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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI217
Library AF
(Link to library)
Clone ID AFI217
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16259-1
Original site URL
Representative seq. ID AFI217P
(Link to Original site)
Representative DNA sequence 
>AFI217 (AFI217Q) /CSM/AF/AFI2-A/AFI217Q.Seq.d/
TCAGTTATTCGTGTTGCAACAGGTGGCTCTCCAACCCCATTTGACCTTTCTGTTGTAGGT
NTTGATGATGAAAAGGAATCTTATTTAACATTTAACTTACCAACAAACTCATCAAAGAGT
GCAGTTACAGTCTATGATGGTCAAGATACTTCAAATAATTTTGATGTCACACCAACACCA
TTTATTTCTAATTTAAAAGCTCTCCCATCAACAGATGGTCAAGAAATTAATTTCATCGGT
GGTTATCTTGATTCAAAAGCAAAAGCAACTATTTCCCTTTTGGATGCAAGAAATCAAAGT
GTTGATTGTTCAGATTTCTTAATTAGTCCAGATTCATCATCTGCTACTTGTAAACTTGCA
CCAGGATGTGGTTCAATTAAATCTACAATTGTTATTGGTGAAAACTCAATTCCACTAGAT
TTTAACTACCAATCCCCAAGTTCTTTAGTCGCAAAACAAATGTCTGATAATAAATTACAA
ATTAGTGGCAAAAACCTTGGTCCAAATTCAAAATATATAAAGTTAGACATGAACGGAAAC
GAACTGTTAGCTTCCTXXXXXXXXXXAAACTCAGACTCTGACCAACTTGATGTCGAGCTT
CAACCAGTTGTCAAATCAATTACTCAATCACAAACCTCTGGATCAACTGTGACAATTAAA
GGTTCATTCTTTAATAAAAAGGACACTGGTTTGAAAATTATGGTTGGAAATCATTCATGT
GATTCTTTGACTATTGTAGATACCCAAACACTTACTTGCTCTGCTCCACCAGGAAGTGGT
ATTAAAAATGCTGTTTCATTCATGGTTAAAGATGCTGAATCAAAATTATCACCACCATTG
TTTTTAGATTATAGTCCACCAACCATTATGTCATCAACAAGTGTAAAACAAGTTGAAGGT
GGTCAAGTTACTATTTATGGTACCAATTTTGAAAATACTTTGATTACAGTAAATGTTGGA
AGTAAATCATGTGAAGATGTTTCTGTAATAGATTCAACCAAACTTACTTGTTCAATTTCA
AAATCAACAATAGATCCTTCATTAACAAATAAGAAATTGGCCGTAAATGTTACTGTAAAT
GGTCAATCTGCTAATGGTGAAGTATTTGAATATGATAATGAAGCACTCAATACTGAAACT
CCTAAACCAACATTAGCACCAACTATAAAACCAACTGAAAAACCAACTGAAAAACCAACT
GAAAAACCAACAACATCTCCAATAGATAAACACATNAAAATAGTTCTTCTAAAATTTCTA
TTCATTATTTNATTTTCTCAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
SVIRVATGGSPTPFDLSVVGXDDEKESYLTFNLPTNSSKSAVTVYDGQDTSNNFDVTPTP
FISNLKALPSTDGQEINFIGGYLDSKAKATISLLDARNQSVDCSDFLISPDSSSATCKLA
PGCGSIKSTIVIGENSIPLDFNYQSPSSLVAKQMSDNKLQISGKNLGPNSKYIKLDMNGN
ELLAS---

---NSDSDQLDVELQPVVKSITQSQTSGSTVTIKGSFFNKKDTGLKIMVGNHSCDSLTIV
DTQTLTCSAPPGSGIKNAVSFMVKDAESKLSPPLFLDYSPPTIMSSTSVKQVEGGQVTIY
GTNFENTLITVNVGSKSCEDVSVIDSTKLTCSISKSTIDPSLTNKKLAVNVTVNGQSANG
EVFEYDNEALNTETPKPTLAPTIKPTEKPTEKPTEKPTTSPIDKHXKIVLLKFLFIIXFS
H
Translated Amino Acid sequence (All Frames) 
Frame A:
SVIRVATGGSPTPFDLSVVGXDDEKESYLTFNLPTNSSKSAVTVYDGQDTSNNFDVTPTP
FISNLKALPSTDGQEINFIGGYLDSKAKATISLLDARNQSVDCSDFLISPDSSSATCKLA
PGCGSIKSTIVIGENSIPLDFNYQSPSSLVAKQMSDNKLQISGKNLGPNSKYIKLDMNGN
ELLAS---

---klrl*pt*crastscqinysitnlwincdn*rfil**kghwfenygwksfm*ffdyc
rypntyllcstrkwy*kccfihg*rc*ikiittivfrl*stnhyvinkckts*rwssyyl
wyqf*kyfdyskcwk*im*rcfcnrfnqtylfnfkinnrsfink*eigrkcyckwsic*w
*si*i***stqy*ns*tnistnyktn*ktn*ktn*ktnnisnr*thxnssskisihyxif
s

Frame B:
qlfvlqqvalqphltfll*vlmmkrnli*hltyqqthqrvqlqsmmvkilqiilmshqhh
lfli*klshqqmvkklissvviliqkqkqlfpfwmqeikvlivqis*lvqihhlllvnlh
qdvvqlnlqlllvktqfh*ilttnpqvl*sqnkcliinyklvaktlvqiqni*s*t*tet
nc*lp---

---NSDSDQLDVELQPVVKSITQSQTSGSTVTIKGSFFNKKDTGLKIMVGNHSCDSLTIV
DTQTLTCSAPPGSGIKNAVSFMVKDAESKLSPPLFLDYSPPTIMSSTSVKQVEGGQVTIY
GTNFENTLITVNVGSKSCEDVSVIDSTKLTCSISKSTIDPSLTNKKLAVNVTVNGQSANG
EVFEYDNEALNTETPKPTLAPTIKPTEKPTEKPTEKPTTSPIDKHXKIVLLKFLFIIXFS
H

Frame C:
sysccnrwlsnpi*pfccrx***kgilfni*ltnklikecsysl*wsryfk*f*chtnti
yf*fksspinrwsrn*fhrwls*fksksnyfpfgckkskc*lfrfln*srfiicyl*tct
rmwfn*iyncyw*klnstrf*lpipkffsrktnv***itn*wqkpwskfkiykvrherkr
tvsf---

---tqtltnlmssfnqlsnqllnhkpldql*qlkvhslikrtlv*klwleiihvil*ll*
ipkhllallhqevvlkmlfhswlkmlnqnyhhhcf*iivhqplchqqv*nklkvvkllfm
vpilkil*lq*mlevnhvkmfl**iqpnllvqfqnqq*ilh*qirnwp*mll*mvnllmv
kylnmimkhsilkllnqh*hql*nqlknqlknqlknqqhlq*intxk*ff*nfyslfxfl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL869 (SHL869Q) /CSM/SH/SHL8-C/SHL869Q.Seq.d/                       1170   0.0
SHK779 (SHK779Q) /CSM/SH/SHK7-D/SHK779Q.Seq.d/                       1170   0.0
SHK674 (SHK674Q) /CSM/SH/SHK6-D/SHK674Q.Seq.d/                       1170   0.0
SHK612 (SHK612Q) /CSM/SH/SHK6-A/SHK612Q.Seq.d/                       1170   0.0
SHK454 (SHK454Q) /CSM/SH/SHK4-C/SHK454Q.Seq.d/                       1170   0.0
SHK336 (SHK336Q) /CSM/SH/SHK3-B/SHK336Q.Seq.d/                       1170   0.0
SHG859 (SHG859Q) /CSM/SH/SHG8-C/SHG859Q.Seq.d/                       1170   0.0
SHG695 (SHG695Q) /CSM/SH/SHG6-D/SHG695Q.Seq.d/                       1170   0.0
SHG451 (SHG451Q) /CSM/SH/SHG4-C/SHG451Q.Seq.d/                       1170   0.0
SHG394 (SHG394Q) /CSM/SH/SHG3-D/SHG394Q.Seq.d/                       1170   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    32   0.002  17
AL445564|AL445564.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 2/3.    38   0.004  16
AE017338|AE017338.1 Mycoplasma mobile 163K section 2 of 3 of the complete genome.    36   0.011  13
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    38   0.015  14
AC135798|AC135798.25 Medicago truncatula clone mth2-31c16, WORKING DRAFT SEQUENCE, 11 unordered pieces.    36   0.029  11
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    34   0.068  24
AC015446|AC015446.4 Arabidopsis thaliana chromosome I BAC F12G12 genomic sequence, complete sequence.    38   0.080  8
AC139806|AC139806.1 Homo sapiens chromosome 5 clone RP11-1357D4, WORKING DRAFT SEQUENCE, 7 unordered pieces.    34   0.12   11
AP006426|AP006426.1 Lotus corniculatus var. japonicus genomic DNA, chromosome 1, clone:LjT02I08, TM0316.    36   0.13   8
BX842644|BX842644.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 3/4.    34   0.17   16
dna update 2004. 6.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117076_23(AC117076|pid:none) Dictyostelium discoideum chromoso...   109   2e-22
FJ374909_1(FJ374909|pid:none) Dictyostelium discoideum strain QS...    77   1e-12
FJ374902_1(FJ374902|pid:none) Dictyostelium discoideum strain QS...    76   3e-12
FJ374903_1(FJ374903|pid:none) Dictyostelium discoideum strain QS...    74   8e-12
FJ374905_1(FJ374905|pid:none) Dictyostelium discoideum strain QS...    74   1e-11
FJ374919_1(FJ374919|pid:none) Dictyostelium discoideum strain QS...    74   1e-11
FJ374895_1(FJ374895|pid:none) Dictyostelium discoideum strain QS...    73   2e-11
FJ374893_1(FJ374893|pid:none) Dictyostelium discoideum strain QS...    73   2e-11
FJ374900_1(FJ374900|pid:none) Dictyostelium discoideum strain QS...    73   2e-11
FJ374971_1(FJ374971|pid:none) Dictyostelium discoideum strain AX...    73   2e-11
protein update 2009. 4.22
PSORT 

 psg: 0.85  gvh: 0.34  alm: 0.40  top: 0.13  tms: 0.00  mit: 0.26  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFI217 is cyt
5' end seq. ID AFI217F
5' end seq. 
>AFI217F.Seq
TCAGTTATTCGTGTTGCAACAGGTGGCTCTCCAACCCCATTTGACCTTTCTGTTGTAGGT
NTTGATGATGAAAAGGAATCTTATTTAACATTTAACTTACCAACAAACTCATCAAAGAGT
GCAGTTACAGTCTATGATGGTCAAGATACTTCAAATAATTTTGATGTCACACCAACACCA
TTTATTTCTAATTTAAAAGCTCTCCCATCAACAGATGGTCAAGAAATTAATTTCATCGGT
GGTTATCTTGATTCAAAAGCAAAAGCAACTATTTCCCTTTTGGATGCAAGAAATCAAAGT
GTTGATTGTTCAGATTTCTTAATTAGTCCAGATTCATCATCTGCTACTTGTAAACTTGCA
CCAGGATGTGGTTCAATTAAATCTACAATTGTTATTGGTGAAAACTCAATTCCACTAGAT
TTTAACTACCAATCCCCAAGTTCTTTAGTCGCAAAACAAATGTCTGATAATAAATTACAA
ATTAGTGGCAAAAACCTTGGTCCAAATTCAAAATATATAAAGTTAGACATGAACGGAAAC
GAACTGTTAGCTTCCT----------
Length of 5' end seq. 556
3' end seq. ID AFI217Z
3' end seq. 
>AFI217Z.Seq
----------AAACTCAGACTCTGACCAACTTGATGTCGAGCTTCAACCAGTTGTCAAAT
CAATTACTCAATCACAAACCTCTGGATCAACTGTGACAATTAAAGGTTCATTCTTTAATA
AAAAGGACACTGGTTTGAAAATTATGGTTGGAAATCATTCATGTGATTCTTTGACTATTG
TAGATACCCAAACACTTACTTGCTCTGCTCCACCAGGAAGTGGTATTAAAAATGCTGTTT
CATTCATGGTTAAAGATGCTGAATCAAAATTATCACCACCATTGTTTTTAGATTATAGTC
CACCAACCATTATGTCATCAACAAGTGTAAAACAAGTTGAAGGTGGTCAAGTTACTATTT
ATGGTACCAATTTTGAAAATACTTTGATTACAGTAAATGTTGGAAGTAAATCATGTGAAG
ATGTTTCTGTAATAGATTCAACCAAACTTACTTGTTCAATTTCAAAATCAACAATAGATC
CTTCATTAACAAATAAGAAATTGGCCGTAAATGTTACTGTAAATGGTCAATCTGCTAATG
GTGAAGTATTTGAATATGATAATGAAGCACTCAATACTGAAACTCCTAAACCAACATTAG
CACCAACTATAAAACCAACTGAAAAACCAACTGAAAAACCAACTGAAAAACCAACAACAT
CTCCAATAGATAAACACATNAAAATAGTTCTTCTAAAATTTCTATTCATTATTTNATTTT
CTCAT
Length of 3' end seq. 715
Connected seq. ID AFI217P
Connected seq. 
>AFI217P.Seq
TCAGTTATTCGTGTTGCAACAGGTGGCTCTCCAACCCCATTTGACCTTTCTGTTGTAGGT
NTTGATGATGAAAAGGAATCTTATTTAACATTTAACTTACCAACAAACTCATCAAAGAGT
GCAGTTACAGTCTATGATGGTCAAGATACTTCAAATAATTTTGATGTCACACCAACACCA
TTTATTTCTAATTTAAAAGCTCTCCCATCAACAGATGGTCAAGAAATTAATTTCATCGGT
GGTTATCTTGATTCAAAAGCAAAAGCAACTATTTCCCTTTTGGATGCAAGAAATCAAAGT
GTTGATTGTTCAGATTTCTTAATTAGTCCAGATTCATCATCTGCTACTTGTAAACTTGCA
CCAGGATGTGGTTCAATTAAATCTACAATTGTTATTGGTGAAAACTCAATTCCACTAGAT
TTTAACTACCAATCCCCAAGTTCTTTAGTCGCAAAACAAATGTCTGATAATAAATTACAA
ATTAGTGGCAAAAACCTTGGTCCAAATTCAAAATATATAAAGTTAGACATGAACGGAAAC
GAACTGTTAGCTTCCT----------AAACTCAGACTCTGACCAACTTGATGTCGAGCTT
CAACCAGTTGTCAAATCAATTACTCAATCACAAACCTCTGGATCAACTGTGACAATTAAA
GGTTCATTCTTTAATAAAAAGGACACTGGTTTGAAAATTATGGTTGGAAATCATTCATGT
GATTCTTTGACTATTGTAGATACCCAAACACTTACTTGCTCTGCTCCACCAGGAAGTGGT
ATTAAAAATGCTGTTTCATTCATGGTTAAAGATGCTGAATCAAAATTATCACCACCATTG
TTTTTAGATTATAGTCCACCAACCATTATGTCATCAACAAGTGTAAAACAAGTTGAAGGT
GGTCAAGTTACTATTTATGGTACCAATTTTGAAAATACTTTGATTACAGTAAATGTTGGA
AGTAAATCATGTGAAGATGTTTCTGTAATAGATTCAACCAAACTTACTTGTTCAATTTCA
AAATCAACAATAGATCCTTCATTAACAAATAAGAAATTGGCCGTAAATGTTACTGTAAAT
GGTCAATCTGCTAATGGTGAAGTATTTGAATATGATAATGAAGCACTCAATACTGAAACT
CCTAAACCAACATTAGCACCAACTATAAAACCAACTGAAAAACCAACTGAAAAACCAACT
GAAAAACCAACAACATCTCCAATAGATAAACACATNAAAATAGTTCTTCTAAAATTTCTA
TTCATTATTTNATTTTCTCAT
Length of connected seq. 1271
Full length Seq ID -
Full length Seq. -
Length of full length seq. -