>AFI211 (AFI211Q) /CSM/AF/AFI2-A/AFI211Q.Seq.d/
ACTGTTGGCCTACTGGTATTTTTTATTTATTTTTTTTACAAAGGGGAAAACAAAGATATT
GGTGTTTGGTCTTTCTATTGTTTTTCTTTGTAATAAATAACCTCAAACACCCACACACAA
AAACAAAAATAAACACAATGAAATCCATACCAATAATTTTATTAGTATTGATTGGATTAT
TATTAGCATCTGTATATTCCCATGACATTTTAATTACAGAAAAAGGACAAAAATCATTAG
ATAAATTAGATAAAGCAGCAGCAACAACTTTACAACATGGTGAAAACATTCAATTAAGCT
GTGATGTTTGTCAAATTGGTGCATCATTATTAGAAGATTTCATTAAAAAGAATGCTTCAT
TAACAGAAATAATTAAAGGTTTATCTGACCTTTGTATTGCCTCAAAAGAAGAACAACCAG
AAGTTTGTACAGGTATTTTAAATAATTATGTACCAATTATAGTTGATGTACTTATTCAAT
CAGATTTTACACCATCACAATTATGTGGATATTTTAAGATTTGTTCAGCAACAGGATCAT
CAGAATCATCATCAATTTCAAATTCATTTGATAATGAATATAGACAAGNAATCATTTAAT
XXXXXXXXXX

CWPTGIFYLFFLQRGKQRYWCLVFLLFFFVINNLKHPHTKTKINTMKSIPIILLVLIGLL
LASVYSHDILITEKGQKSLDKLDKAAATTLQHGENIQLSCDVCQIGASLLEDFIKKNASL
TEIIKGLSDLCIASKEEQPEVCTGILNNYVPIIVDVLIQSDFTPSQLCGYFKICSATGSS
ESSSISNSFDNEYRQXII*---

Frame A:
tvgllvffiyffykgenkdigvwsfycfsl**itsnthtqkqk*tq*npyq*fy*y*ldy
y*hlyipmtf*lqkkdknh*in*ikqqqqlynmvktfn*avmfvklvhhy*kislkrmlh
*qk*lkvyltfvlpqkknnqkfvqvf*iimyql*lmylfnqilhhhnyvdilrfvqqqdh
qnhhqfqihlimnidkxsf---


Frame B:
llaywyflfifftkgktkilvfglsivflcnk*pqtpthknknkhneihtnnfisidwii
isicifp*hfnyrkrtkiir*ir*sssnnfttw*khsikl*clsnwciiirrfh*kecfi
nrnn*rfi*plyclkrrttrslyryfk*lctnys*ctysirfytitimwif*dlfsnrii
riiinfkfi***i*txnhl---


Frame C:
CWPTGIFYLFFLQRGKQRYWCLVFLLFFFVINNLKHPHTKTKINTMKSIPIILLVLIGLL
LASVYSHDILITEKGQKSLDKLDKAAATTLQHGENIQLSCDVCQIGASLLEDFIKKNASL
TEIIKGLSDLCIASKEEQPEVCTGILNNYVPIIVDVLIQSDFTPSQLCGYFKICSATGSS
ESSSISNSFDNEYRQXII*---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI211 (AFI211Q) /CSM/AF/AFI2-A/AFI211Q.Seq.d/                        910   0.0
VHG223 (VHG223Q) /CSM/VH/VHG2-A/VHG223Q.Seq.d/                        884   0.0
VHC519 (VHC519Q) /CSM/VH/VHC5-A/VHC519Q.Seq.d/                        884   0.0
AFN745 (AFN745Q) /CSM/AF/AFN7-B/AFN745Q.Seq.d/                        739   0.0
VFH433 (VFH433Q) /CSM/VF/VFH4-B/VFH433Q.Seq.d/                        583   e-165
VFL234 (VFL234Q) /CSM/VF/VFL2-B/VFL234Q.Seq.d/                        456   e-127
VFH473 (VFH473Q) /CSM/VF/VFH4-D/VFH473Q.Seq.d/                        163   4e-39
SSG807 (SSG807Q) /CSM/SS/SSG8-A/SSG807Q.Seq.d/                         50   4e-05
SLC545 (SLC545Q) /CSM/SL/SLC5-B/SLC545Q.Seq.d/                         44   0.002
VHQ425 (VHQ425Q) /CSM/VH/VHQ4-B/VHQ425Q.Seq.d/                         40   0.038

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    30   3e-05  15
AL929354|AL929354.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 4/4.    36   8e-05  14
BX842642|BX842642.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 1/4.    32   2e-04  12
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    34   2e-04  13
AL590154|AL590154.19 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP71-74M9.    36   7e-04  8
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    32   8e-04  20
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    30   0.002  17
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    32   0.002  12
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    32   0.003  15
AC134887|AC134887.4 Oryza sativa chromosome 3 BAC OSJNBa0029P07 genomic sequence, complete sequence.    34   0.007  9

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

T27869(T27869)sphingomyelin phosphodiesterase (EC 3.1.4.12), aci...    54   3e-06
(Q23498) RecName: Full=Sphingomyelin phosphodiesterase 2;       ...    54   3e-06
U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...    52   2e-05
DQ062781_1(DQ062781|pid:none) Drosophila simulans saposin-relate...    48   2e-04
BC149798_1(BC149798|pid:none) Bos taurus prosaposin-like 1, mRNA...    46   7e-04
(P10960) RecName: Full=Sulfated glycoprotein 1;          Short=S...    46   7e-04
BC061759_1(BC061759|pid:none) Rattus norvegicus prosaposin, mRNA...    46   7e-04
(Q54C16) RecName: Full=Sphingomyelin phosphodiesterase B;       ...    46   7e-04
AK167719_1(AK167719|pid:none) Mus musculus 15 days pregnant adul...    46   9e-04
(Q61207) RecName: Full=Sulfated glycoprotein 1;          Short=S...    46   9e-04

 psg: 0.96  gvh: 0.52  alm: 0.17  top: 0.23  tms: 0.07  mit: 0.35  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.21  leu: 0.10  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFI211 is end

>AFI211F.Seq
ACTGTTGGCCTACTGGTATTTTTTATTTATTTTTTTTACAAAGGGGAAAACAAAGATATT
GGTGTTTGGTCTTTCTATTGTTTTTCTTTGTAATAAATAACCTCAAACACCCACACACAA
AAACAAAAATAAACACAATGAAATCCATACCAATAATTTTATTAGTATTGATTGGATTAT
TATTAGCATCTGTATATTCCCATGACATTTTAATTACAGAAAAAGGACAAAAATCATTAG
ATAAATTAGATAAAGCAGCAGCAACAACTTTACAACATGGTGAAAACATTCAATTAAGCT
GTGATGTTTGTCAAATTGGTGCATCATTATTAGAAGATTTCATTAAAAAGAATGCTTCAT
TAACAGAAATAATTAAAGGTTTATCTGACCTTTGTATTGCCTCAAAAGAAGAACAACCAG
AAGTTTGTACAGGTATTTTAAATAATTATGTACCAATTATAGTTGATGTACTTATTCAAT
CAGATTTTACACCATCACAATTATGTGGATATTTTAAGATTTGTTCAGCAACAGGATCAT
CAGAATCATCATCAATTTCAAATTCATTTGATAATGAATATAGACAAGNAATCATTTAAT
----------