>AFI203 (AFI203Q) /CSM/AF/AFI2-A/AFI203Q.Seq.d/
XXXXXXXXXXCCNCAGTTNATCAAGCTTGTTTANATCANGGNATTTTCNTTTCCNANCAA
GTTAAAAAACCCGGNATTTTTGANATTGNTTANNANGGGACTGGGGCCCTTCCNGGGGCA
TGTTGGNAATTNAAAAAANANGCNAAAGNAAAATTCNATCNCNTTG

---XVXQACLXXGIFXSXQVKKPGIFXIXXXGTGALPGACWXXKKXAKXKFXXX

Frame A:
---xsxsslfxsxxfxfxxs*ktrxf*xxlxxdwgpsxgmlxixkxxxxkixsx


Frame B:
---XVXQACLXXGIFXSXQVKKPGIFXIXXXGTGALPGACWXXKKXAKXKFXXX


Frame C:
---qxiklvxixxfsfpxklknpxflxlxxxglgpfxghvgnxkxxxkxnsixl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI203 (AFI203Q) /CSM/AF/AFI2-A/AFI203Q.Seq.d/                        167   6e-41
SFD808 (SFD808Q) /CSM/SF/SFD8-A/SFD808Q.Seq.d/                        161   4e-39
CFI267 (CFI267Q) /CSM/CF/CFI2-C/CFI267Q.Seq.d/                        147   6e-35
CFA566 (CFA566Q) /CSM/CF/CFA5-C/CFA566Q.Seq.d/                        147   6e-35
SFD853 (SFD853Q) /CSM/SF/SFD8-C/SFD853Q.Seq.d/                        143   9e-34
CFJ285 (CFJ285Q) /CSM/CF/CFJ2-D/CFJ285Q.Seq.d/                        143   9e-34
AFI647 (AFI647Q) /CSM/AF/AFI6-B/AFI647Q.Seq.d/                        143   9e-34
AFI209 (AFI209Q) /CSM/AF/AFI2-A/AFI209Q.Seq.d/                        143   9e-34
AFC601 (AFC601Q) /CSM/AF/AFC6-A/AFC601Q.Seq.d/                        143   9e-34
AFL772 (AFL772Q) /CSM/AF/AFL7-C/AFL772Q.Seq.d/                        141   4e-33

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Hits to DB: 687,346
Number of Sequences: 35661683
Number of extensions: 687346
Number of successful extensions: 134984
Number of sequences better than 10.0: 0
length of query: 166
length of database: X,215,351,023
effective HSP length: 22
effective length of query: 144
effective length of database: W,430,793,997
effective search space: 5678034335568
effective search space used: 5678034335568
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FM178379_1197(FM178379|pid:none) Aliivibrio salmonicida LFI1238 ...    36   0.34
CP000020_1061(CP000020|pid:none) Vibrio fischeri ES114 chromosom...    35   0.59
CP001358_45(CP001358|pid:none) Desulfovibrio desulfuricans subsp...    34   1.3
CP000789_2953(CP000789|pid:none) Vibrio harveyi ATCC BAA-1116 ch...    33   3.8

 psg: 0.90  gvh: 0.50  alm: 0.46  top: 0.60  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for AFI203 is nuc

>AFI203Z.Seq
----------CCNCAGTTNATCAAGCTTGTTTANATCANGGNATTTTCNTTTCCNANCAA
GTTAAAAAACCCGGNATTTTTGANATTGNTTANNANGGGACTGGGGCCCTTCCNGGGGCA
TGTTGGNAATTNAAAAAANANGCNAAAGNAAAATTCNATCNCNTTG