>AFI215 (AFI215Q) /CSM/AF/AFI2-A/AFI215Q.Seq.d/
GGCCTACTGGTAAAAAAAAAAATCATCCATATTTATAATTATATCTTTTTTGTTTAATAA
AAAAGAAAGAATNCCANCNTGAGACTTTTATTGGGTTTAATTTTCTTAGTTTTTGTTTTC
AATTTTGCATTATCAACAGTTCATTTCAAAGATNCCTTTGATAACGATTGGGAATCAAGA
TGGGTTGTTAGNGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCA
GGTAAATGGTTTGGNGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTC
TACNCTGTATCAGCTAAATTCCCAAGTTTCAGTANCAAAGGTAAAGATTTAGTTCTCCAA
TACACTGTAAAGAATGANCAAAAAGTTGATTGNGGNGGTTCATACATCAAGTTATTACCA
TCCAAATTAGATCAATCAGCATTCGATGGNGAATCTGAATATTCCATTATGTTTGGNCCA
GATGTTTGNGGNGCCTCAAAGAGAXXXXXXXXXXAGAGATCCAAGCAGGTAACCTCGCTG
ATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCG
ATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACG
ATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAG
ACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAG
CAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACC
CAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCG
AATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCG
AAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAA
TGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAG
CTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCG
CTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAA
AAGTNATCANACGAACTTTAAANAAATAAATAAANA

gllvkkkiihiynyiffv**KRKNXXXRLLLGLIFLVFVFNFALSTVHFKDXFDNDWESR
WVVXDWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYXVSAKFPSFSXKGKDLVLQ
YTVKNXQKVDXGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVXGASKR---

---EIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDP
EAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNEL
YLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAK
AEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVIXRTLXK
*ix

Frame A:
gllvkkkiihiynyiffv**KRKNXXXRLLLGLIFLVFVFNFALSTVHFKDXFDNDWESR
WVVXDWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYXVSAKFPSFSXKGKDLVLQ
YTVKNXQKVDXGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVXGASKR---

---rdpsr*pr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*s
rscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*t
lpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrq
s*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n**qkrrsqkinqksxxtnfkx
ink

Frame B:
ayw*kkkssifiiisflfnkkerxpx*dfywv*fs*flfsilhyqqfiskxplitignqd
gllxigikkmvnqvn*filqvnglxmkikkvfklvkmlvstlyqlnsqvsvxkvki*fsn
tl*rmxkklixxvhtssyyhpn*inqhsmxnlniplclxqmfxxpqr---

---EIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDP
EAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNEL
YLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAK
AEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVIXRTLXK
*ix

Frame C:
ptgkkknhpyl*lylfclikkkexxxetfigfnflsfcfqfciinssfqrxl**rlgikm
gc*xla*rrw*ir*issycr*mvwx*kskrysn**rcsflxcis*ipkfqxqr*rfsspi
hcke*xks*lxxfihqvitiqirsisirwxi*ifhyvwxrclxxlke---

---rskqvtslmignyfhqnkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliq
klsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmns
tslmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppk
lkrerkltkn*lpkkllkkkpkklmkkkkkslkki*lklmtkkkksknqpkkxsxel*xn
k*x

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                       1053   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                       1053   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       1053   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                       1053   0.0
SFK591 (SFK591Q) /CSM/SF/SFK5-D/SFK591Q.Seq.d/                       1053   0.0
SFH171 (SFH171Q) /CSM/SF/SFH1-C/SFH171Q.Seq.d/                       1053   0.0
SFG873 (SFG873Q) /CSM/SF/SFG8-D/SFG873Q.Seq.d/                       1053   0.0
CHC137 (CHC137Q) /CSM/CH/CHC1-B/CHC137Q.Seq.d/                       1053   0.0
AFO794 (AFO794Q) /CSM/AF/AFO7-D/AFO794Q.Seq.d/                       1053   0.0
AFO349 (AFO349Q) /CSM/AF/AFO3-C/AFO349Q.Seq.d/                       1053   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1053   0.0    3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   3e-20  4
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    44   2e-12  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   9e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   2e-10  2
BT013428|BT013428.1 Lycopersicon esculentum clone 132068F, mRNA sequence.    74   4e-10  2
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74   6e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74   6e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74   6e-09  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    74   6e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   327   5e-88
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   204   5e-51
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   200   7e-50
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   197   5e-49
AK230218_1(AK230218|pid:none) Arabidopsis thaliana mRNA for hypo...   197   8e-49
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   196   2e-48
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   196   2e-48
AC149080_5(AC149080|pid:none) Medicago truncatula clone mth2-75j...   194   5e-48
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   194   5e-48
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   193   9e-48

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.38  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for AFI215 is exc

>AFI215F.Seq
GGCCTACTGGTAAAAAAAAAAATCATCCATATTTATAATTATATCTTTTTTGTTTAATAA
AAAAGAAAGAATNCCANCNTGAGACTTTTATTGGGTTTAATTTTCTTAGTTTTTGTTTTC
AATTTTGCATTATCAACAGTTCATTTCAAAGATNCCTTTGATAACGATTGGGAATCAAGA
TGGGTTGTTAGNGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCA
GGTAAATGGTTTGGNGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTC
TACNCTGTATCAGCTAAATTCCCAAGTTTCAGTANCAAAGGTAAAGATTTAGTTCTCCAA
TACACTGTAAAGAATGANCAAAAAGTTGATTGNGGNGGTTCATACATCAAGTTATTACCA
TCCAAATTAGATCAATCAGCATTCGATGGNGAATCTGAATATTCCATTATGTTTGGNCCA
GATGTTTGNGGNGCCTCAAAGAGA----------

>AFI215Z.Seq
----------AGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAA
AACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCG
ATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATC
CAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAA
CCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAAT
ACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAAC
TCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTT
CCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAA
AGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCA
AAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAG
AAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTG
ATGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCANACGAACTTTAAANA
AATAAATAAANA

>AFI215P.Seq
GGCCTACTGGTAAAAAAAAAAATCATCCATATTTATAATTATATCTTTTTTGTTTAATAA
AAAAGAAAGAATNCCANCNTGAGACTTTTATTGGGTTTAATTTTCTTAGTTTTTGTTTTC
AATTTTGCATTATCAACAGTTCATTTCAAAGATNCCTTTGATAACGATTGGGAATCAAGA
TGGGTTGTTAGNGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCA
GGTAAATGGTTTGGNGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTC
TACNCTGTATCAGCTAAATTCCCAAGTTTCAGTANCAAAGGTAAAGATTTAGTTCTCCAA
TACACTGTAAAGAATGANCAAAAAGTTGATTGNGGNGGTTCATACATCAAGTTATTACCA
TCCAAATTAGATCAATCAGCATTCGATGGNGAATCTGAATATTCCATTATGTTTGGNCCA
GATGTTTGNGGNGCCTCAAAGAGA----------AGAGATCCAAGCAGGTAACCTCGCTG
ATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCG
ATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACG
ATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAG
ACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAG
CAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACC
CAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCG
AATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCG
AAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAA
TGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAG
CTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCG
CTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAA
AAGTNATCANACGAACTTTAAANAAATAAATAAANA