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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI273
Library AF
(Link to library)
Clone ID AFI273
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFI273Z
(Link to Original site)
Representative DNA sequence 
>AFI273 (AFI273Q) /CSM/AF/AFI2-D/AFI273Q.Seq.d/
XXXXXXXXXXAGCTCGTTACAGTAAATCACCATACATTTATCCATTATATGGTTTAGGTG
AATTACCACAAGCATTTGCCCGTTTATCTGCTATCTATGGTGGTACATATATGCTTAATA
AACCAATTGAAAATATTGTTTTCGATGAAGAAAAGAAAATTATTAAAGTTACATCTGAAG
GTGAAACTGTAACATGTGATAAAATTGTTTGTGATCCATCATATTTCCCAAATAAAGTTC
AAAAGAAAGGTAAAATTATTCGTTCAATTTGTATTTTGAATGGTCCAATTCCATCTGCTG
ATAAGAAATTTGAATCACTCCAAATCATTATCCCACAAAAACAAGTTAAACGTCAATCAG
ATATGTACGTTGGTGTTATGTCATCCGCTCATTGTGTCTGTCCAAAGGGTAAATACATTG
CCATCGTTTCAGCCACTGTTGAAACCAATGAACCAGAGAAAGAATTAGCACCAGCTTACG
CTTTATTAGGTCCAATTCAAGAGAAATTTGTCTATATTTCTGATTTCATTGAACCAATTG
CTGATGGTACTGCCGACAATGTTTTCATCTCAAAATCATATGATGCTACCTCTCACTTTG
AAACCACTTGTGAAGATGTCA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
---ARYSKSPYIYPLYGLGELPQAFARLSAIYGGTYMLNKPIENIVFDEEKKIIKVTSEG
ETVTCDKIVCDPSYFPNKVQKKGKIIRSICILNGPIPSADKKFESLQIIIPQKQVKRQSD
MYVGVMSSAHCVCPKGKYIAIVSATVETNEPEKELAPAYALLGPIQEKFVYISDFIEPIA
DGTADNVFISKSYDATSHFETTCEDV
Translated Amino Acid sequence (All Frames) 
Frame A:
---ARYSKSPYIYPLYGLGELPQAFARLSAIYGGTYMLNKPIENIVFDEEKKIIKVTSEG
ETVTCDKIVCDPSYFPNKVQKKGKIIRSICILNGPIPSADKKFESLQIIIPQKQVKRQSD
MYVGVMSSAHCVCPKGKYIAIVSATVETNEPEKELAPAYALLGPIQEKFVYISDFIEPIA
DGTADNVFISKSYDATSHFETTCEDV


Frame B:
---lvtvnhhtfihymv*vnyhkhlpvyllsmvvhiclinqlkilfsmkkrkllklhlkv
kl*hviklfvihhisqikfkrkvklfvqfvf*mvqfhllirnlnhskslshknklnvnqi
ctlvlchplivsvqrvntlpsfqpllkpmnqrkn*hqltly*vqfkrnlsiflislnqll
mvlptmfssqnhmmlpltlkplvkms


Frame C:
---slq*itihlsiiwfr*ittsicpficylwwyiya**tn*kycfr*rkeny*syi*r*
ncnm**ncl*siifpk*ssker*nysfnlyfewsnsic**ei*itpnhyptkts*tsiry
vrwcyvirslclskg*ihchrfshc*nq*treristslrfirsnsreiclyf*fh*tnc*
wycrqcfhlkii*cylsl*nhl*rc
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL120 (SFL120Q) /CSM/SF/SFL1-A/SFL120Q.Seq.d/                       1211   0.0
SFJ795 (SFJ795Q) /CSM/SF/SFJ7-D/SFJ795Q.Seq.d/                       1211   0.0
SFJ449 (SFJ449Q) /CSM/SF/SFJ4-C/SFJ449Q.Seq.d/                       1211   0.0
SFI510 (SFI510Q) /CSM/SF/SFI5-A/SFI510Q.Seq.d/                       1211   0.0
SFD219 (SFD219Q) /CSM/SF/SFD2-A/SFD219Q.Seq.d/                       1211   0.0
CHB577 (CHB577Q) /CSM/CH/CHB5-D/CHB577Q.Seq.d/                       1211   0.0
CFJ161 (CFJ161Q) /CSM/CF/CFJ1-C/CFJ161Q.Seq.d/                       1211   0.0
CFH888 (CFH888Q) /CSM/CF/CFH8-D/CFH888Q.Seq.d/                       1211   0.0
CFE844 (CFE844Q) /CSM/CF/CFE8-B/CFE844Q.Seq.d/                       1211   0.0
CFC760 (CFC760Q) /CSM/CF/CFC7-C/CFC760Q.Seq.d/                       1211   0.0
own update 2002. 9.16
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL408803|AL408803.1 T3 end of clone AV0AA010G06 of library AV0AA from strain CBS 379 ofSaccharomyces exiguus.    54   7e-10  3
AJ005836|AJ005836.1 Cicer arietinum mRNA for GDP dissociation inhibitor (gdi).    38   9e-10  4
BG790353|BG790353.1 sae68c10.y1 Gm-c1064 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1064-3212 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    62   3e-08  2
BE800797|BE800797.1 sq98c12.y1 Gm-c1049 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1049-1199 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    62   3e-08  2
AW397479|AW397479.1 sg79d05.y1 Gm-c1007 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1007-2626 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    62   3e-08  2
BU964562|BU964562.1 sas99a12.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-12311 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    62   3e-08  2
BI315670|BI315670.1 saf75f12.y1 Gm-c1078 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1078-2184 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    62   1e-07  2
AI437629|AI437629.1 sa37e09.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1004-1505 5' similar to TR:O24653 O24653 GDI2. ;, mRNA sequence.    62   1e-07  2
CN141329|CN141329.1 OX1_50_F11.g1_A002 Oxidatively-stressed leaves and roots Sorghum bicolor cDNA clone OX1_50_F11_A002 5', mRNA sequence.    68   2e-07  1
BW478676|BW478676.1 Ciona intestinalis cDNA, clone:cima018l21, 5'end, single read.    46   5e-07  4
dna update 2004. 6.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF251042_1(AF251042|pid:none) Mus musculus rab GDP dissociation ...   257   2e-67
AF130987_1(AF130987|pid:none) Rattus norvegicus RAB GDP dissocia...   256   4e-67
(O97555) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   256   4e-67
(P50398) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   256   4e-67
X74402_1(X74402|pid:none) R.norvegicus rab GDI alpha mRNA.            256   4e-67
(P21856) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   255   8e-67
(Q8HXX7) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   254   1e-66
(P31150) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   254   1e-66
A54091(A54091;S35964;A37996)rab GDP dissociation inhibitor alpha...   254   1e-66
AB171705_1(AB171705|pid:none) Macaca fascicularis brain cDNA clo...   254   1e-66
protein update 2009. 4.22
PSORT 

 psg: 0.85  gvh: 0.62  alm: 0.47  top: 0.33  tms: 0.00  mit: 0.30  mip: 0.05
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: extracellular, including cell wall
	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for AFI273 is exc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFI273Z
3' end seq. 
>AFI273Z.Seq
----------AGCTCGTTACAGTAAATCACCATACATTTATCCATTATATGGTTTAGGTG
AATTACCACAAGCATTTGCCCGTTTATCTGCTATCTATGGTGGTACATATATGCTTAATA
AACCAATTGAAAATATTGTTTTCGATGAAGAAAAGAAAATTATTAAAGTTACATCTGAAG
GTGAAACTGTAACATGTGATAAAATTGTTTGTGATCCATCATATTTCCCAAATAAAGTTC
AAAAGAAAGGTAAAATTATTCGTTCAATTTGTATTTTGAATGGTCCAATTCCATCTGCTG
ATAAGAAATTTGAATCACTCCAAATCATTATCCCACAAAAACAAGTTAAACGTCAATCAG
ATATGTACGTTGGTGTTATGTCATCCGCTCATTGTGTCTGTCCAAAGGGTAAATACATTG
CCATCGTTTCAGCCACTGTTGAAACCAATGAACCAGAGAAAGAATTAGCACCAGCTTACG
CTTTATTAGGTCCAATTCAAGAGAAATTTGTCTATATTTCTGATTTCATTGAACCAATTG
CTGATGGTACTGCCGACAATGTTTTCATCTCAAAATCATATGATGCTACCTCTCACTTTG
AAACCACTTGTGAAGATGTCA
Length of 3' end seq. 611
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -