>SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/
CACTGTTGGCCTACTGGATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAAT
GAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGT
TGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCC
ATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAG
ATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGC
CGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGA
TGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGT
TTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGC
AGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAA
TGAAGCCTATCGTATGGGTTCAGAAGXXXXXXXXXXGTTTATCATAACTTAAAGAATGTC
ATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCA
CCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGT
TACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAAT
GGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAA
AAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGAT
CCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAA
ATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAG
AAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATT
CGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGT
GAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAA
ACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAAC
GAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTAC
TCATAATATGNAAACAAAAC

TVGLLDFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVP
SGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLD
GTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHA
GNKLAMQEFMILPVGAKDFNEAYRMGSE---

---VYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGM
DCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWES
YTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNA
SWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNY
AGLTFRKF*fys*yxnk

Frame A:
hcwptgfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscss
ircinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsr
wypk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftc
r**isyariydftswc*rfq*slsygfr---

---VYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGM
DCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWES
YTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNA
SWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNY
AGLTFRKF*fys*yxnk

Frame B:
TVGLLDFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVP
SGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLD
GTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHA
GNKLAMQEFMILPVGAKDFNEAYRMGSE---

---fiit*rmssvvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvw
ivlplnsklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignh
tln*qhqlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmh
hgvswlaivqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vktitm
lv*lsvnfnfthnmxtk

Frame C:
llaywiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfh
qvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sism
vpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmq
vin*lcknl*fyqlvlkismkpivwvqk---

---ls*lkechqw*irsrcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyg
lccl*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgii
h*insis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkeci
mgchg*psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*kp*lc
wfnfp*ililliixkqn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       2656   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1495   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1485   0.0
AFC723 (AFC723Q) /CSM/AF/AFC7-A/AFC723Q.Seq.d/                       1483   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1481   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1475   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1473   0.0
SFB722 (SFB722Q) /CSM/SF/SFB7-A/SFB722Q.Seq.d/                       1473   0.0
CHB226 (CHB226Q) /CSM/CH/CHB2-B/CHB226Q.Seq.d/                       1473   0.0
AFG692 (AFG692Q) /CSM/AF/AFG6-D/AFG692Q.Seq.d/                       1473   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X80474|X80474.1 N.frontalis enolase gene.                          64   1e-76  13
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   3e-72  11
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-62  10
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-62  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   1e-62  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   1e-60  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   1e-53  5
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66   3e-49  7
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121   3e-48  5
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   6e-47  10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   492   0.0
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   343   e-163
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   342   e-162
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   361   e-162
(Q43130) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   360   e-162
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   357   e-162
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   354   e-162
BC071359_1(BC071359|pid:none) Danio rerio enolase 1, (alpha), mR...   339   e-160
BC092869_1(BC092869|pid:none) Danio rerio enolase 3, (beta, musc...   343   e-159
AY398342_1(AY398342|pid:none) Danio rerio clone RK122A2D02 enola...   342   e-159

 psg: 0.75  gvh: 0.46  alm: 0.31  top: 0.67  tms: 0.07  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for SFE744 is cyt

>SFE744F.Seq
CACTGTTGGCCTACTGGATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAAT
GAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGT
TGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCC
ATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAG
ATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGC
CGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGA
TGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGT
TTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGC
AGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAA
TGAAGCCTATCGTATGGGTTCAGAAG----------

>SFE744Z.Seq
----------GTTTATCATAACTTAAAGAATGTCATCAGTGGTAGATACGGTCAAGATGC
TATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCT
TGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTAT
GGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAA
AAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATT
CATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATC
ATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTAC
CAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAA
AGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGC
ATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGA
TTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAG
ATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTA
TGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATATGNAAACAAAAC

>SFE744P.Seq
CACTGTTGGCCTACTGGATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAAT
GAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGT
TGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCC
ATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAG
ATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGC
CGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGA
TGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGT
TTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGC
AGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAA
TGAAGCCTATCGTATGGGTTCAGAAG----------GTTTATCATAACTTAAAGAATGTC
ATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCA
CCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGT
TACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAAT
GGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAA
AAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGAT
CCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAA
ATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAG
AAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATT
CGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGT
GAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAA
ACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAAC
GAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTAC
TCATAATATGNAAACAAAAC