>SFE354 (SFE354Q) /CSM/SF/SFE3-C/SFE354Q.Seq.d/
ATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTA
TTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAA
GATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGAT
GGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAA
GGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTC
AGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGAT
TGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGT
GAATCTGAATATNCCATTATGTTNGGXXXXXXXXXXCCCAGCTTCAATCGTTGATCCAGA
AGCTGTCNAACCAGAGGATTGGAATGAAGAAGACGNCGGTGAATGGGAAGCCCCAACCAT
CGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAA
AGGTGAATGGGNACNCCCATTAATTGATAACCCAGAATACGCTGAAGNCAATGAACTCTA
CCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCAT
CTTTAACAATATGATCGTCNCCGACTCTGTCGAANAAGCTAAAGATTTCTNTGAAAAGAC
CTTTGTTGCCAACCAANAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGC
TGAANAGANAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGC
CGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGN
CAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTT

iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYXIMX---

---PASIVDPEAVXPEDWNEEDXGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWXXPLID
NPEYAEXNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEXAKDFXEKTFVANQXAEK
KMFDDLEAAKAEXXKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDXKKEEVKKST
KKV

Frame A:
iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYXIMX---

---psfnr*srscxtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin*
*prir*xq*tlpl**srcyrfrimas*iwfhl*qydrxrlcrxs*rfl*kdlccqpxsrk
env*rprsrqs*xxkes*rkiscrksc*krsrrs**rrrrsr*rrfs*n*xqkrrsqkin
qks

Frame B:
fiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikkm
vnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkkli
vvvhtssyyhpn*inqhsmvnlnxplcx---

---PASIVDPEAVXPEDWNEEDXGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWXXPLID
NPEYAEXNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEXAKDFXEKTFVANQXAEK
KMFDDLEAAKAEXXKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDXKKEEVKKST
KKV

Frame C:
l*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rrw
*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*l
wwfihqvitiqirsisirw*i*ixhyvx---

---qlqsliqklsnqrigmkktxvngkpqpspiqntkvngkqrksqiqntkvngxxh*li
tqntlkxmnstslmisvlsvsnygklnlvpslti*ssptlsxklkisxkrpllptxkpkr
kclttskppklxrxrkltkn*lpkkllkkkpkklmkkkkkslkki*lklmxkkkksknqp
kk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                        694   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                        694   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        694   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        694   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        694   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                        694   0.0
VFE671 (VFE671Q) /CSM/VF/VFE6-C/VFE671Q.Seq.d/                        694   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                        694   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                        694   0.0
VFD449 (VFD449Q) /CSM/VF/VFD4-C/VFD449Q.Seq.d/                        694   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   694   0.0    4
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   2e-14  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    68   6e-09  2
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    68   3e-07  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    68   3e-07  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    68   3e-07  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    68   3e-07  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    68   3e-07  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    68   3e-07  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    68   3e-07  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   242   1e-62
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   154   6e-36
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   153   1e-35
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   152   2e-35
EF084103_1(EF084103|pid:none) Picea sitchensis clone WS0274_F04 ...   152   2e-35
U66344_1(U66344|pid:none) Arabidopsis thaliana calreticulin (Crt...   151   3e-35
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   151   3e-35
AC003114_8(AC003114|pid:none) Arabidopsis thaliana chromosome 1 ...   151   3e-35
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   151   3e-35
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   151   4e-35

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for SFE354 is exc

>SFE354F.Seq
ATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTA
TTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAA
GATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGAT
GGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAA
GGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTC
AGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGAT
TGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGT
GAATCTGAATATNCCATTATGTTNGG----------

>SFE354Z.Seq
----------CCCAGCTTCAATCGTTGATCCAGAAGCTGTCNAACCAGAGGATTGGAATG
AAGAAGACGNCGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAAT
GGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACNCCCATTAATTG
ATAACCCAGAATACGCTGAAGNCAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCG
GTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACT
CTGTCGAANAAGCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAANAAGCCGAAA
AGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAANAGANAAAGAAAGCTGACGAAA
AATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAG
AAGTCGCTGAAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGAAGTCAAAAAATCAA
CCAAAAAAGTT

>SFE354P.Seq
ATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTA
TTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAA
GATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGAT
GGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAA
GGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTC
AGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGAT
TGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGT
GAATCTGAATATNCCATTATGTTNGG----------CCCAGCTTCAATCGTTGATCCAGA
AGCTGTCNAACCAGAGGATTGGAATGAAGAAGACGNCGGTGAATGGGAAGCCCCAACCAT
CGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAA
AGGTGAATGGGNACNCCCATTAATTGATAACCCAGAATACGCTGAAGNCAATGAACTCTA
CCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCAT
CTTTAACAATATGATCGTCNCCGACTCTGTCGAANAAGCTAAAGATTTCTNTGAAAAGAC
CTTTGTTGCCAACCAANAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGC
TGAANAGANAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGC
CGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGN
CAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTT