>AFI157 (AFI157Q) /CSM/AF/AFI1-C/AFI157Q.Seq.d/
XXXXXXXXXXGTGGAACATGTATCAATGCCCAATTGGATGGCCATGGTGGNGTTAAATTC
NCTAAANAAGGTGATTTCCNAACTTCANATTTANATAAACTTAGCTTTGANATTAAAACC
TCTGCTGANAGTAGTTCATTTGCAGTTTATTTTGGNGATGNTGCAACTAAAAACNTTGAC
CCNTTAGTAGCCNCAACTTGGACCNCTGTTGAATTGAGTGTTAAATNTTTAACAAATAAN
ACAGTTGAAGGTTNTANAACATTTTTCAATAATCAAGCTGATCCAATTAANATATGGTTA
NATAATATTAAATGGACANACNCAGATGATGCTCCTNTTGATCNACCNACAATGGATTTA
ATTGAAGTAAATAATNAACCNTCAACAACNTCAGGNACTGGAACCCCNTCAAGNAAACCN
TCATCTAGNAGTGGTGGNGTTAGTGGTGGTGGNATTGGNAGTGAAAGNTCNATCTATGGA
TTAAGTTCTGAAAACCNANAAAATCAAAGTGGACNTCATG

---GTCINAQLDGHGGVKFXKXGDFXTSXLXKLSFXIKTSAXSSSFAVYFGDXATKNXDP
LVAXTWTXVELSVKXLTNXTVEGXXTFFNNQADPIXIWLXNIKWTXXDDAPXDXPTMDLI
EVNNXPSTTSGTGTPSXKPSSXSGGVSGGGIGSEXSIYGLSSENXXNQSGXH

Frame A:
---wnmyqcpigwpwwx*ix*xr*fpnfxfx*t*l*x*nlc*x*ficslfwxxcn*kx*p
xssxnldxc*iec*xfnk*xs*rxxnifq*ss*sn*xmvx*y*mdxxr*csx*stxngfn
*sk*xtxnnxrxwnpxkxtxi*xwwx*wwwxwx*kxxlwikf*kpxkskwts


Frame B:
---GTCINAQLDGHGGVKFXKXGDFXTSXLXKLSFXIKTSAXSSSFAVYFGDXATKNXDP
LVAXTWTXVELSVKXLTNXTVEGXXTFFNNQADPIXIWLXNIKWTXXDDAPXDXPTMDLI
EVNNXPSTTSGTGTPSXKPSSXSGGVSGGGIGSEXSIYGLSSENXXNQSGXH


Frame C:
---ehvsmpnwmamvxlnslxkvisxlxixinlalxlkpllxvvhlqfilxmxqlktltx
**pqlgplln*vlnx*qixqlkvxxhfsiikliqlxygxiilngxtqmmlllixxqwi*l
k*ixnxqqxqxlepxqxnxhlxvvxlvvvxlxvkxxsmd*vlktxkikvdxm

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI157 (AFI157Q) /CSM/AF/AFI1-C/AFI157Q.Seq.d/                        767   0.0
SSF684 (SSF684Q) /CSM/SS/SSF6-D/SSF684Q.Seq.d/                        751   0.0
SLB724 (SLB724Q) /CSM/SL/SLB7-A/SLB724Q.Seq.d/                        751   0.0
AFG601 (AFG601Q) /CSM/AF/AFG6-A/AFG601Q.Seq.d/                        751   0.0
CHB187 (CHB187Q) /CSM/CH/CHB1-D/CHB187Q.Seq.d/                        743   0.0
AFG506 (AFG506Q) /CSM/AF/AFG5-A/AFG506Q.Seq.d/                        743   0.0
SLH350 (SLH350Q) /CSM/SL/SLH3-C/SLH350Q.Seq.d/                        739   0.0
SSH126 (SSH126Q) /CSM/SS/SSH1-B/SSH126Q.Seq.d/                        726   0.0
SSG547 (SSG547Q) /CSM/SS/SSG5-B/SSG547Q.Seq.d/                        726   0.0
CFH127 (CFH127Q) /CSM/CF/CFH1-B/CFH127Q.Seq.d/                        708   0.0

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Hits to DB: 4,852,376
Number of Sequences: 35661683
Number of extensions: 4852376
Number of successful extensions: 285134
Number of sequences better than 10.0: 1
length of query: 520
length of database: X,215,351,023
effective HSP length: 22
effective length of query: 498
effective length of database: W,430,793,997
effective search space: 19636535410506
effective search space used: 19636535410506
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 21 (42.1 bits)

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.

 ***** No hits found ******

Lambda     K      H
   0.316    0.135    0.422

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 435,234,997

 psg: 0.75  gvh: 0.57  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.06  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.50
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: Golgi
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFI157 is cyt

>AFI157Z.Seq
----------GTGGAACATGTATCAATGCCCAATTGGATGGCCATGGTGGNGTTAAATTC
NCTAAANAAGGTGATTTCCNAACTTCANATTTANATAAACTTAGCTTTGANATTAAAACC
TCTGCTGANAGTAGTTCATTTGCAGTTTATTTTGGNGATGNTGCAACTAAAAACNTTGAC
CCNTTAGTAGCCNCAACTTGGACCNCTGTTGAATTGAGTGTTAAATNTTTAACAAATAAN
ACAGTTGAAGGTTNTANAACATTTTTCAATAATCAAGCTGATCCAATTAANATATGGTTA
NATAATATTAAATGGACANACNCAGATGATGCTCCTNTTGATCNACCNACAATGGATTTA
ATTGAAGTAAATAATNAACCNTCAACAACNTCAGGNACTGGAACCCCNTCAAGNAAACCN
TCATCTAGNAGTGGTGGNGTTAGTGGTGGTGGNATTGGNAGTGAAAGNTCNATCTATGGA
TTAAGTTCTGAAAACCNANAAAATCAAAGTGGACNTCATG