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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI256
Library AF
(Link to library)
Clone ID AFI256
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14979-1
Original site URL
Representative seq. ID AFI256P
(Link to Original site)
Representative DNA sequence 
>AFI256 (AFI256Q) /CSM/AF/AFI2-C/AFI256Q.Seq.d/
ACTTCACTCATTCATAAATAACCATGTCATTAGAGTTGAATACAATTAAAGTCAATCCAA
CTGGCTGGGGTCCAGTCGGAAAATTAGAGAAATTCACAGATATTCCATATGCACCATTTA
GCAAAGGTGATAAAATTGGTAAATGTTCAGATTGGAACTCTAATGTTAGAAATTATCAAC
GTCAAAATTATGGATCAAATGCATTCAATCCATTCACATTTAAATTGGAAGATGATGAAG
ATTCATTCACATTAGTTGATTACACTAGAGTACAAAATAAACTCAAGAATAAAGGTAAAA
CCTATCAAAAACAATTTTACCAACAAAACAAAAGAGGTGGTTCAAATGCAGGTGGTCGTG
GTGGTCGTGGTGGTATGCGTGGTGGTAGATTCGGTTCAAACAATAAATATTGGAATGATA
GACGTCAAAGAAATAGAGAATCAAGTATTGAAATCGGTTCAAGTTGGGAATCAAAAGAAG
AATTTGATTTATCAACATTTAAACAATACACTGTTXXXXXXXXXXAACCACCAAAATTCA
TCTCTATCAAAGCTATTAACGAACATGATAGTAATAGATTCGGTATTGAATTTAGAAAGA
AATTAGATTCTCAAAGAGCTGCTATCTTGGCTACTGAAATTAAAAACAATTCANCTAAAT
TTGCTAAATGGTCTATTCAATCAACTTTAGCAGGTTGTGAAATGTTAAATTTAGGTTTTG
TTTCTCGTGATTCAATTCGTGATAATAATAATCACGTCATTTTAGGTACTCAATTCTATC
CAGTTGCAGATCTCAATAAACAAAATGGTGTTGACATGAAGAATTGTTGGGGTATTTTGA
AACATATCGCTCAAACTTGTATGAAATTAGCCAATGGTAAATATCTTTTACATCGTGATC
CAAATCGTAATGTTATCAATCTTTACTCTGTTCCAGAGAATGCTTTCGATCNAATCGAAG
AAGAAACTCAAGAAGAGGAAGAAGAAGNNCAAAGCAAAGGTTGGGTTGAAGAATCAAGAG
AATAAACTCACTACNACTACTAACCGACACCCTATTCCCCTATCTCCCCAATTCAAATAT
TTATTTNAAATATTTTTAGAATAAAATAAGAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
fths*ITMSLELNTIKVNPTGWGPVGKLEKFTDIPYAPFSKGDKIGKCSDWNSNVRNYQR
QNYGSNAFNPFTFKLEDDEDSFTLVDYTRVQNKLKNKGKTYQKQFYQQNKRGGSNAGGRG
GRGGMRGGRFGSNNKYWNDRRQRNRESSIEIGSSWESKEEFDLSTFKQYTV---

---PPKFISIKAINEHDSNRFGIEFRKKLDSQRAAILATEIKNNSXKFAKWSIQSTLAGC
EMLNLGFVSRDSIRDNNNHVILGTQFYPVADLNKQNGVDMKNCWGILKHIAQTCMKLANG
KYLLHRDPNRNVINLYSVPENAFDXIEEETQEEEEEXQSKGWVEESRE*thyxy*ptpys
pispiqifixnifrik*d
Translated Amino Acid sequence (All Frames) 
Frame A:
tslihk*pch*s*iqlksiqlagvqsen*rnsqifhmhhlakviklvnvqigtlmleiin
vkimdqmhsihshlnwkmmkihsh*litleykinsrikvkpiknnftnktkevvqmqvvv
vvvvvcvvvdsvqtinigmidvkeienqvlksvqvgnqkknliyqhlnntl---

---nhqnsslsklltnmividsvlnlern*ilkellswllklktixlnllnglfnql*qv
vkc*i*vlflviqfviiiitsf*vlnsiqlqisinkmvlt*rivgvf*nislklv*n*pm
vnifyiviqivmlsiftlfqrmlsixskkklkkrkkkxkakvglknqenklttttnrhpi
plspqfkylfxifle*nk

Frame B:
lhsfinnhvirveyn*sqsnwlgssrkireihrysicti*qr**nw*mfrlel*c*klst
sklwikciqsihi*igr**rfihis*lh*stk*tqe*r*nlsktilptkqkrwfkcrwsw
wswwyaww*irfkq*ile**tskk*riky*nrfklgikrri*fini*tihc---

---ttkihlyqsy*rt****iry*i*keirfskscylgy*n*kqfx*ic*mvysinfsrl
*nvkfrfcfs*fns****srhfrysilsscrsq*tkwc*heellgyfetyrsnlyeisqw
*isfts*sks*cyqsllcsrecfrsnrrrnsrrgrrxxkqrlg*rikrinslxlltdtlf
pylpnsniyxkyf*nkir

Frame C:
fths*ITMSLELNTIKVNPTGWGPVGKLEKFTDIPYAPFSKGDKIGKCSDWNSNVRNYQR
QNYGSNAFNPFTFKLEDDEDSFTLVDYTRVQNKLKNKGKTYQKQFYQQNKRGGSNAGGRG
GRGGMRGGRFGSNNKYWNDRRQRNRESSIEIGSSWESKEEFDLSTFKQYTV---

---PPKFISIKAINEHDSNRFGIEFRKKLDSQRAAILATEIKNNSXKFAKWSIQSTLAGC
EMLNLGFVSRDSIRDNNNHVILGTQFYPVADLNKQNGVDMKNCWGILKHIAQTCMKLANG
KYLLHRDPNRNVINLYSVPENAFDXIEEETQEEEEEXQSKGWVEESRE*thyxy*ptpys
pispiqifixnifrik*d
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI256 (AFI256Q) /CSM/AF/AFI2-C/AFI256Q.Seq.d/                       2109   0.0
SLG776 (SLG776Q) /CSM/SL/SLG7-D/SLG776Q.Seq.d/                       1065   0.0
AFF124 (AFF124Q) /CSM/AF/AFF1-A/AFF124Q.Seq.d/                       1061   0.0
VFG759 (VFG759Q) /CSM/VF/VFG7-C/VFG759Q.Seq.d/                       1059   0.0
AFH506 (AFH506Q) /CSM/AF/AFH5-A/AFH506Q.Seq.d/                       1057   0.0
SFE416 (SFE416Q) /CSM/SF/SFE4-A/SFE416Q.Seq.d/                       1055   0.0
FC-366 (FC-366Q) /CSM/CG/CG-FC/FC-366Q.Seq.d/                        1049   0.0
AFN815 (AFN815Q) /CSM/AF/AFN8-A/AFN815Q.Seq.d/                       1045   0.0
AHH581 (AHH581Q) /CSM/AH/AHH5-D/AHH581Q.Seq.d/                       1037   0.0
SFK423 (SFK423Q) /CSM/SF/SFK4-A/SFK423Q.Seq.d/                       1021   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   737   0.0    17
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    38   3e-06  11
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    38   1e-05  15
AC008082|AC008082.12 Homo sapiens 3 BAC RP11-73N1 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    40   3e-05  11
AE003486|AE003486.2 Drosophila melanogaster chromosome X section 38 of 74 of the complete sequence.    48   0.002  9
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    36   0.002  17
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   0.003  16
AC034141|AC034141.4 Homo sapiens chromosome 6 clone RP11-111J1, WORKING DRAFT SEQUENCE, 26 unordered pieces.    46   0.004  8
AC068996|AC068996.11 Homo sapiens chromosome 3 clone RP11-385C5, WORKING DRAFT SEQUENCE, 7 unordered pieces.    36   0.006  10
BX294661|BX294661.14 Zebrafish DNA sequence from clone CH211-199I18 in linkage group 20.    50   0.007  10
dna update 2004. 6.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(B4K892) RecName: Full=Eukaryotic translation initiation factor ...   149   2e-34
(B4JTN0) RecName: Full=Eukaryotic translation initiation factor ...   149   2e-34
(B3LY71) RecName: Full=Eukaryotic translation initiation factor ...   149   2e-34
(B5DY99) RecName: Full=Eukaryotic translation initiation factor ...   148   3e-34
(Q16UF8) RecName: Full=Eukaryotic translation initiation factor ...   148   4e-34
(Q7QBW3) RecName: Full=Eukaryotic translation initiation factor ...   148   4e-34
AY119563_1(AY119563|pid:none) Drosophila melanogaster GM13889 fu...   147   5e-34
(B4HFV9) RecName: Full=Eukaryotic translation initiation factor ...   147   5e-34
(B4R222) RecName: Full=Eukaryotic translation initiation factor ...   147   5e-34
(Q9VCK0) RecName: Full=Eukaryotic translation initiation factor ...   147   5e-34
protein update 2009. 4.22
PSORT 

 psg: 0.84  gvh: 0.28  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFI256 is nuc
5' end seq. ID AFI256F
5' end seq. 
>AFI256F.Seq
ACTTCACTCATTCATAAATAACCATGTCATTAGAGTTGAATACAATTAAAGTCAATCCAA
CTGGCTGGGGTCCAGTCGGAAAATTAGAGAAATTCACAGATATTCCATATGCACCATTTA
GCAAAGGTGATAAAATTGGTAAATGTTCAGATTGGAACTCTAATGTTAGAAATTATCAAC
GTCAAAATTATGGATCAAATGCATTCAATCCATTCACATTTAAATTGGAAGATGATGAAG
ATTCATTCACATTAGTTGATTACACTAGAGTACAAAATAAACTCAAGAATAAAGGTAAAA
CCTATCAAAAACAATTTTACCAACAAAACAAAAGAGGTGGTTCAAATGCAGGTGGTCGTG
GTGGTCGTGGTGGTATGCGTGGTGGTAGATTCGGTTCAAACAATAAATATTGGAATGATA
GACGTCAAAGAAATAGAGAATCAAGTATTGAAATCGGTTCAAGTTGGGAATCAAAAGAAG
AATTTGATTTATCAACATTTAAACAATACACTGTT----------
Length of 5' end seq. 515
3' end seq. ID AFI256Z
3' end seq. 
>AFI256Z.Seq
----------AACCACCAAAATTCATCTCTATCAAAGCTATTAACGAACATGATAGTAAT
AGATTCGGTATTGAATTTAGAAAGAAATTAGATTCTCAAAGAGCTGCTATCTTGGCTACT
GAAATTAAAAACAATTCANCTAAATTTGCTAAATGGTCTATTCAATCAACTTTAGCAGGT
TGTGAAATGTTAAATTTAGGTTTTGTTTCTCGTGATTCAATTCGTGATAATAATAATCAC
GTCATTTTAGGTACTCAATTCTATCCAGTTGCAGATCTCAATAAACAAAATGGTGTTGAC
ATGAAGAATTGTTGGGGTATTTTGAAACATATCGCTCAAACTTGTATGAAATTAGCCAAT
GGTAAATATCTTTTACATCGTGATCCAAATCGTAATGTTATCAATCTTTACTCTGTTCCA
GAGAATGCTTTCGATCNAATCGAAGAAGAAACTCAAGAAGAGGAAGAAGAAGNNCAAAGC
AAAGGTTGGGTTGAAGAATCAAGAGAATAAACTCACTACNACTACTAACCGACACCCTAT
TCCCCTATCTCCCCAATTCAAATATTTATTTNAAATATTTTTAGAATAAAATAAGAT
Length of 3' end seq. 587
Connected seq. ID AFI256P
Connected seq. 
>AFI256P.Seq
ACTTCACTCATTCATAAATAACCATGTCATTAGAGTTGAATACAATTAAAGTCAATCCAA
CTGGCTGGGGTCCAGTCGGAAAATTAGAGAAATTCACAGATATTCCATATGCACCATTTA
GCAAAGGTGATAAAATTGGTAAATGTTCAGATTGGAACTCTAATGTTAGAAATTATCAAC
GTCAAAATTATGGATCAAATGCATTCAATCCATTCACATTTAAATTGGAAGATGATGAAG
ATTCATTCACATTAGTTGATTACACTAGAGTACAAAATAAACTCAAGAATAAAGGTAAAA
CCTATCAAAAACAATTTTACCAACAAAACAAAAGAGGTGGTTCAAATGCAGGTGGTCGTG
GTGGTCGTGGTGGTATGCGTGGTGGTAGATTCGGTTCAAACAATAAATATTGGAATGATA
GACGTCAAAGAAATAGAGAATCAAGTATTGAAATCGGTTCAAGTTGGGAATCAAAAGAAG
AATTTGATTTATCAACATTTAAACAATACACTGTT----------AACCACCAAAATTCA
TCTCTATCAAAGCTATTAACGAACATGATAGTAATAGATTCGGTATTGAATTTAGAAAGA
AATTAGATTCTCAAAGAGCTGCTATCTTGGCTACTGAAATTAAAAACAATTCANCTAAAT
TTGCTAAATGGTCTATTCAATCAACTTTAGCAGGTTGTGAAATGTTAAATTTAGGTTTTG
TTTCTCGTGATTCAATTCGTGATAATAATAATCACGTCATTTTAGGTACTCAATTCTATC
CAGTTGCAGATCTCAATAAACAAAATGGTGTTGACATGAAGAATTGTTGGGGTATTTTGA
AACATATCGCTCAAACTTGTATGAAATTAGCCAATGGTAAATATCTTTTACATCGTGATC
CAAATCGTAATGTTATCAATCTTTACTCTGTTCCAGAGAATGCTTTCGATCNAATCGAAG
AAGAAACTCAAGAAGAGGAAGAAGAAGNNCAAAGCAAAGGTTGGGTTGAAGAATCAAGAG
AATAAACTCACTACNACTACTAACCGACACCCTATTCCCCTATCTCCCCAATTCAAATAT
TTATTTNAAATATTTTTAGAATAAAATAAGAT
Length of connected seq. 1102
Full length Seq ID -
Full length Seq. -
Length of full length seq. -