SFH221 [Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview]

SFH221
Library	SF (Link to library)
Clone ID	SFH221 (Link to dictyBase)
Atlas ID	-
NBRP ID	-
dictyBase ID	-
Link to Contig	Contig-U14949-1
Original site URL	http://dictycdb.biol.tsukuba.ac.jp/CSM/SF/SFH2-A/SFH221Q.Seq.d/
Representative seq. ID	SFH221P (Link to Original site)
Representative DNA sequence	>SFH221 (SFH221Q) /CSM/SF/SFH2-A/SFH221Q.Seq.d/ ATAAAAATGATTTCATTAGAAGATCTATCACCAACATTATGGAAATCACCAGATAAAGAG GGTGAATTAAAGAAACAAGGACATGTAGTAAAGAATTGGAAGAAAAGAAAGTTTATTATT CAAAATGATATGTTATTTTATTTTAAGGATAAAGAGGAGAGACCAGTTGGAGCGGTGCCA TTAAGAATGTCACGTTGCTATGAAAATAAATCATTAGGTAAACCAAATTGTTTCGAATTA GTATCACCAAGAATCAATAAAACATTTTTCATTCAAGCCAATACACCAGATGAAATGGCA AGTTGGATGAAAGCAGTTGAGAAAGGATCAGAATATAGCACAGTATCACAACCATTCAAT CTTAAACATGAGGTACATGTTGATTTCAATTCAGCAACTGGTTTCTCTGGTTTACCAAAA GAATGGGAAGTTATCTTAAAGTCTTCAAATGTTTCAAAACAAGAAGTACTCGATAAACCA AGTGAATGGTTATCAGTTTTAGAATTACAAGCTGGTAGAACAATGGAAAAATCAAATTCT CAAAATTTATCTGCACTTCCTGATGAATCAAATTTAACTCTTAGTGATTTAGTAAXXXXX XXXXXATGATAATGCCAAACTCTTGGTAACAGAGATTGCAATTATGAAGACATCACATCA TGATAACATTGTAAATTACATTGATAGTTATATAGTGAACGATAGAGAACTTTGGGTTGC AATGGAGTTTATGGGTGGTGGTTGTTTAACAGACATTTTAGAGGCATTTGATAATATCAA AATGAGTGAGATTCAAATTGCTTATGTGGTTAAAGAGACCTTGAAGGCATTGCAATACAT TCATAGTCTTCATCGTATTCATAGAGATATAAAGAGTGATAATATTTTATTGGGCTCAGA GGGTAGTGTAAAGATTGCTGATTTTGGTTATGCCGCTCAATTAACTCAAAAACAACAAAA ACGTAATACCGTCGTTGGTACCCCTTATTGGATGGCCCCAGAACTCATTAGAGGTCACGA TTATGGTGTCAAGGTTGATATTTGGTCTTTGGGTATCATGATGATGGAAATGGCTGAAGG TGAACCACCTTATATGGATTTCCCACCATTGCGTGCTCTTTTCCTCATCACTACTAGAGG TATTCCACCACTTAAAGAAACAACCAAATGGAGTAAAACTTTCCAAGATTTCTTCTCAAA ATGTCTCGATATCAATGTTGCTAATCGTCCTGATGCTACCGATCTTTTAAAACATCCTTT CATGGATTGGCATGNATTCCTCNAATTTAACCATNATTCAAGCCGCAAGAAA
sequence update	2001. 6. 1
Translated Amino Acid sequence	IKMISLEDLSPTLWKSPDKEGELKKQGHVVKNWKKRKFIIQNDMLFYFKDKEERPVGAVP LRMSRCYENKSLGKPNCFELVSPRINKTFFIQANTPDEMASWMKAVEKGSEYSTVSQPFN LKHEVHVDFNSATGFSGLPKEWEVILKSSNVSKQEVLDKPSEWLSVLELQAGRTMEKSNS QNLSALPDESNLTLSDLV--- ---DNAKLLVTEIAIMKTSHHDNIVNYIDSYIVNDRELWVAMEFMGGGCLTDILEAFDNI KMSEIQIAYVVKETLKALQYIHSLHRIHRDIKSDNILLGSEGSVKIADFGYAAQLTQKQQ KRNTVVGTPYWMAPELIRGHDYGVKVDIWSLGIMMMEMAEGEPPYMDFPPLRALFLITTR GIPPLKETTKWSKTFQDFFSKCLDINVANRPDATDLLKHPFMDWHXFLXFNHXSSRKK
Translated Amino Acid sequence (All Frames)	Frame A: IKMISLEDLSPTLWKSPDKEGELKKQGHVVKNWKKRKFIIQNDMLFYFKDKEERPVGAVP LRMSRCYENKSLGKPNCFELVSPRINKTFFIQANTPDEMASWMKAVEKGSEYSTVSQPFN LKHEVHVDFNSATGFSGLPKEWEVILKSSNVSKQEVLDKPSEWLSVLELQAGRTMEKSNS QNLSALPDESNLTLSDLV--- ---mimpnswqrlqlrhhimitlitlivitienfglqwslwvvvvqtfrhlii skvrfkllmwlkrprhcntfivfivfieirviifywaqrvvrllilvmplnlknn knvipslvpligwpqnslevtimvsrlifglwvs*wkwlkvnhliwishhcvlfsssll evfhhlkkqpngvklskissqnvsismllivlmlpifnilswigmxssnltxiqaar Frame B: kfhkiyhqhygnhqikrvnrnkdm*rigrkesllfkmicyfilrikrrdqlerch echvamkinhvnqivsnyhqesikhfsfkpihqmkwqvgkqlrkdqniaqyhnhsi lnmrymlisiqqlvslvyqkngklsslqmfqnkkysinqvngyqfnyklveqwknqil kiylhflmnqillvi--- ---cqtlgnrdcnyeditshcklhlyserrtlgcngvygwwlfnrhfrgiy qnedsnclcgrdlegiaihssssysrykeyfiglrgckdcfwlcrsinsktt ktyrrwyplldgprthrsrlwcqgylvfgyhddgngrttlygfptiacsfphhy* rysttrnnqmenfprfllkmsryqccsscyrsfktsfhglaxipxipxfkpqe Frame C: kndfirrsitnimeitrrgiketrtcskeleekkvyyskyvilfgrgetswsgai knvtllkiirtklfrisitknqnifhssqytrngkldesseririhsittiqs tgtcfqfsnwflwftkrmgsylkvfkcfktrstrtkmvisfritswnngkikfs kfictsikfns*fs--- ---DNAKLLVTEIAIMKTSHHDNIVNYIDSYIVNDRELWVAMEFMGGGCLTDILEAFDNI KMSEIQIAYVVKETLKALQYIHSLHRIHRDIKSDNILLGSEGSVKIADFGYAAQLTQKQQ KRNTVVGTPYWMAPELIRGHDYGVKVDIWSLGIMMMEMAEGEPPYMDFPPLRALFLITTR GIPPLKETTKWSKTFQDFFSKCLDINVANRPDATDLLKHPFMDWHXFLXFNHXSSRKK
Homology vs CSM-cDNA	Score E Sequences producing significant alignments: (bits) Value SFH221 (SFH221Q) /CSM/SF/SFH2-A/SFH221Q.Seq.d/ 2524 0.0 CFH857 (CFH857Q) /CSM/CF/CFH8-C/CFH857Q.Seq.d/ 1368 0.0 AFB708 (AFB708Q) /CSM/AF/AFB7-A/AFB708Q.Seq.d/ 1362 0.0 SFC672 (SFC672Q) /CSM/SF/SFC6-C/SFC672Q.Seq.d/ 1330 0.0 AFK585 (AFK585Q) /CSM/AF/AFK5-D/AFK585Q.Seq.d/ 1310 0.0 VFG871 (VFG871Q) /CSM/VF/VFG8-C/VFG871Q.Seq.d/ 1306 0.0 VFF415 (VFF415Q) /CSM/VF/VFF4-A/VFF415Q.Seq.d/ 1304 0.0 VFE896 (VFE896Q) /CSM/VF/VFE8-D/VFE896Q.Seq.d/ 1300 0.0 SFD370 (SFD370Q) /CSM/SF/SFD3-C/SFD370Q.Seq.d/ 1300 0.0 CFA281 (CFA281Q) /CSM/CF/CFA2-D/CFA281Q.Seq.d/ 1298 0.0
own update	2004.12.25
Homology vs DNA	Score E Sequences producing significant alignments: (bits) Value N AF277804\|AF277804.2 Dictyostelium discoideum PakB mRNA, complete cds. 1009 0.0 4 U67716\|U67716.1 Dictyostelium discoideum myosin I heavy chain kinase (mihck) mRNA, complete cds. 94 3e-49 6 Y10158\|Y10158.1 D.discoideum mRNA for STE20/pak kinase homologue. 94 4e-49 6 AC115606\|AC115606.2 Dictyostelium discoideum chromosome 2 map 159760-209066 strain AX4, complete sequence. 94 6e-44 9 AF131221\|AF131221.1 Dictyostelium discoideum p21-activated protein kinase PakA (pakA) mRNA, complete cds. 66 5e-28 5 U51923\|U51923.3 Dictyostelium discoideum STE20-telated kinase DPAK1 (dpak1) mRNA, complete cds. 60 3e-26 5 AZ677365\|AZ677365.1 ENTFX50TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence. 48 1e-10 5 AZ684578\|AZ684578.1 ENTJV90TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence. 48 1e-10 5 AZ682486\|AZ682486.1 ENTKL40TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence. 42 5e-07 3 AZ682245\|AZ682245.1 ENTMN25TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence. 42 5e-07 3
dna update	2003.12.29
Homology vs Protein	Score E Sequences producing significant alignments: (bits) Value AF277804_1(AF277804\|pid:none) Dictyostelium discoideum PakC mRNA... 450 e-125 (Q55GV3) RecName: Full=Serine/threonine-protein kinase pakC; ... 450 e-125 Y10158_1(Y10158\|pid:none) D.discoideum mRNA for STE20/pak kinase... 331 4e-89 (Q869N2) RecName: Full=Serine/threonine-protein kinase pakB; ... 331 4e-89 U67716_1(U67716\|pid:none) Dictyostelium discoideum myosin I heav... 331 4e-89 AY870660_1(AY870660\|pid:none) Entamoeba histolytica p21-activate... 312 2e-83 AF131221_1(AF131221\|pid:none) Dictyostelium discoideum p21-activ... 304 5e-81 AE014297_446(AE014297\|pid:none) Drosophila melanogaster chromoso... 250 7e-65 U56080_1(U56080\|pid:none) Drosophila melanogaster p21-activated ... 250 9e-65 BC059992_1(BC059992\|pid:none) Xenopus laevis hypothetical protei... 250 9e-65
protein update	2009. 5.25
PSORT	psg: 0.75 gvh: 0.33 alm: 0.41 top: 0.53 tms: 0.00 mit: 0.21 mip: 0.00 nuc: 0.04 erl: 0.00 erm: 0.20 pox: 0.00 px2: 0.00 vac: 0.00 rnp: 0.00 act: 0.00 caa: 0.00 yqr: 0.00 tyr: 0.00 leu: 0.00 gpi: 0.00 myr: 0.00 dna: 0.00 rib: 0.00 bac: 0.00 m1a: 0.00 m1b: 0.00 m2 : 0.00 mNt: 0.00 m3a: 0.00 m3b: 0.00 m_ : 1.00 56.0 %: cytoplasmic 24.0 %: nuclear 20.0 %: cytoskeletal >> prediction for SFH221 is cyt
5' end seq. ID	SFH221F
5' end seq.	>SFH221F.Seq ATAAAAATGATTTCATTAGAAGATCTATCACCAACATTATGGAAATCACCAGATAAAGAG GGTGAATTAAAGAAACAAGGACATGTAGTAAAGAATTGGAAGAAAAGAAAGTTTATTATT CAAAATGATATGTTATTTTATTTTAAGGATAAAGAGGAGAGACCAGTTGGAGCGGTGCCA TTAAGAATGTCACGTTGCTATGAAAATAAATCATTAGGTAAACCAAATTGTTTCGAATTA GTATCACCAAGAATCAATAAAACATTTTTCATTCAAGCCAATACACCAGATGAAATGGCA AGTTGGATGAAAGCAGTTGAGAAAGGATCAGAATATAGCACAGTATCACAACCATTCAAT CTTAAACATGAGGTACATGTTGATTTCAATTCAGCAACTGGTTTCTCTGGTTTACCAAAA GAATGGGAAGTTATCTTAAAGTCTTCAAATGTTTCAAAACAAGAAGTACTCGATAAACCA AGTGAATGGTTATCAGTTTTAGAATTACAAGCTGGTAGAACAATGGAAAAATCAAATTCT CAAAATTTATCTGCACTTCCTGATGAATCAAATTTAACTCTTAGTGATTTAGTAA----- -----
Length of 5' end seq.	595
3' end seq. ID	SFH221Z
3' end seq.	>SFH221Z.Seq ----------ATGATAATGCCAAACTCTTGGTAACAGAGATTGCAATTATGAAGACATCA CATCATGATAACATTGTAAATTACATTGATAGTTATATAGTGAACGATAGAGAACTTTGG GTTGCAATGGAGTTTATGGGTGGTGGTTGTTTAACAGACATTTTAGAGGCATTTGATAAT ATCAAAATGAGTGAGATTCAAATTGCTTATGTGGTTAAAGAGACCTTGAAGGCATTGCAA TACATTCATAGTCTTCATCGTATTCATAGAGATATAAAGAGTGATAATATTTTATTGGGC TCAGAGGGTAGTGTAAAGATTGCTGATTTTGGTTATGCCGCTCAATTAACTCAAAAACAA CAAAAACGTAATACCGTCGTTGGTACCCCTTATTGGATGGCCCCAGAACTCATTAGAGGT CACGATTATGGTGTCAAGGTTGATATTTGGTCTTTGGGTATCATGATGATGGAAATGGCT GAAGGTGAACCACCTTATATGGATTTCCCACCATTGCGTGCTCTTTTCCTCATCACTACT AGAGGTATTCCACCACTTAAAGAAACAACCAAATGGAGTAAAACTTTCCAAGATTTCTTC TCAAAATGTCTCGATATCAATGTTGCTAATCGTCCTGATGCTACCGATCTTTTAAAACAT CCTTTCATGGATTGGCATGNATTCCTCNAATTTAACCATNATTCAAGCCGCAAGAAA
Length of 3' end seq.	707
Connected seq. ID	SFH221P
Connected seq.	>SFH221P.Seq ATAAAAATGATTTCATTAGAAGATCTATCACCAACATTATGGAAATCACCAGATAAAGAG GGTGAATTAAAGAAACAAGGACATGTAGTAAAGAATTGGAAGAAAAGAAAGTTTATTATT CAAAATGATATGTTATTTTATTTTAAGGATAAAGAGGAGAGACCAGTTGGAGCGGTGCCA TTAAGAATGTCACGTTGCTATGAAAATAAATCATTAGGTAAACCAAATTGTTTCGAATTA GTATCACCAAGAATCAATAAAACATTTTTCATTCAAGCCAATACACCAGATGAAATGGCA AGTTGGATGAAAGCAGTTGAGAAAGGATCAGAATATAGCACAGTATCACAACCATTCAAT CTTAAACATGAGGTACATGTTGATTTCAATTCAGCAACTGGTTTCTCTGGTTTACCAAAA GAATGGGAAGTTATCTTAAAGTCTTCAAATGTTTCAAAACAAGAAGTACTCGATAAACCA AGTGAATGGTTATCAGTTTTAGAATTACAAGCTGGTAGAACAATGGAAAAATCAAATTCT CAAAATTTATCTGCACTTCCTGATGAATCAAATTTAACTCTTAGTGATTTAGTAA----- -----ATGATAATGCCAAACTCTTGGTAACAGAGATTGCAATTATGAAGACATCACATCA TGATAACATTGTAAATTACATTGATAGTTATATAGTGAACGATAGAGAACTTTGGGTTGC AATGGAGTTTATGGGTGGTGGTTGTTTAACAGACATTTTAGAGGCATTTGATAATATCAA AATGAGTGAGATTCAAATTGCTTATGTGGTTAAAGAGACCTTGAAGGCATTGCAATACAT TCATAGTCTTCATCGTATTCATAGAGATATAAAGAGTGATAATATTTTATTGGGCTCAGA GGGTAGTGTAAAGATTGCTGATTTTGGTTATGCCGCTCAATTAACTCAAAAACAACAAAA ACGTAATACCGTCGTTGGTACCCCTTATTGGATGGCCCCAGAACTCATTAGAGGTCACGA TTATGGTGTCAAGGTTGATATTTGGTCTTTGGGTATCATGATGATGGAAATGGCTGAAGG TGAACCACCTTATATGGATTTCCCACCATTGCGTGCTCTTTTCCTCATCACTACTAGAGG TATTCCACCACTTAAAGAAACAACCAAATGGAGTAAAACTTTCCAAGATTTCTTCTCAAA ATGTCTCGATATCAATGTTGCTAATCGTCCTGATGCTACCGATCTTTTAAAACATCCTTT CATGGATTGGCATGNATTCCTCNAATTTAACCATNATTCAAGCCGCAAGAAA
Length of connected seq.	1302
Full length Seq ID	-
Full length Seq.	-
Length of full length seq.	-

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas