>SFF249 (SFF249Q) /CSM/SF/SFF2-C/SFF249Q.Seq.d/
XXXXXXXXXXCAGGTATTGCTCGTTCAGTTTATCGTTCTCATGGTCGTTATCTTCAAACT
TATATGACTCAATATAAAGGTCACTATTTCACTGGTGATGGTGTAAAACTTGACTCTGAT
GGTTATTATTGGATCGAAGGTAGAGTAGATGATGTTATCAATGTTAGTGGTCATAGATTA
GGTACTGCTGAATTAGAGAGTGCCCTCGTTGGTTGTTCAATTTGTGCAGAGGCTGCCGTT
GTTGGTTATCCTCATGATATCAAAGGTCAAGGTATTTTAGCATTTTGTACACTCAAAGAA
GGTTATCAAGAGGATGAATCAAATGTTATTATGATGTTAAAAAAAGAAGTTAGAAATGTA
ATTGGTCCATTTGCAACACCTGATGTAATTGTTATAACACCTTCACTTCCAAAAACTCGT
AGTGGTAAAATTATGAGAAGAATTCTTAGAAAAATTGGTTGTCATGAATCTTCTGCTGAA
CAATTAGGTGATATTTCAACTTTAGCTGAACCTGAAGTTGTCAAACTTTTAATTGAAAAA
GTTTCAAAAGTATTCCAAAAACTCATTAAAAAAATAAAAAATAAAAAAAAATAATTCAGA
TTCGAAAT

---GIARSVYRSHGRYLQTYMTQYKGHYFTGDGVKLDSDGYYWIEGRVDDVINVSGHRLG
TAELESALVGCSICAEAAVVGYPHDIKGQGILAFCTLKEGYQEDESNVIMMLKKEVRNVI
GPFATPDVIVITPSLPKTRSGKIMRRILRKIGCHESSAEQLGDISTLAEPEVVKLLIEKV
SKVFQKLIKKIKNKKK*frfe

Frame A:
---rycsfslsfswslssnlydsi*rslfhw*wckt*l*wllldrr*sr*cyqc*ws*ir
yc*irecprwlfnlcrgcrcwlss*yqrsryfsilytqrrlsrg*ikcyydvkkrs*kcn
wsicnt*cncyntftskns*w*nyekns*knwls*ifc*tir*yfnfs*t*scqtfn*ks
fksipkth*knkk*kkiiqirn


Frame B:
---GIARSVYRSHGRYLQTYMTQYKGHYFTGDGVKLDSDGYYWIEGRVDDVINVSGHRLG
TAELESALVGCSICAEAAVVGYPHDIKGQGILAFCTLKEGYQEDESNVIMMLKKEVRNVI
GPFATPDVIVITPSLPKTRSGKIMRRILRKIGCHESSAEQLGDISTLAEPEVVKLLIEKV
SKVFQKLIKKIKNKKK*frfe


Frame C:
---vllvqfivlmvvifkli*lnikvtislvmv*nltlmviigskve*mmlsmlvvid*v
lln*rvpslvvqfvqrlpllvilmiskvkvf*hfvhskkvikrmnqmll*c*kkklem*l
vhlqhlm*ll*hlhfqklvvvkl*eefleklvvmnlllnn*vifql*lnlklsnf*lkkf
qkysknslkk*kikknnsdsk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFF249 (SFF249Q) /CSM/SF/SFF2-C/SFF249Q.Seq.d/                       1063   0.0
VHJ869 (VHJ869Q) /CSM/VH/VHJ8-C/VHJ869Q.Seq.d/                       1049   0.0
SFB864 (SFB864Q) /CSM/SF/SFB8-C/SFB864Q.Seq.d/                       1049   0.0
VHD191 (VHD191Q) /CSM/VH/VHD1-D/VHD191Q.Seq.d/                       1033   0.0
CFJ158 (CFJ158Q) /CSM/CF/CFJ1-C/CFJ158Q.Seq.d/                       1033   0.0
VHO673 (VHO673Q) /CSM/VH/VHO6-D/VHO673Q.Seq.d/                       1027   0.0
SFF506 (SFF506Q) /CSM/SF/SFF5-A/SFF506Q.Seq.d/                       1027   0.0
AFE757 (AFE757Q) /CSM/AF/AFE7-C/AFE757Q.Seq.d/                       1027   0.0
CFH749 (CFH749Q) /CSM/CF/CFH7-C/CFH749Q.Seq.d/                       1023   0.0
CFI106 (CFI106Q) /CSM/CF/CFI1-A/CFI106Q.Seq.d/                       1019   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.   650   0.0    4
AX489103|AX489103.1 Sequence 6403 from Patent WO02053728.          70   6e-20  4
AZ930496|AZ930496.1 474.dhz54h09.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz54h09.s1, DNA sequence.    72   2e-14  2
CD978883|CD978883.1 QAF6c12.yg QAF Zea mays cDNA clone QAF6c12, mRNA sequence.    42   8e-14  4
AW037040|AW037040.1 614025A12.y1 614 - root cDNA library from Walbot Lab Zea mays cDNA, mRNA sequence.    42   3e-13  4
BI674812|BI674812.1 949067E10.y2 949 - Juvenile leaf and shoot cDNA from Steve Moose Zea mays cDNA, mRNA sequence.    42   5e-13  4
CA269673|CA269673.1 SCMCRT3083F09.g RT3 Saccharum officinarum cDNA clone SCMCRT3083F09 5', mRNA sequence.    42   8e-13  4
CA258874|CA258874.1 SCBGRT3013E01.g RT3 Saccharum officinarum cDNA clone SCBGRT3013E01 5', mRNA sequence.    42   1e-12  4
CF635493|CF635493.1 zmrww00_0A20-011-b10.s2 zmrww00 Zea mays cDNA, mRNA sequence.    42   1e-12  4
BI478599|BI478599.1 949067E10.x1 949 - Juvenile leaf and shoot cDNA from Steve Moose Zea mays cDNA, mRNA sequence.    42   1e-11  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115612_37(AC115612|pid:none) Dictyostelium discoideum chromoso...   363   3e-99
(Q54Z60) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   363   3e-99
CP000158_1385(CP000158|pid:none) Hyphomonas neptunium ATCC 15444...   215   9e-55
(Q6MNF1) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   211   9e-54
DQ782753_1(DQ782753|pid:none) Polytomella parva plastid acetyl C...   210   2e-53
CP000927_111(CP000927|pid:none) Caulobacter sp. K31, complete ge...   210   3e-53
CP000157_932(CP000157|pid:none) Erythrobacter litoralis HTCC2594...   209   5e-53
(A1U2S9) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   208   1e-52
CP001103_707(CP001103|pid:none) Alteromonas macleodii 'Deep ecot...   208   1e-52
CP000285_857(CP000285|pid:none) Chromohalobacter salexigens DSM ...   207   2e-52

 psg: 0.60  gvh: 0.25  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.47  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.50
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  4.0 %: peroxisomal

>> prediction for SFF249 is nuc

>SFF249Z.Seq
----------CAGGTATTGCTCGTTCAGTTTATCGTTCTCATGGTCGTTATCTTCAAACT
TATATGACTCAATATAAAGGTCACTATTTCACTGGTGATGGTGTAAAACTTGACTCTGAT
GGTTATTATTGGATCGAAGGTAGAGTAGATGATGTTATCAATGTTAGTGGTCATAGATTA
GGTACTGCTGAATTAGAGAGTGCCCTCGTTGGTTGTTCAATTTGTGCAGAGGCTGCCGTT
GTTGGTTATCCTCATGATATCAAAGGTCAAGGTATTTTAGCATTTTGTACACTCAAAGAA
GGTTATCAAGAGGATGAATCAAATGTTATTATGATGTTAAAAAAAGAAGTTAGAAATGTA
ATTGGTCCATTTGCAACACCTGATGTAATTGTTATAACACCTTCACTTCCAAAAACTCGT
AGTGGTAAAATTATGAGAAGAATTCTTAGAAAAATTGGTTGTCATGAATCTTCTGCTGAA
CAATTAGGTGATATTTCAACTTTAGCTGAACCTGAAGTTGTCAAACTTTTAATTGAAAAA
GTTTCAAAAGTATTCCAAAAACTCATTAAAAAAATAAAAAATAAAAAAAAATAATTCAGA
TTCGAAAT