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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFE776
Library SF
(Link to library)
Clone ID SFE776
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16296-1
Original site URL
Representative seq. ID SFE776P
(Link to Original site)
Representative DNA sequence 
>SFE776 (SFE776Q) /CSM/SF/SFE7-D/SFE776Q.Seq.d/
ATATTATATATGAAACATTAAAAAAATGAAAGTTATATTATTATTTGTTTTAGCTGTTTT
TACTGTTTTTGTTTCAAGTAGAGGAATTCCATTAGAAGAACAAAGTCAATTCCTTGAATT
TCAAGATAAATTCAATAAAAAATATTCACATGAAGAATATTTGGAAAGATTTGAAATTTT
TAAAAGCAATTTAGGAAAAATTGAAGAATTAAATCTAATAGCCATTAATCACAAAGCTGA
TACTAAATTTGGTGTAAACAAGTTTGCAGATCTTTCCAGTGACGAATTTAAAAATTATTA
TTTAAATAATAAGGAAGCAATATTCACTGATGACCTTCCAGTTGCTGATTATCTTGATGA
TGAATTCATTAATTCAATTCCAACTGCATTTGATTGGAGAACTAGAGGTGCTGTTACACC
TGTAAAAAATCAAGGTCAATGTGGTAGTTGTTGGTCATTTTCAACTACTGGTAATGTTGA
GGGACAACATTTCATTAGTCAGAATAAATTAGTTTCATTATCAGAGCAAAACTTGGTAGA
TTGTGATCATGAGTGTATGGAATATGAAGGTGAACAAGCTTGTGGTGAGGGTTGTAATGG
TGGTCXXXXXXXXXXTACACTGCTGAAACAGGTACACAATGTACCTTTAACTCTGCCAAT
ATTGGTGCAAAGATTTCCAATTTTACAATGATCCCAAAGAATGAAACTGTAATGGCTGGG
TACATCGTTAGTACTGGACCACTCGCAATTGCTGCTGATGCTGTTGAGTGGCAATTTTAT
ATTGGTGGTGTATTTGATATTCCATGTAATCCAAATTCACTTGATCATGGTATTTTAATT
GTTGGTTACTCTGCTAAAAATACAATTTTCCGTAAAAATATGCCATATTGGATTGTAAAG
AATTCTTGGGGTGCAGATTGGGGAGAACAAGGATACATTTATTTAAGAAGAGGAAAGAAT
ACATGTGGTGTATCAAACTTTGTTTCAACTAGTATAATTTAAATTTATACCAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
yyi*NIKKMKVILLFVLAVFTVFVSSRGIPLEEQSQFLEFQDKFNKKYSHEEYLERFEIF
KSNLGKIEELNLIAINHKADTKFGVNKFADLSSDEFKNYYLNNKEAIFTDDLPVADYLDD
EFINSIPTAFDWRTRGAVTPVKNQGQCGSCWSFSTTGNVEGQHFISQNKLVSLSEQNLVD
CDHECMEYEGEQACGEGCNGG---

---YTAETGTQCTFNSANIGAKISNFTMIPKNETVMAGYIVSTGPLAIAADAVEWQFYIG
GVFDIPCNPNSLDHGILIVGYSAKNTIFRKNMPYWIVKNSWGADWGEQGYIYLRRGKNTC
GVSNFVSTSII*iytk
Translated Amino Acid sequence (All Frames) 
Frame A:
ilymkh*knesyiiicfscfycfcfk*rnsirrtksip*isr*iq*kift*rifgki*nf
*kqfrkn*riksnsh*sqs*y*iwckqvcrsfq*ri*kllfk**gsnih**pssc*ls**
*ih*fnsnci*len*rccytckksrsmw*llvifnyw*c*gttfh*se*isfiiraklgr
l*s*vygi*r*tslw*gl*ww---

---YTAETGTQCTFNSANIGAKISNFTMIPKNETVMAGYIVSTGPLAIAADAVEWQFYIG
GVFDIPCNPNSLDHGILIVGYSAKNTIFRKNMPYWIVKNSWGADWGEQGYIYLRRGKNTC
GVSNFVSTSII*iytk

Frame B:
yyi*NIKKMKVILLFVLAVFTVFVSSRGIPLEEQSQFLEFQDKFNKKYSHEEYLERFEIF
KSNLGKIEELNLIAINHKADTKFGVNKFADLSSDEFKNYYLNNKEAIFTDDLPVADYLDD
EFINSIPTAFDWRTRGAVTPVKNQGQCGSCWSFSTTGNVEGQHFISQNKLVSLSEQNLVD
CDHECMEYEGEQACGEGCNGG---

---tllkqvhnvpltlpilvqrfpilq*sqrmkl*wlgtslvldhsqlllmllsgnfilv
vylifhviqihlimvf*llvtllkiqfsvkichigl*rilgvqigenkdtfi*eeerihv
vyqtlfqlv*fkfip

Frame C:
iiyetlkk*klyyylf*lfllflfqveefh*knkvnslnfkinsiknihmkniwkdlkfl
kai*eklkn*i**plitklilnlv*tslqifpvtnlkiii*iirkqyslmtfqlliilmm
nsliqfqlhligelevllhl*kikvnvvvvghfqllvmlrdnislvrin*fhyqsktw*i
vimsvwnmkvnklvvrvvmvv---

---hc*nrytmyl*lcqywckdfqfyndpke*ncngwvhr*ywttrncc*cc*vailyww
ci*ysm*skft*swyfncwllc*kynfp*kyaildckeflgcrlgrtrihlfkkrkeymw
ciklcfn*ynlnlyq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFE776 (SFE776Q) /CSM/SF/SFE7-D/SFE776Q.Seq.d/                       1804   0.0
VFF582 (VFF582Q) /CSM/VF/VFF5-D/VFF582Q.Seq.d/                       1045   0.0
VFA268 (VFA268Q) /CSM/VF/VFA2-C/VFA268Q.Seq.d/                       1045   0.0
SFJ881 (SFJ881Q) /CSM/SF/SFJ8-D/SFJ881Q.Seq.d/                       1045   0.0
SFF369 (SFF369Q) /CSM/SF/SFF3-C/SFF369Q.Seq.d/                       1045   0.0
SFE852 (SFE852Q) /CSM/SF/SFE8-C/SFE852Q.Seq.d/                       1045   0.0
SFA643 (SFA643Q) /CSM/SF/SFA6-B/SFA643Q.Seq.d/                       1045   0.0
CHD895 (CHD895Q) /CSM/CH/CHD8-D/CHD895Q.Seq.d/                       1045   0.0
AFN337 (AFN337Q) /CSM/AF/AFN3-B/AFN337Q.Seq.d/                       1045   0.0
AFK421 (AFK421Q) /CSM/AF/AFK4-A/AFK421Q.Seq.d/                       1045   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X02407|X02407.1 D.discoideum mRNA for cysteine proteinase 1.     1021   0.0    3
AJ510164|AJ510164.1 Cloning vector pDXA-3strep.                   192   3e-46  2
X85119|X85119.1 Artificial sequences cloning vector DNA pDXA-3H.   192   3e-46  2
X85122|X85122.1 Artificial sequences cloning vector DNA pDXA-HY.   192   3e-46  2
AJ510165|AJ510165.1 Cloning vector pDXA-3FLAG.                    192   3e-46  2
X85118|X85118.1 Artificial sequences cloning vector DNA pDXA-3C.   192   3e-46  2
X85123|X85123.1 Artificial sequences cloning vector DNA pDXA-HC.   192   3e-46  2
AF269236|AF269236.1 Cloning vector pDXA-FLAG, complete sequence.   192   3e-46  2
X85120|X85120.1 Artificial sequences cloning vector DNA pDXD-3H.   192   3e-46  2
AJ510166|AJ510166.1 Cloning vector pDXA-GST.                      192   3e-46  2
dna update 2003.12.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04988) RecName: Full=Cysteine proteinase 1;          EC=3.4.22...   369   e-175
U42758_1(U42758|pid:none) Naegleria fowleri cysteine proteinase ...   166   7e-70
FJ609256_1(FJ609256|pid:none) Solanum lycopersicum cysteine prot...   147   5e-64
AJ580823_4(AJ580823|pid:none) Lotus corniculatus var. japonicus ...   145   8e-63
AJ580823_3(AJ580823|pid:none) Lotus corniculatus var. japonicus ...   144   8e-63
BT071299_1(BT071299|pid:none) Picea sitchensis clone WS02822_C20...   144   1e-62
EF144276_1(EF144276|pid:none) Populus trichocarpa clone PX0015_D...   152   2e-62
AC149637_11(AC149637|pid:none) Medicago truncatula clone mth2-18...   148   4e-62
AF138264_1(AF138264|pid:none) Ipomoea batatas papain-like cystei...   143   1e-61
AY800120_1(AY800120|pid:none) Populus tomentosa cysteine protein...   148   2e-61
protein update 2009. 5.24
PSORT 

 psg: 0.99  gvh: 0.64  alm: 0.20  top: 0.13  tms: 0.07  mit: 0.30  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: Golgi
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for SFE776 is end
5' end seq. ID SFE776F
5' end seq. 
>SFE776F.Seq
ATATTATATATGAAACATTAAAAAAATGAAAGTTATATTATTATTTGTTTTAGCTGTTTT
TACTGTTTTTGTTTCAAGTAGAGGAATTCCATTAGAAGAACAAAGTCAATTCCTTGAATT
TCAAGATAAATTCAATAAAAAATATTCACATGAAGAATATTTGGAAAGATTTGAAATTTT
TAAAAGCAATTTAGGAAAAATTGAAGAATTAAATCTAATAGCCATTAATCACAAAGCTGA
TACTAAATTTGGTGTAAACAAGTTTGCAGATCTTTCCAGTGACGAATTTAAAAATTATTA
TTTAAATAATAAGGAAGCAATATTCACTGATGACCTTCCAGTTGCTGATTATCTTGATGA
TGAATTCATTAATTCAATTCCAACTGCATTTGATTGGAGAACTAGAGGTGCTGTTACACC
TGTAAAAAATCAAGGTCAATGTGGTAGTTGTTGGTCATTTTCAACTACTGGTAATGTTGA
GGGACAACATTTCATTAGTCAGAATAAATTAGTTTCATTATCAGAGCAAAACTTGGTAGA
TTGTGATCATGAGTGTATGGAATATGAAGGTGAACAAGCTTGTGGTGAGGGTTGTAATGG
TGGTC----------
Length of 5' end seq. 605
3' end seq. ID SFE776Z
3' end seq. 
>SFE776Z.Seq
----------TACACTGCTGAAACAGGTACACAATGTACCTTTAACTCTGCCAATATTGG
TGCAAAGATTTCCAATTTTACAATGATCCCAAAGAATGAAACTGTAATGGCTGGGTACAT
CGTTAGTACTGGACCACTCGCAATTGCTGCTGATGCTGTTGAGTGGCAATTTTATATTGG
TGGTGTATTTGATATTCCATGTAATCCAAATTCACTTGATCATGGTATTTTAATTGTTGG
TTACTCTGCTAAAAATACAATTTTCCGTAAAAATATGCCATATTGGATTGTAAAGAATTC
TTGGGGTGCAGATTGGGGAGAACAAGGATACATTTATTTAAGAAGAGGAAAGAATACATG
TGGTGTATCAAACTTTGTTTCAACTAGTATAATTTAAATTTATACCAAA
Length of 3' end seq. 399
Connected seq. ID SFE776P
Connected seq. 
>SFE776P.Seq
ATATTATATATGAAACATTAAAAAAATGAAAGTTATATTATTATTTGTTTTAGCTGTTTT
TACTGTTTTTGTTTCAAGTAGAGGAATTCCATTAGAAGAACAAAGTCAATTCCTTGAATT
TCAAGATAAATTCAATAAAAAATATTCACATGAAGAATATTTGGAAAGATTTGAAATTTT
TAAAAGCAATTTAGGAAAAATTGAAGAATTAAATCTAATAGCCATTAATCACAAAGCTGA
TACTAAATTTGGTGTAAACAAGTTTGCAGATCTTTCCAGTGACGAATTTAAAAATTATTA
TTTAAATAATAAGGAAGCAATATTCACTGATGACCTTCCAGTTGCTGATTATCTTGATGA
TGAATTCATTAATTCAATTCCAACTGCATTTGATTGGAGAACTAGAGGTGCTGTTACACC
TGTAAAAAATCAAGGTCAATGTGGTAGTTGTTGGTCATTTTCAACTACTGGTAATGTTGA
GGGACAACATTTCATTAGTCAGAATAAATTAGTTTCATTATCAGAGCAAAACTTGGTAGA
TTGTGATCATGAGTGTATGGAATATGAAGGTGAACAAGCTTGTGGTGAGGGTTGTAATGG
TGGTC----------TACACTGCTGAAACAGGTACACAATGTACCTTTAACTCTGCCAAT
ATTGGTGCAAAGATTTCCAATTTTACAATGATCCCAAAGAATGAAACTGTAATGGCTGGG
TACATCGTTAGTACTGGACCACTCGCAATTGCTGCTGATGCTGTTGAGTGGCAATTTTAT
ATTGGTGGTGTATTTGATATTCCATGTAATCCAAATTCACTTGATCATGGTATTTTAATT
GTTGGTTACTCTGCTAAAAATACAATTTTCCGTAAAAATATGCCATATTGGATTGTAAAG
AATTCTTGGGGTGCAGATTGGGGAGAACAAGGATACATTTATTTAAGAAGAGGAAAGAAT
ACATGTGGTGTATCAAACTTTGTTTCAACTAGTATAATTTAAATTTATACCAAA
Length of connected seq. 1004
Full length Seq ID -
Full length Seq. -
Length of full length seq. -