>AFI147 (AFI147Q) /CSM/AF/AFI1-B/AFI147Q.Seq.d/
CACTCTACATANACAACACACATTGATAAAATAATAGTTTCTTTCTTTTTTTTTTTCTTT
TTTTTTTTTTTTTTTTAATNAAAAAAAAAATNAAAATTANTTTNAAAAAAAANGXXXXXX
XXXX

lyixnth**NNSFFLFFFLFFFFFLXKKKXKIXXKKX---

Frame A:
hstxtthidkiivsfffffffffff*xkkkxklxxkk---


Frame B:
tlhxqhtlik**flsfffsffffffnxkknxnxfxkk---


Frame C:
lyixnth**NNSFFLFFFLFFFFFLXKKKXKIXXKKX---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI147 (AFI147Q) /CSM/AF/AFI1-B/AFI147Q.Seq.d/                         84   5e-16
SSE472 (SSE472Q) /CSM/SS/SSE4-C/SSE472Q.Seq.d/                         30   6.7
SHL321 (SHL321Q) /CSM/SH/SHL3-A/SHL321Q.Seq.d/                         30   6.7
SHI779 (SHI779Q) /CSM/SH/SHI7-D/SHI779Q.Seq.d/                         30   6.7
SHI428 (SHI428Q) /CSM/SH/SHI4-B/SHI428Q.Seq.d/                         30   6.7
SHD250 (SHD250Q) /CSM/SH/SHD2-C/SHD250Q.Seq.d/                         30   6.7
FC-BE16 (FC-BE16Q) /CSM/FC/FC-BE/FC-BE16Q.Seq.d/                       30   6.7
CHN149 (CHN149Q) /CSM/CH/CHN1-C/CHN149Q.Seq.d/                         30   6.7
CHM468 (CHM468Q) /CSM/CH/CHM4-C/CHM468Q.Seq.d/                         30   6.7
CHH438 (CHH438Q) /CSM/CH/CHH4-B/CHH438Q.Seq.d/                         30   6.7

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    84   5e-13  1
AY392439|AY392439.1 Dictyostelium discoideum P-selectin gene, complete cds.    76   1e-10  1
BX294381|BX294381.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-235G13.    42   1.7    1
BX255891|BX255891.5 Zebrafish DNA sequence from clone DKEY-263P20 in linkage group 20.    42   1.7    1
BZ443340|BZ443340.1 BONMH24TR BO_1.6_2_KB_tot Brassica oleracea genomic clone BONMH24, DNA sequence.    42   1.7    1
AC021819|AC021819.2 Homo sapiens chromosome 9 clone RP11-38L20 map 9, LOW-PASS SEQUENCE SAMPLING.    34   3.2    2
AC122480|AC122480.3 Mus musculus chromosome 14 clone RP24-355B22, complete sequence.    40   6.7    1
AC146615|AC146615.2 Mus musculus BAC clone RP23-19K2 from chromosome 14, complete sequence.    40   6.7    1
BX470214|BX470214.6 Zebrafish DNA sequence from clone DKEY-27M7 in linkage group 18.    40   6.7    1
AQ560005|AQ560005.1 HS_2083_A1_G04_T7C CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2083 Col=7 Row=M, DNA sequence.    40   6.7    1

 ***** No hits found ******

Lambda     K      H
   0.310    0.122    0.315

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 58,267,415

 psg: 1.05  gvh: 0.52  alm: 0.32  top: 0.87  tms: 0.07  mit: 0.37  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFI147 is cyt

>AFI147F.Seq
CACTCTACATANACAACACACATTGATAAAATAATAGTTTCTTTCTTTTTTTTTTTCTTT
TTTTTTTTTTTTTTTTAATNAAAAAAAAAATNAAAATTANTTTNAAAAAAAANG------
----