>AFI214 (AFI214Q) /CSM/AF/AFI2-A/AFI214Q.Seq.d/
TTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAAAATAAAAAAATAATAAAGGATG
TTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATTCCTTGTATCAAAATCCTATGCG
ACGGATTGTAATAAAATTACTAATGAAGAAGAANGTCATAAATCTTCGGAATGTATTGTT
ATAAATTATACACCATGTTGNGGTGAACAAAAANGGGCTTGCTCTAAAGGTNCATTTGAT
ACTTGTACATATGAAAATAGTTGNTATAGAAATTCATCCAATAACCAAGTTGTTGAAGTA
TCAAATAAATGCTTCAATCTTGATGGATTTATTAAAATTACAACTCCAACCGAGTATTCT
TGTTCTGATGCCAAGATTAAAGAATGTGCCTTATTAGGTAAATXXXXXXXXXXAGTTTGC
TCTTTAAGATGTCCACCAAATTCATGAACGTAGATTTAATGACCAAGGTCATCAATGCTG
TGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCCACATGGCCATGAATGTAAAG
TTGATCACAATGGTAAAGAATGTTGCGTCAGATCCCATAGACCACCACCACCAGAAGTTT
GTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGATCATGGTAAAAAATGTT
GTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTGGTAGAGGTTTTGAATGTA
AAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAGACCACACCCACCACAAC
ATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTTGATCAACATGGAA
AAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCTTTAAGATGTCCACCAA
GACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGTTAAAGTTCACCACGATA
AATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGATCAACATGGAAAAGAAT
GTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCATTAAGATGTCCACCAAAACATG
AATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAGTAGAAATGATTGTTTAA
CTTGTGAAGACCTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATGAAAACTGTACCAA
TTATAAAGAAAATTGTTGNAAAAAGTTCCAGTATGTTATTCCACTATCCATTATTAGA

fklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEEXHKSSECIV
INYTPCXGEQKXACSKGXFDTCTYENSXYRNSSNNQVVEVSNKCFNLDGFIKITTPTEYS
CSDAKIKECALLGK---

---SLLFKMSTKFMNVDLMTKVINAV*rftmidvh*dvphgheckvdhngkeccvrshrp
pppevcslrcppkheckfddhgkkccvkihcdevcdldcgrgfeckirhdgskccvrser
phppqhekcnkrcppgheckvdqhgkeccvvahrpppkcslrcpprhecrvnhfgeeccv
kvhhdkcslrcppgheckvdqhgkeccvvahrpppkcslrcppkhecrinhfgeeccvks
rndcltcedlncerkglhcamktvpiikkivxksssmlfhypll

Frame A:
fklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEEXHKSSECIV
INYTPCXGEQKXACSKGXFDTCTYENSXYRNSSNNQVVEVSNKCFNLDGFIKITTPTEYS
CSDAKIKECALLGK---

---SLLFKMSTKFMNVDLMTKVINAV*rftmidvh*dvphgheckvdhngkeccvrshrp
pppevcslrcppkheckfddhgkkccvkihcdevcdldcgrgfeckirhdgskccvrser
phppqhekcnkrcppgheckvdqhgkeccvvahrpppkcslrcpprhecrvnhfgeeccv
kvhhdkcslrcppgheckvdqhgkeccvvahrpppkcslrcppkhecrinhfgeeccvks
rndcltcedlncerkglhcamktvpiikkivxksssmlfhypll

Frame B:
sn*kknnn*ls*k*knnkgccf*kileyfl*yslyqnpmrrivikllmkkxvinlrnvll
*iihhvxvnkxglalkvhlilvhmkivxieihpitkllkyqinasilmdllklqlqpsil
vlmprlknvpy*vn---

---vcslrcppns*t*i**prssmlckgsp**mfikmshmamnvklitmvknvasdpidh
hhqkfvh*dahqnmnvnlmimvknvv*rfivmkfvi*ivvevlnvkldmmvqnvvsvqkd
hthhnmknvirdahqamnvklinmeknvallpidhhqnal*dvhqdmnvesttlvknvvl
kfttinvh*dvhqamnvklinmeknvallpidhhqnah*dvhqnmnvesitlvknavlkv
emiv*lvkt*tvkekvyivp*klyql*rkllxkvpvcystihy*

Frame C:
qikkktitn*vknkkiikdvvskkywsifydipcikilcdgl**ny**rrxs*ifgmycy
klytmlx*tkxgll*rxi*ylyi*k*lx*kfiq*psc*sik*mlqs*wiy*nynsnrvfl
f*cqd*rmclir*---

---fal*dvhqiherrfndqghqccvkvhhdrcslrcptwp*m*s*sqw*rmlrqip*tt
ttrslfikmptkt*m*i**sw*kmlckdsl**sl*frlw*rf*m*n*t*wfkmlcpfrkt
tpttt*km**emptrp*m*s*stwkrmlrccp*tttkmlfkmstkt*m*sqplw*rmlc*
sspr*mfiemstrp*m*s*stwkrmlrccp*tttkmlikmstkt*m*nqslw*rmlc*k*
k*lfnl*rpkl*kkrftlchenctnykencxkkfqyviplsiir

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI214 (AFI214Q) /CSM/AF/AFI2-A/AFI214Q.Seq.d/                       2303   0.0
SFI216 (SFI216Q) /CSM/SF/SFI2-A/SFI216Q.Seq.d/                       1608   0.0
CHL853 (CHL853Q) /CSM/CH/CHL8-C/CHL853Q.Seq.d/                       1606   0.0
AHE229 (AHE229Q) /CSM/AH/AHE2-B/AHE229Q.Seq.d/                       1600   0.0
SFG663 (SFG663Q) /CSM/SF/SFG6-C/SFG663Q.Seq.d/                       1596   0.0
AHC814 (AHC814Q) /CSM/AH/AHC8-A/AHC814Q.Seq.d/                       1596   0.0
AHC488 (AHC488Q) /CSM/AH/AHC4-D/AHC488Q.Seq.d/                       1596   0.0
AFM858 (AFM858Q) /CSM/AF/AFM8-C/AFM858Q.Seq.d/                       1596   0.0
CHF544 (CHF544Q) /CSM/CH/CHF5-B/CHF544Q.Seq.d/                       1590   0.0
CHG879 (CHG879Q) /CSM/CH/CHG8-D/CHG879Q.Seq.d/                       1576   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.  1356   0.0    9
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.  1356   0.0    10
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.    66   6e-15  4
X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.    66   2e-14  4
AC124167|AC124167.2 Tetraodon nigroviridis clone GSTNB-42J2, WORKING DRAFT SEQUENCE, 14 unordered pieces.    38   5e-04  6
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    48   0.028  3
AC111000|AC111000.3 Homo sapiens BAC clone RP13-644M16 from 4, complete sequence.    32   0.090  10
AC127160|AC127160.3 Rattus norvegicus clone CH230-460D14, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    48   0.33   1
AC141955|AC141955.2 Rattus norvegicus clone CH230-418P10, WORKING DRAFT SEQUENCE, 29 unordered pieces.    48   0.33   1
AC084581|AC084581.1 Caenorhabditis briggsae cosmid G40L08, complete sequence.    38   0.36   4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   544   e-167
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   193   1e-47
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...   112   3e-23
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    79   2e-13
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        74   6e-13
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    70   1e-10
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    70   1e-10
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    67   2e-09
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    65   4e-09
FJ380206_1(FJ380206|pid:none) Haliotis discus discus antistasin-...    62   4e-08

 psg: 1.02  gvh: 0.68  alm: 0.47  top: 0.20  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for AFI214 is exc

>AFI214F.Seq
TTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAAAATAAAAAAATAATAAAGGATG
TTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATTCCTTGTATCAAAATCCTATGCG
ACGGATTGTAATAAAATTACTAATGAAGAAGAANGTCATAAATCTTCGGAATGTATTGTT
ATAAATTATACACCATGTTGNGGTGAACAAAAANGGGCTTGCTCTAAAGGTNCATTTGAT
ACTTGTACATATGAAAATAGTTGNTATAGAAATTCATCCAATAACCAAGTTGTTGAAGTA
TCAAATAAATGCTTCAATCTTGATGGATTTATTAAAATTACAACTCCAACCGAGTATTCT
TGTTCTGATGCCAAGATTAAAGAATGTGCCTTATTAGGTAAAT----------

>AFI214Z.Seq
----------AGTTTGCTCTTTAAGATGTCCACCAAATTCATGAACGTAGATTTAATGAC
CAAGGTCATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCCACA
TGGCCATGAATGTAAAGTTGATCACAATGGTAAAGAATGTTGCGTCAGATCCCATAGACC
ACCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGA
TCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTGG
TAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAG
ACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAA
AGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTC
TTTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGT
TAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGA
TCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCATTAAG
ATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAG
TAGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAAGGTTTACATTGTGC
CATGAAAACTGTACCAATTATAAAGAAAATTGTTGNAAAAAGTTCCAGTATGTTATTCCA
CTATCCATTATTAGA

>AFI214P.Seq
TTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAAAATAAAAAAATAATAAAGGATG
TTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATTCCTTGTATCAAAATCCTATGCG
ACGGATTGTAATAAAATTACTAATGAAGAAGAANGTCATAAATCTTCGGAATGTATTGTT
ATAAATTATACACCATGTTGNGGTGAACAAAAANGGGCTTGCTCTAAAGGTNCATTTGAT
ACTTGTACATATGAAAATAGTTGNTATAGAAATTCATCCAATAACCAAGTTGTTGAAGTA
TCAAATAAATGCTTCAATCTTGATGGATTTATTAAAATTACAACTCCAACCGAGTATTCT
TGTTCTGATGCCAAGATTAAAGAATGTGCCTTATTAGGTAAAT----------AGTTTGC
TCTTTAAGATGTCCACCAAATTCATGAACGTAGATTTAATGACCAAGGTCATCAATGCTG
TGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCCACATGGCCATGAATGTAAAG
TTGATCACAATGGTAAAGAATGTTGCGTCAGATCCCATAGACCACCACCACCAGAAGTTT
GTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGATCATGGTAAAAAATGTT
GTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTGGTAGAGGTTTTGAATGTA
AAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAGACCACACCCACCACAAC
ATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTTGATCAACATGGAA
AAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCTTTAAGATGTCCACCAA
GACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGTTAAAGTTCACCACGATA
AATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGATCAACATGGAAAAGAAT
GTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCATTAAGATGTCCACCAAAACATG
AATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAGTAGAAATGATTGTTTAA
CTTGTGAAGACCTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATGAAAACTGTACCAA
TTATAAAGAAAATTGTTGNAAAAAGTTCCAGTATGTTATTCCACTATCCATTATTAGA