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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFH188
Library SF
(Link to library)
Clone ID SFH188
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15677-1
Original site URL
Representative seq. ID SFH188P
(Link to Original site)
Representative DNA sequence 
>SFH188 (SFH188Q) /CSM/SF/SFH1-D/SFH188Q.Seq.d/
AACAACAACAACAACAACAACAACAACAAGTTCAACAACAACAACAACAATTTAATAATT
TTAATTATAGTGGATTAAATGAAGAACAAATTAAATATTATCAACAACAAGCACAATATC
AACAACAGGTTCAACAAGCACAACAAGCAGCAATTCAACAAGCACAACAAGCAGCAGCCT
TAGCTCAAGCATCACAACAACAACTTCAACAACAAGAACAACAAAGACAACAAGCTTCAA
ATTTCTTTTTAGATATGAATGAATATGGTCAACGTATTCGTGTTTGTGTTAGAAAAAGAC
CATTAAATAAAAAAGAAATTGCAAAATCTGAAAAAGATATTATTGAAGTTTTACCAAAAA
AAGATTTAATTGTAAATGAACCAAAAACAAAATTAGATTTAAGTAAATTTATTGAAAAAC
ATAAGTTTACATTTGATGGTGTATTTGATGAAAGTGCAAATAATTATCAAGTTTATTTAC
ATACAGCATATCCATTAGTTGATTCAATTTTTCATAAAGGTAAAGCAACATGTTTTGCAT
ATGGTCAAACTGGTTCAGGTAAAACTCATACTCAAATGGGTCAACAAGGTGATGGTTTAT
ATGCATTGGCAGCACGTGATATATTTCATCGTCTTGGAAACTTATTTTAAAGATCAXXXX
XXXXXXATTTAATATNCCACAAACACCAAAACAACAACAANCAATTCAAACTCAACCACC
AATGTCACCACCAAAAATTGATTTTGTTAATTATCATAGAAATCATGTCGATCAATTTGC
TGATATTTTAAAGAAAGAATTAATTTNTATAAATCAATTTGAATNTTNAAAAGGTTTTAT
TCCATTGGAAAATTATATTAATAATATTGAACAATTTTTAGATTCAAAACAATTACTAAT
TAATCATTTAAGAGGTATAATACAGCAACATCAACAACAACCAATTCAACAACAACAACA
ACAACAACAACCAATTCAACAACAACAACAATCAACTCCTCAACCTTNTCAATTACAAAC
TCCACAACAAAGAGAAAGAGCAAGAAGTCAATTACAACCTCCTAAACCTTCAATTTATAG
TTCAAGAAATTAAAAAAAAAATACCAATACTCATTATACGCATAATATTAC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nnnnnnnnnkfnnnnnnliiliivd*mknklniinnkhninnrfnkhnkqqfnkhnkqqp
*lkhhnnnfnnknnkdnklqisf*i*mnmvnvfvfvlekdh*ikkklqnlkkillkfyqk
ki*l*mnqkqn*i*vnllknislhlmvylmkvqiiikfiyiqhih*LIQFFIKVKQHVLH
MVKLVQVKLILKWVNKVMVYMHWQHVIYFIVLETYFKD---

---FNXPQTPKQQQXIQTQPPMSPPKIDFVNYHRNHVDQFADILKKELIXINQFEXXKGF
IPLENYINNIEQFLDSKQLLINHLRGIIQQHQQQPIQQQQQQQQPIQQQQQSTPQPXQLQ
TPQQRERARSQLQPPKPSIYSSRN*kkntnthythni
Translated Amino Acid sequence (All Frames) 
Frame A:
nnnnnnnnnkfnnnnnnliiliivd*mknklniinnkhninnrfnkhnkqqfnkhnkqqp
*lkhhnnnfnnknnkdnklqisf*i*mnmvnvfvfvlekdh*ikkklqnlkkillkfyqk
ki*l*mnqkqn*i*vnllknislhlmvylmkvqiiikfiyiqhih*LIQFFIKVKQHVLH
MVKLVQVKLILKWVNKVMVYMHWQHVIYFIVLETYFKD---

---i*yxtntktttxnsnsttnvttkn*fc*ls*kscrsic*yfkerinxyksi*xxkrf
ysigkly**y*tifrfktitn*sfkryntatstttnsttttttttnsttttinsstxsit
nsttkrkskksitts*tfnl*fkklkkkyqyslya*yy

Frame B:
tttttttttssttttti**f*l*wik*rtn*ilsttstisttgststtssnststtsssl
sssittttsttrttkttsfkflfrye*iwstysclc*kktik*krncki*kryy*sftkk
rfnck*tknkirfk*iy*kt*vyi*wci**kck*lsslftysisis*fnfs*r*snmfci
wsnwfr*nsysngstr*wficigst*yissswklilki---

---FNXPQTPKQQQXIQTQPPMSPPKIDFVNYHRNHVDQFADILKKELIXINQFEXXKGF
IPLENYINNIEQFLDSKQLLINHLRGIIQQHQQQPIQQQQQQQQPIQQQQQSTPQPXQLQ
TPQQRERARSQLQPPKPSIYSSRN*kkntnthythni

Frame C:
qqqqqqqqqvqqqqqqfnnfnysglneeqikyyqqqaqyqqqvqqaqqaaiqqaqqaaal
aqasqqqlqqqeqqrqqasnffldmneygqrirvcvrkrplnkkeiaksekdiievlpkk
dlivnepktkldlskfiekhkftfdgvfdesannyqvylhtayplvdsifhkgkatcfay
gqtgsgkthtqmgqqgdglyalaardifhrlgnlf*rs---

---lixhkhqnnnxqfklnhqchhqklilliiieimsinllif*rkn*fx*inlnxxkvl
fhwkiiliilnnf*iqnny*lii*ev*ysninnnqfnnnnnnnnqfnnnnnqllnlxnyk
lhnkekeqevnynllnlqfivqeikkkipiliiriil
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH188 (SFH188Q) /CSM/SF/SFH1-D/SFH188Q.Seq.d/                        591   e-168
AHK436 (AHK436Q) /CSM/AH/AHK4-B/AHK436Q.Seq.d/                        383   e-105
AFO805 (AFO805Q) /CSM/AF/AFO8-A/AFO805Q.Seq.d/                        317   2e-85
AHK837 (AHK837Q) /CSM/AH/AHK8-B/AHK837Q.Seq.d/                        283   3e-75
VSI441 (VSI441Q) /CSM/VS/VSI4-B/VSI441Q.Seq.d/                        109   9e-23
AFF307 (AFF307Q) /CSM/AF/AFF3-A/AFF307Q.Seq.d/                         74   5e-12
FC-BK17 (FC-BK17Q) /CSM/FC/FC-BK/FC-BK17Q.Seq.d/                       44   0.005
VHC364 (VHC364Q) /CSM/VH/VHC3-C/VHC364Q.Seq.d/                         42   0.018
SLH656 (SLH656Q) /CSM/SL/SLH6-C/SLH656Q.Seq.d/                         42   0.018
SLB287 (SLB287Q) /CSM/SL/SLB2-D/SLB287Q.Seq.d/                         42   0.018
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY484461|AY484461.1 Dictyostelium discoideum kinesin family member 6 gene, complete cds.   498   0.0    10
U69984|U69984.1 Dictyostelium discoideum kinesin-like protein K6 mRNA, partial cds.   543   0.0    4
AF015712|AF015712.1 Dictyostelium discoideum kinesin-related protein K2 mRNA, partial cds.    68   2e-11  3
AB037280|AB037280.1 Dictyostelium discoideum DdK2 gene for Dd kinesin-related protein K2, complete cds.    68   2e-11  3
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   8e-07  18
AF338869|AF338869.1 Lymantria dispar kinesin-like protein Ldklp1 (klp1) gene, partial cds.    62   2e-05  1
AY162212|AY162212.1 Dictyostelium discoideum kinesin 1 gene, partial cds.    58   2e-05  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    44   7e-05  20
BM608464|BM608464.1 17000687086466 A.Gam.ad.cDNA.blood1 Anopheles gambiae cDNA clone 19600449703472 5', mRNA sequence.    42   8e-05  2
BM582892|BM582892.1 17000687277215 A.Gam.ad.cDNA.blood1 Anopheles gambiae cDNA clone 19600449716301 5', mRNA sequence.    42   8e-05  2
dna update 2003.12.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U69984_1(U69984|pid:none) Dictyostelium discoideum kinesin-like ...   275   2e-72
DQ444242_1(DQ444242|pid:none) Potorous tridactylus MCAK mRNA, co...   144   7e-33
EF092450_1(EF092450|pid:none) Bombyx mori kinesin-like protein a...   142   2e-32
AK150222_1(AK150222|pid:none) Mus musculus bone marrow macrophag...   141   4e-32
AK088047_1(AK088047|pid:none) Mus musculus 2 days neonate thymus...   141   4e-32
(Q62909) RecName: Full=Kinesin-like protein KIF2C; AltName: Full...   141   4e-32
AY891611_1(AY891611|pid:none) Synthetic construct Homo sapiens c...   141   5e-32
AB264115_1(AB264115|pid:none) Homo sapiens MCAK/KIF2c V1 mRNA fo...   141   5e-32
AY026505_1(AY026505|pid:none) Homo sapiens testis specific mitot...   141   5e-32
(Q99661) RecName: Full=Kinesin-like protein KIF2C; AltName: Full...   141   5e-32
protein update 2009. 5.25
PSORT 

 psg: 0.68  gvh: 0.25  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal

>> prediction for SFH188 is cyt
5' end seq. ID SFH188F
5' end seq. 
>SFH188F.Seq
AACAACAACAACAACAACAACAACAACAAGTTCAACAACAACAACAACAATTTAATAATT
TTAATTATAGTGGATTAAATGAAGAACAAATTAAATATTATCAACAACAAGCACAATATC
AACAACAGGTTCAACAAGCACAACAAGCAGCAATTCAACAAGCACAACAAGCAGCAGCCT
TAGCTCAAGCATCACAACAACAACTTCAACAACAAGAACAACAAAGACAACAAGCTTCAA
ATTTCTTTTTAGATATGAATGAATATGGTCAACGTATTCGTGTTTGTGTTAGAAAAAGAC
CATTAAATAAAAAAGAAATTGCAAAATCTGAAAAAGATATTATTGAAGTTTTACCAAAAA
AAGATTTAATTGTAAATGAACCAAAAACAAAATTAGATTTAAGTAAATTTATTGAAAAAC
ATAAGTTTACATTTGATGGTGTATTTGATGAAAGTGCAAATAATTATCAAGTTTATTTAC
ATACAGCATATCCATTAGTTGATTCAATTTTTCATAAAGGTAAAGCAACATGTTTTGCAT
ATGGTCAAACTGGTTCAGGTAAAACTCATACTCAAATGGGTCAACAAGGTGATGGTTTAT
ATGCATTGGCAGCACGTGATATATTTCATCGTCTTGGAAACTTATTTTAAAGATCA----
------
Length of 5' end seq. 656
3' end seq. ID SFH188Z
3' end seq. 
>SFH188Z.Seq
----------ATTTAATATNCCACAAACACCAAAACAACAACAANCAATTCAAACTCAAC
CACCAATGTCACCACCAAAAATTGATTTTGTTAATTATCATAGAAATCATGTCGATCAAT
TTGCTGATATTTTAAAGAAAGAATTAATTTNTATAAATCAATTTGAATNTTNAAAAGGTT
TTATTCCATTGGAAAATTATATTAATAATATTGAACAATTTTTAGATTCAAAACAATTAC
TAATTAATCATTTAAGAGGTATAATACAGCAACATCAACAACAACCAATTCAACAACAAC
AACAACAACAACAACCAATTCAACAACAACAACAATCAACTCCTCAACCTTNTCAATTAC
AAACTCCACAACAAAGAGAAAGAGCAAGAAGTCAATTACAACCTCCTAAACCTTCAATTT
ATAGTTCAAGAAATTAAAAAAAAAATACCAATACTCATTATACGCATAATATTAC
Length of 3' end seq. 465
Connected seq. ID SFH188P
Connected seq. 
>SFH188P.Seq
AACAACAACAACAACAACAACAACAACAAGTTCAACAACAACAACAACAATTTAATAATT
TTAATTATAGTGGATTAAATGAAGAACAAATTAAATATTATCAACAACAAGCACAATATC
AACAACAGGTTCAACAAGCACAACAAGCAGCAATTCAACAAGCACAACAAGCAGCAGCCT
TAGCTCAAGCATCACAACAACAACTTCAACAACAAGAACAACAAAGACAACAAGCTTCAA
ATTTCTTTTTAGATATGAATGAATATGGTCAACGTATTCGTGTTTGTGTTAGAAAAAGAC
CATTAAATAAAAAAGAAATTGCAAAATCTGAAAAAGATATTATTGAAGTTTTACCAAAAA
AAGATTTAATTGTAAATGAACCAAAAACAAAATTAGATTTAAGTAAATTTATTGAAAAAC
ATAAGTTTACATTTGATGGTGTATTTGATGAAAGTGCAAATAATTATCAAGTTTATTTAC
ATACAGCATATCCATTAGTTGATTCAATTTTTCATAAAGGTAAAGCAACATGTTTTGCAT
ATGGTCAAACTGGTTCAGGTAAAACTCATACTCAAATGGGTCAACAAGGTGATGGTTTAT
ATGCATTGGCAGCACGTGATATATTTCATCGTCTTGGAAACTTATTTTAAAGATCA----
------ATTTAATATNCCACAAACACCAAAACAACAACAANCAATTCAAACTCAACCACC
AATGTCACCACCAAAAATTGATTTTGTTAATTATCATAGAAATCATGTCGATCAATTTGC
TGATATTTTAAAGAAAGAATTAATTTNTATAAATCAATTTGAATNTTNAAAAGGTTTTAT
TCCATTGGAAAATTATATTAATAATATTGAACAATTTTTAGATTCAAAACAATTACTAAT
TAATCATTTAAGAGGTATAATACAGCAACATCAACAACAACCAATTCAACAACAACAACA
ACAACAACAACCAATTCAACAACAACAACAATCAACTCCTCAACCTTNTCAATTACAAAC
TCCACAACAAAGAGAAAGAGCAAGAAGTCAATTACAACCTCCTAAACCTTCAATTTATAG
TTCAAGAAATTAAAAAAAAAATACCAATACTCATTATACGCATAATATTAC
Length of connected seq. 1121
Full length Seq ID -
Full length Seq. -
Length of full length seq. -