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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFE291
Library SF
(Link to library)
Clone ID SFE291
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13024-1
Original site URL
Representative seq. ID SFE291P
(Link to Original site)
Representative DNA sequence 
>SFE291 (SFE291Q) /CSM/SF/SFE2-D/SFE291Q.Seq.d/
TTGCTGGTACTCATAAATTAGTTGATGGTTCACAAATCATTGTAAACGGTAATGCTAATA
TCATCTCTGGTTTAACCTCAACTGGTTCAGCTCTTTCTCACTTTACTGTTGGTCCAACTG
GTAGCTGTGTCCTCGGTAATACCCCAATGCCAACTCTCCCATCATTCCCAACCAXXXXXX
XXXXGATTTCACTCATGGTACCATTCAAGGTGTTGGTGCTCTTAAAATGTGGTACTTGTA
ACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGTAACGTTAAAATGGCCGCAA
CTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATCACCACCTCAACCCAAACCT
CAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACCACCACTCAATCATCATCAA
GCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATCGTTTGTTCAGGTACTGCTG
ATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAATCAAGCTAAAATTGCCTCTG
GTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGTAATGTCTCCTCAGTTAGCT
GTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCCGGTGCTGCTGCTGCTGCTG
CCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCAATTGTAAATTCACCATGTA
ACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACTCCTCCTCTCCAATGACAAT
AAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCTAACCAATGTATTTGTCAAT
TAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAAACAATAAAAATTTACATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
AGTHKLVDGSQIIVNGNANIISGLTSTGSALSHFTVGPTGSCVLGNTPMPTLPSFPT---

---ISLMVPFKVLVLLKCGTCNFANKIAANLNIVGNVKMAATATVYANIDALMSITTSTQ
TSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNVVASEANQAKIA
SGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAAASKVWSIVNSP
CNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkhtlfnfktikiy
i
Translated Amino Acid sequence (All Frames) 
Frame A:
llvlin*lmvhksl*tvmlisslv*pqlvqlfltlllvqlvavssvipqcqlshhsqp--
-

---dfthgtiqgvgalkmwyl*lcq*drc*pqhcw*r*ngrncnclckh*cfnvnhhlnp
nlnlsiinqdcqr*dhhsiiikliiihl*tirsndrlfryc*ccw*pqcrclrsqss*nc
lwyciqihlspsc*r*clls*lshlkhccccstscrccccccpkscygccl*slvnckft
m*l*mwieeiin*fnssspmtin*nntthsyfslf*pmylsinlltf*thpfqf*nnknl
h

Frame B:
cwys*is*wftnhckr*c*yhlwfnlnwfssfslycwsnw*lcpr*ypnanspiipn---

---ISLMVPFKVLVLLKCGTCNFANKIAANLNIVGNVKMAATATVYANIDALMSITTSTQ
TSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNVVASEANQAKIA
SGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAAASKVWSIVNSP
CNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkhtlfnfktikiy
i

Frame C:
AGTHKLVDGSQIIVNGNANIISGLTSTGSALSHFTVGPTGSCVLGNTPMPTLPSFPT---

---fhswyhsrcwcs*nvvlvtlpirslltstllvtlkwpqlqlsmqtlml*cqsppqpk
pqslnhqprlstvrpplnhhqahhhpslnnsfq*sfvqvllmllvtsmslpqkpiklklp
lvlysnssqpqllkvmspqlavspqalllllnklpvlllllpkkllwllplkfgql*ihh
vtvnvd*rdykliqlllsndnklk*ynsflfffiltnvfvn*linflntpfsilkq*kft
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFE291 (SFE291Q) /CSM/SF/SFE2-D/SFE291Q.Seq.d/                       1158   0.0
SLE342 (SLE342Q) /CSM/SL/SLE3-B/SLE342Q.Seq.d/                        837   0.0
SLA101 (SLA101Q) /CSM/SL/SLA1-A/SLA101Q.Seq.d/                        837   0.0
SHL563 (SHL563Q) /CSM/SH/SHL5-C/SHL563Q.Seq.d/                        837   0.0
SHL325 (SHL325Q) /CSM/SH/SHL3-B/SHL325Q.Seq.d/                        837   0.0
SHL307 (SHL307Q) /CSM/SH/SHL3-A/SHL307Q.Seq.d/                        837   0.0
SHL203 (SHL203Q) /CSM/SH/SHL2-A/SHL203Q.Seq.d/                        837   0.0
SHK865 (SHK865Q) /CSM/SH/SHK8-C/SHK865Q.Seq.d/                        837   0.0
SHK762 (SHK762Q) /CSM/SH/SHK7-C/SHK762Q.Seq.d/                        837   0.0
SHK650 (SHK650Q) /CSM/SH/SHK6-C/SHK650Q.Seq.d/                        837   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83087|U83087.1 Dictyostelium discoideum rsc12 mRNA, partial cds.   773   0.0    4
AX344185|AX344185.1 Sequence 32 from Patent WO0200926.             40   0.002  5
AX251781|AX251781.1 Sequence 42 from Patent WO0168911.             40   0.002  5
AJ297856|AJ297856.1 Lucilia sericata partial bcd gene for bicoid protein.    50   0.053  1
BX649504|BX649504.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-242G21.    38   0.080  5
X00417|X00417.1 Tetrahymena H4-I gene and flanking regions.        34   0.13   3
AC022143|AC022143.7 Homo sapiens chromosome 19 clone CTD-2378H7, complete sequence.    48   0.21   1
AL395211|AL395211.1 T7 end of clone AR0AA022B11 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    48   0.21   1
AC008747|AC008747.5 Homo sapiens chromosome 19 clone CTD-2588C8, complete sequence.    48   0.21   1
AC113496|AC113496.3 Mus musculus clone RP23-364G24, WORKING DRAFT SEQUENCE, 29 unordered pieces.    48   0.21   1
dna update 2003.12.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83087_1(U83087|pid:none) Dictyostelium discoideum rsc12 mRNA, p...   230   2e-62
EU680851_1(EU680851|pid:none) Lolium perenne ice recrystallizati...    36   1.9
AE006641_2267(AE006641|pid:none) Sulfolobus solfataricus P2, com...    35   3.3
AY530539_1(AY530539|pid:none) Mimulus ringens deficiens (DEF) mR...    34   7.4
AY524011_1(AY524011|pid:none) Paulownia tomentosa deficiens (DEF...    33   9.6
protein update 2009. 5.23
PSORT 

 psg: 0.90  gvh: 0.63  alm: 0.32  top: 0.30  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: extracellular, including cell wall
	 24.0 %: cytoplasmic
	 12.0 %: mitochondrial
	 12.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for SFE291 is exc
5' end seq. ID SFE291F
5' end seq. 
>SFE291F.Seq
TTGCTGGTACTCATAAATTAGTTGATGGTTCACAAATCATTGTAAACGGTAATGCTAATA
TCATCTCTGGTTTAACCTCAACTGGTTCAGCTCTTTCTCACTTTACTGTTGGTCCAACTG
GTAGCTGTGTCCTCGGTAATACCCCAATGCCAACTCTCCCATCATTCCCAACCA------
----
Length of 5' end seq. 174
3' end seq. ID SFE291Z
3' end seq. 
>SFE291Z.Seq
----------GATTTCACTCATGGTACCATTCAAGGTGTTGGTGCTCTTAAAATGTGGTA
CTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGTAACGTTAAAATGG
CCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATCACCACCTCAACCC
AAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACCACCACTCAATCAT
CATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATCGTTTGTTCAGGTA
CTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAATCAAGCTAAAATTG
CCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGTAATGTCTCCTCAG
TTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCCGGTGCTGCTGCTG
CTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCAATTGTAAATTCAC
CATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACTCCTCCTCTCCAAT
GACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCTAACCAATGTATTT
GTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAAACAATAAAAATTT
ACATA
Length of 3' end seq. 715
Connected seq. ID SFE291P
Connected seq. 
>SFE291P.Seq
TTGCTGGTACTCATAAATTAGTTGATGGTTCACAAATCATTGTAAACGGTAATGCTAATA
TCATCTCTGGTTTAACCTCAACTGGTTCAGCTCTTTCTCACTTTACTGTTGGTCCAACTG
GTAGCTGTGTCCTCGGTAATACCCCAATGCCAACTCTCCCATCATTCCCAACCA------
----GATTTCACTCATGGTACCATTCAAGGTGTTGGTGCTCTTAAAATGTGGTACTTGTA
ACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGTAACGTTAAAATGGCCGCAA
CTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATCACCACCTCAACCCAAACCT
CAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACCACCACTCAATCATCATCAA
GCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATCGTTTGTTCAGGTACTGCTG
ATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAATCAAGCTAAAATTGCCTCTG
GTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGTAATGTCTCCTCAGTTAGCT
GTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCCGGTGCTGCTGCTGCTGCTG
CCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCAATTGTAAATTCACCATGTA
ACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACTCCTCCTCTCCAATGACAAT
AAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCTAACCAATGTATTTGTCAAT
TAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAAACAATAAAAATTTACATA
Length of connected seq. 889
Full length Seq ID -
Full length Seq. -
Length of full length seq. -