>SFF439 (SFF439Q) /CSM/SF/SFF4-B/SFF439Q.Seq.d/
ATATCGTTATCGATTTTAATGATGTTAAAAACCAATACTGGTGGAAATACATCAATTATA
AAGAATAGAAAGGATTCAGATATTAGTGATAGTAGTGGTGATGAAGATTATAGATCAAGT
TCAAAATCACTTCAAAAACTTTTTATTGGTTTATCAGAAGATTTCACATGCTCAATTTGT
TATGAATTATTTGAAAGACCAATTATATTACCATGTAATCATAATTTTTGTAAGTCATGT
ATTGAGGATATGGTAATTGGACAAAAACAAATATTTCATTGTCCATTTTGTAGAACAGAG
GTAAAGTTAACTGAGAAAGGTGTTGATGGATTACCAGTAAATTCATTTTTATTTACAGCA
GTGGAAAAAATGAAAAACGCCAATGCATTTTGTCCAAAGTTTTGTGATAATTGTACATCT
AAAAAGGTGACAAGAAAATGTGAGACCTGTTCGATATCCTTATGTAAGGCATGTGAGAGT
AGCCATGTCGCAAAGAATGGGGGTCACAAAACCATCTATTCCGATAGTCACATTGCAAGT
GATTTCGATATTGAGACTCATATTAACAAGGATAGCGATATCGATCCACCAACCTATTTA
CCATTCACCCATCTCXXXXXXXXXX

ISLSILMMLKTNTGGNTSIIKNRKDSDISDSSGDEDYRSSSKSLQKLFIGLSEDFTCSIC
YELFERPIILPCNHNFCKSCIEDMVIGQKQIFHCPFCRTEVKLTEKGVDGLPVNSFLFTA
VEKMKNANAFCPKFCDNCTSKKVTRKCETCSISLCKACESSHVAKNGGHKTIYSDSHIAS
DFDIETHINKDSDIDPPTYLPFTH---

Frame A:
ISLSILMMLKTNTGGNTSIIKNRKDSDISDSSGDEDYRSSSKSLQKLFIGLSEDFTCSIC
YELFERPIILPCNHNFCKSCIEDMVIGQKQIFHCPFCRTEVKLTEKGVDGLPVNSFLFTA
VEKMKNANAFCPKFCDNCTSKKVTRKCETCSISLCKACESSHVAKNGGHKTIYSDSHIAS
DFDIETHINKDSDIDPPTYLPFTH---


Frame B:
yryrf**c*kpilveihql*rieriqilvivvvmkiidqvqnhfknfllvyqkishaqfv
mnylkdqlyyhviiifvshvlriw*ldknkyfivhfveqr*s*lrkvlmdyq*ihfylqq
wkk*ktpmhfvqsfviivhlkr*qenvrpvrypyvrhvrvamsqrmgvtkpsipivtlqv
isilrliltriaisihqpiyhspi---


Frame C:
ividfndvknqywwkyinyke*kgfry****w**rl*ikfkitsktfywfirrfhmlnll
*ii*ktnyitm*s*fl*vmy*gygnwtktnislsil*nrgkvn*erc*witskfifiyss
gknekrqcilskvl**lyi*kgdkkm*dlfdilm*gm*e*pcrkewgsqnhlfr*shck*
fry*dsy*qg*ryrstnlftihps---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFF439 (SFF439Q) /CSM/SF/SFF4-B/SFF439Q.Seq.d/                        722   0.0
SFD403 (SFD403Q) /CSM/SF/SFD4-A/SFD403Q.Seq.d/                        718   0.0
AFO616 (AFO616Q) /CSM/AF/AFO6-A/AFO616Q.Seq.d/                        460   e-128
SFI291 (SFI291Q) /CSM/SF/SFI2-D/SFI291Q.Seq.d/                         70   4e-11
VSE532 (VSE532Q) /CSM/VS/VSE5-B/VSE532Q.Seq.d/                         38   0.16
VFI490 (VFI490Q) /CSM/VF/VFI4-D/VFI490Q.Seq.d/                         38   0.16
SHI859 (SHI859Q) /CSM/SH/SHI8-C/SHI859Q.Seq.d/                         38   0.16
VHQ475 (VHQ475Q) /CSM/VH/VHQ4-D/VHQ475Q.Seq.d/                         36   0.62
VHJ842 (VHJ842Q) /CSM/VH/VHJ8-B/VHJ842Q.Seq.d/                         36   0.62
VHH612 (VHH612Q) /CSM/VH/VHH6-A/VHH612Q.Seq.d/                         36   0.62

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    38   0.001  11
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           32   0.048  16
AE014823|AE014823.1 Plasmodium falciparum 3D7 chromosome 14 section 8 of 13 of the complete sequence.    30   0.061  10
AC090052|AC090052.15 Homo sapiens 12 BAC RP11-295G12 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    32   0.14   8
BZ281154|BZ281154.1 CH230-429L16.TV CHORI-230 Segment 2 Rattus norvegicus genomic clone CH230-429L16, DNA sequence.    32   0.20   3
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    30   0.22   9
BX248586|BX248586.1 Blochmannia floridanus complete genome; segment 3/3.    38   0.47   7
AC004153|AC004153.7 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    32   0.50   10
AC022988|AC022988.2 Homo sapiens chromosome 4 clone RP11-637O11 map 4, WORKING DRAFT SEQUENCE, 17 unordered pieces.    38   0.54   8
AC096904|AC096904.7 Rattus norvegicus clone CH230-154M3, *** SEQUENCING IN PROGRESS ***, 12 unordered pieces.    46   0.57   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK297205_1(AK297205|pid:none) Homo sapiens cDNA FLJ57031 complet...    84   2e-15
AF026565_1(AF026565|pid:none) Mus musculus ring finger protein (...    80   5e-14
(P82458) RecName: Full=Midline-1; AltName: Full=Tripartite motif...    80   5e-14
AB384764_1(AB384764|pid:none) Synthetic construct DNA, clone: pF...    80   6e-14
(O15344) RecName: Full=Midline-1;          EC=6.3.2.-; AltName: ...    80   6e-14
AK299061_1(AK299061|pid:none) Homo sapiens cDNA FLJ58683 complet...    80   6e-14
AK291215_1(AK291215|pid:none) Homo sapiens cDNA FLJ76288 complet...    80   6e-14
BC076998_1(BC076998|pid:none) Xenopus tropicalis midline 1 (Opit...    79   8e-14
(P82457) RecName: Full=Midline-1; AltName: Full=Tripartite motif...    78   2e-13
BC053704_1(BC053704|pid:none) Mus musculus midline 1, mRNA (cDNA...    77   3e-13

 psg: 0.82  gvh: 0.50  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 20.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for SFF439 is nuc

>SFF439F.Seq
ATATCGTTATCGATTTTAATGATGTTAAAAACCAATACTGGTGGAAATACATCAATTATA
AAGAATAGAAAGGATTCAGATATTAGTGATAGTAGTGGTGATGAAGATTATAGATCAAGT
TCAAAATCACTTCAAAAACTTTTTATTGGTTTATCAGAAGATTTCACATGCTCAATTTGT
TATGAATTATTTGAAAGACCAATTATATTACCATGTAATCATAATTTTTGTAAGTCATGT
ATTGAGGATATGGTAATTGGACAAAAACAAATATTTCATTGTCCATTTTGTAGAACAGAG
GTAAAGTTAACTGAGAAAGGTGTTGATGGATTACCAGTAAATTCATTTTTATTTACAGCA
GTGGAAAAAATGAAAAACGCCAATGCATTTTGTCCAAAGTTTTGTGATAATTGTACATCT
AAAAAGGTGACAAGAAAATGTGAGACCTGTTCGATATCCTTATGTAAGGCATGTGAGAGT
AGCCATGTCGCAAAGAATGGGGGTCACAAAACCATCTATTCCGATAGTCACATTGCAAGT
GATTTCGATATTGAGACTCATATTAACAAGGATAGCGATATCGATCCACCAACCTATTTA
CCATTCACCCATCTC----------