>AFI252 (AFI252Q) /CSM/AF/AFI2-C/AFI252Q.Seq.d/
CAAGNAGTACAATTAAAAAAGATTCAAGNATAAAGAAGGTTCAGATTATATTAATGCTAA
TTATATTGATGGAGCATACCCAAAACAATTTATTTGTACACAAGGACCATTACCAAATAC
AATTGCTGATTTTTGGAGAATGGTATGGGAAAATCGATGTCGTATTATTGTTATGCTTTC
AAGAGAGTCTGAAAACTGTAGAATAAAATGTGATAGATATTGGCCAGAACAAATTGGAGG
TGAACAATTTTCAATATATGGTAATGGTAATGAAGTATTTGGCACATACAGTGTCGAATT
GGTTGAGGTTATCCAAGATCCAGAGAGAGAGATAATCACAAGAAACATTAGACTTACATT
TGAGGGTGAGACAAGAGATATCACTCAATATCAATATGAAGGTTGGCCAGATCATAACAT
CCCAGATCACACTCAACCATTCCGTCAGTTGNTACATAGTATCACAAATCGNNAAAATCA
AATCATACCATCATCAGATAGAAATGTTCCAATCATNGNTNATTGNNNNGCANGAGNNGG
NNNAAXXXXXXXXXX

kxyn*KRFKXKEGSDYINANYIDGAYPKQFICTQGPLPNTIADFWRMVWENRCRIIVMLS
RESENCRIKCDRYWPEQIGGEQFSIYGNGNEVFGTYSVELVEVIQDPEREIITRNIRLTF
EGETRDITQYQYEGWPDHNIPDHTQPFRQLXHSITNRXNQIIPSSDRNVPIXXXXXAXXG
X---

Frame A:
qxvqlkkiqx*rrfrly*c*ly*wsipktiylytrtitkync*flengmgksmsyycyaf
krv*kl*nkm**ilartnwr*tifniw*w**siwhiqcrig*gyprsrerdnhkkh*tyi
*g*dkryhsisi*rlars*hprshstipsvxt*yhksxksnhtiir*kcsnhxxlxxxxx
x---


Frame B:
kxyn*KRFKXKEGSDYINANYIDGAYPKQFICTQGPLPNTIADFWRMVWENRCRIIVMLS
RESENCRIKCDRYWPEQIGGEQFSIYGNGNEVFGTYSVELVEVIQDPEREIITRNIRLTF
EGETRDITQYQYEGWPDHNIPDHTQPFRQLXHSITNRXNQIIPSSDRNVPIXXXXXAXXG
X---


Frame C:
xstikkdsxikkvqiilmliilmehtqnnlfvhkdhyqiqllifgewygkidvvlllcfq
eslktve*nvidigqnklevnnfqymvmvmkylahtvsnwlrlskiqrer*sqetldlhl
rvrqeislninmkvgqiitsqitlnhsvsxyivsqixkiksyhhqiemfqsxxixxxexx
---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI252 (AFI252Q) /CSM/AF/AFI2-C/AFI252Q.Seq.d/                        993   0.0
SSC679 (SSC679Q) /CSM/SS/SSC6-D/SSC679Q.Seq.d/                        979   0.0
AFM223 (AFM223Q) /CSM/AF/AFM2-A/AFM223Q.Seq.d/                        521   e-147
SSE467 (SSE467Q) /CSM/SS/SSE4-C/SSE467Q.Seq.d/                        159   5e-38
AFN131 (AFN131Q) /CSM/AF/AFN1-B/AFN131Q.Seq.d/                         58   1e-07
AFC372 (AFC372Q) /CSM/AF/AFC3-C/AFC372Q.Seq.d/                         58   1e-07
SFC862 (SFC862Q) /CSM/SF/SFC8-C/SFC862Q.Seq.d/                         42   0.009
VFN603 (VFN603Q) /CSM/VF/VFN6-A/VFN603Q.Seq.d/                         38   0.14
VSH673 (VSH673Q) /CSM/VS/VSH6-D/VSH673Q.Seq.d/                         36   0.54
VFO819 (VFO819Q) /CSM/VF/VFO8-A/VFO819Q.Seq.d/                         36   0.54

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L07125|L07125.1 Dictyostelium discoideum phosphotyrosine phosphatase (PTP1) gene, complete cds.   866   0.0    3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   866   0.0    8
L15420|L15420.1 Dictyostelium discoideum protein tyrosine phosphatase mRNA, complete cds.    58   1e-08  2
BI741968|BI741968.1 kt83e12.y1 Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to TR:Q92124 Q92124 PHOSPHOTYROSYL-PROTEIN PHOSPHATASE ;, mRNA sequence.    38   3e-08  4
BI509395|BI509395.1 BB170015B20B09 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170015B20B09 5', mRNA sequence.    48   3e-04  2
AF198450|AF198450.1 Xenopus laevis receptor protein tyrosine phosphatase CRYP-alpha mRNA, partial cds.    44   4e-04  2
BW109613|BW109613.1 Ciona intestinalis cDNA, clone:rcicl072k12, 3' end, single read.    54   0.002  1
AW671706|AW671706.1 LG1_349_A06.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA sequence.    42   0.004  2
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    40   0.009  7
CD202354|CD202354.1 MS1-0131U-A356-D10-U.B MS1-0131 Schistosoma mansoni cDNA clone MS1-0131U-A356-D10.B, mRNA sequence.    52   0.009  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L07125_1(L07125|pid:none) Dictyostelium discoideum phosphotyrosi...   344   7e-94
(P34137) RecName: Full=Tyrosine-protein phosphatase 1;          ...   344   7e-94
A44267(A44267)protein-tyrosine-phosphatase (EC 3.1.3.48), nonrec...   344   7e-94
Z46811_2(Z46811|pid:none) Caenorhabditis elegans Cosmid C09D8, c...   127   2e-28
Z46811_4(Z46811|pid:none) Caenorhabditis elegans Cosmid C09D8, c...   127   2e-28
(Q9BMN8) RecName: Full=Tyrosine-protein phosphatase Lar-like;   ...   127   2e-28
I50212(I50212) protein-tyrosine-phosphatase (EC 3.1.3.48) - chic...   127   2e-28
AJ311886_1(AJ311886|pid:none) Danio rerio partial mRNA for recep...   126   4e-28
CU539059_1(CU539059|pid:none) Zebrafish DNA sequence from clone ...   126   4e-28
BC104812_1(BC104812|pid:none) Homo sapiens protein tyrosine phos...   125   7e-28

 psg: 0.63  gvh: 0.47  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFI252 is nuc

>AFI252F.Seq
CAAGNAGTACAATTAAAAAAGATTCAAGNATAAAGAAGGTTCAGATTATATTAATGCTAA
TTATATTGATGGAGCATACCCAAAACAATTTATTTGTACACAAGGACCATTACCAAATAC
AATTGCTGATTTTTGGAGAATGGTATGGGAAAATCGATGTCGTATTATTGTTATGCTTTC
AAGAGAGTCTGAAAACTGTAGAATAAAATGTGATAGATATTGGCCAGAACAAATTGGAGG
TGAACAATTTTCAATATATGGTAATGGTAATGAAGTATTTGGCACATACAGTGTCGAATT
GGTTGAGGTTATCCAAGATCCAGAGAGAGAGATAATCACAAGAAACATTAGACTTACATT
TGAGGGTGAGACAAGAGATATCACTCAATATCAATATGAAGGTTGGCCAGATCATAACAT
CCCAGATCACACTCAACCATTCCGTCAGTTGNTACATAGTATCACAAATCGNNAAAATCA
AATCATACCATCATCAGATAGAAATGTTCCAATCATNGNTNATTGNNNNGCANGAGNNGG
NNNAA----------