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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFH150
Library SF
(Link to library)
Clone ID SFH150
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11996-1
Original site URL
Representative seq. ID SFH150P
(Link to Original site)
Representative DNA sequence 
>SFH150 (SFH150Q) /CSM/SF/SFH1-C/SFH150Q.Seq.d/
TGGCCTACTGGAAAATTACAGACATTTAGCAATAAATATAAAAGTACCAGTACATTTACT
TACAACCCTATTTAATAGTAGGGATAGTTAATAATATAAATATAAATAACAACAATAAGA
ACAATAAAAAAAAAAXXXXXXXXXXTTCATTTACAGAAAAAGGTGCACGTAATGTTGTAA
GACAAGTATGTGCTGGTGTTGAATATCTCCATTCACAAGGTATTGCTCATAGAGATTTGA
AACCAGAGAATTTATTATGTAATGGTGAAGGTGAAGATATGACAATCAAAATTGCAGATT
TTGGTCTTAGTAAAATATTTGGAACTGGTGAAGCATTGNAAACAAGTTGTGGTACACCAG
ATTATGTTGCACCAGAAGTTTTAANTGGTGGTTCATATGATAATGCAGTCGATATGTGGT
CAATTGGTGTCATTACCTATATTTTATTATGTGGTTTCCCACCATTCTATGCTTCATCTC
AAAACTTATTATTTGAAAAAATTTTANCCGCTGATTATGATTTCCCAGAACCAGAATGGA
CTCATGTCTCTGAACATGCCAAAGCTTTCATTAGAAACTTAATTGTCAAAGATCCAGATC
AACGTCATACTGCCAAACAATGTCTTGATGACCTTTGGCTCTCTGGTAGTGATCAATCAA
TTGGTTCNGCTGCTGATCTCCACTCTCATTNC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
aywkitdi*q*i*KYQYIYLQPYLIVGIVNNININNNNKNNKKK---

---SFTEKGARNVVRQVCAGVEYLHSQGIAHRDLKPENLLCNGEGEDMTIKIADFGLSKI
FGTGEALXTSCGTPDYVAPEVLXGGSYDNAVDMWSIGVITYILLCGFPPFYASSQNLLFE
KILXADYDFPEPEWTHVSEHAKAFIRNLIVKDPDQRHTAKQCLDDLWLSGSDQSIGSAAD
LHSHX
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgklqtfsnkykststftynpi***g*lii*i*ittirtikkk---

---fiyrkrct*ccktsmcwc*ispftrycs*rfetrefim*w*r*rydnqncrfws**n
iwnw*sixnklwytrlcctrsfxwwfi**csryvvnwchylyfimwfptilcfisklii*
knfxr*l*fprtrmdscl*tcqsfh*klncqrsrstsycqtms**plalw**sinwfxc*
splsx

Frame B:
gllenyrhlainikvpvhllttlfnsrds**ykyk*qq*eq*kk---

---SFTEKGARNVVRQVCAGVEYLHSQGIAHRDLKPENLLCNGEGEDMTIKIADFGLSKI
FGTGEALXTSCGTPDYVAPEVLXGGSYDNAVDMWSIGVITYILLCGFPPFYASSQNLLFE
KILXADYDFPEPEWTHVSEHAKAFIRNLIVKDPDQRHTAKQCLDDLWLSGSDQSIGSAAD
LHSHX

Frame C:
aywkitdi*q*i*KYQYIYLQPYLIVGIVNNININNNNKNNKKK---

---hlqkkvhvml*dkyvlvlnisihkvlliei*nqriyyvmvkvki*qsklqilvlvky
lelvkhxkqvvvhqimlhqkf*xvvhmimqsicgqlvslpifyyvvshhsmlhlktyylk
kfxplimisqnqnglmslnmpklslet*lskiqinvilpnnvlmtfgslvvinqlvxlli
stli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSI101 (SSI101Q) /CSM/SS/SSI1-A/SSI101Q.Seq.d/                       1057   0.0
SFK820 (SFK820Q) /CSM/SF/SFK8-A/SFK820Q.Seq.d/                       1057   0.0
SFH150 (SFH150Q) /CSM/SF/SFH1-C/SFH150Q.Seq.d/                       1057   0.0
AFL780 (AFL780Q) /CSM/AF/AFL7-D/AFL780Q.Seq.d/                       1041   0.0
SSC643 (SSC643Q) /CSM/SS/SSC6-B/SSC643Q.Seq.d/                       1033   0.0
CFB218 (CFB218Q) /CSM/CF/CFB2-A/CFB218Q.Seq.d/                       1023   0.0
VFJ379 (VFJ379Q) /CSM/VF/VFJ3-D/VFJ379Q.Seq.d/                       1019   0.0
VFG501 (VFG501Q) /CSM/VF/VFG5-A/VFG501Q.Seq.d/                       1013   0.0
VFG381 (VFG381Q) /CSM/VF/VFG3-D/VFG381Q.Seq.d/                       1011   0.0
VSF265 (VSF265Q) /CSM/VS/VSF2-C/VSF265Q.Seq.d/                        999   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strainAX4, complete sequence.  1057   0.0    13
M64176|M64176.1 D.discoideum myosin light chain kinase (MLCK) mRNA, complete cds.    84   6e-27  3
AB118103|AB118103.1 Entamoeba histolytica gene for hypothetical protein, complete cds, clone:Eh-2109.    52   1e-16  4
AZ667950|AZ667950.1 ENTFN88TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-14  3
AZ545332|AZ545332.1 ENTGA35TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   1e-10  3
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    50   1e-08  11
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    56   3e-08  15
AC115685|AC115685.1 Dictyostelium discoideum chromosome 2 map 4718821-4752388 strain AX4, complete sequence.    56   7e-07  2
BH137166|BH137166.1 ENTNR83TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-06  2
AZ672839|AZ672839.1 ENTIX25TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   3e-06  2
dna update 2003.12.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W6) RecName: Full=Probable calcium/calmodulin-dependent ser...   370   e-101
AC116982_54(AC116982|pid:none) Dictyostelium discoideum chromoso...   215   9e-55
AB076903_1(AB076903|pid:none) Ciona intestinalis Ci-CaM-K mRNA f...   197   2e-49
BC074183_1(BC074183|pid:none) Xenopus laevis MGC82022 protein, m...   197   3e-49
AK052401_1(AK052401|pid:none) Mus musculus 13 days embryo lung c...   195   1e-48
(Q8IU85) RecName: Full=Calcium/calmodulin-dependent protein kina...   195   1e-48
AY335686_1(AY335686|pid:none) Synthetic construct Homo sapiens C...   195   1e-48
(Q8BW96) RecName: Full=Calcium/calmodulin-dependent protein kina...   195   1e-48
BC110966_1(BC110966|pid:none) Xenopus laevis calcium/calmodulin-...   193   5e-48
BC041721_1(BC041721|pid:none) Xenopus laevis calcium/calmodulin-...   191   2e-47
protein update 2009. 5.25
PSORT 

 psg: 0.90  gvh: 0.33  alm: 0.34  top: 0.73  tms: 0.00  mit: 0.25  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for SFH150 is cyt
5' end seq. ID SFH150F
5' end seq. 
>SFH150F.Seq
TGGCCTACTGGAAAATTACAGACATTTAGCAATAAATATAAAAGTACCAGTACATTTACT
TACAACCCTATTTAATAGTAGGGATAGTTAATAATATAAATATAAATAACAACAATAAGA
ACAATAAAAAAAAAA----------
Length of 5' end seq. 135
3' end seq. ID SFH150Z
3' end seq. 
>SFH150Z.Seq
----------TTCATTTACAGAAAAAGGTGCACGTAATGTTGTAAGACAAGTATGTGCTG
GTGTTGAATATCTCCATTCACAAGGTATTGCTCATAGAGATTTGAAACCAGAGAATTTAT
TATGTAATGGTGAAGGTGAAGATATGACAATCAAAATTGCAGATTTTGGTCTTAGTAAAA
TATTTGGAACTGGTGAAGCATTGNAAACAAGTTGTGGTACACCAGATTATGTTGCACCAG
AAGTTTTAANTGGTGGTTCATATGATAATGCAGTCGATATGTGGTCAATTGGTGTCATTA
CCTATATTTTATTATGTGGTTTCCCACCATTCTATGCTTCATCTCAAAACTTATTATTTG
AAAAAATTTTANCCGCTGATTATGATTTCCCAGAACCAGAATGGACTCATGTCTCTGAAC
ATGCCAAAGCTTTCATTAGAAACTTAATTGTCAAAGATCCAGATCAACGTCATACTGCCA
AACAATGTCTTGATGACCTTTGGCTCTCTGGTAGTGATCAATCAATTGGTTCNGCTGCTG
ATCTCCACTCTCATTNC
Length of 3' end seq. 547
Connected seq. ID SFH150P
Connected seq. 
>SFH150P.Seq
TGGCCTACTGGAAAATTACAGACATTTAGCAATAAATATAAAAGTACCAGTACATTTACT
TACAACCCTATTTAATAGTAGGGATAGTTAATAATATAAATATAAATAACAACAATAAGA
ACAATAAAAAAAAAA----------TTCATTTACAGAAAAAGGTGCACGTAATGTTGTAA
GACAAGTATGTGCTGGTGTTGAATATCTCCATTCACAAGGTATTGCTCATAGAGATTTGA
AACCAGAGAATTTATTATGTAATGGTGAAGGTGAAGATATGACAATCAAAATTGCAGATT
TTGGTCTTAGTAAAATATTTGGAACTGGTGAAGCATTGNAAACAAGTTGTGGTACACCAG
ATTATGTTGCACCAGAAGTTTTAANTGGTGGTTCATATGATAATGCAGTCGATATGTGGT
CAATTGGTGTCATTACCTATATTTTATTATGTGGTTTCCCACCATTCTATGCTTCATCTC
AAAACTTATTATTTGAAAAAATTTTANCCGCTGATTATGATTTCCCAGAACCAGAATGGA
CTCATGTCTCTGAACATGCCAAAGCTTTCATTAGAAACTTAATTGTCAAAGATCCAGATC
AACGTCATACTGCCAAACAATGTCTTGATGACCTTTGGCTCTCTGGTAGTGATCAATCAA
TTGGTTCNGCTGCTGATCTCCACTCTCATTNC
Length of connected seq. 682
Full length Seq ID -
Full length Seq. -
Length of full length seq. -