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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFG176
Library SF
(Link to library)
Clone ID SFG176
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15330-1
Original site URL
Representative seq. ID SFG176P
(Link to Original site)
Representative DNA sequence 
>SFG176 (SFG176Q) /CSM/SF/SFG1-D/SFG176Q.Seq.d/
AATATATATATTTTTTAATTAATAAAAAATCATATATATATCTTATAAATTGGCTTNCAT
ACAAATTAAAACCGTTATTATTAAATAACTTACATCATATAAATTTATNCTTTTAGTATA
TCTTTTTCACTCTCATCTTTTTTTATTCCCCATTTAAAAAATXXXXXXXXXXATTAGAAG
AATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAATTAAATATAGTAAAGATAC
TAGATATAACGATAACATTGATAAATCATTTTTAGTTACCCATGACAAACAAAATTATCA
AGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGANCAAGCACAAAACTATGATGTAAT
TGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAATTTAGTGAAGACTTGGCAAA
TCAAGGTAAAGCCGTTATAATTGCCGCACTAGATGGTACCTTCCAAAGAAAACCATTCCA
ATCAGTCATTGATTTAGTATCAAAGGCTGAATATATTACAAAGTTAACTGCCGTTTGTAT
GGTTTGCTACAATGAAGCCGCCTTTTCAAAACGTATCGTCGAAAGTGATGATATCGAATT
AATTGGTGGTATAGATAAATATATTTCCGTTTG
sequence update 2001.11.22
Translated Amino Acid sequence 
niyif*liknhiyil*igxhtn*nryy*ityii*IYXFSISFSLSSFFIPHLKN---

---IRRIKRFNFANKKCLLIKYSKDTRYNDNIDKSFLVTHDKQNYQAFPCSILEDVKXQA
QNYDVIGIDEGQFFPDVVQFSEDLANQGKAVIIAALDGTFQRKPFQSVIDLVSKAEYITK
LTAVCMVCYNEAAFSKRIVESDDIELIGGIDKYISV
Translated Amino Acid sequence (All Frames) 
Frame A:
niyif*liknhiyil*igxhtn*nryy*ityii*IYXFSISFSLSSFFIPHLKN---

---IRRIKRFNFANKKCLLIKYSKDTRYNDNIDKSFLVTHDKQNYQAFPCSILEDVKXQA
QNYDVIGIDEGQFFPDVVQFSEDLANQGKAVIIAALDGTFQRKPFQSVIDLVSKAEYITK
LTAVCMVCYNEAAFSKRIVESDDIELIGGIDKYISV

Frame B:
iyiffn**kiiyisyklaxiqiktviik*ltsykfxllvylfhshlflfpi*k---

---leelkdlilqtknay*lnivkildititlinhf*lpmtnkiiklshvqf*kmlkxkh
ktmm*lvlmkanfsqtlynlvktwqikvkpl*lph*mvpskenhsnqsli*yqrlnilqs
*lpfvwfatmkppfqnvsskvmisn*lvv*inifpf

Frame C:
yiyflinkksyiylinwlxyklkplllnnlhhinlxf*yifftliffyspfkk---

---*kn*ki*fckqkmlin*i**ry*i*r*h**iifsyp*qtklssfpmfnfrrc*rxst
kl*cnwy**rpifprrcti**rlgksr*sryncrtrwylpkktipish*fsikg*iyykv
ncrlygllq*srlfktyrrk**yrinwwyr*iyfrl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFG176 (SFG176Q) /CSM/SF/SFG1-D/SFG176Q.Seq.d/                       1037   0.0
VFE258 (VFE258Q) /CSM/VF/VFE2-C/VFE258Q.Seq.d/                        902   0.0
VSJ693 (VSJ693Q) /CSM/VS/VSJ6-D/VSJ693Q.Seq.d/                        900   0.0
VHQ109 (VHQ109Q) /CSM/VH/VHQ1-A/VHQ109Q.Seq.d/                        900   0.0
SSI374 (SSI374Q) /CSM/SS/SSI3-D/SSI374Q.Seq.d/                        900   0.0
SSF620 (SSF620Q) /CSM/SS/SSF6-A/SSF620Q.Seq.d/                        900   0.0
CFJ604 (CFJ604Q) /CSM/CF/CFJ6-A/CFJ604Q.Seq.d/                        900   0.0
VFL501 (VFL501Q) /CSM/VF/VFL5-A/VFL501Q.Seq.d/                        898   0.0
VFC738 (VFC738Q) /CSM/VF/VFC7-B/VFC738Q.Seq.d/                        898   0.0
SLH838 (SLH838Q) /CSM/SL/SLH8-B/SLH838Q.Seq.d/                        894   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U61990|U61990.1 Dictyostelium discoideum ThyB (thyB) mRNA, complete cds.   900   0.0    3
AF510846|AF510846.1 Dictyostelium discoideum calmodulin-binding protein ThyB (thyB) mRNA, complete cds.   900   0.0    3
CB888459|CB888459.1 taa92g08.x1 Hydra EST -III Hydra magnipapillata cDNA 3' similar to SW:KITH_CHICK P04047 THYMIDINE KINASE, CYTOSOLIC ;, mRNA sequence.    56   6e-06  2
AP003193|AP003193.2 Clostridium perfringens DNA, complete genome, section 9/10.    36   3e-04  12
AE014829|AE014829.1 Plasmodium falciparum 3D7 chromosome 10 section 1 of 7 of the complete sequence.    38   0.006  11
AE014818|AE014818.1 Plasmodium falciparum 3D7 chromosome 14 section 3 of 13 of the complete sequence.    32   0.006  13
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    32   0.006  13
AZ692494|AZ692494.1 ENTJU18TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   0.007  2
CB823537|CB823537.1 EST 4761 Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bh006p21 3', mRNA sequence.    50   0.037  1
CB823884|CB823884.1 EST 5108 Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bh002g05 3', mRNA sequence.    50   0.037  1
dna update 2003.12.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04183) RecName: Full=Thymidine kinase, cytosolic;          EC=...   175   8e-43
AY335565_1(AY335565|pid:none) Synthetic construct Homo sapiens t...   175   8e-43
AM905441_1(AM905441|pid:none) Suberites domuncula mRNA for thymi...   175   1e-42
BT030694_1(BT030694|pid:none) Bos taurus thymidine kinase 1, sol...   174   1e-42
K02581_1(K02581|pid:none) Human thymidine kinase mRNA, complete ...   174   2e-42
AB451268_1(AB451268|pid:none) Homo sapiens TK1 mRNA for thymidin...   174   2e-42
(P09768) RecName: Full=Thymidine kinase, cytosolic;          EC=...   172   9e-42
DQ441416_85(DQ441416|pid:none) Variola virus strain Benin, Dahom...   171   1e-41
(P04364) RecName: Full=Thymidine kinase;          EC=2.7.1.21;  ...   171   1e-41
DQ206438_1(DQ206438|pid:none) Guarani P1 virus thymidine kinase ...   171   2e-41
protein update 2009. 5.24
PSORT 

 psg: 0.95  gvh: 0.38  alm: 0.44  top: 0.83  tms: 0.00  mit: 0.43  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 28.0 %: nuclear
	 24.0 %: cytoplasmic
	  4.0 %: peroxisomal

>> prediction for SFG176 is mit
5' end seq. ID SFG176F
5' end seq. 
>SFG176F.Seq
AATATATATATTTTTTAATTAATAAAAAATCATATATATATCTTATAAATTGGCTTNCAT
ACAAATTAAAACCGTTATTATTAAATAACTTACATCATATAAATTTATNCTTTTAGTATA
TCTTTTTCACTCTCATCTTTTTTTATTCCCCATTTAAAAAATNNNNNNNNNN
Length of 5' end seq. 172
3' end seq. ID SFG176Z
3' end seq. 
>SFG176Z.Seq
----------ATTAGAAGAATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAAT
TAAATATAGTAAAGATACTAGATATAACGATAACATTGATAAATCATTTTTAGTTACCCA
TGACAAACAAAATTATCAAGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGANCAAGC
ACAAAACTATGATGTAATTGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAATT
TAGTGAAGACTTGGCAAATCAAGGTAAAGCCGTTATAATTGCCGCACTAGATGGTACCTT
CCAAAGAAAACCATTCCAATCAGTCATTGATTTAGTATCAAAGGCTGAATATATTACAAA
GTTAACTGCCGTTTGTATGGTTTGCTACAATGAAGCCGCCTTTTCAAAACGTATCGTCGA
AAGTGATGATATCGAATTAATTGGTGGTATAGATAAATATATTTCCGTTTG
Length of 3' end seq. 461
Connected seq. ID SFG176P
Connected seq. 
>SFG176P.Seq
AATATATATATTTTTTAATTAATAAAAAATCATATATATATCTTATAAATTGGCTTNCAT
ACAAATTAAAACCGTTATTATTAAATAACTTACATCATATAAATTTATNCTTTTAGTATA
TCTTTTTCACTCTCATCTTTTTTTATTCCCCATTTAAAAAAT----------ATTAGAAG
AATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAATTAAATATAGTAAAGATAC
TAGATATAACGATAACATTGATAAATCATTTTTAGTTACCCATGACAAACAAAATTATCA
AGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGANCAAGCACAAAACTATGATGTAAT
TGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAATTTAGTGAAGACTTGGCAAA
TCAAGGTAAAGCCGTTATAATTGCCGCACTAGATGGTACCTTCCAAAGAAAACCATTCCA
ATCAGTCATTGATTTAGTATCAAAGGCTGAATATATTACAAAGTTAACTGCCGTTTGTAT
GGTTTGCTACAATGAAGCCGCCTTTTCAAAACGTATCGTCGAAAGTGATGATATCGAATT
AATTGGTGGTATAGATAAATATATTTCCGTTTG
Length of connected seq. 623
Full length Seq ID -
Full length Seq. -
Length of full length seq. -