>SFF405 (SFF405Q) /CSM/SF/SFF4-A/SFF405Q.Seq.d/
XXXXXXXXXXACGTGGTGTAAATAAATTCTTGTCTCCCATTGGGATGTTTGTTAATGTCA
TTCAAATGTAAACAAACTGATGGTAATAAATTACCATTCCTTCAATATTTGGCTGGTATG
GCAATGGTCGAAGCAATTCACTCTTTCCCAAGTGCATCTGATTTAAATCTTCGTTTGAAG
TGGCCAAACGATATCCTATAGTAATGAACCAGGTATGAAAGTTGGTGGTGTCCTCTGTGA
ATCAAATTATCTTAACAATGAATTTGATGTTGTCATTGGTGTTGGTGTTAATCTTTCAAA
TTCAAATAATCCATCAACAACTATAAATCAATTAATTCAAAAGAAAAAATATGGATTTTC
ATCATCATCATTGGCAACAAGTACTGATATTCCAATTTATATTAGTAGAGAAGAATTGGT
TTCAAAATTCTTTAACAAATTCGAACCAATGTTTATGGAATTTACAAGAGATGGCTTTAA
TGCAGATCTTGAAAATAGATACACTGACTTATGGATGCATACAAATCAAATTGTAAAATT
TAAAGAAAGAGATAACCATCATGTTAAAATCATTGGTATTTCTGATAGTGGTTTCCTTAA
AGCTATTGAATGTGATGCTGATGGTAATACATCTTCTGACTCTGAAACCGTTGAGCTTCA
CCCAGATNGTACTTCATTNATATTCAAAACTAATTTAANCAAAAATCACAACAACCAAAA

---wck*ilvshwdvc*chsnvnklmvinyhsfniwlvwqwskqftlsqvhli*ifv*SG
QTISYSNEPGMKVGGVLCESNYLNNEFDVVIGVGVNLSNSNNPSTTINQLIQKKKYGFSS
SSLATSTDIPIYISREELVSKFFNKFEPMFMEFTRDGFNADLENRYTDLWMHTNQIVKFK
ERDNHHVKIIGISDSGFLKAIECDADGNTSSDSETVELHPDXTSXIFKTNLXKNHNNQ

Frame A:
---rgvnkflspigmfvnviqm*tn*w**itipsifgwygngrsnslfpkci*fkssfev
akrypivmnqv*klvvssvnqiiltmnlmlslvlvlifqiqiihqql*in*fkrknmdfh
hhhwqqvlifqfilveknwfqnsltnsnqclwnlqemalmqilkidtltygciqikl*nl
kkeitimlkslvflivvslkllnvmlmvihlltlkplsftqxvlhxyskli*xkitttk


Frame B:
---vv*insclplgcllmsfkckqtdgnklpflqylagmamveaihsfpsasdlnlrlkw
pndil***tryeswwcpl*ikls*q*i*cchwcwc*sfkfk*sinnyksinskekiwifi
iiignky*ysnly**rrigfkil*qirtnvygiykrwl*crs*k*ih*lmdayksncki*
rkr*psc*nhwyf**wfp*sy*m*c*w*yif*l*nr*asprxyfixiqn*fxqksqqpk


Frame C:
---wck*ilvshwdvc*chsnvnklmvinyhsfniwlvwqwskqftlsqvhli*ifv*SG
QTISYSNEPGMKVGGVLCESNYLNNEFDVVIGVGVNLSNSNNPSTTINQLIQKKKYGFSS
SSLATSTDIPIYISREELVSKFFNKFEPMFMEFTRDGFNADLENRYTDLWMHTNQIVKFK
ERDNHHVKIIGISDSGFLKAIECDADGNTSSDSETVELHPDXTSXIFKTNLXKNHNNQ

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFF405 (SFF405Q) /CSM/SF/SFF4-A/SFF405Q.Seq.d/                       1390   0.0
SFD294 (SFD294Q) /CSM/SF/SFD2-D/SFD294Q.Seq.d/                       1316   0.0
SFJ133 (SFJ133Q) /CSM/SF/SFJ1-B/SFJ133Q.Seq.d/                       1279   0.0
CHB590 (CHB590Q) /CSM/CH/CHB5-D/CHB590Q.Seq.d/                       1233   0.0
AFC713 (AFC713Q) /CSM/AF/AFC7-A/AFC713Q.Seq.d/                       1191   0.0
SFE209 (SFE209Q) /CSM/SF/SFE2-A/SFE209Q.Seq.d/                       1065   0.0
AFN413 (AFN413Q) /CSM/AF/AFN4-A/AFN413Q.Seq.d/                        894   0.0
AFO680 (AFO680Q) /CSM/AF/AFO6-D/AFO680Q.Seq.d/                        878   0.0
AFH686 (AFH686Q) /CSM/AF/AFH6-D/AFH686Q.Seq.d/                        819   0.0
SFI426 (SFI426Q) /CSM/SF/SFI4-B/SFI426Q.Seq.d/                        799   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   5e-07  18
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           36   1e-06  23
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    36   9e-06  17
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    34   1e-04  17
AC116920|AC116920.2 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    42   2e-04  9
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    34   3e-04  16
BX547932|BX547932.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-269E10.    40   0.002  6
BX511250|BX511250.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-57K11.    34   0.004  10
AR271569|AR271569.1 Sequence 1 from patent US 6503729.             32   0.005  25
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            34   0.007  20

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AP008208_425(AP008208|pid:none) Oryza sativa (japonica cultivar-...    89   2e-26
AP005804_18(AP005804|pid:none) Oryza sativa Japonica Group genom...    86   1e-25
AC006053_17(AC006053|pid:none) Arabidopsis thaliana chromosome 2...    87   4e-24
G84651(G84651)biotin holocarboxylase synthetase [imported] - Ara...    85   2e-23
AF414940_1(AF414940|pid:none) Arabidopsis thaliana holocarboxyla...    84   2e-23
CP000582_324(CP000582|pid:none) Ostreococcus lucimarinus CCE9901...    85   3e-23
(P50747) RecName: Full=Biotin--protein ligase;          EC=6.3.4...    68   4e-22
(Q920N2) RecName: Full=Biotin--protein ligase;          EC=6.3.4...    65   1e-20
AK168925_1(AK168925|pid:none) Mus musculus 16 days embryo kidney...    65   1e-20
BC153857_1(BC153857|pid:none) Bos taurus similar to holocarboxyl...    60   9e-20

 psg: 0.75  gvh: 0.37  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for SFF405 is cyt

>SFF405Z.Seq
----------ACGTGGTGTAAATAAATTCTTGTCTCCCATTGGGATGTTTGTTAATGTCA
TTCAAATGTAAACAAACTGATGGTAATAAATTACCATTCCTTCAATATTTGGCTGGTATG
GCAATGGTCGAAGCAATTCACTCTTTCCCAAGTGCATCTGATTTAAATCTTCGTTTGAAG
TGGCCAAACGATATCCTATAGTAATGAACCAGGTATGAAAGTTGGTGGTGTCCTCTGTGA
ATCAAATTATCTTAACAATGAATTTGATGTTGTCATTGGTGTTGGTGTTAATCTTTCAAA
TTCAAATAATCCATCAACAACTATAAATCAATTAATTCAAAAGAAAAAATATGGATTTTC
ATCATCATCATTGGCAACAAGTACTGATATTCCAATTTATATTAGTAGAGAAGAATTGGT
TTCAAAATTCTTTAACAAATTCGAACCAATGTTTATGGAATTTACAAGAGATGGCTTTAA
TGCAGATCTTGAAAATAGATACACTGACTTATGGATGCATACAAATCAAATTGTAAAATT
TAAAGAAAGAGATAACCATCATGTTAAAATCATTGGTATTTCTGATAGTGGTTTCCTTAA
AGCTATTGAATGTGATGCTGATGGTAATACATCTTCTGACTCTGAAACCGTTGAGCTTCA
CCCAGATNGTACTTCATTNATATTCAAAACTAATTTAANCAAAAATCACAACAACCAAAA