>AFI162 (AFI162Q) /CSM/AF/AFI1-C/AFI162Q.Seq.d/
XXXXXXXXXXATTGATTCATACATTATCACATGATGCACAATCACAGGCAGAAGGCACAT
CACCATATAGAAATTTATTTTTAGAGATTCAAAATTTAGTTTACAAATATAATAAAGAGG
CAGCAATGAACCCTGACCAAACTAGAGTATTACGTTTATGCATTCAATCATTCTCTTCAC
CACTTTGGTCCAACGATGAAGAGGGAGTCATAGAGTTTTTACATGCATTAAAGGGTTTGT
TACGTTCATCAGTTGCAA

---LIHTLSHDAQSQAEGTSPYRNLFLEIQNLVYKYNKEAAMNPDQTRVLRLCIQSFSSP
LWSNDEEGVIEFLHALKGLLRSSVA

Frame A:
---LIHTLSHDAQSQAEGTSPYRNLFLEIQNLVYKYNKEAAMNPDQTRVLRLCIQSFSSP
LWSNDEEGVIEFLHALKGLLRSSVA


Frame B:
---*fihyhmmhnhrqkahhhieiyf*rfki*ftniikrqq*tltkleyyvyafnhslhh
fgptmkres*sfymh*rvcyvhqlq


Frame C:
---dsyiit*ctitgrrhiti*kfifrdskfslqi**rgsnep*pn*sitfmhsiilftt
lvqr*rgshrvftcikgfvtfisc

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI162 (AFI162Q) /CSM/AF/AFI1-C/AFI162Q.Seq.d/                        492   e-138
AFF385 (AFF385Q) /CSM/AF/AFF3-D/AFF385Q.Seq.d/                        402   e-111
SLG878 (SLG878Q) /CSM/SL/SLG8-D/SLG878Q.Seq.d/                         82   5e-15
VHB893 (VHB893Q) /CSM/VH/VHB8-D/VHB893Q.Seq.d/                         38   0.062
SSL749 (SSL749Q) /CSM/SS/SSL7-C/SSL749Q.Seq.d/                         38   0.062
SSL692 (SSL692Q) /CSM/SS/SSL6-D/SSL692Q.Seq.d/                         38   0.062
SSL665 (SSL665Q) /CSM/SS/SSL6-C/SSL665Q.Seq.d/                         38   0.062
CHP333 (CHP333Q) /CSM/CH/CHP3-B/CHP333Q.Seq.d/                         38   0.062
VSI721 (VSI721Q) /CSM/VS/VSI7-A/VSI721Q.Seq.d/                         36   0.24
SSL876 (SSL876Q) /CSM/SS/SSL8-D/SSL876Q.Seq.d/                         36   0.24

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CL588737|CL588737.1 OB__Ba0089M22.r OB__Ba Oryza brachyantha genomic clone OB__Ba0089M22 3', genomic survey sequence.    48   0.063  1
CL577093|CL577093.1 OB__Ba0028G21.r OB__Ba Oryza brachyantha genomic clone OB__Ba0028G21 3', genomic survey sequence.    48   0.063  1
AZ534576|AZ534576.1 ENTBM76TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   0.063  1
CL593852|CL593852.1 OB__Ba0054G06.r OB__Ba Oryza brachyantha genomic clone OB__Ba0054G06 3', genomic survey sequence.    48   0.063  1
CL556850|CL556850.1 OB__Ba0008K22.f OB__Ba Oryza brachyantha genomic clone OB__Ba0008K22 5', genomic survey sequence.    48   0.063  1
CK387431|CK387431.1 L0915F04-5 NIA Mouse Newborn Kidney cDNA Library (Long) Mus musculus cDNA clone NIA:L0915F04 IMAGE:30001503 5', mRNA sequence.    40   0.14   2
CF733272|CF733272.1 UI-M-HB0-ckb-a-19-0-UI.r1 NIH_BMAP_HB0 Mus musculus cDNA clone IMAGE:30607794 5', mRNA sequence.    40   0.17   2
AC094826|AC094826.4 Rattus norvegicus clone CH230-2O13, WORKING DRAFT SEQUENCE, 1 ordered piece.    38   0.20   2
CF729355|CF729355.1 UI-M-HD0-cld-i-21-0-UI.r1 NIH_BMAP_HD0 Mus musculus cDNA clone IMAGE:30614900 5', mRNA sequence.    40   0.21   2
BI744702|BI744702.1 rk91c11.y1 Meloidogyne javanica egg pAMP1 v6 Chiapelli McCarter Meloidogyne javanica cDNA 5' similar to TR:Q9W3X9 Q9W3X9 CG3977 PROTEIN. ;, mRNA sequence.    46   0.25   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XS0) RecName: Full=Probable elongator complex protein 4;    ...   166   2e-40
(Q96EB1) RecName: Full=Elongator complex protein 4;          Sho...    45   0.001
AK304885_1(AK304885|pid:none) Homo sapiens cDNA FLJ61586 complet...    45   0.001
AX119069_1(AX119069|pid:none) Sequence 233 from Patent WO0129221.      45   0.001
BC158667_1(BC158667|pid:none) Rattus norvegicus similar to elong...    43   0.004
(Q5XG58) RecName: Full=Elongator complex protein 4;  &BC084604_1...    41   0.011
AK034040_1(AK034040|pid:none) Mus musculus adult male diencephal...    40   0.018
(Q9ER73) RecName: Full=Elongator complex protein 4; AltName: Ful...    40   0.018
AJ276004_1(AJ276004|pid:none) Mus musculus mRNA for Paxneb prote...    40   0.018
AL512589_12(AL512589|pid:none) Mouse DNA sequence from clone RP2...    40   0.018

 psg: 0.75  gvh: 0.57  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for AFI162 is nuc

>AFI162Z.Seq
----------ATTGATTCATACATTATCACATGATGCACAATCACAGGCAGAAGGCACAT
CACCATATAGAAATTTATTTTTAGAGATTCAAAATTTAGTTTACAAATATAATAAAGAGG
CAGCAATGAACCCTGACCAAACTAGAGTATTACGTTTATGCATTCAATCATTCTCTTCAC
CACTTTGGTCCAACGATGAAGAGGGAGTCATAGAGTTTTTACATGCATTAAAGGGTTTGT
TACGTTCATCAGTTGCAA