>AFI239 (AFI239Q) /CSM/AF/AFI2-B/AFI239Q.Seq.d/
XXXXXXXXXXAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTA
CGATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGT
CATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGC
TCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGA
TGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTA
CGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGC
CGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTT
CAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAA
CTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAAC
TGATGATAATGTTGAATCTAAAAAAGATTCTTCTGCTAAACATGTATGANTTATAAATAA
ATTTAATAAATA

---VVPAISVDSVANKARYDFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAP
VKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDAD
SNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTD
DNVESKKDSSAKHV*xinkfnk

Frame A:
---klfqpslliqlpiklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmml
qlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmp
tqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlkl
mimlnlkkilllnmyxl*inlin


Frame B:
---scsshlc*fscq*sslrfr*krnlliryrlqmdprchww*sfsic*istrtrik*cs
s*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*cr
lk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rch*iqitiisn*
**c*i*krffc*tcmxyk*i**i


Frame C:
---VVPAISVDSVANKARYDFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAP
VKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDAD
SNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTD
DNVESKKDSSAKHV*xinkfnk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI239 (AFI239Q) /CSM/AF/AFI2-B/AFI239Q.Seq.d/                       1187   0.0
AFL635 (AFL635Q) /CSM/AF/AFL6-B/AFL635Q.Seq.d/                       1168   0.0
AFN839 (AFN839Q) /CSM/AF/AFN8-B/AFN839Q.Seq.d/                       1164   0.0
AFI355 (AFI355Q) /CSM/AF/AFI3-C/AFI355Q.Seq.d/                       1154   0.0
SLA686 (SLA686Q) /CSM/SL/SLA6-D/SLA686Q.Seq.d/                       1148   0.0
AFO829 (AFO829Q) /CSM/AF/AFO8-B/AFO829Q.Seq.d/                       1148   0.0
AFM402 (AFM402Q) /CSM/AF/AFM4-A/AFM402Q.Seq.d/                       1148   0.0
AFL565 (AFL565Q) /CSM/AF/AFL5-C/AFL565Q.Seq.d/                       1148   0.0
AFI356 (AFI356Q) /CSM/AF/AFI3-C/AFI356Q.Seq.d/                       1148   0.0
AFH647 (AFH647Q) /CSM/AF/AFH6-B/AFH647Q.Seq.d/                       1148   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   4e-08  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   4e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   4e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   4e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   167   2e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   161   1e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   156   4e-37
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   156   5e-37
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   156   5e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   1e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   1e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   147   2e-34
BC097044_1(BC097044|pid:none) Danio rerio protein disulfide isom...   147   2e-34
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   147   2e-34

 psg: 0.75  gvh: 0.38  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFI239 is nuc

>AFI239Z.Seq
----------AAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTA
CGATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGT
CATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGC
TCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGA
TGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTA
CGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGC
CGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTT
CAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAA
CTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAAC
TGATGATAATGTTGAATCTAAAAAAGATTCTTCTGCTAAACATGTATGANTTATAAATAA
ATTTAATAAATA