>AFI173 (AFI173Q) /CSM/AF/AFI1-D/AFI173Q.Seq.d/
XXXXXXXXXXAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATG
AAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATC
TCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCA
TTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTT
AAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACT
TTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGG
TGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGAC
GTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATC
GAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATC
TCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCT
GCCATTGAATTCAAATTACCATCATCTCAAACNGATNATAATGTGAATCTAAAAAAGATT
CTTCTGC

---IATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSF
DTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWC
GHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPIS
YEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDXNVNLKKILL

Frame A:
---nryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslqfr*krnlli
ryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmv
rslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnl
l*rskk*syelc*ihsr*rch*iqitiisnxx*ceskkdss


Frame B:
---IATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSF
DTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWC
GHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPIS
YEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDXNVNLKKILL


Frame C:
---slphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvtismkkkpshs
ipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhga
viarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqsl
mkvkemii*tllnsfkitlplnsnyhhlkxxim*i*krffc

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK855 (SFK855Q) /CSM/SF/SFK8-C/SFK855Q.Seq.d/                       1291   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1291   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1291   0.0
AFN712 (AFN712Q) /CSM/AF/AFN7-A/AFN712Q.Seq.d/                       1291   0.0
AFN679 (AFN679Q) /CSM/AF/AFN6-D/AFN679Q.Seq.d/                       1291   0.0
AFN674 (AFN674Q) /CSM/AF/AFN6-D/AFN674Q.Seq.d/                       1291   0.0
AFN461 (AFN461Q) /CSM/AF/AFN4-C/AFN461Q.Seq.d/                       1291   0.0
AFN128 (AFN128Q) /CSM/AF/AFN1-B/AFN128Q.Seq.d/                       1291   0.0
AFM768 (AFM768Q) /CSM/AF/AFM7-C/AFM768Q.Seq.d/                       1291   0.0
AFM464 (AFM464Q) /CSM/AF/AFM4-C/AFM464Q.Seq.d/                       1291   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   170   3e-41
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   167   2e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   166   7e-40
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   166   7e-40
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   160   2e-38
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   159   9e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   2e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   1e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   2e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   2e-35

 psg: 0.86  gvh: 0.36  alm: 0.43  top: 0.50  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFI173 is cyt

>AFI173Z.Seq
----------AAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATG
AAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATC
TCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCA
TTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTT
AAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACT
TTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGG
TGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGAC
GTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATC
GAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATC
TCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCT
GCCATTGAATTCAAATTACCATCATCTCAAACNGATNATAATGTGAATCTAAAAAAGATT
CTTCTGC