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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFF774
Library SF
(Link to library)
Clone ID SFF774
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U00735-1|Contig-U15639-1
Original site URL
Representative seq. ID SFF774P
(Link to Original site)
Representative DNA sequence 
>SFF774 (SFF774Q) /CSM/SF/SFF7-D/SFF774Q.Seq.d/
GTCGTATCATTTCATGTATTCAAGAGGTTGAATGGAAGATGTGGAGATTAGGCATGCCAT
TGAAAACCAGACATAATGAAGTCGCACCAGGTCAATATGAAGTCGCACCAATCTTTGAAC
GTGCTAATATTGCAGCCGATCATAATATGATGTTGATGGATATTTTAAAGAATGTTTCAA
CTAAACATGGTTTGGTTTGTCTTTTCCATGAGAAACCATTTGCTGGTGTCAATGGTTCTG
GTAAACATAACAATTGGTCCCTTTCAACCGATGGTGGTAGTAATCTTTTGGAGCCAGGTC
ATACACCATCACAAAATGCACGTTTCATTCTTTTCCTTACAGCAATTATTAGAGCAGTCG
ATATTCATGCTGATCTTCTTCGTGCATCAGTCGCAGTACCAGGTAATGAGCATCGTTTAG
GTGCCAATGAAGCACCACCAGCAATCATTTCAATCTATCTCGGTAAGGAACTTGATACCG
TGATTAACAATATCATTAACAATACCGATATTCAAGCACCAGGTTCAGATGATATGGATT
TGGGTGTCGTTGGGTTCCCAXXXXXXXXXXAGTTAGTAGTAGACAAGCTGCATTCAATAN
AATCATTAAAGATACATTGATTCAACATGTTCGTGTTGTTTTCAATGGTGATGGTTATAG
TGGTGATTGGAAAGAACTCGCCAAATCTAGAGGTTTAGCAAATTTACCATCGACTCCAGA
GGCTTTAACAAATATTAACTCTGAAAAGAATATTAAACTCTTTTCTGAAAGTAATATTCT
CTCACCAGTTGAATTGGAATCACGTCAAGAGATTCTCTTTGNAATTTATAATAAATCAAT
TANAATCGAAGCAAACTCTTTATATGATTTAGTTTCAACTTTGGTACTTCCAGCTTGTTT
TGCTCATCAAAAGAATATTGCTGAATCTGTAAACTCTATAATGCCATTCATTCAAAGTCA
AAAATCTTTCAGTCAACCAAATCATCAATATTCTCATCTCTCTGAAGTCGTTGAATCTGT
CAATCTCTTAATTGAAGCTAATCAAAAACTTTTGGCTCTCATTAAACAAACTAAAGATTT
CAATTCTGAACATTCACTCGCAACTTTCCTCAATCAATCTGTAATTCCACAAATGAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
RIISCIQEVEWKMWRLGMPLKTRHNEVAPGQYEVAPIFERANIAADHNMMLMDILKNVST
KHGLVCLFHEKPFAGVNGSGKHNNWSLSTDGGSNLLEPGHTPSQNARFILFLTAIIRAVD
IHADLLRASVAVPGNEHRLGANEAPPAIISIYLGKELDTVINNIINNTDIQAPGSDDMDL
GVVGFP---

---VSSRQAAFNXIIKDTLIQHVRVVFNGDGYSGDWKELAKSRGLANLPSTPEALTNINS
EKNIKLFSESNILSPVELESRQEILFXIYNKSIXIEANSLYDLVSTLVLPACFAHQKNIA
ESVNSIMPFIQSQKSFSQPNHQYSHLSEVVESVNLLIEANQKLLALIKQTKDFNSEHSLA
TFLNQSVIPQM
Translated Amino Acid sequence (All Frames) 
Frame A:
vvsfhvfkrlngrcgd*ach*kpdimkshqvnmkshqslnvlilqpiii*c*wif*rmfq
lnmvwfvfsmrnhllvsmvlvnitigpfqpmvvvifwsqvihhhkmhvsffslqqlleqs
ifmliffvhqsqyqvmsiv*vpmkhhqqsfqsisvrnlip*ltisltipifkhqvqmiwi
wvslgs---

---s***tsciqxnh*ryidstcsccfqw*wl*w*lertrqi*rfskftidsrgfnky*l
*key*tlf*k*yslts*igitsrdslxnl**inxnrsklfi*fsfnfgtsslfcsskeyc
*icklynaihskskifqstkssifssl*sr*icqsln*s*sktfgsh*tn*rfqf*tftr
nfpqsicnstne

Frame B:
syhfmysrg*medveirhaienqt**srtrsi*srtnl*tc*ycsrs*ydvdgyfkecfn
*twfglsfp*eticwcqwfw*t*qlvpfnrww**sfgarsytitkctfhsfpysny*ssr
ysc*ssscisrstr**asfrcq*sttsnhfnlsr*gt*yrd*qyh*qyrysstrfr*ygf
gcrwvp---

---VSSRQAAFNXIIKDTLIQHVRVVFNGDGYSGDWKELAKSRGLANLPSTPEALTNINS
EKNIKLFSESNILSPVELESRQEILFXIYNKSIXIEANSLYDLVSTLVLPACFAHQKNIA
ESVNSIMPFIQSQKSFSQPNHQYSHLSEVVESVNLLIEANQKLLALIKQTKDFNSEHSLA
TFLNQSVIPQM

Frame C:
RIISCIQEVEWKMWRLGMPLKTRHNEVAPGQYEVAPIFERANIAADHNMMLMDILKNVST
KHGLVCLFHEKPFAGVNGSGKHNNWSLSTDGGSNLLEPGHTPSQNARFILFLTAIIRAVD
IHADLLRASVAVPGNEHRLGANEAPPAIISIYLGKELDTVINNIINNTDIQAPGSDDMDL
GVVGFP---

---lvvdklhsixslkih*fnmfvlfsmvmvivvigknspnlev*qiyhrlqrl*qiltl
krilnsflkvifshqlnwnhvkrfslxfiinqlxskqtlymi*fqlwyfqlvllikrill
nl*tl*chsfkvknlsvnqiinilislkslnlsis*lkliknfwlslnklkisilnihsq
lssinl*fhk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFF774 (SFF774Q) /CSM/SF/SFF7-D/SFF774Q.Seq.d/                       2177   0.0
SHK163 (SHK163Q) /CSM/SH/SHK1-C/SHK163Q.Seq.d/                       1100   0.0
VHG867 (VHG867Q) /CSM/VH/VHG8-C/VHG867Q.Seq.d/                       1098   0.0
SHJ542 (SHJ542Q) /CSM/SH/SHJ5-B/SHJ542Q.Seq.d/                       1098   0.0
SHA796 (SHA796Q) /CSM/SH/SHA7-D/SHA796Q.Seq.d/                       1098   0.0
SHA735 (SHA735Q) /CSM/SH/SHA7-B/SHA735Q.Seq.d/                       1098   0.0
CHS409 (CHS409Q) /CSM/CH/CHS4-A/CHS409Q.Seq.d/                       1098   0.0
CHR354 (CHR354Q) /CSM/CH/CHR3-C/CHR354Q.Seq.d/                       1098   0.0
CHQ879 (CHQ879Q) /CSM/CH/CHQ8-D/CHQ879Q.Seq.d/                       1098   0.0
CHQ559 (CHQ559Q) /CSM/CH/CHQ5-C/CHQ559Q.Seq.d/                       1098   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BH153298|BH153298.1 ENTSD82TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, genomic survey sequence.    50   2e-06  2
AF483911|AF483911.1 Prevotella bryantii strain B14 type III glutamine synthetase gene, complete cds.    58   3e-04  1
AF280689|AF280689.1 Bacteroides fragilis isolate BFR 2393 glutamine synthetase (glnA) gene, partial cds.    56   3e-04  2
AF280688|AF280688.1 Bacteroides fragilis isolate Tal2480 glutamine synthetase (glnA) gene, partial cds.    56   3e-04  2
AF280643|AF280643.1 Bacteroides fragilis isolate BFR3392 glutamine synthetase (glnA) gene, partial cds.    56   3e-04  2
AF280679|AF280679.1 Bacteroides fragilis isolate 1297 glutamine synthetase (glnA) gene, partial cds.    56   3e-04  2
AF280641|AF280641.1 Bacteroides fragilis isolate Tal3636 glutamine synthetase (glnA) gene, partial cds.    56   3e-04  2
AF280644|AF280644.1 Bacteroides fragilis isolate BFR81R glutamine synthetase (glnA) gene, partial cds.    56   3e-04  2
AF280650|AF280650.1 Bacteroides fragilis isolate 28794 glutamine synthetase (glnA) gene, partial cds.    56   3e-04  2
AF280656|AF280656.1 Bacteroides fragilis isolate 1429 glutamine synthetase (glnA) gene, partial cds.    56   3e-04  2
dna update 2003.12.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54WR9) RecName: Full=Type-3 glutamine synthetase;          EC=...   365   2e-99
CP001124_3048(CP001124|pid:none) Geobacter bemidjiensis Bem, com...   232   2e-59
CP000568_195(CP000568|pid:none) Clostridium thermocellum ATCC 27...   227   5e-58
CP000252_3065(CP000252|pid:none) Syntrophus aciditrophicus SB, c...   226   8e-58
CP001071_1230(CP001071|pid:none) Akkermansia muciniphila ATCC BA...   226   8e-58
CP000885_3336(CP000885|pid:none) Clostridium phytofermentans ISD...   225   2e-57
CP001114_1380(CP001114|pid:none) Deinococcus deserti VCD115, com...   224   5e-57
CP000568_1524(CP000568|pid:none) Clostridium thermocellum ATCC 2...   224   5e-57
CP000359_1193(CP000359|pid:none) Deinococcus geothermalis DSM 11...   221   3e-56
CP001348_555(CP001348|pid:none) Clostridium cellulolyticum H10, ...   221   4e-56
protein update 2009. 5.24
PSORT 

 psg: 0.75  gvh: 0.29  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal

>> prediction for SFF774 is cyt
5' end seq. ID SFF774F
5' end seq. 
>SFF774F.Seq
GTCGTATCATTTCATGTATTCAAGAGGTTGAATGGAAGATGTGGAGATTAGGCATGCCAT
TGAAAACCAGACATAATGAAGTCGCACCAGGTCAATATGAAGTCGCACCAATCTTTGAAC
GTGCTAATATTGCAGCCGATCATAATATGATGTTGATGGATATTTTAAAGAATGTTTCAA
CTAAACATGGTTTGGTTTGTCTTTTCCATGAGAAACCATTTGCTGGTGTCAATGGTTCTG
GTAAACATAACAATTGGTCCCTTTCAACCGATGGTGGTAGTAATCTTTTGGAGCCAGGTC
ATACACCATCACAAAATGCACGTTTCATTCTTTTCCTTACAGCAATTATTAGAGCAGTCG
ATATTCATGCTGATCTTCTTCGTGCATCAGTCGCAGTACCAGGTAATGAGCATCGTTTAG
GTGCCAATGAAGCACCACCAGCAATCATTTCAATCTATCTCGGTAAGGAACTTGATACCG
TGATTAACAATATCATTAACAATACCGATATTCAAGCACCAGGTTCAGATGATATGGATT
TGGGTGTCGTTGGGTTCCCA----------
Length of 5' end seq. 560
3' end seq. ID SFF774Z
3' end seq. 
>SFF774Z.Seq
----------AGTTAGTAGTAGACAAGCTGCATTCAATANAATCATTAAAGATACATTGA
TTCAACATGTTCGTGTTGTTTTCAATGGTGATGGTTATAGTGGTGATTGGAAAGAACTCG
CCAAATCTAGAGGTTTAGCAAATTTACCATCGACTCCAGAGGCTTTAACAAATATTAACT
CTGAAAAGAATATTAAACTCTTTTCTGAAAGTAATATTCTCTCACCAGTTGAATTGGAAT
CACGTCAAGAGATTCTCTTTGNAATTTATAATAAATCAATTANAATCGAAGCAAACTCTT
TATATGATTTAGTTTCAACTTTGGTACTTCCAGCTTGTTTTGCTCATCAAAAGAATATTG
CTGAATCTGTAAACTCTATAATGCCATTCATTCAAAGTCAAAAATCTTTCAGTCAACCAA
ATCATCAATATTCTCATCTCTCTGAAGTCGTTGAATCTGTCAATCTCTTAATTGAAGCTA
ATCAAAAACTTTTGGCTCTCATTAAACAAACTAAAGATTTCAATTCTGAACATTCACTCG
CAACTTTCCTCAATCAATCTGTAATTCCACAAATGAA
Length of 3' end seq. 567
Connected seq. ID SFF774P
Connected seq. 
>SFF774P.Seq
GTCGTATCATTTCATGTATTCAAGAGGTTGAATGGAAGATGTGGAGATTAGGCATGCCAT
TGAAAACCAGACATAATGAAGTCGCACCAGGTCAATATGAAGTCGCACCAATCTTTGAAC
GTGCTAATATTGCAGCCGATCATAATATGATGTTGATGGATATTTTAAAGAATGTTTCAA
CTAAACATGGTTTGGTTTGTCTTTTCCATGAGAAACCATTTGCTGGTGTCAATGGTTCTG
GTAAACATAACAATTGGTCCCTTTCAACCGATGGTGGTAGTAATCTTTTGGAGCCAGGTC
ATACACCATCACAAAATGCACGTTTCATTCTTTTCCTTACAGCAATTATTAGAGCAGTCG
ATATTCATGCTGATCTTCTTCGTGCATCAGTCGCAGTACCAGGTAATGAGCATCGTTTAG
GTGCCAATGAAGCACCACCAGCAATCATTTCAATCTATCTCGGTAAGGAACTTGATACCG
TGATTAACAATATCATTAACAATACCGATATTCAAGCACCAGGTTCAGATGATATGGATT
TGGGTGTCGTTGGGTTCCCA----------AGTTAGTAGTAGACAAGCTGCATTCAATAN
AATCATTAAAGATACATTGATTCAACATGTTCGTGTTGTTTTCAATGGTGATGGTTATAG
TGGTGATTGGAAAGAACTCGCCAAATCTAGAGGTTTAGCAAATTTACCATCGACTCCAGA
GGCTTTAACAAATATTAACTCTGAAAAGAATATTAAACTCTTTTCTGAAAGTAATATTCT
CTCACCAGTTGAATTGGAATCACGTCAAGAGATTCTCTTTGNAATTTATAATAAATCAAT
TANAATCGAAGCAAACTCTTTATATGATTTAGTTTCAACTTTGGTACTTCCAGCTTGTTT
TGCTCATCAAAAGAATATTGCTGAATCTGTAAACTCTATAATGCCATTCATTCAAAGTCA
AAAATCTTTCAGTCAACCAAATCATCAATATTCTCATCTCTCTGAAGTCGTTGAATCTGT
CAATCTCTTAATTGAAGCTAATCAAAAACTTTTGGCTCTCATTAAACAAACTAAAGATTT
CAATTCTGAACATTCACTCGCAACTTTCCTCAATCAATCTGTAATTCCACAAATGAA
Length of connected seq. 1127
Full length Seq ID -
Full length Seq. -
Length of full length seq. -