>AFI152 (AFI152Q) /CSM/AF/AFI1-C/AFI152Q.Seq.d/
XXXXXXXXXXATCCTTTGAAATCGCTGAATCAATTTTGNGTCCAAATGCCTGATAATTCC
TTAGCTGGTCGTACAGTATCANCCGCTATTTCTTTAATTCCAAAAGAAAATTCAAATATT
GACCCACAATCAGTTTATAAATCACTTTCAACATTTGGTATAACCTTAAATGGTTTAAGA
TTAAAAGTTTTAAATGCATCAAGTTTAAAGACAATATCATTATTTGGTTTAGATAAAAAT
TTACCTGAGGAATATTTTAGAGATTATTTTTCAGAATCATTTGATCCAAATATNAAAGAT
CCAAAATTTTGTAGAATTACATTCTCATCAAAAGTTTATAAACCACATTTGATCGGTAAA
GAATCATCACACTCTGGTGTTGCACATATTACGGTTGGCAGTCATTTAGTTGCCATGAGA
GCAATAAAAGCATTGTCCAATTTACCAATTTCTAGTTGGGTGGGTATTAGTAGATACGCT
TNCACTGAAGGTTCTGCCGCTGCAGATTTAATCNCAATTCTTGAAAGAAGGAGACATAGA
CAAATGGATGATATTNAATTAATGAAAGATATNATCGGGTTAAAAGCAAAAGTTAAAGAG
CTGGAAATATGAAGTAAAGAAACATGACGCATTCAAAGAAANAGGGTTCAACCAAAAGA

---PLKSLNQFXVQMPDNSLAGRTVSXAISLIPKENSNIDPQSVYKSLSTFGITLNGLRL
KVLNASSLKTISLFGLDKNLPEEYFRDYFSESFDPNXKDPKFCRITFSSKVYKPHLIGKE
SSHSGVAHITVGSHLVAMRAIKALSNLPISSWVGISRYAXTEGSAAADLIXILERRRHRQ
MDDIXLMKDXIGLKAKVKELEI*sket*riqrxrvqpk

Frame A:
---sfeiaesilxpna**flswsysixryffnskrkfky*ptisl*itfniwynlkwfki
ksfkcikfkdniiiwfr*kft*gif*rlffrii*skyxrskil*nyiliksl*ttfdr*r
iitlwcctyygwqsfschesnksivqftnf*lggy**irxh*rfcrcrfnxns*kket*t
ng*yxineryxrvksks*ragnmk*rnmthskkxgstkr


Frame B:
---PLKSLNQFXVQMPDNSLAGRTVSXAISLIPKENSNIDPQSVYKSLSTFGITLNGLRL
KVLNASSLKTISLFGLDKNLPEEYFRDYFSESFDPNXKDPKFCRITFSSKVYKPHLIGKE
SSHSGVAHITVGSHLVAMRAIKALSNLPISSWVGISRYAXTEGSAAADLIXILERRRHRQ
MDDIXLMKDXIGLKAKVKELEI*sket*riqrxrvqpk


Frame C:
---l*nr*infxskcliip*lvvqyxplfl*fqkkiqilthnqfinhfqhlv*p*mv*d*
kf*mhqv*rqyhylv*ikiylrnileiifqnhliqixkiqnfvelhshqkfinhi*svkn
hhtlvlhilrlavi*lp*eq*khcpiyqflvgwvlvdtlxlkvlplqi*sqflkegdidk
wmixn**kixsg*kqklkswkyevkkhdafkexgfnqk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI152 (AFI152Q) /CSM/AF/AFI1-C/AFI152Q.Seq.d/                       1239   0.0
AFL266 (AFL266Q) /CSM/AF/AFL2-C/AFL266Q.Seq.d/                       1100   0.0
AFI693 (AFI693Q) /CSM/AF/AFI6-D/AFI693Q.Seq.d/                        987   0.0
AFG653 (AFG653Q) /CSM/AF/AFG6-C/AFG653Q.Seq.d/                        969   0.0
SLC243 (SLC243Q) /CSM/SL/SLC2-B/SLC243Q.Seq.d/                        900   0.0
VSJ790 (VSJ790Q) /CSM/VS/VSJ7-D/VSJ790Q.Seq.d/                         48   2e-04
SSL809 (SSL809Q) /CSM/SS/SSL8-A/SSL809Q.Seq.d/                         40   0.042
AFD452 (AFD452Q) /CSM/AF/AFD4-C/AFD452Q.Seq.d/                         40   0.042
SSI503 (SSI503Q) /CSM/SS/SSI5-A/SSI503Q.Seq.d/                         38   0.16
SSG729 (SSG729Q) /CSM/SS/SSG7-B/SSG729Q.Seq.d/                         38   0.16

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    32   0.035  12
AC078940|AC078940.4 Homo sapiens BAC clone RP11-144B8 from 2, complete sequence.    36   0.11   6
AC018996|AC018996.3 Homo sapiens clone RP11-659I4, WORKING DRAFT SEQUENCE, 28 unordered pieces.    34   0.13   7
AP003194|AP003194.2 Clostridium perfringens DNA, complete genome, section 10/10.    32   0.17   11
AC147714|AC147714.1 Medicago truncatula clone mth2-138b2, WORKING DRAFT SEQUENCE, 7 unordered pieces.    48   0.17   1
AC144730|AC144730.17 Medicago truncatula clone mth2-5j23, WORKING DRAFT SEQUENCE, 2 ordered pieces.    48   0.17   1
CR325687|CR325687.1 Medicago truncatula BAC ends cultivar Jemalong A17 of Medicago  truncatula.    48   0.17   1
AC146565|AC146565.17 Medicago truncatula clone mth2-71g17, WORKING DRAFT SEQUENCE, 9 unordered pieces.    48   0.17   1
AC145767|AC145767.23 Medicago truncatula clone mth2-11f1, WORKING DRAFT SEQUENCE, 2 ordered pieces.    48   0.17   1
CR301309|CR301309.1 Medicago truncatula BAC ends cultivar Jemalong A17 of Medicago  truncatula.    48   0.17   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE017226_883(AE017226|pid:none) Treponema denticola ATCC 35405, ...    36   1.1
CP001001_1634(CP001001|pid:none) Methylobacterium radiotolerans ...    33   5.4
CU638744_229(CU638744|pid:none) Podospora anserina genomic DNA c...    33   7.0
AM910986_112(AM910986|pid:none) Plasmodium knowlesi strain H chr...    33   9.2

 psg: 0.75  gvh: 0.40  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.05
 nuc: 0.05  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for AFI152 is nuc

>AFI152Z.Seq
----------ATCCTTTGAAATCGCTGAATCAATTTTGNGTCCAAATGCCTGATAATTCC
TTAGCTGGTCGTACAGTATCANCCGCTATTTCTTTAATTCCAAAAGAAAATTCAAATATT
GACCCACAATCAGTTTATAAATCACTTTCAACATTTGGTATAACCTTAAATGGTTTAAGA
TTAAAAGTTTTAAATGCATCAAGTTTAAAGACAATATCATTATTTGGTTTAGATAAAAAT
TTACCTGAGGAATATTTTAGAGATTATTTTTCAGAATCATTTGATCCAAATATNAAAGAT
CCAAAATTTTGTAGAATTACATTCTCATCAAAAGTTTATAAACCACATTTGATCGGTAAA
GAATCATCACACTCTGGTGTTGCACATATTACGGTTGGCAGTCATTTAGTTGCCATGAGA
GCAATAAAAGCATTGTCCAATTTACCAATTTCTAGTTGGGTGGGTATTAGTAGATACGCT
TNCACTGAAGGTTCTGCCGCTGCAGATTTAATCNCAATTCTTGAAAGAAGGAGACATAGA
CAAATGGATGATATTNAATTAATGAAAGATATNATCGGGTTAAAAGCAAAAGTTAAAGAG
CTGGAAATATGAAGTAAAGAAACATGACGCATTCAAAGAAANAGGGTTCAACCAAAAGA