>AFI278 (AFI278Q) /CSM/AF/AFI2-D/AFI278Q.Seq.d/
ACTGTTGGCCTACTGGAATTACAATTACTAACCTATTATTACTATTACTATTTATTATTT
ATTATTATTTATATTAAATTATGTATTACAGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAAC
ATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAAC
ATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCAT
TGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTT
ATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATA
TGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGATGG
XXXXXXXXXXCTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTG
TAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCG
CTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATC
ATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATC
TCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTG
AAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTA
CTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATC
AAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAA
TTGTAAAAGAAGTTTGTAAAGAACATNGCATTACTTACCACATTAAACCAAACTTCACTG
NAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGCATTATGTT
AAAAAACCATTAGCCTCAAAAGA

cwptgititnllllllfiiyyyly*IMYYSNKMSKVITGKQYSWSELAKHNTENDCWVAV
DGKVYDITRWVPLHPGGKEVLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEH
PKFVQKSDFYKTLKERVRKHFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFY
LNCFLAIVYALCNSLFSMHMMHDSCHAAISHYPGVWKWM---

---YGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYH
SFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTT
QDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHXITYHIKPNFTX
AIMSHINYLYKMGNDPALC*ktislkr

Frame A:
tvgllelqlltyyyyyyllfiiiyiklcitvikcqk*lqesntlgqs*pnitlkmivglq
smvkfmilqdgyhyiqvvkkfyf*qqvemlqiylkviiqcqinqhqf*rimkldifhhmn
iqnlfknqistrh*krelenilkqpikihkwqlvfshv*hwfif*cl*lti*hiipvtif
i*ivswqsfthfathysqci*cmihvmlpfhiiqaygngw---

---lwylhtqisysrlgsfckgw*kwcnsc*crhkfr*grlrhw*ivfcflpfhpstpls
*lyrfnllfphc*irlwlvshn*fps*scr*rsqilcyp*ktr*tisnq*rlgnpst*ny
srlwswftplylf*wffkssscssfipincsrflpttctnckrsl*rtxhylph*tklhx
syyvth*lplqng**ssimlknh*pqk

Frame B:
llaywnyny*piititiyylllfilnyvlq**nvksnyrkaillvrvsqt*h*k*llgcs
rw*sl*yykmgtitsrw*rsftfssr*rcykfi*klssnvr*tninfkel*nwiyfii*t
skicskirflqdikres*ktf*snrsrstngs*yfltfsiglsfsvcnllfstlyq*qfl
fklflgnrlrtlqliilnayda*fmscchftlsrrmemd---

---YGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYH
SFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTT
QDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHXITYHIKPNFTX
AIMSHINYLYKMGNDPALC*ktislkr

Frame C:
cwptgititnllllllfiiyyyly*IMYYSNKMSKVITGKQYSWSELAKHNTENDCWVAV
DGKVYDITRWVPLHPGGKEVLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEH
PKFVQKSDFYKTLKERVRKHFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFY
LNCFLAIVYALCNSLFSMHMMHDSCHAAISHYPGVWKWM---

---mvfthsnivlkigkll*rmvkmvqfvlvspqisirpltslvncllfssvssfhsvii
alqi*fvissllnsslvgisqlisklvmslkisnsllplkdqmnhlksmkigqsfnlkll
kimvmvhsfvpflvvl*iiklfiiyshqllkisthnlyql*kkfvknxallttlnqtslx
llchtlitftkwvmiqhyvkkplask

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI278 (AFI278Q) /CSM/AF/AFI2-D/AFI278Q.Seq.d/                       2298   0.0
VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1179   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1179   0.0
VFI562 (VFI562Q) /CSM/VF/VFI5-C/VFI562Q.Seq.d/                       1179   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1179   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1179   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1179   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1179   0.0
CFC256 (CFC256Q) /CSM/CF/CFC2-C/CFC256Q.Seq.d/                       1179   0.0
VSH813 (VSH813Q) /CSM/VS/VSH8-A/VSH813Q.Seq.d/                       1166   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BD092935|BD092935.1 Methods and compositions for synthesis of longfatty acids in plants.  1132   0.0    2
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1132   0.0    2
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1132   0.0    2
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.   902   0.0    5
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.   149   e-101  10
AR271569|AR271569.1 Sequence 1 from patent US 6503729.             32   0.029  25
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    38   0.20   12
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    32   0.23   22
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34   0.32   17
AC129112|AC129112.4 Rattus norvegicus clone CH230-265O9, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    48   0.34   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   714   0.0
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   458   e-127
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   238   4e-61
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   182   2e-44
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    164   6e-39
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   149   2e-34
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   148   4e-34
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   147   1e-33
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   138   4e-31
CS020137_1(CS020137|pid:none) Sequence 91 from Patent WO20050123...   123   1e-26

 psg: 0.70  gvh: 0.24  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFI278 is nuc

>AFI278F.Seq
ACTGTTGGCCTACTGGAATTACAATTACTAACCTATTATTACTATTACTATTTATTATTT
ATTATTATTTATATTAAATTATGTATTACAGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAAC
ATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAAC
ATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCAT
TGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTT
ATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATA
TGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGATGG
----------

>AFI278Z.Seq
----------CTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTG
TAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCG
CTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATC
ATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATC
TCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTG
AAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTA
CTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATC
AAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAA
TTGTAAAAGAAGTTTGTAAAGAACATNGCATTACTTACCACATTAAACCAAACTTCACTG
NAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGCATTATGTT
AAAAAACCATTAGCCTCAAAAGA

>AFI278P.Seq
ACTGTTGGCCTACTGGAATTACAATTACTAACCTATTATTACTATTACTATTTATTATTT
ATTATTATTTATATTAAATTATGTATTACAGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAAC
ATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAAC
ATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCAT
TGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTT
ATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATA
TGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGATGG
----------CTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTG
TAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCG
CTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATC
ATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATC
TCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTG
AAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTA
CTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATC
AAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAA
TTGTAAAAGAAGTTTGTAAAGAACATNGCATTACTTACCACATTAAACCAAACTTCACTG
NAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGCATTATGTT
AAAAAACCATTAGCCTCAAAAGA