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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFG249
Library SF
(Link to library)
Clone ID SFG249
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16361-1
Original site URL
Representative seq. ID SFG249P
(Link to Original site)
Representative DNA sequence 
>SFG249 (SFG249Q) /CSM/SF/SFG2-C/SFG249Q.Seq.d/
GTGAAAAGATTTCCAACTTTACCATTGACTCCCTCCAATCATTATGGCATGGTTCAGCAT
TCTTCCGTCCATTGATTCGTTCAATTCTCTTAAAGAAAGATTATGTTAGAAACTTTGTTT
CCGAATTGAATAATCAACATCGTCTTAGATTCCTCAATACTGAAGCATTAGCAATGGCAC
AATATCAAGTGTTGATGATGGGTAGACAAGAGTTACCATGGGATGAAATCTCTTCCGGTA
ATGTACGTATAGATTTCGATACTTGTGATGGTCCAATAGTTTCACCAAAATGTACCTACC
TTCGTAGACGTAAACCACCCATTCCACATAATAAAGCAGGTAACATCAATAATGCCCTCT
TTAATGAATCAACTAAAGCCGATTACGAGTTCCTTGGTTTACTTGATGCAGATCAACAAC
CTCATCCAGATTTCCTCAAAAGAGTTTTACCATACTTTTACAGTGATGAAGGTCAAGATT
TGGCTTTTGTTCAAACACCACAATTCTTTAGTAACATTTATCXXXXXXXXXXGCTTCTCC
ATTAATTATGAGTATTTGGACTGTACCAATCGTTGTAAAAGATCCAATCATTTTCATTCT
CGTCGGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTCAATATATGATTCTTAGAGC
CAAGAGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGGGTAGAAGCTACTGATCT
TTGGAGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTTATATTTCATCATGGAGAGA
AGGTTCCGCTTCAATCGTTAAACTTCTTAAAGCTAGAAAGATTAGTCGTCATAAGTTAGC
CATGTGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTTTGTGAAGTTTTCCGTCA
AACTAAATTGGTCAATGAAAATGATAATGCTCAAGAATCAAGTGGTAAACATAAGGCTGA
ACAATCTTTCCGTACTTCAAACAAAGAATCTGATACCATTAAAAATTCAAGACTTTTCCT
TCCAAATATTATCCTCTTTGTTGTTAATATCTTGGCTATGATGAGTGCTGTACTTAGATT
TAATTGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCTGGTTTCTCTTTCTCTAC
CTTATGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAAAGTNAAAAACAATGGCC
ATATGCTTCTTCATACCATGCTCACAATATTGTTTTATTCT
sequence update 2001.11.22
Translated Amino Acid sequence 
EKISNFTIDSLQSLWHGSAFFRPLIRSILLKKDYVRNFVSELNNQHRLRFLNTEALAMAQ
YQVLMMGRQELPWDEISSGNVRIDFDTCDGPIVSPKCTYLRRRKPPIPHNKAGNINNALF
NESTKADYEFLGLLDADQQPHPDFLKRVLPYFYSDEGQDLAFVQTPQFFSNIY---

---ASPLIMSIWTVPIVVKDPIIFILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLW
VEATDLWRAEQTFFGFAGTYISSWREGSASIVKLLKARKISRHKLAMWNWKRDFVKKPVV
CEVFRQTKLVNENDNAQESSGKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMM
SAVLRFNCFQNDMWLLVVVAGFSFSTLWHLWSFIPMALRQSXKQWPYASSYHAHNIVLF
Translated Amino Acid sequence (All Frames) 
Frame A:
vkrfptlpltpsnhygmvqhssvh*fvqfs*rkimletlfpn*iinivldssilkh*qwh
nikc**wvdksyhgmkslpvmyv*isilvmvq*fhqnvptfvdvnhpfhiikqvtsimps
lmnqlkpitsslvylmqinnliqisskefyhtftvmkvkiwllfkhhnslvtfi---

---ASPLIMSIWTVPIVVKDPIIFILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLW
VEATDLWRAEQTFFGFAGTYISSWREGSASIVKLLKARKISRHKLAMWNWKRDFVKKPVV
CEVFRQTKLVNENDNAQESSGKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMM
SAVLRFNCFQNDMWLLVVVAGFSFSTLWHLWSFIPMALRQSXKQWPYASSYHAHNIVLF

Frame B:
*kdfqlyh*lppiimawfsilpsidsfnslkerlc*klcfrie*stss*ipqy*sisngt
issvddg*trvtmg*nlfr*ctyrfryl*wsnsftkmylps*t*tthst**sr*hq*cpl
**in*srlrvpwft*crsttssrfpqksftillq**rsrfgfcsnttil**hl---

---llh*l*vfglyqsl*kiqsfsfssv*fl*wcyqesfni*fleprdlmkpvrvvhhfg
*kllifgelnklslvllvlifhhgekvplqslnflklerlvvis*pcgigreis*rnq*f
vkfsvklnwsmkmimlknqvvnirlnnlsvlqtknliplkiqdfsfqilssllliswl**
vlyldlivskmicgfwls*lvslslpygiyglsfqwlfvkvknnghmllhtmltilfys

Frame C:
EKISNFTIDSLQSLWHGSAFFRPLIRSILLKKDYVRNFVSELNNQHRLRFLNTEALAMAQ
YQVLMMGRQELPWDEISSGNVRIDFDTCDGPIVSPKCTYLRRRKPPIPHNKAGNINNALF
NESTKADYEFLGLLDADQQPHPDFLKRVLPYFYSDEGQDLAFVQTPQFFSNIY---

---fsinyeyldctnrckrsnhfhsrrydscngvtkshsiyds*sqetl*sr*ewsitlg
rsy*sles*tnflwfcwylyfimerrfrfnr*ts*s*kd*ss*vshveleerfrketssl
*sfpsn*igq*k**csrikw*t*g*tifpyfkqri*yh*kfktfpskyyplcc*ylgyde
cct*i*lfpk*yvafgcrswflflylmafmvfhsngssskxktmaicffipcsqycfi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFG249 (SFG249Q) /CSM/SF/SFG2-C/SFG249Q.Seq.d/                       2395   0.0
SHK418 (SHK418Q) /CSM/SH/SHK4-A/SHK418Q.Seq.d/                       1400   0.0
SHG576 (SHG576Q) /CSM/SH/SHG5-D/SHG576Q.Seq.d/                       1400   0.0
SHG409 (SHG409Q) /CSM/SH/SHG4-A/SHG409Q.Seq.d/                       1400   0.0
SHG402 (SHG402Q) /CSM/SH/SHG4-A/SHG402Q.Seq.d/                       1400   0.0
SHD789 (SHD789Q) /CSM/SH/SHD7-D/SHD789Q.Seq.d/                       1400   0.0
SHD746 (SHD746Q) /CSM/SH/SHD7-B/SHD746Q.Seq.d/                       1400   0.0
SHC648 (SHC648Q) /CSM/SH/SHC6-B/SHC648Q.Seq.d/                       1400   0.0
SHC269 (SHC269Q) /CSM/SH/SHC2-C/SHC269Q.Seq.d/                       1400   0.0
SHC267 (SHC267Q) /CSM/SH/SHC2-C/SHC267Q.Seq.d/                       1400   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF163835|AF163835.1 Dictyostelium discoideum cellulose synthase (dcsA) mRNA, complete cds.  1400   0.0    1
AL845423|AL845423.4 Mouse DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP23-207C2.    40   0.38   7
AC100467|AC100467.1 Mus musculus clone RP23-139I6, LOW-PASS SEQUENCE SAMPLING.    38   1.2    2
AX707915|AX707915.1 Sequence 683 from Patent WO03013536.           36   2.0    7
AX706985|AX706985.1 Sequence 683 from Patent WO03013534.           36   2.0    7
AC002457|AC002457.2 Homo sapiens BAC clone CTB-60P12 from 7q11.2-q22, complete sequence.    36   2.0    7
AP006429|AP006429.1 Lotus corniculatus var. japonicus genomic DNA, chromosome 1, clone:LjT17D07, TM0322.    36   4.3    6
AC125263|AC125263.3 Mus musculus clone RP24-327I4, WORKING DRAFT SEQUENCE, 7 unordered pieces.    44   4.5    1
AC125647|AC125647.5 Rattus norvegicus clone CH230-231J3, WORKING DRAFT SEQUENCE, 2 unordered pieces.    44   4.5    1
AC090647|AC090647.6 Genomic sequence for Mus musculus, clone RP23-252I12, complete sequence.    44   4.5    1
dna update 2003.12.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9U720) RecName: Full=Cellulose synthase catalytic subunit A [U...   478   e-133
EF563995_1(EF563995|pid:none) Phytophthora infestans cellulose s...    80   2e-13
EF563999_1(EF563999|pid:none) Phytophthora sojae cellulose synth...    80   2e-13
CP000951_2081(CP000951|pid:none) Synechococcus sp. PCC 7002, com...    70   1e-10
EF564002_1(EF564002|pid:none) Phytophthora ramorum cellulose syn...    66   2e-09
EF563993_1(EF563993|pid:none) Phytophthora infestans cellulose s...    66   3e-09
EF564001_1(EF564001|pid:none) Phytophthora ramorum cellulose syn...    65   5e-09
EF563994_1(EF563994|pid:none) Phytophthora infestans cellulose s...    65   6e-09
CP000828_4747(CP000828|pid:none) Acaryochloris marina MBIC11017,...    64   1e-08
EF564004_1(EF564004|pid:none) Phytophthora ramorum cellulose syn...    63   2e-08
protein update 2009. 5.24
PSORT 

 psg: 0.75  gvh: 0.47  alm: 0.23  top: 0.60  tms: 0.27  mit: 0.27  mip: 0.00
 nuc: 0.07  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 36.0 %: plasma membrane
	 16.0 %: vacuolar
	 16.0 %: nuclear
	 16.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for SFG249 is pla
5' end seq. ID SFG249F
5' end seq. 
>SFG249F.Seq
GTGAAAAGATTTCCAACTTTACCATTGACTCCCTCCAATCATTATGGCATGGTTCAGCAT
TCTTCCGTCCATTGATTCGTTCAATTCTCTTAAAGAAAGATTATGTTAGAAACTTTGTTT
CCGAATTGAATAATCAACATCGTCTTAGATTCCTCAATACTGAAGCATTAGCAATGGCAC
AATATCAAGTGTTGATGATGGGTAGACAAGAGTTACCATGGGATGAAATCTCTTCCGGTA
ATGTACGTATAGATTTCGATACTTGTGATGGTCCAATAGTTTCACCAAAATGTACCTACC
TTCGTAGACGTAAACCACCCATTCCACATAATAAAGCAGGTAACATCAATAATGCCCTCT
TTAATGAATCAACTAAAGCCGATTACGAGTTCCTTGGTTTACTTGATGCAGATCAACAAC
CTCATCCAGATTTCCTCAAAAGAGTTTTACCATACTTTTACAGTGATGAAGGTCAAGATT
TGGCTTTTGTTCAAACACCACAATTCTTTAGTAACATTTATCNNNNNNNNNN
Length of 5' end seq. 532
3' end seq. ID SFG249Z
3' end seq. 
>SFG249Z.Seq
----------GCTTCTCCATTAATTATGAGTATTTGGACTGTACCAATCGTTGTAAAAGA
TCCAATCATTTTCATTCTCGTCGGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTCA
ATATATGATTCTTAGAGCCAAGAGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTTG
GGTAGAAGCTACTGATCTTTGGAGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTTA
TATTTCATCATGGAGAGAAGGTTCCGCTTCAATCGTTAAACTTCTTAAAGCTAGAAAGAT
TAGTCGTCATAAGTTAGCCATGTGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGT
TTGTGAAGTTTTCCGTCAAACTAAATTGGTCAATGAAAATGATAATGCTCAAGAATCAAG
TGGTAAACATAAGGCTGAACAATCTTTCCGTACTTCAAACAAAGAATCTGATACCATTAA
AAATTCAAGACTTTTCCTTCCAAATATTATCCTCTTTGTTGTTAATATCTTGGCTATGAT
GAGTGCTGTACTTAGATTTAATTGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAGC
TGGTTTCTCTTTCTCTACCTTATGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTCA
AAGTNAAAAACAATGGCCATATGCTTCTTCATACCATGCTCACAATATTGTTTTATTCT
Length of 3' end seq. 709
Connected seq. ID SFG249P
Connected seq. 
>SFG249P.Seq
GTGAAAAGATTTCCAACTTTACCATTGACTCCCTCCAATCATTATGGCATGGTTCAGCAT
TCTTCCGTCCATTGATTCGTTCAATTCTCTTAAAGAAAGATTATGTTAGAAACTTTGTTT
CCGAATTGAATAATCAACATCGTCTTAGATTCCTCAATACTGAAGCATTAGCAATGGCAC
AATATCAAGTGTTGATGATGGGTAGACAAGAGTTACCATGGGATGAAATCTCTTCCGGTA
ATGTACGTATAGATTTCGATACTTGTGATGGTCCAATAGTTTCACCAAAATGTACCTACC
TTCGTAGACGTAAACCACCCATTCCACATAATAAAGCAGGTAACATCAATAATGCCCTCT
TTAATGAATCAACTAAAGCCGATTACGAGTTCCTTGGTTTACTTGATGCAGATCAACAAC
CTCATCCAGATTTCCTCAAAAGAGTTTTACCATACTTTTACAGTGATGAAGGTCAAGATT
TGGCTTTTGTTCAAACACCACAATTCTTTAGTAACATTTATC----------GCTTCTCC
ATTAATTATGAGTATTTGGACTGTACCAATCGTTGTAAAAGATCCAATCATTTTCATTCT
CGTCGGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTCAATATATGATTCTTAGAGC
CAAGAGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGGGTAGAAGCTACTGATCT
TTGGAGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTTATATTTCATCATGGAGAGA
AGGTTCCGCTTCAATCGTTAAACTTCTTAAAGCTAGAAAGATTAGTCGTCATAAGTTAGC
CATGTGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTTTGTGAAGTTTTCCGTCA
AACTAAATTGGTCAATGAAAATGATAATGCTCAAGAATCAAGTGGTAAACATAAGGCTGA
ACAATCTTTCCGTACTTCAAACAAAGAATCTGATACCATTAAAAATTCAAGACTTTTCCT
TCCAAATATTATCCTCTTTGTTGTTAATATCTTGGCTATGATGAGTGCTGTACTTAGATT
TAATTGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCTGGTTTCTCTTTCTCTAC
CTTATGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAAAGTNAAAAACAATGGCC
ATATGCTTCTTCATACCATGCTCACAATATTGTTTTATTCT
Length of connected seq. 1231
Full length Seq ID -
Full length Seq. -
Length of full length seq. -