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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFE825
Library SF
(Link to library)
Clone ID SFE825
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16419-1
Original site URL
Representative seq. ID SFE825P
(Link to Original site)
Representative DNA sequence 
>SFE825 (SFE825Q) /CSM/SF/SFE8-B/SFE825Q.Seq.d/
ATTCAATTTTATTTTTAATNAATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAA
AATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATT
CCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAAGAATGTCA
TAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGTGAACAAAAATGGGC
TTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATAGAAATTCATC
CAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAAT
TACAACTCCAACCGAGTATTCTTGTTCTGATGCCAAGATTAAAGAATGTGCCTTATTAGG
TAAATCATGTAGTTTCCAAAAGAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGA
ATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTGX
XXXXXXXXXATGATCATGGTAAAAAATGTTGTGTAAAAGATTCATTGTGATGAAGTTTGT
GATTTAGATTGTGGTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGT
GTCCGTTCAGAAAGACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCNCCA
GGCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCA
CCACCAAAATGCTCTTTAAGATGTCCNCCAAGACATGAATGTAGAGTCAACCACTTTGGT
GAAGAATGTTGTGTTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCAT
GAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCA
AAATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAA
TGCTGTGTTAAAAGTAGAAATGATTGTTTAACTTGTGAAGACCTANACTGTGCAAAGAAA
AGGTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fnfifnxfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEECH
KSSECIVINYTPCXGEQKWACSKGTFDTCTYENSCYRNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAKIKECALLGKSCSFQKNSCSNPTSCCPGESICEGLSSGSSTSGGGSSG-
--

---DHGKKCCVKDSL**sl*frlw*rf*m*n*t*wfkmlcpfrkttpttt*km**empxr
p*m*s*stwkrmlrccp*tttkmlfkmsxkt*m*sqplw*rmlc*sspr*mfiemstrp*
m*s*stwkrmlrccp*tttkmlikmstkt*m*nqslw*rmlc*k*k*lfnl*rpxlckek
v
Translated Amino Acid sequence (All Frames) 
Frame A:
iqfyf*xiqikkktitn*vknkkiikdvvskkywsifydipcikilcdgl**ny**rrms
*ifgmycyklytmlx*tkmgll*ryi*ylyi*k*ll*kfiq*psc*sik*mlqs*wiy*n
ynsnrvflf*cqd*rmclir*im*fpkellfksnfllpr*inl*rfklw*fniwwwfir-
--

---mimvknvv*kihcdevcdldcgrgfeckirhdgskccvrserphppqhekcnkrcpp
gheckvdqhgkeccvvahrpppkcslrcpprhecrvnhfgeeccvkvhhdkcslrcppgh
eckvdqhgkeccvvahrpppkcslrcppkhecrinhfgeeccvksrndcltcedlxcakk
r

Frame B:
fnfifnxfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEECH
KSSECIVINYTPCXGEQKWACSKGTFDTCTYENSCYRNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAKIKECALLGKSCSFQKNSCSNPTSCCPGESICEGLSSGSSTSGGGSSG-
--

---*sw*kmlckrfivmkfvi*ivvevlnvkldmmvqnvvsvqkdhthhnmknvirdaxq
amnvklinmeknvallpidhhqnal*dvxqdmnvesttlvknvvlkfttinvh*dvhqam
nvklinmeknvallpidhhqnah*dvhqnmnvesitlvknavlkvemiv*lvktxtvqrk
g

Frame C:
silflxnsn*kknnn*ls*k*knnkgccf*kileyfl*yslyqnpmrrivikllmkknvi
nlrnvll*iihhvxvnknglalkvhlilvhmkivvieihpitkllkyqinasilmdllkl
qlqpsilvlmprlknvpy*vnhvvskrtlvqiqlpvaqvnqfvkv*alvvqhlvvvhqv-
--

---DHGKKCCVKDSL**sl*frlw*rf*m*n*t*wfkmlcpfrkttpttt*km**empxr
p*m*s*stwkrmlrccp*tttkmlfkmsxkt*m*sqplw*rmlc*sspr*mfiemstrp*
m*s*stwkrmlrccp*tttkmlikmstkt*m*nqslw*rmlc*k*k*lfnl*rpxlckek
v
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFE825 (SFE825Q) /CSM/SF/SFE8-B/SFE825Q.Seq.d/                       1877   0.0
AFI171 (AFI171Q) /CSM/AF/AFI1-C/AFI171Q.Seq.d/                       1015   0.0
SSF378 (SSF378Q) /CSM/SS/SSF3-D/SSF378Q.Seq.d/                       1013   0.0
SLH866 (SLH866Q) /CSM/SL/SLH8-C/SLH866Q.Seq.d/                       1013   0.0
SLG302 (SLG302Q) /CSM/SL/SLG3-A/SLG302Q.Seq.d/                       1013   0.0
SLG130 (SLG130Q) /CSM/SL/SLG1-B/SLG130Q.Seq.d/                       1013   0.0
SLE706 (SLE706Q) /CSM/SL/SLE7-A/SLE706Q.Seq.d/                       1013   0.0
SLB753 (SLB753Q) /CSM/SL/SLB7-C/SLB753Q.Seq.d/                       1013   0.0
SLB309 (SLB309Q) /CSM/SL/SLB3-A/SLB309Q.Seq.d/                       1013   0.0
SHE749 (SHE749Q) /CSM/SH/SHE7-C/SHE749Q.Seq.d/                       1013   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117267|AC117267.1 Dictyostelium discoideum chromosome 2 map 4767688-4790343 strain AX4, *** SEQUENCING IN PROGRESS ***.   967   0.0    8
U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.   967   0.0    6
AC115610|AC115610.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.   967   0.0    8
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.    66   2e-11  2
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    66   2e-11  10
X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.    66   3e-11  2
AC115685|AC115685.1 Dictyostelium discoideum chromosome 2 map 4718821-4752388 strain AX4, complete sequence.    66   7e-10  4
AC141955|AC141955.2 Rattus norvegicus clone CH230-418P10, WORKING DRAFT SEQUENCE, 29 unordered pieces.    48   0.17   4
AX348726|AX348726.1 Sequence 184 from Patent WO0202807.            32   0.19   5
AX344319|AX344319.1 Sequence 166 from Patent WO0200926.            32   0.19   5
dna update 2003.12.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   397   e-110
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   146   1e-33
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...   110   1e-22
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...   102   2e-20
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    76   1e-15
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        67   7e-14
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    68   9e-12
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    61   8e-08
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    58   6e-07
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    58   6e-07
protein update 2009. 5.24
PSORT 

 psg: 1.02  gvh: 0.68  alm: 0.51  top: 0.20  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for SFE825 is exc
5' end seq. ID SFE825F
5' end seq. 
>SFE825F.Seq
ATTCAATTTTATTTTTAATNAATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAA
AATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATT
CCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAAGAATGTCA
TAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGTGAACAAAAATGGGC
TTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATAGAAATTCATC
CAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAAT
TACAACTCCAACCGAGTATTCTTGTTCTGATGCCAAGATTAAAGAATGTGCCTTATTAGG
TAAATCATGTAGTTTCCAAAAGAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGA
ATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTG-
---------
Length of 5' end seq. 539
3' end seq. ID SFE825Z
3' end seq. 
>SFE825Z.Seq
----------ATGATCATGGTAAAAAATGTTGTGTAAAAGATTCATTGTGATGAAGTTTG
TGATTTAGATTGTGGTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTG
TGTCCGTTCAGAAAGACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCNCC
AGGCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACC
ACCACCAAAATGCTCTTTAAGATGTCCNCCAAGACATGAATGTAGAGTCAACCACTTTGG
TGAAGAATGTTGTGTTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCA
TGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACC
AAAATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGA
ATGCTGTGTTAAAAGTAGAAATGATTGTTTAACTTGTGAAGACCTANACTGTGCAAAGAA
AAGGTT
Length of 3' end seq. 536
Connected seq. ID SFE825P
Connected seq. 
>SFE825P.Seq
ATTCAATTTTATTTTTAATNAATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAA
AATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATT
CCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAAGAATGTCA
TAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGTGAACAAAAATGGGC
TTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATAGAAATTCATC
CAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAAT
TACAACTCCAACCGAGTATTCTTGTTCTGATGCCAAGATTAAAGAATGTGCCTTATTAGG
TAAATCATGTAGTTTCCAAAAGAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGA
ATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTG-
---------ATGATCATGGTAAAAAATGTTGTGTAAAAGATTCATTGTGATGAAGTTTGT
GATTTAGATTGTGGTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGT
GTCCGTTCAGAAAGACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCNCCA
GGCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCA
CCACCAAAATGCTCTTTAAGATGTCCNCCAAGACATGAATGTAGAGTCAACCACTTTGGT
GAAGAATGTTGTGTTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCAT
GAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCA
AAATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAA
TGCTGTGTTAAAAGTAGAAATGATTGTTTAACTTGTGAAGACCTANACTGTGCAAAGAAA
AGGTT
Length of connected seq. 1075
Full length Seq ID -
Full length Seq. -
Length of full length seq. -