>SFF574 (SFF574Q) /CSM/SF/SFF5-D/SFF574Q.Seq.d/
ATTCAATTTTATTTTTAATCATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAAA
ATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATTC
CTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAAGAATGTCAT
AAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGTGAACAAAAATGGGCT
TGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATAGAAATTCATCC
AATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAATT
ACAACTCCAACCGAGTATTCTTGTTCTGATGCCAAGATTAAAGAATGNGCCTTATTAGGT
AAATCATGTAGTTTCCAAAAGAACTNTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGAA
TCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAAXXXXXXXXXX

iqfyf*sfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEECH
KSSECIVINYTPCXGEQKWACSKGTFDTCTYENSCYRNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAKIKEXALLGKSCSFQKNXCSNPTSCCPGESICEGLSSGSS---

Frame A:
iqfyf*sfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEECH
KSSECIVINYTPCXGEQKWACSKGTFDTCTYENSCYRNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAKIKEXALLGKSCSFQKNXCSNPTSCCPGESICEGLSSGSS---


Frame B:
fnfifnhsn*kknnn*ls*k*knnkgccf*kileyfl*yslyqnpmrrivikllmkknvi
nlrnvll*iihhvxvnknglalkvhlilvhmkivvieihpitkllkyqinasilmdllkl
qlqpsilvlmprlknxpy*vnhvvskrtxvqiqlpvaqvnqfvkv*alvv---


Frame C:
silfliiqikkktitn*vknkkiikdvvskkywsifydipcikilcdgl**ny**rrms*
ifgmycyklytmlx*tkmgll*ryi*ylyi*k*ll*kfiq*psc*sik*mlqs*wiy*ny
nsnrvflf*cqd*rmxlir*im*fpkellfksnfllpr*inl*rfklw*f---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLG501 (SLG501Q) /CSM/SL/SLG5-A/SLG501Q.Seq.d/                        813   0.0
SFL250 (SFL250Q) /CSM/SF/SFL2-C/SFL250Q.Seq.d/                        813   0.0
SFK426 (SFK426Q) /CSM/SF/SFK4-B/SFK426Q.Seq.d/                        813   0.0
SFK212 (SFK212Q) /CSM/SF/SFK2-A/SFK212Q.Seq.d/                        813   0.0
SFJ633 (SFJ633Q) /CSM/SF/SFJ6-B/SFJ633Q.Seq.d/                        813   0.0
SFJ257 (SFJ257Q) /CSM/SF/SFJ2-C/SFJ257Q.Seq.d/                        813   0.0
SFI696 (SFI696Q) /CSM/SF/SFI6-D/SFI696Q.Seq.d/                        813   0.0
SFI216 (SFI216Q) /CSM/SF/SFI2-A/SFI216Q.Seq.d/                        813   0.0
SFH201 (SFH201Q) /CSM/SF/SFH2-A/SFH201Q.Seq.d/                        813   0.0
SFH185 (SFH185Q) /CSM/SF/SFH1-D/SFH185Q.Seq.d/                        813   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.   666   0.0    4
AC117267|AC117267.1 Dictyostelium discoideum chromosome 2 map 4767688-4790343 strain AX4, *** SEQUENCING IN PROGRESS ***, in ordered pieces.   666   0.0    5
AC115610|AC115610.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.   666   0.0    5
AX348726|AX348726.1 Sequence 184 from Patent WO0202807.            32   0.008  5
AX344319|AX344319.1 Sequence 166 from Patent WO0200926.            32   0.008  5
AX251923|AX251923.1 Sequence 184 from Patent WO0168911.            32   0.008  5
AE014823|AE014823.1 Plasmodium falciparum 3D7 chromosome 14 section 8 of 13 of the complete sequence.    34   0.073  11
BX572090|BX572090.1 Prochlorococcus marinus MED4 complete genome; segment 1/5.    34   0.14   10
BX649641|BX649641.7 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-223O1.    32   0.32   8
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.           32   0.32   15

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   234   7e-61
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    99   5e-20
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        44   0.002
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    40   0.026
(Q99088) RecName: Full=Low-density lipoprotein receptor 2;      ...    34   2.5
QRXLL2(B40388)LDL receptor 2 precursor - African clawed frog           34   2.5
(Q99087) RecName: Full=Low-density lipoprotein receptor 1;      ...    33   3.2
BC170345_1(BC170345|pid:none) Xenopus laevis low density lipopro...    33   3.2
QRXLL1(A40388)LDL receptor 1 precursor - African clawed frog           33   3.2
AM494971_153(AM494971|pid:none) Leishmania braziliensis chromoso...    33   4.2

 psg: 1.02  gvh: 0.68  alm: 0.51  top: 0.20  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear

>> prediction for SFF574 is exc

>SFF574F.Seq
ATTCAATTTTATTTTTAATCATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAAA
ATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATTC
CTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAAGAATGTCAT
AAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGTGAACAAAAATGGGCT
TGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATAGAAATTCATCC
AATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAATT
ACAACTCCAACCGAGTATTCTTGTTCTGATGCCAAGATTAAAGAATGNGCCTTATTAGGT
AAATCATGTAGTTTCCAAAAGAACTNTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGAA
TCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAA----------