>AFI191 (AFI191Q) /CSM/AF/AFI1-D/AFI191Q.Seq.d/
XXXXXXXXXXTTTAGTAATTGATAATTCAATGTTAGATAGTAAATGGTATGGAGAGACAG
AGAAAATGGTATCAGCTATGTTTTCAGTTGCCAAAAAGCTTCAACCAACAATTATATTTA
TAGATGAAATTGATTCAATGGTTTCTACTAGAGAAGATTCAGAAAATGAAACATCAAATA
GTAAAAAATCAATACTATTACAACATTGGGACGGTTTCTTTAGTTCAGGTAATGATAAAG
TTATTGTAATGGGTGCAACTAATAGACCAAATTCAATCGATTATGCATTCTTAAGAAGAT
TACCAAAACGTATAAAAGTTGATTTACCTGATAAAGACCAAAGAAAACATATTTTACAAA
TTGTAATTTAAACTTTTTAATTTATTTATTTATTTATTTCTTTATTTATTTATTTCTTTA
TTTCTTTATTTATTTATTTATTTATTTATTTATTAACCAACCAACAACACACACCATTAT
AAATTTTAAAATTTAAAGATGTTAGAATATCATGTAGAAAATGATTTTGATTATGATAAA
ATTGCAAATTTAACAAAAGGTTATTCAGGTTCAGATTTAAAGGAATTATGTAAAAAAGCT
TCAATGCGTTTTATGAGATACCGACCTAAAACCGATAATATATTTGACT

---LVIDNSMLDSKWYGETEKMVSAMFSVAKKLQPTIIFIDEIDSMVSTREDSENETSNS
KKSILLQHWDGFFSSGNDKVIVMGATNRPNSIDYAFLRRLPKRIKVDLPDKDQRKHILQI
VI*tf*fiylfislfiyffislfiylfiylltnqqhtpl*ilkfkdvriscrk*f*l**n
ckfnkrlfrfrfkgim*ksfnafyeipt*nr*yi*

Frame A:
---LVIDNSMLDSKWYGETEKMVSAMFSVAKKLQPTIIFIDEIDSMVSTREDSENETSNS
KKSILLQHWDGFFSSGNDKVIVMGATNRPNSIDYAFLRRLPKRIKVDLPDKDQRKHILQI
VI*tf*fiylfislfiyffislfiylfiylltnqqhtpl*ilkfkdvriscrk*f*l**n
ckfnkrlfrfrfkgim*ksfnafyeipt*nr*yi*


Frame B:
---**liiqc*ivngmerqrkwyqlcfqlpksfnqqlyl*mkliqwfllekiqkmkhqiv
knqyyynigtvslvqvmikll*wvqlidqiqsimhs*edyqnv*kliyliktkenifykl
*fklfnlfiylflylfislflylfiylfiy*ptnnthhykf*nlkmleyhvendfdydki
anltkgysgsdlkelckkasmrfmryrpktdnifd


Frame C:
---sn**fnvr**mvwrdrengisyvfscqkastnnyiyr*n*fngfy*rrfrk*nik**
kintittlgrfl*fr***sycngcn**tkfnrlcilkkitktyks*ft**rpkktyftnc
nlnfliylfiyffiylflyffiylfiylfinqptthtiinfki*rc*nim*kmilimikl
qi*qkviqvqi*rnyvkklqcvl*dtdlkpiiylt

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI191 (AFI191Q) /CSM/AF/AFI1-D/AFI191Q.Seq.d/                        702   0.0
SFE671 (SFE671Q) /CSM/SF/SFE6-C/SFE671Q.Seq.d/                        698   0.0
SFJ291 (SFJ291Q) /CSM/SF/SFJ2-D/SFJ291Q.Seq.d/                        690   0.0
SFD761 (SFD761Q) /CSM/SF/SFD7-C/SFD761Q.Seq.d/                        690   0.0
VFH694 (VFH694Q) /CSM/VF/VFH6-D/VFH694Q.Seq.d/                        676   0.0
CFG430 (CFG430Q) /CSM/CF/CFG4-B/CFG430Q.Seq.d/                        609   e-173
SFK615 (SFK615Q) /CSM/SF/SFK6-A/SFK615Q.Seq.d/                        541   e-153
CFH536 (CFH536Q) /CSM/CF/CFH5-B/CFH536Q.Seq.d/                        515   e-145
AFI353 (AFI353Q) /CSM/AF/AFI3-C/AFI353Q.Seq.d/                        339   3e-92
CFC467 (CFC467Q) /CSM/CF/CFC4-C/CFC467Q.Seq.d/                        280   3e-74

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.   696   0.0    8
AF083031|AF083031.2 Guillardia theta nucleomorph chromosome 3, complete sequence.    42   3e-07  11
Z69664|Z69664.1 Caenorhabditis elegans cosmid K04D7.               54   4e-06  5
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    32   6e-05  15
AP004174|AP004174.1 Mycoplasma penetrans HF-2 DNA, complete genome, section 5/5.    42   6e-05  12
AE017199|AE017199.1 Nanoarchaeum equitans Kin4-M, complete genome.    48   7e-05  2
AP003194|AP003194.2 Clostridium perfringens DNA, complete genome, section 10/10.    38   3e-04  11
AL445563|AL445563.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 1/3.    34   8e-04  14
BM159751|BM159751.1 EST562274 PyBS Plasmodium yoelii yoelii cDNA clone PYCJI28 5' end, mRNA sequence.    44   0.001  2
BX572092|BX572092.1 Prochlorococcus marinus MED4 complete genome; segment 3/5.    46   0.001  11

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AP005848_28(AP005848|pid:none) Oryza sativa Japonica Group genom...   111   3e-30
CP000583_184(CP000583|pid:none) Ostreococcus lucimarinus CCE9901...   122   6e-27
(P54815) RecName: Full=Protein MSP1 homolog;  &Z69664_4(Z69664|p...   121   2e-26
Z69664_5(Z69664|pid:none) Caenorhabditis elegans Cosmid K04D7, c...   121   2e-26
T23311(T23311)hypothetical protein K04D7.2 - Caenorhabditis eleg...   121   2e-26
CR954203_185(CR954203|pid:none) Ostreococcus tauri strain OTTH05...   119   7e-26
FB781580_1(FB781580|pid:none) Sequence 853 from Patent WO2008034...   119   9e-26
AE017344_396(AE017344|pid:none) Cryptococcus neoformans var. neo...   118   1e-25
(P28737) RecName: Full=Protein MSP1; AltName: Full=TAT-binding h...   118   2e-25
CR382129_901(CR382129|pid:none) Yarrowia lipolytica strain CLIB1...   117   2e-25

 psg: 0.75  gvh: 0.37  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFI191 is cyt

>AFI191Z.Seq
----------TTTAGTAATTGATAATTCAATGTTAGATAGTAAATGGTATGGAGAGACAG
AGAAAATGGTATCAGCTATGTTTTCAGTTGCCAAAAAGCTTCAACCAACAATTATATTTA
TAGATGAAATTGATTCAATGGTTTCTACTAGAGAAGATTCAGAAAATGAAACATCAAATA
GTAAAAAATCAATACTATTACAACATTGGGACGGTTTCTTTAGTTCAGGTAATGATAAAG
TTATTGTAATGGGTGCAACTAATAGACCAAATTCAATCGATTATGCATTCTTAAGAAGAT
TACCAAAACGTATAAAAGTTGATTTACCTGATAAAGACCAAAGAAAACATATTTTACAAA
TTGTAATTTAAACTTTTTAATTTATTTATTTATTTATTTCTTTATTTATTTATTTCTTTA
TTTCTTTATTTATTTATTTATTTATTTATTTATTAACCAACCAACAACACACACCATTAT
AAATTTTAAAATTTAAAGATGTTAGAATATCATGTAGAAAATGATTTTGATTATGATAAA
ATTGCAAATTTAACAAAAGGTTATTCAGGTTCAGATTTAAAGGAATTATGTAAAAAAGCT
TCAATGCGTTTTATGAGATACCGACCTAAAACCGATAATATATTTGACT