>AFI220 (AFI220Q) /CSM/AF/AFI2-A/AFI220Q.Seq.d/
XXXXXXXXXXATGATTGGGAATTACTTCCATCAAANCAAATCAAAGATCCAAAACAATCC
AAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCC
GGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGG
AATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGT
GAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTA
ATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCT
ATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACC
GACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCC
GAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGAC
GAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAA
GAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAA
TCAACCAAAAAAGTTGATCATGACGAACTTTAAATGAAATAAATAAATAATAAAAAA

---DWELLPSXQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWN
EEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAI
GFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADE
KLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVDHDEL*mk*innkk

Frame A:
---*lgitsikxnqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle
*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*tlpl**srcy
rfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrqs*rekes*r
kiscrksc*krsrrs**rrrrsr*rrfs*n**qkrrsqkinqks*s*rtlneink**k


Frame B:
---DWELLPSXQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWN
EEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAI
GFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADE
KLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVDHDEL*mk*innkk


Frame C:
---ignyfhqxkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliqklsnqrigm
kkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmnstslmisvls
vsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppklkrerkltk
n*lpkkllkkkpkklmkkkkkslkki*lklmtkkkksknqpkklimtnfk*nk*iik

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                        991   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                        991   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        991   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        991   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                        991   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                        991   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                        991   0.0
SFK591 (SFK591Q) /CSM/SF/SFK5-D/SFK591Q.Seq.d/                        991   0.0
SFJ301 (SFJ301Q) /CSM/SF/SFJ3-A/SFJ301Q.Seq.d/                        991   0.0
SFI129 (SFI129Q) /CSM/SF/SFI1-B/SFI129Q.Seq.d/                        991   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   991   0.0    2
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-15  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   5e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   3e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74   3e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74   3e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74   3e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74   3e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   3e-09  1
BT013428|BT013428.1 Lycopersicon esculentum clone 132068F, mRNA sequence.    74   3e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   308   1e-82
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   194   2e-48
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   192   1e-47
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   190   4e-47
AK230218_1(AK230218|pid:none) Arabidopsis thaliana mRNA for hypo...   188   1e-46
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   187   3e-46
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   187   3e-46
AC149080_5(AC149080|pid:none) Medicago truncatula clone mth2-75j...   186   8e-46
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   185   1e-45
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   184   2e-45

 psg: 0.75  gvh: 0.31  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.08  mip: 0.00
 nuc: 0.06  erl: 1.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFI220 is nuc

>AFI220Z.Seq
----------ATGATTGGGAATTACTTCCATCAAANCAAATCAAAGATCCAAAACAATCC
AAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCC
GGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGG
AATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGT
GAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTA
ATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCT
ATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACC
GACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCC
GAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGAC
GAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAA
GAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAA
TCAACCAAAAAAGTTGATCATGACGAACTTTAAATGAAATAAATAAATAATAAAAAA