NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFG632
Library SF
(Link to library)
Clone ID SFG632
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13185-1|Contig-U16260-1
Original site URL
Representative seq. ID SFG632P
(Link to Original site)
Representative DNA sequence 
>SFG632 (SFG632Q) /CSM/SF/SFG6-B/SFG632Q.Seq.d/
TGTCCATGTGGTAACATTGTTGGTTTAGTCGGTGTCGATCAATTCCTTGTCAAATCTGGT
ACCATCACCACCTCTGAAGTTGCTCACAACATTCGTGTCATGAAATTCTCCGTATCACCA
GTCGTCCGTGTTGCCGTCGAACCAAAGAATCCATCTGATTTACCAAAACTCGTCGAAGGT
CTCAAACGTTTAGCCAAATCCGATCCATGTGTACTCTGTTACTCTGAAGAATCTGGTGAA
CACATCGTTGCCGGTGCTGGTGAACTCCATCTCGAAATTTGTCTTAAAGATCTTGCTGAA
GATCACGCTGGTATTGAAATTAAAACCACCGATCCAGTCGTATCATTCAGAGAATCCGTC
TCAGAAGAATCATCAATCATGTGTCTTTCTAAATCACCAAACAAACACAATCGTCTCTTC
ATGAAAGCTTCACCAATCTCTATGGAATTACAAGATCTCATTGAAGCTGGTTCTGATATC
TCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATXXXXXXXXXXGANTT
ANGNTNCCNCNCTGCCGGNCAAGCTTTNCCCCAATGNGNNTTCGANCNTTGGGNNTCAAN
TGGNGNTGNCAACAAAAANAAAAAAGCCCCTGAANTCNCNTTANCCCCCAAAAAAAAAAA
ANGTTTAGCCCC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
CPCGNIVGLVGVDQFLVKSGTITTSEVAHNIRVMKFSVSPVVRVAVEPKNPSDLPKLVEG
LKRLAKSDPCVLCYSEESGEHIVAGAGELHLEICLKDLAEDHAGIEIKTTDPVVSFRESV
SEESSIMCLSKSPNKHNRLFMKASPISMELQDLIEAGSDISSKDDPKARANYLAD---

---XLXXXXAGQAXPQXXFXXWXSXGXXNKXKKAPEXXLXPKKKKXLA
Translated Amino Acid sequence (All Frames) 
Frame A:
CPCGNIVGLVGVDQFLVKSGTITTSEVAHNIRVMKFSVSPVVRVAVEPKNPSDLPKLVEG
LKRLAKSDPCVLCYSEESGEHIVAGAGELHLEICLKDLAEDHAGIEIKTTDPVVSFRESV
SEESSIMCLSKSPNKHNRLFMKASPISMELQDLIEAGSDISSKDDPKARANYLAD---

---XLXXXXAGQAXPQXXFXXWXSXGXXNKXKKAPEXXLXPKKKKXLA

Frame B:
vhvvtllv*svsinslsnlvpspplkllttfvs*nspyhqssvlpsnqrihliyqnsskv
snv*pnpihvysvtlknlvntslpvlvnsiskfvlkillkitlvlklkppiqsyhsenps
qknhqscvflnhqtntivss*klhqslwnykislklvlishpkmiqklvltisl---

---xxxxxlpxklxpnxxsxxgxqxxxxtkxkkplxsxxppkkkxv*p

Frame C:
smw*hcwfsrcrsipcqiwyhhhl*scsqhscheilritsrpccrrtkesi*ftktrrrs
qtfsqirsmctlll*riw*thrcrcw*tpsrnls*rsc*rsrwy*n*nhrssriiqrirl
rriinhvsf*itkqtqsslhesftnlygitrsh*swf*yliqr*skssc*lsr*---

---xxxpxcrxsfxpmxxrxlgxxwxxqqkxksp*xxxxpqkkkxfsp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFG632 (SFG632Q) /CSM/SF/SFG6-B/SFG632Q.Seq.d/                       1043   0.0
CFC418 (CFC418Q) /CSM/CF/CFC4-A/CFC418Q.Seq.d/                       1031   0.0
VFK572 (VFK572Q) /CSM/VF/VFK5-C/VFK572Q.Seq.d/                       1011   0.0
SFE387 (SFE387Q) /CSM/SF/SFE3-D/SFE387Q.Seq.d/                        940   0.0
VFH373 (VFH373Q) /CSM/VF/VFH3-D/VFH373Q.Seq.d/                        932   0.0
VHH341 (VHH341Q) /CSM/VH/VHH3-B/VHH341Q.Seq.d/                        926   0.0
VFH258 (VFH258Q) /CSM/VF/VFH2-C/VFH258Q.Seq.d/                        858   0.0
VHO334 (VHO334Q) /CSM/VH/VHO3-B/VHO334Q.Seq.d/                        741   0.0
VHO801 (VHO801Q) /CSM/VH/VHO8-A/VHO801Q.Seq.d/                        609   e-173
CHE141 (CHE141Q) /CSM/CH/CHE1-B/CHE141Q.Seq.d/                        505   e-142
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   714   0.0    2
CA755388|0    76   3e-43  4
BI502302|BI502302.1 kt87h04.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    68   2e-28  4
CD420587|CD420587.1 ku88h03.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    68   3e-28  4
CD524650|CD524650.1 kw06d07.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    68   3e-28  4
BJ111491|BJ111491.1 Caenorhabditis elegans cDNA clone:yk1143h07 : 5' end, single read.   131   1e-27  2
BJ115053|BJ115053.1 Caenorhabditis elegans cDNA clone:yk1187a06 : 5' end, single read.   131   1e-27  2
BJ104152|BJ104152.1 Caenorhabditis elegans cDNA clone:yk1058e04 : 5' end, single read.   131   1e-27  2
AU205829|AU205829.1 Caenorhabditis elegans cDNA clone:yk855f05 : 5' end, single read.   131   1e-27  2
BJ140438|BJ140438.1 Caenorhabditis elegans cDNA clone:yk1158c07 : 3' end, single read.   131   2e-27  2
dna update 2003.12.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   244   1e-63
AY310293_1(AY310293|pid:none) Scolopendra viridis voucher Svi el...   244   1e-63
AY310283_1(AY310283|pid:none) Rhysida nuda voucher Rnu elongatio...   244   1e-63
AF240828_1(AF240828|pid:none) Scolopendra polymorpha elongation ...   244   1e-63
AY310263_1(AY310263|pid:none) Hiltonius sp. 'Hil' elongation fac...   242   8e-63
AY310274_1(AY310274|pid:none) Orthoporus ornata voucher Oor3 elo...   241   1e-62
AY310269_1(AY310269|pid:none) Narceus americanus voucher Nam elo...   241   2e-62
AY310296_1(AY310296|pid:none) Theatops posticus voucher Tpo elon...   240   3e-62
AY310282_1(AY310282|pid:none) Ribautia sp. 'Rib' elongation fact...   240   3e-62
AY310276_1(AY310276|pid:none) Pokabius bilabiatus voucher Pbi el...   239   4e-62
protein update 2009. 5.25
PSORT 

 psg: 0.87  gvh: 0.39  alm: 0.39  top: 0.40  tms: 0.00  mit: 0.15  mip: 0.08
 nuc: 0.13  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SFG632 is cyt
5' end seq. ID SFG632F
5' end seq. 
>SFG632F.Seq
TGTCCATGTGGTAACATTGTTGGTTTAGTCGGTGTCGATCAATTCCTTGTCAAATCTGGT
ACCATCACCACCTCTGAAGTTGCTCACAACATTCGTGTCATGAAATTCTCCGTATCACCA
GTCGTCCGTGTTGCCGTCGAACCAAAGAATCCATCTGATTTACCAAAACTCGTCGAAGGT
CTCAAACGTTTAGCCAAATCCGATCCATGTGTACTCTGTTACTCTGAAGAATCTGGTGAA
CACATCGTTGCCGGTGCTGGTGAACTCCATCTCGAAATTTGTCTTAAAGATCTTGCTGAA
GATCACGCTGGTATTGAAATTAAAACCACCGATCCAGTCGTATCATTCAGAGAATCCGTC
TCAGAAGAATCATCAATCATGTGTCTTTCTAAATCACCAAACAAACACAATCGTCTCTTC
ATGAAAGCTTCACCAATCTCTATGGAATTACAAGATCTCATTGAAGCTGGTTCTGATATC
TCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGAT----------
Length of 5' end seq. 525
3' end seq. ID SFG632Z
3' end seq. 
>SFG632Z.Seq
----------GANTTANGNTNCCNCNCTGCCGGNCAAGCTTTNCCCCAATGNGNNTTCGA
NCNTTGGGNNTCAANTGGNGNTGNCAACAAAAANAAAAAAGCCCCTGAANTCNCNTTANC
CCCCAAAAAAAAAAAANGTTTAGCCCC
Length of 3' end seq. 137
Connected seq. ID SFG632P
Connected seq. 
>SFG632P.Seq
TGTCCATGTGGTAACATTGTTGGTTTAGTCGGTGTCGATCAATTCCTTGTCAAATCTGGT
ACCATCACCACCTCTGAAGTTGCTCACAACATTCGTGTCATGAAATTCTCCGTATCACCA
GTCGTCCGTGTTGCCGTCGAACCAAAGAATCCATCTGATTTACCAAAACTCGTCGAAGGT
CTCAAACGTTTAGCCAAATCCGATCCATGTGTACTCTGTTACTCTGAAGAATCTGGTGAA
CACATCGTTGCCGGTGCTGGTGAACTCCATCTCGAAATTTGTCTTAAAGATCTTGCTGAA
GATCACGCTGGTATTGAAATTAAAACCACCGATCCAGTCGTATCATTCAGAGAATCCGTC
TCAGAAGAATCATCAATCATGTGTCTTTCTAAATCACCAAACAAACACAATCGTCTCTTC
ATGAAAGCTTCACCAATCTCTATGGAATTACAAGATCTCATTGAAGCTGGTTCTGATATC
TCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGAT----------GANTT
ANGNTNCCNCNCTGCCGGNCAAGCTTTNCCCCAATGNGNNTTCGANCNTTGGGNNTCAAN
TGGNGNTGNCAACAAAAANAAAAAAGCCCCTGAANTCNCNTTANCCCCCAAAAAAAAAAA
ANGTTTAGCCCC
Length of connected seq. 662
Full length Seq ID -
Full length Seq. -
Length of full length seq. -