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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI207
Library AF
(Link to library)
Clone ID AFI207
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14921-1
Original site URL
Representative seq. ID AFI207P
(Link to Original site)
Representative DNA sequence 
>AFI207 (AFI207Q) /CSM/AF/AFI2-A/AFI207Q.Seq.d/
CACTGTTGGCCTACTGGTNNTTAATAAAAAAAAAGAATGGGTCGTGAAATTATTTCAATT
CATATTGGTCAAGCAGGTGTCCAAGTAGGTAATTCATGTTGGGAACTTTACTGTTTAGAA
CATGGTATCGAACGTGATGGTTCAATTCCAGCTGATCGTAAACAATCAAGTGACGTCAAC
AACCTCAACAAAGATTTAGGTACATTCTTTAGCGAATCAACCAATGGTAAGAAAGTTGTC
CCAAGAGCCATCTTTTTAGATTTGGAACCAACCGTAATTGATGAAATTAGAACTGGTGAT
TACAAGAATCTCTTCCATCCAGAACAACTCATTACCGGTAAAGAAGATGCTGCCAACAAT
TATGCTCGTGGTCACTACACCGTTGGTAAAGAATTAATCGATGTTTGCGTCGATCGTATC
AGACGTTTAGCCGATCAATGTGATGGTCTCCAAGGTTTCCTCGTCTTCCACAGTGTTGGT
GGTGGTACAGGTTCTGGTTTCGGTTCTTTATTACTCCAAAAATTAGCTCTCGACTATGGT
GGTAAAAAATCAAAACTCGATTTCTGTGTTTACCCATCACCACAAGTTTCAACCTCTGTC
GTTGAACCATACAATTCTGTACTTTCAACTCATTCTTTATTAGAACATACTGATGTTAGT
XXXXXXXXXXGGTCAATTAAATCTTGATATCAATGATATTCAAACCAATTTAGTTCCATT
CCCACGTCTTCACTTTGTACTCTGCTCATATGCACCAGTTATCTCACGTGAAAAAGCTCA
CCACGAAACCATCACTGTCGATAACATCACCAGTGCCGTCTTCTCTGAAAAGAACATTAT
GGCCAAATGTCAACCAAATCTTGGTAAATATATGGCCTGTTGTTTAATGTACAGAGGTGA
TATCGTACCAAAAGAAGCCCAAAAAGCCGTTCAAAACATTCGTTCAGAGAAATCAAGAAA
CGTTTCCTTTGTCGATTGGTCCCCAACTGGTTTCAAATGTGGTATCAATAATCAAGCTCC
AGTTTCAACCAAAGATAGTGAAATGGCTGAAGTCAAGAAGAGCGTTTGTATGTTATCAAA
CACCACCGCCATCTCTCAAGTCTTCTCAAGAATCAACCACAAATTCGATCTCATGTTTGT
CAAGAGAGCTTTCGTCCATTGGTACGTTGGTGAAGGTATGGAGGAAGGTGAGTTTGCTGA
AGCTCGTGACGATTTACTTGCTCTCGAAAAAGATTATGAATCTGTTTCTGCCTCAACTGA
AGGTGAAGAACAAGGAGAAGAATATTAAACAATCACATCTTCTATGATAATATCAACTTT
AAACTGTTGTTATTCTTTCATAATAAAAATAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
hcwptgx**KKRMGREIISIHIGQAGVQVGNSCWELYCLEHGIERDGSIPADRKQSSDVN
NLNKDLGTFFSESTNGKKVVPRAIFLDLEPTVIDEIRTGDYKNLFHPEQLITGKEDAANN
YARGHYTVGKELIDVCVDRIRRLADQCDGLQGFLVFHSVGGGTGSGFGSLLLQKLALDYG
GKKSKLDFCVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVS---

---GQLNLDINDIQTNLVPFPRLHFVLCSYAPVISREKAHHETITVDNITSAVFSEKNIM
AKCQPNLGKYMACCLMYRGDIVPKEAQKAVQNIRSEKSRNVSFVDWSPTGFKCGINNQAP
VSTKDSEMAEVKKSVCMLSNTTAISQVFSRINHKFDLMFVKRAFVHWYVGEGMEEGEFAE
ARDDLLALEKDYESVSASTEGEEQGEEY*titssmiistlnccysfiiki
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgx**KKRMGREIISIHIGQAGVQVGNSCWELYCLEHGIERDGSIPADRKQSSDVN
NLNKDLGTFFSESTNGKKVVPRAIFLDLEPTVIDEIRTGDYKNLFHPEQLITGKEDAANN
YARGHYTVGKELIDVCVDRIRRLADQCDGLQGFLVFHSVGGGTGSGFGSLLLQKLALDYG
GKKSKLDFCVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVS---

---GQLNLDINDIQTNLVPFPRLHFVLCSYAPVISREKAHHETITVDNITSAVFSEKNIM
AKCQPNLGKYMACCLMYRGDIVPKEAQKAVQNIRSEKSRNVSFVDWSPTGFKCGINNQAP
VSTKDSEMAEVKKSVCMLSNTTAISQVFSRINHKFDLMFVKRAFVHWYVGEGMEEGEFAE
ARDDLLALEKDYESVSASTEGEEQGEEY*titssmiistlnccysfiiki

Frame B:
tvgllvxnkkkewvvklfqfilvkqvsk*vihvgnftv*nmvsnvmvqfqlivnnqvtst
tstki*vhslanqpmvrklsqepsf*iwnqp*lmklelvitrissiqnnslpvkkmlpti
mlvvttplvkn*smfasivsdv*pinvmvskvsssstvlvvvqvlvsvlyyskn*lstmv
vknqnsisvfthhhkfqplslnhtilyfqlily*nilml---

---vn*ilismifkpi*fhshvftlysahmhqlshvkklttkpslsitspvpsslkrtlw
pnvnqilvniwpvv*ctevisyqkkpkkpfktfvqrnqetfplsigpqlvsnvvsiiklq
fqpkivkwlksrrafvcyqtpppslkssqesttnsisclsrelssigtlvkvwrkvsllk
lvtiyllskkimnlflpqlkvknkeknikqshll**yql*tvvils**ky

Frame C:
llaywxlikkkngs*nyfnsywssrcpsr*fmlgtllfrtwyrt*wfnss*s*tik*rqq
pqqrfryil*rinqw*escpkshlfrfgtnrn**n*nw*lqeslpsrtthyr*rrccqql
cswslhrw*rinrclrrsyqtfsrsm*wsprfprlpqcwwwyrfwfrffitpkissrlww
*kiktrflclpittsfnlcr*tiqfctfnsffirty*c*---

---siks*yq*ysnqfssiptsslctllictsylt*kssprnhhcr*hhqcrll*kehyg
qmstksw*iygllfnvqr*yrtkrspksrskhsfreikkrflcrlvpnwfqmwyq*ssss
fnqr**ng*sqeerlyvikhhrhlssllknqpqirshvcqesfrplvrw*ryggr*vc*s
s*rftcsrkrl*icfcln*r*rtrrrilnnhifydninfklllffhnkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI207 (AFI207Q) /CSM/AF/AFI2-A/AFI207Q.Seq.d/                       2555   0.0
VFK580 (VFK580Q) /CSM/VF/VFK5-D/VFK580Q.Seq.d/                       1344   0.0
AFD240 (AFD240Q) /CSM/AF/AFD2-B/AFD240Q.Seq.d/                       1344   0.0
AFG495 (AFG495Q) /CSM/AF/AFG4-D/AFG495Q.Seq.d/                       1336   0.0
VFE293 (VFE293Q) /CSM/VF/VFE2-D/VFE293Q.Seq.d/                       1334   0.0
AFL867 (AFL867Q) /CSM/AF/AFL8-C/AFL867Q.Seq.d/                       1328   0.0
AFF423 (AFF423Q) /CSM/AF/AFF4-A/AFF423Q.Seq.d/                       1322   0.0
AFF464 (AFF464Q) /CSM/AF/AFF4-C/AFF464Q.Seq.d/                       1314   0.0
AFB268 (AFB268Q) /CSM/AF/AFB2-C/AFB268Q.Seq.d/                       1306   0.0
AFN792 (AFN792Q) /CSM/AF/AFN7-D/AFN792Q.Seq.d/                       1281   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L13999|L13999.1 Dictyostelium discoideum alpha tubulin (TUBA) gene, complete cds.  1344   0.0    1
AF276946|AF276946.1 Polysphondylium violaceum alpha-tubulin gene, partial cds.   133   e-122  8
X12562|X12562.1 Naegleria gruberi mRNA for alpha-tubulin (2).      92   1e-77  9
X12563|X12563.1 Naegleria gruberi gene for alpha-tubulin (3).      90   3e-75  9
X12561|X12561.1 Naegleria gruberi mRNA for alpha-tubulin (1).      90   9e-75  9
X81049|X81049.1 N.gruberi mRNA for alpha-tubulin.                  92   4e-74  8
AY101350|AY101350.1 Vorticella microstoma alpha-tubulin gene, partial cds.    86   4e-64  11
X12365|X12365.1 Stylonychia lemnae macronuclear DNA for alpha-2-tubulin.    90   2e-55  8
Z49855|Z49855.1 Zosterograptus sp. gene for alpha-tubulin (partial).    88   1e-52  8
Y10035|Y10035.1 H.cavicola gene encoding alpha-tubulin.           105   2e-52  7
dna update 2004. 6.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF255556_1(AF255556|pid:none) Notothenia coriiceps alpha tubulin...   271   e-132
(P32255) RecName: Full=Tubulin alpha chain; AltName: Full=Alpha-...   390   e-107
DQ099491_1(DQ099491|pid:none) Acanthamoeba polyphaga alpha tubul...   208   e-103
AM168454_1(AM168454|pid:none) Dictyostelium discoideum partial t...   339   1e-91
AJ576069_1(AJ576069|pid:none) Paramecium tetraurelia macronuclea...   187   2e-88
AM168470_1(AM168470|pid:none) Dictyostelium minutum partial tuba...   319   1e-85
AM168480_1(AM168480|pid:none) Dictyostelium mucoroides partial t...   319   2e-85
AM168478_1(AM168478|pid:none) Dictyostelium capitatum partial tu...   318   3e-85
AM168481_1(AM168481|pid:none) Dictyostelium brefeldianum partial...   315   2e-84
AM168488_1(AM168488|pid:none) Dictyostelium brunneum partial tub...   315   2e-84
protein update 2009. 4.22
PSORT 

 psg: 0.83  gvh: 0.35  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for AFI207 is nuc
5' end seq. ID AFI207F
5' end seq. 
>AFI207F.Seq
CACTGTTGGCCTACTGGTNNTTAATAAAAAAAAAGAATGGGTCGTGAAATTATTTCAATT
CATATTGGTCAAGCAGGTGTCCAAGTAGGTAATTCATGTTGGGAACTTTACTGTTTAGAA
CATGGTATCGAACGTGATGGTTCAATTCCAGCTGATCGTAAACAATCAAGTGACGTCAAC
AACCTCAACAAAGATTTAGGTACATTCTTTAGCGAATCAACCAATGGTAAGAAAGTTGTC
CCAAGAGCCATCTTTTTAGATTTGGAACCAACCGTAATTGATGAAATTAGAACTGGTGAT
TACAAGAATCTCTTCCATCCAGAACAACTCATTACCGGTAAAGAAGATGCTGCCAACAAT
TATGCTCGTGGTCACTACACCGTTGGTAAAGAATTAATCGATGTTTGCGTCGATCGTATC
AGACGTTTAGCCGATCAATGTGATGGTCTCCAAGGTTTCCTCGTCTTCCACAGTGTTGGT
GGTGGTACAGGTTCTGGTTTCGGTTCTTTATTACTCCAAAAATTAGCTCTCGACTATGGT
GGTAAAAAATCAAAACTCGATTTCTGTGTTTACCCATCACCACAAGTTTCAACCTCTGTC
GTTGAACCATACAATTCTGTACTTTCAACTCATTCTTTATTAGAACATACTGATGTTAGT
----------
Length of 5' end seq. 660
3' end seq. ID AFI207Z
3' end seq. 
>AFI207Z.Seq
----------GGTCAATTAAATCTTGATATCAATGATATTCAAACCAATTTAGTTCCATT
CCCACGTCTTCACTTTGTACTCTGCTCATATGCACCAGTTATCTCACGTGAAAAAGCTCA
CCACGAAACCATCACTGTCGATAACATCACCAGTGCCGTCTTCTCTGAAAAGAACATTAT
GGCCAAATGTCAACCAAATCTTGGTAAATATATGGCCTGTTGTTTAATGTACAGAGGTGA
TATCGTACCAAAAGAAGCCCAAAAAGCCGTTCAAAACATTCGTTCAGAGAAATCAAGAAA
CGTTTCCTTTGTCGATTGGTCCCCAACTGGTTTCAAATGTGGTATCAATAATCAAGCTCC
AGTTTCAACCAAAGATAGTGAAATGGCTGAAGTCAAGAAGAGCGTTTGTATGTTATCAAA
CACCACCGCCATCTCTCAAGTCTTCTCAAGAATCAACCACAAATTCGATCTCATGTTTGT
CAAGAGAGCTTTCGTCCATTGGTACGTTGGTGAAGGTATGGAGGAAGGTGAGTTTGCTGA
AGCTCGTGACGATTTACTTGCTCTCGAAAAAGATTATGAATCTGTTTCTGCCTCAACTGA
AGGTGAAGAACAAGGAGAAGAATATTAAACAATCACATCTTCTATGATAATATCAACTTT
AAACTGTTGTTATTCTTTCATAATAAAAATAT
Length of 3' end seq. 682
Connected seq. ID AFI207P
Connected seq. 
>AFI207P.Seq
CACTGTTGGCCTACTGGTNNTTAATAAAAAAAAAGAATGGGTCGTGAAATTATTTCAATT
CATATTGGTCAAGCAGGTGTCCAAGTAGGTAATTCATGTTGGGAACTTTACTGTTTAGAA
CATGGTATCGAACGTGATGGTTCAATTCCAGCTGATCGTAAACAATCAAGTGACGTCAAC
AACCTCAACAAAGATTTAGGTACATTCTTTAGCGAATCAACCAATGGTAAGAAAGTTGTC
CCAAGAGCCATCTTTTTAGATTTGGAACCAACCGTAATTGATGAAATTAGAACTGGTGAT
TACAAGAATCTCTTCCATCCAGAACAACTCATTACCGGTAAAGAAGATGCTGCCAACAAT
TATGCTCGTGGTCACTACACCGTTGGTAAAGAATTAATCGATGTTTGCGTCGATCGTATC
AGACGTTTAGCCGATCAATGTGATGGTCTCCAAGGTTTCCTCGTCTTCCACAGTGTTGGT
GGTGGTACAGGTTCTGGTTTCGGTTCTTTATTACTCCAAAAATTAGCTCTCGACTATGGT
GGTAAAAAATCAAAACTCGATTTCTGTGTTTACCCATCACCACAAGTTTCAACCTCTGTC
GTTGAACCATACAATTCTGTACTTTCAACTCATTCTTTATTAGAACATACTGATGTTAGT
----------GGTCAATTAAATCTTGATATCAATGATATTCAAACCAATTTAGTTCCATT
CCCACGTCTTCACTTTGTACTCTGCTCATATGCACCAGTTATCTCACGTGAAAAAGCTCA
CCACGAAACCATCACTGTCGATAACATCACCAGTGCCGTCTTCTCTGAAAAGAACATTAT
GGCCAAATGTCAACCAAATCTTGGTAAATATATGGCCTGTTGTTTAATGTACAGAGGTGA
TATCGTACCAAAAGAAGCCCAAAAAGCCGTTCAAAACATTCGTTCAGAGAAATCAAGAAA
CGTTTCCTTTGTCGATTGGTCCCCAACTGGTTTCAAATGTGGTATCAATAATCAAGCTCC
AGTTTCAACCAAAGATAGTGAAATGGCTGAAGTCAAGAAGAGCGTTTGTATGTTATCAAA
CACCACCGCCATCTCTCAAGTCTTCTCAAGAATCAACCACAAATTCGATCTCATGTTTGT
CAAGAGAGCTTTCGTCCATTGGTACGTTGGTGAAGGTATGGAGGAAGGTGAGTTTGCTGA
AGCTCGTGACGATTTACTTGCTCTCGAAAAAGATTATGAATCTGTTTCTGCCTCAACTGA
AGGTGAAGAACAAGGAGAAGAATATTAAACAATCACATCTTCTATGATAATATCAACTTT
AAACTGTTGTTATTCTTTCATAATAAAAATAT
Length of connected seq. 1342
Full length Seq ID -
Full length Seq. -
Length of full length seq. -