAACATTTATCGACATTTGCAAGTGAT
TTATTGGCTGGATATTATACATTGATAAACCACATGGATCAAAGAGAACAATGGATATAG
CAATCGTTGAAGCCACAGAAATCACCGAAGACGGTTGTATAGTACCAGGTGCATCCGTTG
GTATCACACCTGAGATCTTACAAATGGCAGATAAAATCATCATTGAGATTAACACCTCAT
TACCATCGTTCAAAGGGTTGCATGATATGGTGAAAATTGCATTACCT----------TGG
TTCAATTGCAAACGCTGTCATTGNAGGTCTTTCCAAAGGTCCATTTGACAATCTCTCAGT
TTGGACTGAAGTTATTCAAGACACTTTCTTGGACTTTTTCGATAATGGTAAATTGAAATT
CGCTTCAGCAACTTCACTTAGATTCTCCCCACCAGGTTTCAATAGATTATTCAATAATTG
GGAAAATTATAAAAGTAAAATCATCCTTAGAAATCAGGCCATTTCAAATGCAGCTGAATT
AATTTCACGTGTTGGTTGTATCGCTTTAAATACTCCATGTGAAGTTGATATCTATGGTCA
TGTCAATAGTACCAATACAATGGGTTCAAAAATGTTAAATGGTTTAGGTGGTAGTGGTGA
ATTCTTAAGAAATTCAAGAATCTCCATTGTTCACACCCCATCAACTCGTCCAACTAAAAC
TGATCCACATGGTATTAGTTGTATCGTTCCTTTCGCCTCTCATATCGATCATACAGAACA
CGACATTGATATCATTGTAACCGAGCAAGGTTTAGCTGATATTCGTGGTTTGGCACCTTA
TGAACGTGCTAAAGTTATCATTCAAAATTGTGCTCATCCAATCTATAAACCAATACTCAT
GGAATACCTTGAAACTTCTCGTCAAATTTGTTTAAAAAATCATATGGGACATGAACCACA
TCAATTAGACAAAGCATTTAAATTTTATACAAATTATCNAAAAAGGACTATAAAATNA