>SFH171 (SFH171Q) /CSM/SF/SFH1-C/SFH171Q.Seq.d/
XXXXXXXXXXAAGAAATCANCAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTG
GTCATTTCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGT
AACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCC
AAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCC
GGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGG
AATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGT
GAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTA
ATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCT
ATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACC
GACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCC
GAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGAC
GAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAA
GAAGAAGTCAAAAAATCAACCAAAAAAGTNATCAGACGAACTTTA

---EIXKVETDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADDWELLPSKQIKDPKQSK
PVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGE
WKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTD
SVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEE
EVKKSTKKVIRRTL

Frame A:
---rnxqs*nrsinssihfghftr*hlqstrr*qrdpsr*pr**lgitsiktnqrsktiq
tsrlg*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*
meskenpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhr
lcrrs*rfl*kdlccqprsrkenv*rprsrqs*rekes*rkiscrksc*krsrrs**rrr
rsqkinqksxqtnf


Frame B:
---EIXKVETDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADDWELLPSKQIKDPKQSK
PVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGE
WKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTD
SVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEE
EVKKSTKKVIRRTL


Frame C:
---ksxklkpin*lintlwsfhqitptkyssitkrskqvtslmignyfhqnkskiqnnpn
qsiglt*rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvn
gkqrksqiqntkvngyth*litqntlktmnstslmisvlsvsnygklnlvpslti*sspt
lskklkislkrpllptkkpkrkclttskppklkrerkltkn*lpkkllkkkpkklmkkkk
ksknqpkkxsdel

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFH171 (SFH171Q) /CSM/SF/SFH1-C/SFH171Q.Seq.d/                       1243   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1243   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1243   0.0
AFE410 (AFE410Q) /CSM/AF/AFE4-A/AFE410Q.Seq.d/                       1243   0.0
AFE318 (AFE318Q) /CSM/AF/AFE3-A/AFE318Q.Seq.d/                       1243   0.0
AFE168 (AFE168Q) /CSM/AF/AFE1-C/AFE168Q.Seq.d/                       1243   0.0
AFD463 (AFD463Q) /CSM/AF/AFD4-C/AFD463Q.Seq.d/                       1243   0.0
AFJ570 (AFJ570Q) /CSM/AF/AFJ5-C/AFJ570Q.Seq.d/                       1237   0.0
AFA657 (AFA657Q) /CSM/AF/AFA6-C/AFA657Q.Seq.d/                       1237   0.0
AFK245 (AFK245Q) /CSM/AF/AFK2-B/AFK245Q.Seq.d/                       1235   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1235   0.0    2
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-15  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   5e-11  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   3e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74   3e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74   3e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74   3e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74   3e-09  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    74   3e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74   3e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   390   e-107
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   251   2e-65
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   241   2e-62
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   237   3e-61
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   234   2e-60
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   234   2e-60
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   234   3e-60
AC149080_5(AC149080|pid:none) Medicago truncatula clone mth2-75j...   233   5e-60
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   232   8e-60
AK104328_1(AK104328|pid:none) Oryza sativa Japonica Group cDNA c...   232   8e-60

 psg: 0.75  gvh: 0.25  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.10  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 20.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SFH171 is nuc

>SFH171Z.Seq
----------AAGAAATCANCAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTG
GTCATTTCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGT
AACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCC
AAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCC
GGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGG
AATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGT
GAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTA
ATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCT
ATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACC
GACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCC
GAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGAC
GAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAA
GAAGAAGTCAAAAAATCAACCAAAAAAGTNATCAGACGAACTTTA