>SFH187 (SFH187Q) /CSM/SF/SFH1-D/SFH187Q.Seq.d/
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAG
GCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTG
CCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATG
TCGCAGAXXXXXXXXXXCTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCA
AGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATT
TGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGA
TAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGC
TTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGC
CGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGT
TTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGT
TGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGA
AATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATAT
GAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGG
TGCCCGTCAAATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGT
AATCTCTAT

FSYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVA
QEDPIISGCYNTMGQTSENVA---

---ENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDE
GIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVA
FAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKV
NPNGSGIALGHPLGATGARQMATISRELHRRKGKYGVIS

Frame A:
ifhiyi*yytp*likchsiqelklflvt*aldqf*nqiqlldqlnqemil*llhhfvqqf
vklkeehskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvqpklelphy*
qvyqrkyhviqstdnvhqvykqlpivqqqskqvsmtlv*qav*nq*v*iqwhgmvvstrl
pkriqslvvvttlwvkplkmsq---

---lkmsqsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp*rprmakpsssikt
kvsvrkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*
llllsvsnhplwvlvqplqfqpp*nklvsplkistflksmkllllkhftvvrfwvwi*tk
liqmalvsl*vih*vllvpvkwlpfqenyivvkvnmv*sl

Frame B:
ffifiynitphn**nviqfkn*nyfsslkhwtnfriksnfwis*irr*ycncctisysnl
*s*krsiqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcnrs*schiis
rftkgstmlysqqtmfirftsncqlcsssqsrfl*hwfsrrcrinefksngmgwwfqrgc
prgsnh*wllqhygsnl*kcrr---

---*kcrrafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rr
ryp*enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrs
fcccrcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfssilql*gfgfgyeqs
*skwlwyrfrssircywcpsngyhfkritss*r*iwcnly

Frame C:
FSYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVA
QEDPIISGCYNTMGQTSENVA---

---ENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDE
GIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVA
FAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKV
NPNGSGIALGHPLGATGARQMATISRELHRRKGKYGVIS

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH187 (SFH187Q) /CSM/SF/SFH1-D/SFH187Q.Seq.d/                       2456   0.0
VFE239 (VFE239Q) /CSM/VF/VFE2-B/VFE239Q.Seq.d/                       1292   0.0
SLG762 (SLG762Q) /CSM/SL/SLG7-C/SLG762Q.Seq.d/                       1292   0.0
SFI740 (SFI740Q) /CSM/SF/SFI7-B/SFI740Q.Seq.d/                       1292   0.0
SFH501 (SFH501Q) /CSM/SF/SFH5-A/SFH501Q.Seq.d/                       1292   0.0
SFG758 (SFG758Q) /CSM/SF/SFG7-C/SFG758Q.Seq.d/                       1292   0.0
SFG482 (SFG482Q) /CSM/SF/SFG4-D/SFG482Q.Seq.d/                       1292   0.0
SFG475 (SFG475Q) /CSM/SF/SFG4-D/SFG475Q.Seq.d/                       1292   0.0
SFA524 (SFA524Q) /CSM/SF/SFA5-A/SFA524Q.Seq.d/                       1292   0.0
CHB394 (CHB394Q) /CSM/CH/CHB3-D/CHB394Q.Seq.d/                       1292   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strainAX4, complete sequence.  1292   0.0    9
CA917334|CA917334.1 EST641481 GPOD Medicago truncatula cDNA clone GPOD-35H18, mRNA sequence.    40   4e-18  7
CA858588|CA858588.1 EST635843 GLSD Medicago truncatula cDNA clone pGLSD-38M16, mRNA sequence.    40   3e-14  6
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60   3e-13  5
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44   1e-12  5
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60   2e-11  4
CF213585|CF213585.1 CGF1000794_F07 Vitis vinifera cv. cabernet sauvignon Stem - CAST Vitis vinifera cDNA clone CAST0006_IF_F07 5', mRNA sequence.    60   7e-10  3
CB893643|CB893643.1 EST646435 HOGA Medicago truncatula cDNA clone HOGA-28F15, mRNA sequence.    40   6e-09  5
CD007738|CD007738.1 VVB078G11_334604 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB078G11 5, mRNA sequence.    60   3e-08  2
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68   3e-08  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   396   0.0
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   236   e-111
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   236   e-110
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   234   e-110
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   233   e-109
(Q56WD9) RecName: Full=3-ketoacyl-CoA thiolase 2, peroxisomal;  ...   237   e-109
T07989(T07989) acetyl-CoA C-acyltransferase (EC 2.3.1.16) precur...   236   e-109
BT070433_1(BT070433|pid:none) Picea sitchensis clone WS02729_F15...   234   e-108
AX025481_1(AX025481|pid:none) Sequence 7 from Patent WO0032789.       232   e-108
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   240   e-108

 psg: 0.91  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.33  mip: 0.05
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SFH187 is nuc

>SFH187F.Seq
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAG
GCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTG
CCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATG
TCGCAGA----------

>SFH187Z.Seq
----------CTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAA
TTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGAT
GAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGAC
GAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAA
GAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGT
TTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTA
GCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATT
CCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAAT
GAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAA
GTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGT
CAAATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCT
AT

>SFH187P.Seq
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAG
GCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTG
CCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATG
TCGCAGA----------CTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCA
AGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATT
TGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGA
TAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGC
TTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGC
CGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGT
TTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGT
TGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGA
AATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATAT
GAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGG
TGCCCGTCAAATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGT
AATCTCTAT